7-Nitroindole hmoovyog cov organic compound nrog cov tshuaj formula C8H6N2O2 thiab CAS naj npawb 6960-42-5. Nws yog xim daj rau txiv kab ntxwv crystalline hmoov nrog ib qho tsw ntxhiab tsw. Nws yog dav siv nyob rau hauv kev tshawb fawb nyob rau hauv lub teb ntawm chemistry, pharmacology, thiab biology, xws li raws li ib tug fungicide, antioxidant, thiab rau nws cov dej num hauv DNA replication thiab kho. Hauv kev kho mob, nws tseem muaj cov tshuaj tiv thaiv kab mob qog noj ntshav thiab cov teebmeem neuroprotective. Nws yog ib qho tseem ceeb organic compound uas ua lub luag haujlwm tseem ceeb hauv kev tshawb fawb thiab kev siv. Nws yog ib qho tseem ceeb raw khoom rau synthesizing tej yam tshuaj. Los ntawm cov tshuaj tiv thaiv kab mob, nws tuaj yeem hloov mus rau hauv cov tshuaj sib xyaw nrog cov tshuaj pharmacological, uas tuaj yeem siv los kho ntau yam kab mob.
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Tshuaj Formula |
C8H6N2O2 |
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Exact Mass |
162 |
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Molecular Luj |
162 |
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m/z |
162 (100.0%), 163 (8.7%) |
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Elemental Analysis |
C, 59.26; H, 3.73; N, 17.28; O, 19.73 |
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Cov hauv qab no yog cov kev siv ntawm7-Nitroindole hmoov:
1. Kawm txog cov kab mob neurodegenerative:
Nws tuaj yeem siv los ua ib qho ntawm cov cuab yeej sib txuas rau kev kawm cov kab mob neurodegenerative. Raws li kev tshawb fawb, nws muaj kev tiv thaiv zoo rau cov neurons hauv lub cev, thiab tuaj yeem txo qhov℃ntawm DNA oxidation, lipid peroxidation thiab apoptosis, yog li tiv thaiv kev tuag ntawm paj hlwb. Tsis tas li ntawd, nws tuaj yeem siv los ua cov cuab yeej siv los ua kom -7 nicotinic acid acetylcholine receptor (nAChR) hauv cov paj hlwb, thiab tiv thaiv kev tuag ntawm cov neuron los ntawm cov lus teb ntawm nAChR thiab cov cim qhia txog kev tswj hwm lub cev.
2. Kawm txog tus cwj pwm neuronal:
Vim nws muaj kev tiv thaiv los ntawm neurons, nws kuj yog siv los ua ib qho cuab yeej los kawm txog kev ua haujlwm neuronal. Cov kws tshawb fawb tuaj yeem siv nws los txheeb xyuas cov fluorescence ntawm cov neurons, thiab tom qab ntawd kawm txog cov haujlwm ntawm cov neurons, kev txav ntawm lub davhlau ya nyob twg thiab lub xeev cov lus teb. Nyob rau tib lub sijhawm, txoj haujlwm no tseem tuaj yeem pab cov kws tshawb fawb neuroscientists zoo nkag siab txog qhov xwm txheej ntawm cov neurons nyob rau hauv ib txwm muaj thiab pathological mob, thiab tshawb nrhiav qhov muaj peev xwm kho cov kab mob neurodegenerative yav tom ntej.
3. Kawm txog cov noob caj noob ces:
Nws muaj cov nyhuv chromosome staining thiab tuaj yeem ua ke nrog DNA los ua ib qho nyuaj. Hauv kev kawm txog cov noob caj noob ces, cov kws tshawb fawb tuaj yeem siv cov khoom lag luam ua cov fluorescent stain kom pom qhov kev faib tawm ntawm DNA molecules uas nthuav tawm kev txaus siab, xws li hloov cov noob caj noob ces, hauv cov nucleus. Cov khoom saum toj no - tau hais txog nws ua rau nws muaj ntau yam kev siv hauv biology, xws li siv nws rau kev tshuaj ntsuam genetic diagnostic test rau tej yam kab mob, thiab saib xyuas cov kev qhia ntawm cov noob nyob rau hauv txawv tej yam kev mob los ntawm kev saib xyuas ntawm dyes.
4. Siv ua tshuaj tua kab thiab fungicide:
Zoo li lwm cov tshuaj nitro, cov pab pawg nitro ntawm nws tau yooj yim txo mus rau nitroso, thiab tom qab ntawd cuam tshuam nrog ntau yam enzymes ntawm cov kab mob, yog li rhuav tshem nws cov phab ntsa ntawm tes. Yog li ntawd, nws ua raws li ib tug fungicide. Tsis tas li ntawd, nws kuj tseem siv rau hauv kev ua liaj ua teb ua tshuaj tua kab los pab cov qoob loo ua liaj ua teb los ntawm kab puas tsuaj.
Hauv kev xaus, nws muaj ntau yam kev siv hauv ntau qhov chaw. Los ntawm cov qauv uas tuaj yeem simulate cov kab mob neurodegenerative los pab cov kws tshawb fawb neuroscientists zoo nkag siab txog kev ua haujlwm neuronal, gene qhia, thiab tshuaj tua kab thiab fungicides, nws yog ib qho ntawm cov khoom siv cuam tshuam.
7-Nitroindole hmoov,Raws li cov organic compound, muaj cov pab pawg ua haujlwm tshwj xeeb nitro thiab indole rings hauv nws cov qauv tshuaj, uas ua rau nws muaj qee yam kev siv hauv kev siv tshuaj tua kab, tshwj xeeb tshaj yog cov tshuaj tua kab. Cov txheej txheem ntawm kev ua ntawm cov derivatives no tuaj yeem koom nrog ntau yam, suav nrog kev cuam tshuam nrog cov kab mob paj hlwb, cuam tshuam lawv cov txheej txheem physiological thiab metabolic, thiab inhibiting lawv txoj kev loj hlob thiab kev loj hlob.
Lub paj hlwb ntawm kab yog ib qho tseem ceeb ntawm kev tswj hwm qhov chaw rau lawv lub neej kev ua ub no. Ntau cov tshuaj tua kab ua tiav lawv cov hom phiaj tshuaj tua kab los ntawm kev cuam tshuam nrog kev ua haujlwm ntawm cov kab paj hlwb. Qee qhov derivatives ntawm 7-nitroindle yuav muaj cov txheej txheem zoo sib xws. Lawv tuaj yeem simulate lossis cuam tshuam nrog cov txheej txheem sib kis ntawm cov kab mob neurotransmitters, xws li acetylcholine thiab gamma aminobutyric acid (GABA), ua rau muaj kev ua haujlwm tsis zoo ntawm kab paj hlwb, ua rau cov tsos mob xws li tuag tes tuag taw, tuag tes tuag taw, thiab txawm tuag.
Piv txwv li, qee lub tebchaw uas muaj nitro functional pawg tuaj yeem cuam tshuam cov haujlwm ntawm acetylcholinesterase hauv cov kab, ua rau cov acetylcholine tsub zuj zuj hauv synaptic cleft, nruam stimulation ntawm neural receptors, thiab ua kom kab nyob rau hauv lub xeev ntawm sustained excitation, thaum kawg ua rau tuag vim paj fatigue. Cov txheej txheem ntawm kev ua haujlwm no zoo ib yam li cov tshuaj tua kab organophosphate thiab carbamate insecticides.
Cov txheej txheem physiological metabolic ntawm kab yog lub hauv paus ntawm lawv lub neej kev ua ub no. Cov derivatives ntawm 7-nitroindle tuaj yeem tsim cov tshuaj tua kab mob los ntawm kev cuam tshuam cov kab hluav taws xob metabolism, cov tshuaj metabolism, lossis cov metabolism hauv detoxification. Piv txwv li, qee cov derivatives tuaj yeem cuam tshuam kev ua haujlwm ntawm cov kab mob ua pa hauv cov kab mob, thaiv ATP synthesis, thiab ua rau tsis muaj zog txaus thiab tua kab. Los yog lawv tuaj yeem cuam tshuam nrog cov lipid metabolism, protein metabolism, thiab lwm yam txheej txheem ntawm kab, ua rau stunted kev loj hlob thiab kev loj hlob los yog kev tuag.
Tsis tas li ntawd, qee cov derivatives kuj tseem tuaj yeem cuam tshuam cov kev ua ntawm kab detoxifying enzymes. Muaj ntau yam detoxifying enzymes nyob rau hauv kab, xws li cytochrome P450 enzymes thiab glutathione S-transferase, uas muaj peev xwm catalyze cov metabolism thiab excretion ntawm txawv teb chaws tshuaj lom. Yog hais tias derivatives ntawm 7-nitroindle tuaj yeem cuam tshuam cov kev ua ntawm cov enzymes detoxifying, kab 'lub peev xwm los detoxify cov tshuaj lom yuav txo qis, ua rau lawv raug tshuaj tua kab ntau dua.
Kev loj hlob thiab kev loj hlob ntawm kab yog ib txoj kev nyuaj uas muaj kev tswj hwm ntawm ntau cov noob thiab cov kev taw qhia. Qee qhov derivatives ntawm 7-nitroindle tuaj yeem cuam tshuam cov kab kev loj hlob thiab kev loj hlob los ntawm kev cuam tshuam nrog cov haujlwm ib txwm muaj ntawm cov noob no thiab cov kev taw qhia. Piv txwv li, lawv tuaj yeem cuam tshuam cov synthesis lossis qhia txoj hauv kev ntawm cov tshuaj hormones molting hauv kab, ua rau lawv tsis tuaj yeem molt thiab loj hlob ib txwm. Los yog lawv tuaj yeem cuam tshuam rau cov menyuam yaus cov tshuaj hormones qhia txoj hauv kev ntawm cov kab, ua rau lawv nyob hauv lub xeev larval thiab tsis tuaj yeem nkag mus rau cov neeg laus.
Tsis tas li ntawd, qee qhov derivatives kuj tseem tuaj yeem muaj peev xwm tiv thaiv kab tua kab. Lawv tuaj yeem cuam tshuam nrog kev loj hlob ntawm cov kab me me, kev coj tus cwj pwm mating, lossis cov txheej txheem qe nteg, yog li txo qhov kev loj hlob ntawm kab. Qhov no yog qhov tseem ceeb heev rau kev tswj cov kab tsuag thiab txo kev siv tshuaj tua kab.
Tshuaj ntsuam xyuas ntawm7-Nitroindole hmoovyuav tsum tau siv ntau txoj hauv kev, suav nrog kev ntsuas purity, tus yam ntxwv ntawm tus yam ntxwv, thiab kev ntsuas kev ruaj ntseg, thiab lwm yam. Cov hauv qab no muab kev tshuaj xyuas los ntawm ntau qhov kev xav:
Purity Detection Methods
Gas Chromatography (GC)
Using a gas chromatograph to separate the components in the mixture and employing a detector (such as a FID flame ionization detector) to quantitatively analyze the purity of 7-Nitroindole. This method is suitable for volatile samples and can detect trace impurities. For example, the purity of the product provided by a certain supplier is labeled as >98.0% (GC), qhia tias nws purity tau raug txheeb xyuas los ntawm cov roj chromatography.
High Performance Liquid Chromatography (HPLC)
Rau cov tebchaw uas tsis ruaj khov ntawm qhov kub thiab txias lossis muaj zog polarity, HPLC yog qhov kev xaiv zoo dua. Kev sib cais yog ua tiav los ntawm kev thim rov qab- theem kem (xws li C18 kem), ua ke nrog lub ntsuas hluav taws xob ultraviolet (UV) lossis diode array detector (DAD), ua kom muaj siab - kuaj pom. Piv txwv li, lub purity ntawm 7-Nitroindole-2-carboxylic acid (ib tug derivative) tau txheeb xyuas los ntawm HPLC kom ntau dua los yog sib npaug rau 98%.
Nuclear Sib Nqus Resonance Hydrogen Spectroscopy (1H NMR)
Los ntawm kev txheeb xyuas cov tshuaj hloov pauv, thaj chaw ncov, thiab kev sib txuas tsis tu ncua ntawm hydrogen atoms, cov qauv molecular tuaj yeem lees paub thiab ntsuas purity. Piv txwv li, nyob rau hauv 1H NMR spectrum ntawm 7-Nitroindole, cov cim qhia ntawm aromatic hydrogens (xws li δ 8.16, 7.46 ppm) thiab kev hloov pauv los ntawm cov nyhuv electron ntawm pawg nitro yog cov pov thawj tseem ceeb rau kev pom zoo ntawm cov qauv.
Cov Txheej Txheem Txheej Txheem Txheej Txheem
Mass Spectrometry (MS)
Electrospray Ionization Mass Spectrometry (ESI-}MS) lossis Electron Impact Mass Spectrometry (EI-MS) tuaj yeem txiav txim siab txog cov molecular ion ncov thiab fragment peaks, txheeb xyuas qhov hnyav molecular thiab cov khoom tawg. Piv txwv li, lub ncov molecular ion ntawm 7-Nitroindole (m / z 162.15) zoo ib yam nrog nws qhov hnyav molecular, txhawb nws cov tshuaj formula C8H6N2O2.
Infrared Spectroscopy (IR)
Los ntawm kev txheeb xyuas cov yam ntxwv ntawm qhov siab tshaj plaws, qhov pom ntawm cov pab pawg ua haujlwm tuaj yeem paub tseeb. Piv txwv li, lub zog nqus peaks ntawm cov pab pawg nitro (1500-1600 cm⁻¹ thiab 1300-1400 cm⁻¹) thiab C=C stretching vibration ntawm lub nplhaib indole (1600-1650 cm⁻¹) yog cov pov thawj tseem ceeb rau kev pom zoo ntawm cov qauv ntawm 7.Noleind.
X-Ray Ib Crystal Diffraction (XRD)
Rau ib qho qauv siv lead ua, XRD tuaj yeem txiav txim siab qhov kev teeb tsa molecular spatial thiab daim ntawv cog lus ntev, lub kaum sab xis, thiab lwm yam. Piv txwv li, hauv kev tshawb fawb, XRD tau siv los tshuaj xyuas cov qauv ntawm cov receptor uas muaj 7-Nitroindole pab pawg thiab bis-dihydropyrophosphate ion complex, nthuav tawm nws cov kev sib cuam tshuam supramolecular.
Txoj kev ntsuas kev ruaj ntseg
Thermogravimetric Analysis (TGA)
Nyob rau hauv qhov programmed kub nce, TGA tuaj yeem saib xyuas qhov kev hloov pauv hauv cov qauv loj nrog qhov kub thiab txias, ntsuas thermal stability. Piv txwv li, lub melting point ntawm 7-Nitroindole yog 94-98 degree. TGA tuaj yeem txiav txim siab ntxiv nws qhov kub thiab txias, coj kev teeb tsa ntawm kev cia khoom.
High Performance Liquid Chromatography Stability Test
Cov qauv raug muab tso rau hauv qhov sib txawv ntawm qhov kub thiab txias (xws li 4 degree, 25 degree, 40 degree) lossis raug rau lub teeb, thiab cov qauv coj mus kuaj tsis tu ncua rau HPLC purity test. Piv txwv li, ib txoj kev tshawb fawb tau pom tias 7-Nitroindole tuaj yeem ruaj khov rau 2 xyoos ntawm -20 degree, tab sis tsuas yog rau 1 xyoos ntawm 25 degree.
Solubility Test
Kev solubility ntawm cov qauv hauv cov kuab tshuaj sib txawv (xws li DMSO, dej, cawv) yog txiav txim siab los muab cov ntaub ntawv siv rau kev sim tom ntej. Piv txwv li, qhov solubility ntawm 7-Nitroindole hauv DMSO yog 100 mg / mL (616.71 mM), thiab DMSO tshiab yuav tsum tau siv kom tsis txhob muaj kev nqus dej noo.
Daim ntawv thov -txoj kev tsom xam tsom xam
Kev tshawb fawb ntawm Photochemical Precursor
Lub 7-Nitroindole nucleoside tuaj yeem siv los ua cov txheej txheem photochemical los tsim 2'-deoxyribose sab hauv cleavage puas qhov chaw hauv DNA chains. Cov kev hloov pauv ntawm qhov nqus spectrum tom qab teeb pom kev tuaj yeem raug saib xyuas los ntawm ultraviolet - pom spectroscopy (UV-Vis) los txheeb xyuas cov kev ua haujlwm photolytic.
Kev tshuaj xyuas bioactivity
Hauv kev tsim tshuaj, kev ua haujlwm lom neeg ntawm 7-Nitroindole thiab nws cov derivatives yuav tsum tau soj ntsuam. Piv txwv li, cov tshuaj muaj peev xwm kho tau nrog tus nqi tuaj yeem kuaj xyuas los ntawm kev kuaj enzyme inhibition (xws li APE1 nucleases inhibition test) lossis cytotoxicity tests (xws li MTT method).
FAQ
1. 7-nitroindole yog dab tsi?
Nws yog ib qho organic compound ntawm cov chav kawm indole, nrog cov qauv uas 7 txoj hauj lwm ntawm lub nplhaib indole hloov los ntawm ib pab pawg nitro (-NO₂). Nws feem ntau yog siv los ua ib qho precursor rau fluorescent probes los yog fluorescent marker hauv kev tshawb fawb biochemical.
2. Cov ntawv thov tseem ceeb yog dab tsi?
Nws yog tsuas yog siv los ua cov khoom siv raw rau kev sib txuas ntawm fluorescent probes, tshwj xeeb tshaj yog rau cov cim nucleotides hauv DNA / RNA sequencing; nws kuj tseem siv tau rau hauv kev tshawb fawb ntawm cov protein-nucleic acid kev sib cuam tshuam, nrog rau hauv kev tshawb fawb ntawm optical physics / optical chemistry.
3. Dab tsi yuav tsum tau sau tseg thaum siv nws?
Nws yuav tsum tau muab khaws cia rau hauv qhov chaw tsaus thiab txias vim tias nws nkag siab rau lub teeb; kev khiav hauj lwm yuav tsum tau nqa tawm nyob rau hauv lub fume hood kom tsis txhob nqus tau los yog tawv nqaij; cov pov tseg yuav tsum raug kho raws li cov tshuaj phem.
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