Shaanxi BLOOM Tech Co., Ltd. yog ib qho ntawm cov tuam txhab kev paub ntau tshaj plaws thiab cov neeg muag khoom ntawm cellulase hmoov cas 9012-54-8 hauv Suav teb. Txais tos rau cov lag luam wholesale bulk zoo cellulase hmoov cas 9012-54-8 muag ntawm no los ntawm peb lub hoobkas. Kev pabcuam zoo thiab tus nqi tsim nyog muaj.
Cellulase hmoov, ib qho kev sib xyaw enzyme tau muab los ntawm microbial fermentation (xws li Trichoderma thiab Aspergillus), yog lub ntsiab lus sib koom ua ke rau biocatalysts uas tshwj xeeb hydrolyze cov -1,4-glycosidic bonds ntawm cellulose. Nws tuaj yeem suav tias yog "tus kws tshaj lij hauv kev tshem tawm cov phab ntsa ntawm tes". Nws cov tsos yog ib qho zoo, lub teeb grey rau brownish- cov hmoov daj, uas muaj lub zog sib xyaw ua ke ntawm endo-glucanase, exo-glucanase, thiab -glucosidase, uas tuaj yeem "txiav" cov tawv tawv cellulose macromolecules rau hauv soluble oligosaccharides, glucose.fructose, thiab dissoluble. Nws txoj haujlwm tsis yog lub cev thickening lossis ncua kev kawm, tab sis ua rau lub hauv paus hloov pauv ntawm cov qauv tshuaj ntawm cov substrate los ntawm kev siv tshuaj lom neeg catalysis. Nyob rau hauv qhov mob me me (qhov kub thiab pH), nws ua rau cov txheej txheem nruj ntawm phab ntsa ntawm cov nroj tsuag, yog li tso cov khoom noj khoom haus encapsulated.
|
Qhov ntom |
1.2 g / ml ntawm 25 ° C |
|
Cia tej yam kev mob |
2-8 ° C |
|
Solubility deionized dej |
soluble5.0mg/mL (Sterile; Nyob rau hauv lub xub ntiag ntawm 0.15% polyhexamethylene biguanide (PHMB). |
|
Daim ntawv |
hmoov |
|
Xim |
dawb |
|
Soluble |
hauv dej txias |
|
Hazard symbol (GHS) |
GHS08 |
|
Cov lus ceeb toom |
Kev phom sij |
|
Kev piav qhia txog phom sij |
H334 |
|
Cov lus qhia ceev faj |
P261-P342+P311 |
|
Dangerous goods sign Xn |
Xis |
|
Hazard Category Code |
42 |
|
Cov lus qhia kev nyab xeeb |
22-24-36/37-2-45-23
|
|
WGK Germany |
1 |
|
RTECS No. |
FJ5375000 |
Cov yam ntxwv no ua rau nws yog ib qho cuab yeej muaj zog hauv kev lag luam zaub mov kom nce cov kua txiv hmab txiv ntoo thiab kev pom tseeb, hauv kev lag luam brewing rau kev txhawb cov hmoov txhuv nplej siab thiab tsw tsim, thiab hauv kev lag luam pub rau kev rhuav tshem cov khoom noj khoom haus thiab kev txhim kho kev zom zaub mov zoo. Tsis tas li ntawd, hauv textile bio- polishing, kev tshem tawm cov khoom siv tau zoo los ntawm cov tshuaj suav tshuaj, thiab kev siv cov peev txheej ntawm kev ua liaj ua teb pov tseg, nws qhia tau tias muaj peev xwm loj ntawm kev tsim tshuaj ntsuab ntsuab hauv ib puag ncig tus phooj ywg. Cov hmoov zoo li no yog qhov tseem ceeb ntsuab txuas ntxiv cov peev txheej cellulose thiab siab - cov txheej txheem lom neeg hloov pauv, thiab yog tus qauv ntawm biotechnology txhawb kev lag luam ib txwm muaj.
1. Cov ntawv thov kev ua liaj ua teb
Daim ntawv thov ntawmCellulase hmoovhauv kev ua liaj ua teb thiab nqaij qaib:
Cov tsiaj pub noj xws li nplej, taum, nplej, thiab ua los ntawm - cov khoom txhua tus muaj ntau cov cellulose. Tsuas yog cov ruminants, uas tuaj yeem siv ib feem ntawm rumen microbiota, lwm yam tsiaj txhu xws li npua thiab qaib tsis tuaj yeem siv cellulose.
2. Noj nyuj
Jiao Pinglin et al. (1996) tau ua ib qho kev sim nyuj castrated, ntxiv 40g ntawm cellulase ib lub taub hau ib hnub twg rau kev noj haus thiab pub rau 60 hnub. Cov txiaj ntsig tau pom tias pawg enzyme ntxiv muaj qhov hnyav txhua hnub ntawm 892.78g, thaum pawg tswj hwm muaj qhov hnyav txhua hnub ntawm 746.8g, nrog qhov sib txawv tseem ceeb (P<0.01). Jiaopinglin conducted an experiment using 30 Holstein cows. The experimental group added 50g of cellulase per cow per day.
Cov txiaj ntsig tau pom tias tag nrho cov kua mis ntawm 15 tus nyuj hauv pawg sim yog 2916kg ntawm 68 hnub, thaum tag nrho cov kua mis ntawm 15 tus nyuj hauv pawg tswj yog 2689kg ntawm 68 hnub, nrog qhov sib txawv tseem ceeb (P<0.05). Fu Liansheng et al. (1998) reported that under normal rumen function, after feeding cellulase for 5 days, the dry matter in the feces of adult cows and breeding cows decreased by 30% compared to before feeding. One week later, the ammonia content in the closed cowshed decreased by about 70%, the roughage intake increased by 8-10%, and the urea in urine decreased by 58.9%.
Cov nyuj cev xeeb tub tau pub cellulase los ntawm 30 hnub ua ntej xa khoom, thiab tsis muaj teeb meem kev zom zaub mov tom qab yug menyuam. Lub fetus hnyav nce los ntawm 1.5-3kg yam tsis muaj deformities lossis tsis muaj zog fetuses. Lub cev rov qab los ntawm nyuj yog ceev, thiab lub siab tshaj plaws cov kua mis yuav kav ntev (txog rau plaub lub hlis lactation). Zhao Changyou et al. (1998) tshuaj xyuas daim ntawv thov ntawm cellulase nyob rau hauv herbivorous tsiaj noj, uas ua tiav cov txiaj ntsig tseem ceeb.
3. Nqaij qaib
Kev noj zaub mov rau qaib broiler feem ntau yog noj ntses, pob kws, thiab taum noj mov. Txhawm rau txo qis kev siv cov khoom siv raw li no thiab dav siv cov khoom noj pheej yig, Qin Jiangfan li al. (1996) nce qhov feem ntawm fiber ntau nplej bran nyob rau hauv cov zaub mov broiler thiab ua thwmsim los ntawm kev ntxiv 0, 0.05%, thiab 0.1% cellulase npaj. Cov txiaj ntsig tau pom tias cov pab pawg ntxiv nrog 0.1% cellulase nce qhov hnyav txhua hnub los ntawm 4.31%, 4.54%, thiab 4.13% feem ntau piv rau pawg tswj hwm ntawm peb theem kev loj hlob ntawm 1-2, 3-6, thiab 7-8, thiab kev noj zaub mov poob los ntawm 1.56%, 4.50%, thiab 4.3% feem.
Xu Qiyou (1998) ntxiv 0.1%, 0.15%, thiab 0.5% cellulase rau cov zaub mov nteg qe. Cov txiaj ntsig tau pom tias thaum lub sij hawm tso qe los ntawm Lub Ib Hlis Ntuj mus rau Lub Kaum Hli, cov qe qe tau nce 0.53%, 1.25%, thiab 2.88%, feem. Cov qe tawg tau txo qis los ntawm 34.49% thiab 16.19% hauv 0.15% thiab 0.5% enzyme theem pawg, thiab cov qe qe lub zog nce los ntawm 14.71% thiab 8.41%, feem.
4. Npua noj
Raws li Yin Qingqiang et al. (1992), ntxiv 0.6% thiab 1.2% cellulose complex enzymes rau cov zaub mov basal ua rau 16.84% thiab 21.86% nce hauv qhov hnyav nce rau cov npua loj hlob thiab fattening piv rau pawg tswj hwm, feem. Wank et al. (1993) qhia tias qhov sib ntxiv ntawmCellulase hmoovnce lub digestibility ntawm nruab nrab detergent fibers ntawm 30.3% mus rau 34.1%, lub digestibility ntawm acidic detergent fibers los ntawm 68.8% mus rau 73.9%, thiab lub digestibility ntawm lub zog ntawm 69.3% mus rau 71.8%.
Ib txoj hauv kev npaj cellulase, uas yog tus cwj pwm hauv nws muaj cov kauj ruam hauv qab no:
Lub slant hom ntawm lub raj kuaj yog Trichoderma viride; Test tube culture medium formula, (NH4) 2SO4 0.2-0.5g, NaNO3 0.1-0.2g, K2HPO40. l-o 15g, MgSO4 · 7H{10}}g, agar 1.0-1.5g, kais dej 99 lOlmL, tag nrho qhov hnyav 102g; Cov khoom xyaw sib tov sib xyaw thiab muab tso rau hauv kab lis kev cai tais diav . 0. IMI ^ a yog sterilized rau 30-35min rau standby; Inoculation thiab kab lis kev cai: hloov ib lub nplhaib ntawm cov hlab ntsha slant cov kab mob mus rau hauv cov dej tsis muaj menyuam nrog lub nplhaib inoculating kom tshem tawm cov kab mob, inoculate rau hauv kab lis kev cai tais diav, muab tso rau hauv lub tank 70-7 nrog qhov kub thiab txias ntawm 30 ° C ± 1 ° C, thiab muab nws tso rau;
Preparation of culture medium, with the formula of 75-85g bran, 15-25g rice bran, (NH4) 2SO4O 25-0. 35g, NaNO3O. 10-0. 15g, MgSO4O. 05-0. 10g, K2HPO4O. 05-0.IOg, water 1480-1520mL, total weight 1600g; Preparation: Mix bran and rice bran evenly, calculate the amount of water, dissolve (NH4) 2S04, NaNO3>MgSO4, K2HPO4 nyob rau hauv dej, sib tov nyob rau hauv cov sib tov ntawm bran thiab mov bran, sib tov zoo, muab tso rau hauv ib tug sterilized triangular hwj, 40-50g ntawm cov ntaub ntub dej yog ntim rau hauv txhua txhua 500mL daim duab peb sab, 0. IMpa yog sterilized rau 30-35min, txias, thiab standby; Inoculation thiab kab lis kev cai;
Cov mis nyuj nruab nrab: bran 70-80g, nplej bran 15-20g, carboxymethyl cellulose 0.15-0.2g, (NH4) 2SO4O 05-0. lg,MgSO4O. 05-0. 10g,K2HPO4O. 1-0.15g, dej 1480-1520mL, tag nrho qhov hnyav 1600g; Kev npaj: thawj zaug yaj carboxymethyl cellulose, (NH4) 2S04, MgSO4, K2HPO4 hauv dej, kho cov pH mus rau 4.5, sib tov nyob rau hauv bran nplej bran sib tov, do tusyees, sterilize ntawm 95-100℃C rau 1h nyob rau hauv ib txwm siab, thiab tuav cov sib tov rau 30-40min; Inoculation thiab kab lis kev cai;
Lub ntim kub yog 30-35 ° C, thickness yog 25-35cm, thiab cov khoom kub yog tswj ntawm 30-37 ° C thoob plaws hauv kab lis kev cai; Raws li qhov kev faib ua feem ntawm cov huab cua hauv tsev thiab huab cua ntshiab hauv tag nrho cov txheej txheem cog qoob loo, qhov txheeb ze av noo hauv chav tswj yog 85% - 95%, thiab lub sijhawm cog qoob loo yog 40 ~ 50h. Tom qab tawm ntawm lub thawv, nws yog crushed thiab qhuav. Cov haujlwm ntawm koji cellulase yog siab dua 6000IU / g qhuav hmoov;
Ua ntej crushed bran koji, thiab tsau nws nyob rau hauv 2.5 lub sij hawm dej ntawm 30-35℃C- ¾ Tom qab ntawd, nias lub lim kom tau cov enzyme tov, thiab ces rov tsau bran koji. Tom qab ntawd, nias lub lim kom tau txais cov tshuaj enzyme. Kho cov pH rau 2.9-3. 1 nrog 10-15% HCl, thiab cia nws sawv ntawm 10-20 ° C rau 0.4-0.6 h. Tom qab ntawd, centrifugate nws. Coj tus supernatant thiab kho pH rau 4.3-4. 5.
Nyob rau tib lub sijhawm, txias cov tshuaj enzyme thiab cawv rau 5-7℃C feem; Ncuav cov enzyme tov rau hauv ethanol thiab do nws tusyees kom lub concentration ntawm enzyme tov ntawm 60-65wt%. Nyob rau hauv lub tank 1.8-2. Tom qab nag lossis daus tiav, centrifugate nws nrog lub centrifuge cais cov nag lossis daus. Tom qab tau txais nag lossis daus, ntxuav nws rau 2-3 zaug nrog 96% ethanol. Qhuav nws ntawm 25-30 ° C thiab tsoo nws kom taucellulase hmoov.
Muaj ob txoj kev tsim khoom tseem ceeb rau cellulase, uas yog cov khoom fermentation thiab kua fermentation, uas yog raws li nram no:
1. Cov yam ntxwv cuam tshuam rau kev tsim cov enzymes thiab kev ua haujlwm
Ntxiv nrog rau cov kab mob kab mob, muaj ntau lwm yam uas cuam tshuam rau kev tsim cov cellulase thiab kev ua haujlwm, suav nrog kab lis kev cai kub, pH tus nqi, av noo, substrate, kab lis kev cai lub sij hawm, thiab lwm yam. Cov yam ntxwv no tsis cais, tab sis cuam tshuam.
Zhang Zhongliang et al. (1997) siv uniformly tsim hom Cl12 (1210) thiab T. ViriclePers. expr los tshawb xyuas cov teebmeem ntawm tsib yam cuam tshuam rau kev tsim cov cellulase ntawm enzyme yield thiab kev ua haujlwm. Lawv ntseeg tias cov ntsiab lus fiber ntau ntawm 40% hauv cov khoom siv substrate, tus nqi pib pH ntawm 7.5, dej ntxiv 4 zaug, thiab kev cog qoob loo ntawm 26-31℃C rau 45 teev tuaj yeem ua tiav qhov siab tshaj plaws ntawm cov enzymes ntawm 26mg / g thiab 20mg / g CMC enzyme kev ua.
h. Wang Chenghua et al. (1997) kuj tau tshawb xyuas cov enzyme ntau lawm rau kev tshuaj ntsuam mutant Trichoderma reesei 91-3. Cov txiaj ntsig tau pom tias cov kab mob tau coj mus rau hauv qhov sib xyaw ntawm 7: 3 ntawm cov nplej straw hmoov thiab nplej bran, nrog rau qhov nruab nrab zoo muaj 4% ammonium sulfate, 0.4% potassium dihydrogen, thiab 0.1% magnesium sulfate. Qhov kev cog qoob loo tsim nyog yog 28-32 ° C, qhov zoo tshaj plaws yog 30 ° C, thiab qhov zoo tshaj plaws inoculation yog 4%. Lub ncov fermentation yog mus txog tom qab 96 teev.
Zhang Linghua et al. (1998) tau kawm txog qhov zoo tshaj plaws fermentation tej yam kev mob rau lub siab -yield cellulase ua strain Wu-932 tau los ntawm mutagenesis, uas siv Aspergillus niger W-925 ua hom pib. Cov txiaj ntsig tau pom tias qhov zoo tshaj plaws fermentation tej yam kev mob yog 1: 2 nplej bran thiab mov straw hmoov raws li kab lis kev cai nruab nrab, 5% inoculation tus nqi, nruab nrab nplej straw crushing ntev ntawm 3-5 millimeters, pib pH nqi ntawm 4-5, kub ntawm 28-35 ° C, thiab fermentation lub sij hawm ntawm 72 teev.
2. Tswj cov kab mob sib kis
Muaj cov kab mob sib kis ntawm pub cellulases, feem ntau hu ua "cov kab mob plaub hau dawb". Tom qab muaj kuab paug, kev ua haujlwm enzyme txo qis hauv cov mob me me thiab fermentation tsis ua rau mob hnyav. Yog li ntawd, kev kawm txog kev tswj cov pa phem fermentation yog qhov tseem ceeb heev. Zhang Linghua et al. (1998) tau kawm txog cov yam ntxwv colony, qhov chaw, kev loj hlob thiab lub cev ntaj ntsug, nrog rau kev tswj hwm ntawm "dawb plaub hau fungus", thiab pom ib qho chaw sov Candida J-931 uas yog nyob rau hauv kev sib raug zoo nrog Trichoderma harzianum Wu-932 thiab hauv kev sib tw inhibitory kev sib raug zoo nrog "dawb plaub hau fungus". Siv cov kab mob no rau kev sib xyaw fermentation tuaj yeem tswj tau cov kab mob "dawb plaub hau dawb".
kev faib tawm
1. Los ntawm kev muaj pes tsawg leeg thiab kev ua haujlwm
Cellulases tuaj yeem muab faib ua endo-1,4- - D-glucan hydrolase lossis endo-1,4- - D-glucanase (EC 3.2.1.4), EG los ntawm fungi, Cen los ntawm cov kab mob, exo-1, 4- - D-glucanase (EC. 3.9. cov kab mob, thiab BG raws li lawv cov tshuaj tiv thaiv catalytic. Endo-1,4-glucanase random cleaves amorphous cheeb tsam hauv cellulose polysaccharide chains. Tsim cov oligosaccharides ntawm qhov ntev sib txawv thiab cov saw tshiab xaus. Exoglucanase ua rau qhov kawg ntawm cov kev txo qis thiab tsis txo cov cellulose polysaccharide chains, tso cov piam thaj los yog cellobiose . - glucosidase hydrolyzes cellulose disaccharides los tsim ob lub suab ntawm cov piam thaj. Fungal cellulases muaj ntau lawm thiab kev ua haujlwm, thiab feem ntau yog siv hauv kev yug tsiaj thiab pub khoom noj los ntawm cov kab mob fungal.
2. Raws li cov txheej txheem degradation
Qhov sib txawv tseem ceeb ntawm cov tshuaj tiv thaiv cellulase thiab cov tshuaj tiv thaiv enzyme dav dav yog tias cellulase yog ntau -cov khoom siv enzyme thiab cov qauv substrate yog qhov nyuaj heev. Vim lub insolubility ntawm lub substrate, lub adsorption ntawm cellulase hloov cov txheej txheem ntawm tsim ES complexes ntawm lub enzyme thiab lub substrate. Cellulase thawj zaug tshwj xeeb adsorbs mus rau substrate cellulose, thiab tom qab ntawd decomposes cellulose rau hauv qabzib los ntawm kev sib koom ua ke ntawm ntau yam.
Xyoo 1950, Reese et al. tau npaj lub tswv yim C1 Cx, uas qhia tias sib txawv enzymes yuav tsum ua hauj lwm ua ke kom huv si hydrolyze cellulose rau hauv qabzib. Cov nyhuv synergistic feem ntau ntseeg tau tias C1 enzyme ua ntej tawm tsam thaj tsam amorphous ntawm cellulose, tsim ib qho tshiab dawb kawg uas yuav tsum tau muaj rau Cx, thiab tom qab ntawd CX enzyme cleaves fibrous disaccharide unit los ntawm kev txo lossis tsis txo qhov kawg ntawm cov saw hlau polysaccharide. Thaum kawg, - glucosidase hydrolyzes fibrous disaccharide rau hauv ob chav piam thaj. Txawm li cas los xij, kev sib koom ua ke ntawm cellulase tsis yog qhov tseeb, thiab cov kev tshawb fawb tom ntej tau pom tias C1 Cx thiab - glucosidase yuav tsum sib koom ua ke kom hydrolyze natural cellulose. Yog tias C1 enzyme yog siv los ua crystallize cellulose ua ntej, ces C1 enzyme raug tshem tawm, thiab Cx enzyme ntxiv, qhov kev ua tiav no tsis tuaj yeem hydrolyze crystalline.Cellulase hmoov.
FAQ
Cellulase ua dab tsi rau lub cev?
Yog li, cellulase enzymes muaj txiaj ntsig zoo rau tib neeg lub cev. Ua ntej ntawm tag nrho cov, lawv tswj tswj cov ntshav qab zib theem, los ntawm kev hloov cellulose mus rau beta-glucose. Tsis tas li ntawd, lawv khaws cov roj cholesterol kom zoo thiab tuaj yeem txo cov roj cholesterol.
Puas yog tib neeg xav tau cellulase?
Tib neeg tsis muaj cellulase, enzyme xav tau los rhuav tshem cellulose. Qhov no yog vim li cas tib neeg tsis tuaj yeem zom cov cellulose. Cellulose yog ib yam khoom nyuaj uas xav tau cov enzyme cellulose kom degradation kom raug.
Dab tsi yog cellulase enzyme siv rau?
Hauv kev sib koom ua ke nrog lwm cov enzymes xws li pectinases thiab hemicellulases, cellulases cuam tshuam cov txheej txheem kev ncaj ncees ntawm cov nroj tsuag cell phab ntsa thiab txhim kho cov extraction ntawm lub hom phiaj molecules. Yog li ntawd, lawv yog siv nyob rau hauv txhua txoj kev uas muaj cov txheej txheem ntawm cov nroj tsuag raws li cov ntaub ntawv.
Dab tsi yog cellulase hauv zaub mov?
Qhov teeb meem loj nrog cov zaub mov cog yog cov phab ntsa ntawm tes, ib qho kev sib txuas uas nyob ib puag ncig cov nroj tsuag. Ib feem tseem ceeb ntawm cov phab ntsa ntawm tes yog qee yam hu ua cellulose. Cellulase yog ib qho enzyme uas yog ib feem tseem ceeb ntawm kev zom cov nroj tsuag fiber ntau, tab sis nws tsis yog ib yam dab tsi uas peb ib txwm tsim hauv peb lub cev.
Cim npe nrov: Cellulase hmoov CAS 9012-54-8, lwm tus neeg, manufacturers, hoobkas, lag luam wholesale, yuav, nqe, tej, kev muag khoom