Propidium iodide Hmoov, tsaus nti liab crystalline, nrog kev ua yeeb yam ntawm ntau dua lossis sib npaug ntawm HPLC, Soluble hauv dej, yog ib qho uas siv cov neeg nuclealescent fluorescent. Raws li ib qho analogue ntawm kev coj tsis ncaj ntawm kev coj tsis ncaj, nws tuaj yeem nkag los ntawm cov hauv paus kom ua tiav txoj kev khi nrog DNA. Nyob ruaj khov nyob rau hauv chav tsev kub thiab siab, nws yog ib qho me me molecule aromatic sib txuas. Nws tuaj yeem siv rau kev kuaj mob cell kuaj kom pom, kev soj ntsuam ntawm tes, thiab cov kab mob kev tshawb fawb. Tab sis feem ntau, nws siv yog txwv rau cov hlwb los yog infiltrated. Yog tias nyob hauv paus tau ntsib, lawv yuav nquag tshem tawm cov leeg, yog li cov dej cawv lossis aldehyde reagents feem ntau yog xaiv rau cov txheej txheem per fluation thiab txheej txheem txhim kho. Aldehydes feem ntau sib txuam nrog cov protein ntau thiab qee lub cim benzaldehyde tuaj yeem yog qhov kev xaiv tsim nyog dua.
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Tshuaj Cov Qauv |
C27H34I2N4 |
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Muaj pes tsawg leej |
668.09 |
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Molecular hnyav |
668.41 |
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m/z |
668.09 (100.0%), 669.09 (29.2%), 670.09 (4.1%), 669.08 (1.5%) |
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Kev Ntsuam Xyuas |
C, 48.52; H, 5.13; I, 37.97; N, 8.38 |
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Cia & Solubility
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chaw ntim khoom |
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Cov Ntaub Ntawv Solubility |
H2O: 5 mg / ml (7.48 mm), sonication thiab cua sov rau 60℃tau pom zoo. DMOs: 6.68 mg / ml (10 hli), sonication yog pom zoo. |
1.Tetection ntawm cell Apoptosis thiab necrosis
(1) Nws tuaj yeem nkag mus rau sab nraud tuag lossis puas cell li DNA, uas yog siv los txheeb xyuas thiab ua kom muaj zog thiab necrotic hlwb.
(2) hauv cov xim cytometry, nws feem ntau siv kom paub qhov txawv ntawm cov hlwb nyob, apoptotic hlwb, thiab necrotic hlwb.
2.ClL Kev Ntsuam Xyuas
(1) Tom qab nruj nreem khi rau DNA, nws fluorescence siv yog sib xws li DNA cov ntsiab lus.
(2) los ntawm kev ntsuas cov ntsiab lus DNA uas siv cov xim cytometry, suav nrog kev paub txog xov tooj cua ntawm G0 / G1 theem, thiab G2 / m theem.
3.Cell Nucleus Staining
(1) feem ntau siv rau fluorescence staining ntawm cell nuclei, tshwj xeeb tshaj yog nyob rau hauv keeb kwm kev ua, cytological, thiab pathological.
(2) Nws tuaj yeem siv los soj ntsuam cov morphology, loj, thiab ntau dua ntawm lub cell nuclei.
4.Research ntawm cov kab mob
(1) Qee zaum nws siv rau cov kab mob tshawb fawb, tab sis saib xyuas yuav tsum tau them rau qhov cuam tshuam ntawm cov kua qaub undacellular acids (Enta).
(2) Muaj qee kis, nws kuj tseem siv tau ua ke nrog lwm tus tuag kom tau ntsuas cov kab mob muaj tseeb.
5.Cov membrance kev qhia ncaj qha hauv cov nus cytometry
(1) Nws tsis tuaj yeem nkag mus rau lub hlwb uas muaj sia nyob, tab sis tuaj yeem nkag rau cov neeg tuag lossis tsis zoo ntawm tes.
(2) Yog li ntawd, nyob rau hauv cov nus cytometry, feem ntau nws yog feem ntau siv los ua qhov taw qhia ntawm Membrane Cell Cell.
6.Pov ntawv thov
(1) Nws kuj tseem siv tau rau nuclear sib hauv kev kawm xws li nyob rau hauv situ hybridization thiab immunohistochemistry.
(2) Muaj qee kis, nws kuj tuaj yeem siv los tshawb xyuas cov kev hloov pauv hauv cell membrane permeability.
Lwm yam kev siv ntawmPropidium iodide HmoovHauv kev sim ntawm tes suav nrog:
Kev Kuaj Cell:
Nws yog ib feem siv los tshawb xyuas cov kev sib tw ntawm tes vim tias nws tsis tuaj yeem nkag lub cell nucleic acids, thiab tuaj yeem nkag lub hlwb liab ua kom pom qhov txawv ntawm cov qe thiab cov hlwb tuag.
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Kev Ruaj Ntseg:
Piv rau qee qhov kev ua kom zoo li eb fluorescent xws li EB (kev nyab xeeb rau kev nyab xeeb vim eb yog cov paub muaj zog carcinogenic mutagen
02
Kev tshawb fawb txog ntawm tes Aptoptosis:
Feem ntau siv hauv kev sib xyaw ua ke nrog lwm tus tuag xws li Annexin V los ntes cov theem sib txawv ntawm cov mob celltosis. Annexin v ua ke nrog FITC tuaj yeem sau npe thaum ntxov apoptotic hlwb, thaum nws tuaj yeem sau cov ntawv sau qeeb apoptotic thiab necrotic hlwb.
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DNA Frandmentation Nrhiav tau:
Nyob rau hauv theem lig ntawm cell auptoptosis, nws tuaj yeem siv los tshawb xyuas DNA fragmentation, uas yog tus yam ntxwv hloov pauv ntawm lub cell lub plab.
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Tunel txoj kev:
Nws tuaj yeem siv rau hauv tunel (davhlau ya nyob twg deoxysomlyl hloov mekuveidly mediated sau) kev sim los tshawb xyuas DNA strand tawg thaum lub cond mob.
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Txheeb xyuas ntawm lub xov tooj ntawm tes:
Yuav siv tau rau kev txheeb xyuas lub voj voog ntawm tes hauv cov nus cytometry, txheeb xyuas cov theem ntawm lub voj voog ntawm cov ntsiab lus DNA.
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Chromosomal thiab DNA cim:
Siv rau sau npe chromosomes lossis DNA, saib xyuas thiab txheeb xyuas cov txheej txheem DNA xws li DNA ua kev hloov pauv DNA, sau ntawv.
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Organelle Labelling:
Nws tuaj yeem siv tau los sau npe thiab pom cov koom ua ke xws li Mitochondria thiab Lysosomes hauv cov hlwb ntawm cov wavelength.
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Daim ntaub ntawv Nuroscience:
Nws yog siv nyob rau hauv neuroscience rau daim ntawv cim nurons thiab synapseses, saib cov kev sib kis ntawm neural cov cim, thiab lwm yam.
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Kev Ntsuam Xyuas Gene Kev Tshawb Xyuas
Nws tuaj yeem txheeb xyuas cov theem kev qhia ntawm cov noob thiab lawv cov kev faib tawm hauv cov hlwb los ntawm Labed Gene cov khoom lag luam.
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1.Cell membrane tob tob:
Propidium iodide Hmoovtsis tuaj yeem nkag mus rau hauv lub cev nqaij daim tawv Cell, tab sis tuaj yeem ua rau lub cell membrane ntawm cov hlwb tuag thiab lig theem apoptotic hlwb. Tus xeeb ceem no ua rau nws muaj qhov zas zoo tshaj plaws rau kev paub qhov txawv ntawm cov hlwb thiab cov hlwb tuag.
2.Fluorescence Kev Siv Txhim Kho:
Tom qab khi nrog nucleic acid, kev siv roj fluorescence tau nce li ntawm 30-40 nm, thaum lub siab tshaj plaws emissen wavelength hloov mus rau 15 nm qhov no ua rau nws rhiab heev thaum nrhiav tau.
3.Txhua yam ntxwv:
Nws qhov siab tshaj plaws / emission yoj yuav dhau 535/617 nm, uas ua rau nws muaj kev ntxub ntxaug zoo nyob rau hauv ntau} 2}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}} Cov xim fluorescination hauv kev sib xyaw nrog lwm cov fluorescent dyes.
4.Binding nrog DNA thiab RNA:
Nws tuaj yeem khi rau RNA thiab yuav tsum tau kho NucLease kev kho kom paub qhov txawv DNA los ntawm RNNing Staining
Qhov no muab kev yooj yim rau kev kawm RNA.
5.puility:
Peb muab ntau yam ntawm sprockets, cov menyuam yaus sib tw, lub zog, cov khaub ncaws, cov tes tsho, cov plaub hau, cov kabmob cov kabmob, thiab ntau yam.
6.Cov neeg:
Piv rau lwm tus neeg ua kom muaj kev lom zem xws li EB (kev nyab xeeb bromide vim eb yog cov paub muaj zog carcinogenic mutagen.
7.Widely siv:
Haum rau ntau cov kev sim tswv yim xws li fluorescence allcopy, microscal tsom iav, ntws cytometry, thiab fluorescence analalyzer tsom xam.
8.Txhais tus phooj ywg:
Nws cov khib nyiab tuaj yeem ua ncaj qha ua tiav yam tsis muaj kev ua qias tuaj rau ib puag ncig, uas ua tau raws li kev tiv thaiv niaj hnub.
Cov tshuaj tiv thaiv tsis zoo
Propidium iodide Hmoov(Pi) yog fluorescent daj dav siv hauv Cell Kev Tshawb Fawb Biology. Nws cov tub ntxhais muaj nuj nqi yog kom sau cov hlwb tuag los ntawm emritting liab fluoreesx 535 nm, emission wavelengthenthethung 617 nm) ntawm puag ncig ntawm ob leeg stranded DNA. Vim nws tsis muaj peev xwm nkag mus rau hauv cov cuab yeej ntawm tes, PI tau dhau los ua cov duab cytometry, thiab yog tsom rau cov tshuaj fluorometry, thiab tsom iav ntsej muag, thiab tsom mus rau hauv lub cev. Txawm li cas los xij, raws li cov tshuaj lom neeg siv tshuaj lom neeg muaj peev xwm ua rau muaj kev pheej hmoo rau tib neeg kev noj qab haus huv thiab ib puag ncig yog tias siv tsis raug cai lossis tsis muaj kev tiv thaiv.
Cov kev tshwm sim uas cuam tshuam ntsig txog kev sib chwv tawv nqaij
Kev sib chwv ncaj qha ntawm PI hmoov lossis nws cov kev daws teeb meem thiab cov tawv nqaij yuav ua rau muaj kev tsis haum, khaus, hlawv rau ntawm daim tawv nqaij. Qhov kev tawm tsam no feem ntau yog tshwm sim los ntawm cov nyhuv ncaj qha ntawm PI ntawm Stratum Corneum ntawm daim tawv nqaij. Lub sij hawm ntev los yog rov kis tau tus kheej tuaj yeem ua rau cov tawv nqaij puas tsuaj, ua rau muaj kev cuam tshuam rau hauv zos thiab ua rau muaj kev hu rau dermatitis. Yog li ntawd, thaum tuav hnab looj tes lossis kev daws teeb meem, tshuaj lom neeg resistant (xws li nitrile roj hmab hnab looj tes) yuav tsum tau hnav kom tsis txhob kov cov tawv nqaij. Yog tias muaj kev sib cuag nrog daim tawv nqaij, tam sim ntawd yaug qhov chaw sib tiv tauj nrog dej ntau thiab siv cov khaus loj los daws kev khaus khaus.
Cov kev tawm tsam cuam tshuam ntsig txog qhov muag hu
PI muaj ib qho kev cuam tshuam loj dua rau lub qhov muag thiab tej zaum yuav ua rau muaj kab mob qhov muag xws li mob caj dab thiab Keratitis. Cov tsos mob muaj xws li redness, o, mob, pearing, thiab tej zaum muaj qhov pom kev yuav cuam tshuam rau lub zeem muag. Thaum tuav Pi hmoov los yog kev daws teeb meem, cov duab tiv thaiv lossis ntsej muag yuav tsum tau hnav kom tiv thaiv cov hmoov lossis daws los ntawm kev sib cais mus rau hauv lub qhov muag. Yog tias muaj kev sib raug zoo nrog lub qhov muag, yaug tam sim ntawd nrog dej ntau lossis cov tshuaj qab ntsev tsawg kawg yog 15 feeb thiab nrhiav kev kho mob sai li sai tau.
Inhalation ntsig txog cov nyom twg
Tom qab nqus tau pi hmoov lossis cov khoom siv aerosol, nws yuav ua rau cov tsos mob xws li hnoos, mob hauv plab, thiab lub hauv siab nruj me ntsis. Lub sijhawm ntev lossis rov ua pa yuav ua rau muaj kev pheej hmoo ua pa thiab txawm tias muaj kev cuam tshuam ntev ntawm lub ntsws. Thaum tuav Pi hmoov, nws yuav tsum tau ua nyob rau hauv ib puag ncig muaj kev ua pa zoo, xws li siv lub ntsej muag fum lossis tso sab hauv zos. Hnav daim npog ntsej muag lossis nqus dej los tiv thaiv hmoov lossis aerosol hais los ntawm kev nqus tau. Tsis tu ncua Lung kev ntsuam xyuas, tshwj xeeb tshaj yog rau cov neeg ua haujlwm uas tau raug PI tau ntev ntev.
Cov kev tawm tsam cuam tshuam ntsig txog kev noj khoom haus tsis zoo
Txawm hais tias PI feem ntau yog siv rau hauv kev sim vitro, kev noj khoom haus kom tsis txhob noj yuav tshwm sim, tshwj xeeb yog nyob rau menyuam yaus lossis sim tsiaj. PI tej zaum yuav muaj kev cuam tshuam txog kev txhawb nqa ntawm lub plab zom mov ntawm cov plab zom mov, ua rau pom cov tsos mob xws li xeev siab, mob plab, thiab raws plab, thiab raws plab, thiab raws plab, thiab raws plab, thiab raws plab, thiab raws plab. Thaum muaj mob hnyav, tshuaj lom neeg mob ntsws los yog nkag rau hauv dej num yuav tshwm sim vim muaj ntau dhau. Khaws pi hmoov los yog daws kom ncav cuag cov menyuam yaus kom tsis txhob haus. Kev sim cov tsiaj yuav tsum raug saib xyuas nruj me ntsis thaum raug pi kom tiv thaiv kev siv tsis pub muaj kev sib tsoo. Yog tias kev noj khoom haus, nrhiav kev pab kho mob tam sim ntawd thiab tau txais kev kho mob raws li cov tsos mob.
Cim npe nrov: Propidium Iodide Hmoov Cas 25535-16-4, Tuam Tshoj Chaw Tsim Tshuaj, Chaw Tsim Tshuaj, Hoobkas, Yuav, Nqe, loj, rau muag