Shaanxi BLOOM Tech Co., Ltd. yog ib tug ntawm cov uas paub txog manufacturers thiab lwm tus neeg ntawm rhodamine 110 cas 13558-31-1 nyob Suav teb. Txais tos rau cov lag luam wholesale bulk zoo zoo rhodamine 110 cas 13558-31-1 muag ntawm no los ntawm peb lub hoobkas. Kev pabcuam zoo thiab tus nqi tsim nyog muaj.
Rhodamine 110yog ib qho zoo heev oxazine-raws li ntsuab fluorescent zas xim. Endowed nrog cov khoom tseem ceeb xws li siab photostability, siab quantum yield, pH insensitivity (ruaj khov fluorescence nyob rau hauv lub pH ntau ntawm 4-10), thiab zoo biocompatibility, nws tau dhau los ua ib tug nquag siv fluorescent cuab tam nyob rau hauv ntau lub teb. uas ua rau nws siab tshaj 488 nm lasers thiab txheem FITC lim, yog li boasting broad applicability. Cov pab pawg ua haujlwm nquag xws li pawg carboxyl thiab NHS esters tuaj yeem nkag mus rau hauv cov zas xim los ntawm kev hloov kho cov qauv kom hloov kho nws cov kev ua tau zoo, ua tiav ob qho tib si siab thiab kev ua tau zoo.
|
Tshuaj Formula |
C20H15ClN2O3 |
|
Exact Mass |
366 |
|
Molecular Luj |
367 |
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m/z |
366 (100.0%), 368 (32.0%), 367 (21.6%), 369 (6.9%), 368 (2.2%) |
|
Elemental Analysis |
C, 65,49; H, 4.12; lwj, 9,66; nqe 7,64;. O, 13.09 Nws |
Daim ntawv thov
Cellular thiab Hauv Vivo Imaging
Vim nws siab photostability thiab pH insensitivity, nws thiab nws cov derivatives tau siv dav hauv kev ntsuas cellular thiab hauv vivo taug qab. Nyob rau hauv cellular imaging, lawv tuaj yeem siv rau kev soj ntsuam fluorescence microscopy thiab confocal microscopy imaging ntawm ob leeg nyob thiab taag hlwb, qhia meej meej lub subcellular localization, faib, thiab dynamic hloov ntawm lub hom phiaj molecules. Piv txwv suav nrog mitochondria- phiaj xwm duab (nws daim ntawv cationic tuaj yeem sau hauv mitochondria) thiab kev pom ntawm cov teeb liab ntawm tes hloov txoj hauv kev.
Nyob rau hauv vivo imaging, tom qab tus Tsov tus tw vein txhaj lossis kev tswj hwm hauv zos,Rhodamine 110tuaj yeem siv tau tiag tiag-lub sijhawm taug qab cov kev faib tawm thiab cov txheej txheem metabolic ntawm cov ntawv sau npe hauv cov kab mob xws li nas thiab zebrafish embryos. DBCO- hloov kho derivatives tuaj yeem ua tiav hauv vivo labeling nyob rau hauv feeb, muab lub sijhawm tiag tiag - cov cuab yeej pom lub sijhawm rau kev tshawb fawb biomedical. Lub caij no, nws cov teeb liab fluorescent ruaj khov yog resistant rau photobleaching, uas tuaj yeem ua tau raws li cov kev xav tau ntawm kev yees duab ntev -.
Kev txheeb xyuas cov pov thawj lom neeg
Hauv kev tshawb fawb forensic, nws tuaj yeem siv rau kev tshawb nrhiav thiab kev txheeb xyuas cov kua roj ntsha lom, hais txog cov kev txwv ntawm kev tshawb pom ib txwm muaj xws li tsis zoo thiab kev puas tsuaj DNA. Los ntawm kev sib xyaw Rhodamine 110-raws li cov fluorescent peptide substrates, cov kab hauv zos tuaj yeem ua tiav los ntawm cov enzymatic hydrolysis ntawm lub cev cov kua dej tshwj xeeb proteases. Tom qab txau cov substrate rau thaj chaw uas tsis txaus ntseeg, yog tias muaj cov kua dej hauv lub cev xws li ntshav, phev, lossis qaub ncaug, cov proteases tshwj xeeb hauv lawv yuav tshem tawm cov substrate kom tso tawm fluorescence.
Lub teeb liab fluorescent ua rau muaj qhov tseeb hauv cheeb tsam ntawm cov kua roj ntsha lom yam tsis muaj kev cuam tshuam nrog kev rho tawm DNA tom qab thiab ntaus ntawv.
Txoj kev tshawb pom no tsim nyog rau kev zais, taug qab, lossis keeb kwm yav dhau los -cov kua roj ntsha tsaus. Piv nrog rau cov txheej txheem ib txwm muaj xws li ultraviolet teeb irradiation thiab colorimetry, nws muaj qhov zoo ntawm qhov muaj zog tshwj xeeb, siab rhiab heev, thiab kev puas tsuaj tsawg heev rau cov qauv kuaj. Nws tuaj yeem txhim kho qhov ua tau zoo thiab qhov tseeb ntawm kev tshawb xyuas cov pov thawj lom neeg lom neeg, muab cov ntsiab lus tseem ceeb rau kev tshawb nrhiav kev ua phem.
Kev nthuav dav hauv cov ntaub ntawv thiab cov khoom siv kho qhov muag
Dhau ntawm biomedical teb,Rhodamine 110kuj tuaj yeem siv rau hauv cov xwm txheej xws li kev hloov kho cov khoom siv thiab cov khoom siv kho qhov muag. Hauv cov ntaub ntawv tshawb fawb, nws tuaj yeem raug qhia ua fluorescent sojntsuam rau hauv cov ntaub ntawv xws li hlau -organic moj khaum (MOFs) thiab mesoporous silica los npaj cov khoom siv fluorescent, uas yog siv rau hauv kev saib xyuas ib puag ncig thiab pom cov neeg xa tshuaj. Nws qhov kev hloov kho tso cai rau kev sib txuas nrog PEG derivatives thiab thaiv cov copolymer, optimizing biocompatibility thiab lub hom phiaj muaj peev xwm ntawm cov ntaub ntawv.
Hauv thaj chaw kho qhov muag, nws tuaj yeem ua lub laser dye rau kev tsim cov dye lasers. Tsis tas li ntawd, nws cov khoom fluorescent tuaj yeem siv rau kev tshawb fawb thiab kev tsim kho cov khoom siv kho qhov muag xws li cov xim lim dej, cov kua siv lead ua kua (LCDs), thiab lub teeb -emitting diodes (LEDs). Nws kuj tseem tuaj yeem siv los ua kom zoo dua qhov kev ua tau zoo ntawm cov ntaub ntawv zoo li lub hnub ci hlwb thiab kho qhov muag waveguide, txhim kho lub luminous efficiency thiab ruaj khov ntawm cov khoom siv los ntawm kev tsim txiaj ntawm nws cov khoom kho qhov muag ruaj khov.
Tooj liab yog ib qho tseem ceeb kab nyob rau hauv tib neeg lub cev. Kev poob ntawm tooj liab hauv lub cev yuav ua rau muaj kev cuam tshuam ntawm metabolic thiab ntau yam kab mob, xws li cov roj (cholesterol) ntau ntxiv, txo qis cov hlab ntsha elasticity, thiab nce ntshav siab. Kev tshawb fawb ntawm tooj liab ion bioluminescence sojntsuam tau yog lub ntsiab lus kub. Zhao et al. tsim thiab tsim ib qho tshiab rhodamine lactam derivative 5 nyob rau hauv 2009, thiab siv nws mus nrhiav kom tau Cu2+nyob rau hauv aqueous daws thiab nyob hlwb.
Cov tshuaj tiv thaiv ntawm no colorimetric sojntsuam rau tooj liab ion yog instantaneous thiab thim rov qab, thiab nws yuav tsis cuam tshuam nrog colorimetric thiab fluorescence teeb liab ntawm tooj liab ion thaum lub concentration ntawm lwm yam hlau ions siab heev. Cov yam ntxwv no ua rau nws ua tau raws li qhov tshwj xeeb ntawm biomedicine thiab kev saib xyuas ib puag ncig. Tam sim no, cov kev sojntsuam no tau siv dav los kuaj xyuas qhov concentration ntawm tooj liab ions hauv ib puag ncig lub cev thiab ntsuas kev faib cov tooj liab ions hauv cov hlwb nyob. Lawv qhov kev ua tau zoo tshaj plaws qhia tau hais tias qhov kev thov zoo tshaj plaws.
Hlau ion sojntsuam:
Hlau yog ib qho tseem ceeb kab nyob rau hauv tib neeg lub cev. Nws feem ntau muaj nyob rau hauv daim ntawv o
f complex ions nyob rau hauv tib neeg lub cev, tsim hemoglobin thiab myoglobin nrog heme, protein, thiab lwm yam, thiab plays lub luag hauj lwm nyob rau hauv thauj thiab khaws cia oxygen nyob rau hauv lub cev. Vim yog paramagnetism ntawm Fe 3+, Fe3+fluorescence probes yog hom fluorescence quenching, uas tsis haum rau fluorescence imaging thiab nyob rau hauv situ nrhiav tau ntawm Fe3+ nyob rau hauv cov khoom bovine.
Yog li ntawd, kev tsim cov fluorescence txhim kho Fe3+fluorescence sojntsuam raws li lub voj kaw kom qhib lub voj hloov pauv ntawm nws cov molecules tau maj mam nyiam.
Mercury yog cov hlau uas muaj tshuaj lom heev. Cov ntsiab lus mercury thiab mercury ions tuaj yeem nkag mus rau ib puag ncig los ntawm ntau txoj hauv kev. Tom qab lub sijhawm ntev - kis thiab noj, tib neeg lub cev yuav muaj xeev siab, ntuav, mob plab, mob raum thiab lwm yam kab mob, uas ua rau muaj kev phom sij heev. Raws li tib neeg muab qhov tseem ceeb heev rau kev toxicity ntawm mercury, kev tshawb fawb ntawm Hg2+fluorescent probes tau nce ntau xyoo dhau los. Xu et al. tsim thiab tsim ib lub rhodamine thiohydrazide rau ultraviolet thiab fluorescence nrhiav tau Hg2+ nyob rau hauv lub aqueous theem.
Qhov sib piv stoichiometric ntawm qhov kev sojntsuam rau Hg2+ yog 2 ∶ 1. Qian li al. tsim thiab tsim ib qho kev xaiv rhodamine Hg ntau heev2+ fluorescence sojntsuam, uas tuaj yeem tsis tsuas yog paub txog kev kuaj pom fluorescence ntawm Hg2+, tab sis kuj txiav txim siab ua ntej qhov muaj Hg2+ siv cov tshuaj tiv thaiv xim. Kev sojntsuam yog thim rov qab. Thaum EDTA ntxiv rau qhov sib npaug ntawm cov xim kev loj hlob, cov ntshav liab ntawm lub cev yuav tsis muaj xim. Tsis tas li ntawd, cov lus teb ceev fluorescence yog lwm yam ntawm qhov kev sojntsuam no. Tom qab ntxiv Hg2+, ruaj khov thiab muaj zog fluorescence raug tsim tawm tam sim ntawd. Piv nrog cov kev sojntsuam zoo sib xws uas yuav tsum tau muaj sijhawm sib npaug, qhov kev sojntsuam no zoo dua rau qhov tiag -lub sijhawm tsom xam ntawm ib puag ncig lossis cov qauv lom neeg.
Cov txheej txheem staining
(1) Dissolve 0.4mgRh123 hauv 1mL DMSO los npaj 1mMRh123-DMSO tov.
(2) Npaj cov hlwb nrog cov swb. Tus naj npawb ntawm cov hlwb yuav tsum yog 5 × 104 ~ 5 × 105 pieces / mL.
(3) Muab cov swb thiab ntxuav cov hlwb nrog PBS lossis Hank cov tshuaj.
(4) Dilute 1mM Rh123 tov nrog kab lis kev cai nruab nrab los npaj 1 ~ 20µ M Rh123 tsis.
(5) Ntxiv Rh123 buffer tov rau cov swb thiab incubate ntawm 37℃rau 30 feeb mus rau 1 teev.
(6) Tshem tawm Rh123 tsis thiab ntxuav cov hlwb nrog kab lis kev cai nruab nrab (tom qab ntxuav lub hlwb, ntxiv 10% formalin tsis thiab incubate rau 15-20 feeb, ces ntxuav nrog PBS rau fixation).
(7) Cov hlwb raug soj ntsuam nrog lub fluorescent microscope nrog lub lim dej fluorescein.
Cia tej yam kev mob: Khaws nyob rau hauv chav tsev kub thiab deb ntawm lub teeb, yaj nyob rau hauv DMSO los npaj niam cawv, thiab ces cia ntawm - 20℃.
Cov kev mob tshwm sim ntawm cov tshuaj no yog dab tsi?
Cytotoxicity
Concentration dependence: Ntawm cov concentrations qis (qis dua 10 μ M), tsis muaj cytotoxicity tau pom tawm tsam tib neeg lymphoblasts. Thaum cov concentration siab tshaj 100 μ M, cov tshuaj yuav ua rau cov phooj ywg leukemia tuag.
Cell Accumulation: Nws accumulates nyob rau hauv tib neeg lymphoblasts thiab phooj ywg leukemia hlwb, thiab tej zaum yuav hloov pH nqi nyob rau hauv lub hlwb, tshwj xeeb tshaj yog nyob rau hauv daim ntawv ntawm cations accumulated nyob rau hauv mitochondria.
Tsiaj sim tau tshwm sim
Mob toxicity: Hauv vivo cov kev tshawb fawb tau pom tias raws li cov tshuaj LD50 mob toxicity tus nqi, nws cov tshuaj toxicity qis dua li ntawm nws niam txiv molecule Rhodamine B. Tus nqi LD50 ntawmRHODAMINE 110yog 140.0mg / kg, thaum LD50 tus nqi ntawm cov tshuaj no yog 89.5mg / kg.
Cov teebmeem hauv lub cev: Tom qab noj cov tshuaj no thiab Rhodamine B, ob qho tib si molecules ua rau lub siab thiab lub raum loj. Cov txiv neej nas tau pom ntau lub siab thiab lub raum loj tom qab kis ntau dua li cov nas poj niam. Testicular hnyav ntawm txiv neej nas muab cov compound no nce.
Pharmacokinetics: Nws cov tshuaj pharmacokinetics tau soj ntsuam los ntawm ob koob tshuaj: kev tswj qhov ncauj (3 thiab 10mg / kg) thiab kev tswj hwm txoj hlab ntshav (3mg / kg). Tom qab kev tswj hwm qhov ncauj, nws qhov nqus tsis nrawm heev thiab nws yuav siv sijhawm ntau dua 2 teev kom nqus tau los ntawm txoj hnyuv mus rau hauv cov hlab ntsha. Qhov siab tshaj plaws plasma concentrations (Cmax) ntawm ob koob tshuaj qhov ncauj yog 283.4 thiab 657.0 ng / mL, raws li, mus txog ntawm 140 thiab 210 feeb. Lub cheeb tsam nyob rau hauv cov tshuaj lub sij hawm nkhaus (AUC) rau ob koob tshuaj yog 138.1 ± 20.3 thiab 444.0 ± 170.8 hng / mL, ntsig txog, qhia tias AUC yog proportional rau qhov ncauj koob tshuaj. Tus nqi tshem tawm (Cl) ntawm ob koob tshuaj hauv qhov ncauj yog 7.94 thiab 8.61 mL / min / kg, raws li.
Kev mob tshwm sim
Lub siab thiab lub raum puas
Vim qhov tseeb tias kev noj cov tshuaj no tuaj yeem ua rau lub siab thiab lub raum loj, kev siv sijhawm ntev - lossis siab - koob tshuaj yuav ua rau cov kabmob no puas tsuaj. Lub siab thiab ob lub raum yog ib qho tseem ceeb hauv metabolic thiab excretory kab mob hauv tib neeg lub cev, thiab kev puas tsuaj tuaj yeem ua rau kev ua haujlwm tsis zoo, uas cuam tshuam rau kev noj qab haus huv tag nrho.
Kev cuam tshuam rau lub cev xeeb tub
Hauv kev sim tsiaj, qhov nce hauv qhov hnyav ntawm cov qe menyuam tau pom nyob rau hauv cov txiv neej nas muab nws, uas yuav qhia tau tias cov tshuaj muaj qee yam cuam tshuam rau lub cev xeeb tub. Kev kis tau mus ntev lossis siab siab - siv koob tshuaj yuav ua rau muaj kev ua txhaum ntawm kev ua me nyuam, xws li cov phev kom muaj nuj nqis tsawg lossis tsis zoo, tsis muaj peev xwm fertility, thiab lwm yam.
Cov teebmeem cytotoxic
Txawm hais tias nws tsis muaj cytotoxicity rau tib neeg lymphoblasts ntawm qhov tsis tshua muaj siab, nws tuaj yeem ua rau tuag ntawm phooj ywg leukemia hlwb ntawm qhov siab. Qhov no qhia tau hais tias cov khoom muaj peev xwm cytotoxic cuam tshuam thiab yuav ua rau muaj kev puas tsuaj rau cov hlwb ib txwm muaj, tshwj xeeb tshaj yog nyob rau hauv cov koob tshuaj siab lossis ntev - siv sijhawm.
Kev ua xua
Raws li cov khoom siv tshuaj txawv teb chaws, cov tshuaj no tuaj yeem ua rau muaj kev tsis haum rau tib neeg lub cev. Cov tsos mob ntawm kev tsis haum tshuaj yuav muaj xws li pob khaus, khaus khaus, ua pa nyuaj, laryngeal edema, thiab lwm yam, uas tuaj yeem ua rau muaj sia nyob - hem thaum mob hnyav.
Lwm yam kev mob tshwm sim
Vim nws cov qauv tshuaj thiab cov khoom siv, nws kuj tseem yuav ua rau muaj lwm yam kev mob tshwm sim, xws li kev puas tsuaj rau lub paj hlwb, lub plab zom mov tsis zoo, thiab lwm yam. Cov kev tshwm sim tshwj xeeb thiab qib ntawm cov kev mob tshwm sim no yuav txawv ntawm ib tus neeg mus rau ib tus neeg, thiab kuj tseem muaj feem xyuam rau kev siv tshuaj thiab kev siv.
Kev ceev faj rau kev siv
Rau kev tshawb fawb lub hom phiaj nkaus xwb
Tam sim no nws tsuas yog siv rau kev tshawb fawb txog kev tshawb fawb thiab tsis siv los ua tshuaj, tsev neeg cov tshuaj thaub qab, lossis rau lwm lub hom phiaj. Nyob rau hauv kev tshawb fawb thwmsim, nws yog ib qho tsim nyog yuav tsum nruj me ntsis ua raws li cov txheej txheem kev sim thiab cov txheej txheem kev khiav hauj lwm kom tsis txhob muaj kev cuam tshuam tsis tsim nyog thiab kis kab mob.
Zam txhob raug-ntev ntev
Vim nws muaj peev xwm cytotoxicity thiab kev puas tsuaj rau lub cev, ntev - kis tau los yog siab - siv koob tshuaj yuav tsum raug zam. Thaum lub sij hawm kev sim, qhov ntau npaum li cas thiab lub sij hawm ntawm kev siv yuav tsum tau tswj nruj me ntsis kom txo tau cov kev puas tsuaj rau cov tsiaj sim thiab tib neeg.
Ua tib zoo saib xyuas tus kheej
Thaum siv, cov cuab yeej tiv thaiv tus kheej tsim nyog xws li hnab looj tes, qhov ncauj qhov ntswg, thiab tsom iav yuav tsum hnav. Tsis txhob kov ncaj qha rau ntawm daim tawv nqaij thiab qhov muag kom tsis txhob nqus los yog nqus tau.
Kev khaws cia thiab pov tseg
Cov tshuaj no yuav tsum tau muab khaws cia rau hauv qhov chaw qhuav, txias, zoo ventilated, deb ntawm qhov hluav taws kub thiab cua sov. Cov khoom pov tseg yuav tsum tau muab pov tseg raws li cov kev cai ntawm lub tuam tsev tiv thaiv ib puag ncig hauv cheeb tsam kom tsis txhob muaj kuab paug rau ib puag ncig.
FAQ
Puas yog soluble hauv dej?
Solubility hauv dej:me ntsis soluble. 77000 l/(mol.cm) (ntawm lambda max.)
Dab tsi yog qhov wavelength ntawm nws?
Nws yog ib qho rhiab heev thiab xaiv substrate rau kev soj ntsuam cov proteinases hauv kev daws lossis hauv cov hlwb nyob. CovKev tawm suab wavelength yog 498nm thiab emission wavelength yog 521nm.
Dab tsi yog rhodamine dye siv rau?
Rhodamine dye, xanthene dye, feem ntau sivraws li tus neeg taug qab (Rhodamine WT) hauv kev ntsuas dej zoo. Nws tau siv dav raws li 'lub sij hawm mus ncig' dye rau ob qho tib si dej saum npoo av thiab hauv av nrog lub peev xwm los stain cov ntaub ntawv organic.
Dab tsi yog rhodamine 123 siv rau?
Rhodamine 123, cell -permeating fluorescent dye, tau siv davlos soj ntsuam cov mitochondrial membrane muaj peev xwm ntawm cov cell nyob raws li daim nyias nyias muaj peev xwm - nyob ntawm tsub zuj zuj.
Cim npe nrov: Rhodamine 110 CAS 13558-31-1, lwm tus neeg, manufacturers, hoobkas, lag luam wholesale, yuav, nqe, tej, kev muag khoom