Nruab nrab liab hmoov, tseem hu ua Dimethylliaminophenazine chloride lossis toluene liab. Nws yog ib qho kua qaub - Base taw qhia, CAS 553}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}. Ntsuab crystalline hmoov, yooj yim soluble hauv dej thiab liab hauv cov xim, soluble hauv ethanol thiab daj hauv cov xim. Nws cov kev daws tau siv los ua vacuoles hauv cov hlwb, uas tuaj yeem txheeb xyuas lub hom phiaj ntawm tes. Nyob cov hlwb yog stained liab, thaum tuag hlwb tsis hloov xim. Qhov no yog rov qab mus rau Tai Yias Lan. Mas siv cov kua qaub-pib ua, nws cov xim pH xim hloov chaw ntau yog 6.8-8.0, thiab nws cov xim muaj ntau los ntawm liab mus rau txiv kab ntxwv daj. Alkalinity qhia rau dej, Nitrite, thiab zis; Kev ntsuas hydrolysis siv rau cov rog hauv kev teeb tsa kev ua haujlwm; Nissl staining ntawm cov hlab hlwb. CO siv nrog Jiana ntsuab rau hauv cov vitro staining ntawm cov ntshav; Photometric txiav txim siab ntawm iodine ions; Npaj ntawm nruab nrab daim ntawv ntsuas liab. Ib qho ntxiv, nws kuj tseem tuaj yeem siv los ua cov tshuaj oxidation-txo cov cim, kev saib xyuas liab, nruab nrab nruab nrab liab, thiab lwm yam.
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Tshuaj Cov Qauv |
C15h177cln4 |
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Muaj pes tsawg leej |
289 |
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Molecular hnyav |
299 |
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m/z |
288 (100.0%), 289 (16.2%), 290 (32.0%), 291 |
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Kev Ntsuam Xyuas |
C, 62.39; H, 5.39; Cl, 12.28; N, 19.40 |
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Nruab nrab liab hmoovPuas yog feem ntau siv qhia tau siv cov kua qaub - bex} - kev tawm tsam txo qis. Cov txheej txheem ntawm kev tsim cov nruab nrab liab los ntawm n, n- nitrosyl}} p} p} p} p} p} p} p} p} p} p} p} p} p} p} p} p} p} p} p} p} p} p} p} p} p} p} p} p} p} p} p} p} p} p} p} p} p} p} p} p} p} p} p} p} p} p} p} p} p} p} p} p} p} p} p} p} p} p} p} p} p} p} p} p} p} p} p} p} p} p} p} p}? Cov hauv qab no yog cov txheej txheem ntxaws ntxaws thiab tshuaj sib txig sib luag:
Kev npaj ntawm P - nitrosamine hydrochloride: Firroly}} nitrosamine nrog hydrochloric acid los tsim p ing- nitrosamine hydrochloride.
Tshuaj Muaj Tshuaj:
H2N-C6H4-} tsis muaj2 + HCL → h2N-C6H4-} tsis muaj2· HCL
N. N {{- dimethylation: hnov p-}}}}}}}}} dimethylation.
Tshuaj Muaj Tshuaj:
H2N-C6H4-} tsis muaj2· Hacl + c3H7Tsis muaj + c6H15N → n, n - dimethyl {{1} p} p} p} nitrosposoanloride
Kev npaj ntawm Toluene - 2,4 {4 {{{{}}}}}} dolreales nyob rau hauv cov sulfuric acid kom tau p-nitrotoluene. Tom qab ntawd, siv cov neeg sawv cev me me xws li cov hlau cov hmoov thiab hydrochloric acid los txo cov P-Nitrotoluene tau txais hauv cov kauj ruam dhau los mus rau Toluene-2,4-sib.
Tshuaj Muaj Tshuaj:
C6H5-} tsis muaj2 + H2 + FE + HCL → C6H3(NH2)-2H
Coupling kev xav: n, n- dimethyl {}} p- Permealyling tau txais cov tshuaj tua kab mob (xws li cov tshuaj yej (xws li tshuaj yej lossis pyridine) rau coupling tshuaj.
Tshuaj Muaj Tshuaj:
N. N {- dimethyl {}} p}} p}}} →} {}} hcl + hcl + catalyst
Purification: purify cov tshuaj tiv thaiv cov khoom lag luam los ntawm kab chromatography los yog recrystallization kom tau txais qhov siab {}} purity nruab nrab liab.
Ntxiv Dimethylaniline mus rau 5 Degree Hydroous Sol a axoous daws, thiab tom qab ntawd ntxiv cov tshuaj tiv thaiv aqueous, nrog rau qhov ntsuas kub ntawm 5-10 degree:
Tom qab nplawm rau 1 teev, lim thiab crystallize P - nitrosodimethralide. Ntxuav nrog ib qho me me ntawm cov dej thiab teem ib sab. Dissolve ferrous sulfate nyob rau hauv dej kub ntawm 90 degree, ces ntxiv 2,4 {} Diam p-nitroshlosodaniline hydrochloride. Do rau cov tshuaj tiv thaiv:
Tom qab 1 teev, ntxiv ib qho tsim nyog ntawm hydrochloric acid thiab lim. Txom hydrogen chloride pa mus rau hauv cov tshuaj, cia nws sawv ntsug, tos rau crystallization kom tiav, lim, thiab ua kom qhuav tau cov khoom lag luam nruab nrab.
Nruab nrab liab hmoov, Tshuaj lom neeg lub npe 3-amino-7-methylfhaylala (lossis 3-amino-6-amino-6-dim) c ₁ H ₁ hard Nws yog cov hmoov tsaus ntsuab crystalline uas yooj yim soluble hauv dej (liab) thiab ethanol (daj tsis muaj zog alkalinity thiab ib hom ntawm 6.4-8.0 (liab rau daj). Raws li cov xim penazine classic, nruab nrab liab tau siv dav hauv cov khoom siv tshuaj lom neeg, lom neeg lossis lwm thaj teb. Nws cov ntawv thov tub ntxhais tseem tuaj yeem ua tau ua kom tiav rau hauv cov hauv qab no:
Ib qho ntawm cov tub ntxhais muaj nuj nqi ntawm nruab nrab liab yog ua ib qho kev hloov pauv tsis muaj zog, pT 6.8.0), maj mam hloov ntawm liab mus rau txiv kab ntxwv daj. Cov xeeb ceem no ua rau nws lub cuab yeej zoo tagnrho rau kev saib xyuas cov acidity thiab alkalinity ntawm kev daws teeb meem hauv chaw soj nstuam thiab kev lag luam:
Kev xa ntawv: Hauv Titrws tsom xam, nws yog feem ntau siv los qhia qhov kawg ntawm kev daws teeb meem lossis nruab nrab kev daws teeb meem. Piv txwv li, hauv kev sim ntawm kev sim siv hluav taws xob hydrochloric, thaum txoj kev daws teeb meem, nws hloov los ntawm liab mus rau daj, qhia txog qhov xaus xaus.
Kev sim tshuaj: tuaj yeem siv los tshawb xyuas cov alkalinity ntawm cov kua xws li dej, etrite, hauv kev ua zaub mov noj, hauv kev ua zaub mov noj ua khoom noj, ua kom cov khoom siv tau ruaj khov.
Kev Tswj Xyuas Ib puag ncig: Hauv kev kho kom muaj dej rau ib puag ncig, nws tuaj yeem qhia txog qhov ua tiav ntawm kev ua kom tiav ntawm kev tsis muaj kev nyab xeeb (xws li 6-9), thiab zam kom tsis txhob ua phem rau cov kab ke kab laj kab.
Nruab nrab liab yog qhov tsis tseem ceeb ntawm cov cuab yeej siv roj ntsha, yog li xaiv cov khoom siv roj ntsha li ntawm cov hlwb los ntawm cov kab mob uas muaj sia:
Kev Kuaj Cell
Kev nruab nrab liab staining yog ib txoj kev classic rau kev tshawb pom cov kev sib kis ntawm kev. Lub vacuoles ntawm cov leeg muaj acidic thiab tuaj yeem nqus nruab nrab liab thiab castociate cations, ua rau vacuoliate cations, ua cov vacuoles tshwm sim, Cov hlwb tuag tsis tuaj yeem tswj hwm lawv cov qauv clamp vim yog cov qauv tiv thaiv protoplast. Nruab nruab nrab liab cations khi rau kev them nyiaj tsis zoo thiab nuclei, ua rau lub nuclei rau cov vacuoles. Lub ntsiab cai no tau siv dav rau:
Tshuaj Kev Kuaj Tshuaj Tshuaj Tox: Txheeb xyuas cov kev tua cov khoom siv tshuaj kho mob rau cov qog nqaij hlav cancer los ntawm kev siv liab.
Organizology Engineering: Tshawb cov kev ua si ntawm cov qia cell condures thiab kev ua kom zoo ntawm tes nthuav dav.
Khoom noj khoom haus micobiology: kev qhia sai sai ntawm cov kev muaj sia nyob ntawm cov kab mob muaj sia nyob hauv cov zaub mov, xws li cov kev ua si uas kuaj tau ntawm cov kab mob acids acidic.
Orgymeting
Lysosome Staining: nruab nrab liab tuaj yeem cim cov acidic ib puag ncig ntawm Lysosomes thiab yog siv los kawm txog cov kab mob uas cuam tshuam lossis lysosome ntsig txog cov kab mob.
Golgi Apparatus Staining: Ua ke nrog Janus ntsuab B, nruab nrab liab tuaj yeem txhim kho qhov sib piv ntawm cov khoom siv golgi thiab pab txhawb cov kev soj ntsuam ntawm lub tsom iav micpocopic.
Neuronal Stain yog siv los sau npe Nissl lub cev ntawm neurons, pab hauv kev kawm ntawm neurodegenerative cov kab mob neurodegener rau cov kab mob alzheimer.
Rog Hydrolysis Qhov Ntsuas: Cov nqaij mos mos, nruab nrab liab tuaj yeem qhia cov kev ua si li lipase thiab muaj kev cuam tshuam txog cov muaj rog los ntawm kev hloov xim.
Kev Tshawb Fawb Txog Cov Ntshav thiab Kev Tshawb Fawb
Nyob rau hauv vitro hauv vivo ntawm cov ntshav: nruab nrab liab ua ke nrog Jianna ntsuab tuaj yeem paub cov ntshav liab ntawm cov ntshav liab (xws li Malaria Parasites) hauv cov ntshav.
Lub tshuab txhaj tshuaj tiv thaiv kab mob: Raws li cov keeb kwm yav dhau los ntawm cov xim liab, nruab nrab ntawm cov cim fuorescent thiab txhim kho cov duab fluorescent ntawm immunohistochemical.
Kev txheeb xyuas muaj: kev kho kom pom tseeb ntawm cov ions tshwj xeeb thiab tshuaj
Cov tshuaj lom neeg ntawm nruab nrab liab ua rau nws muaj kev ntsuas kev lag luam hauv kev lag luam, tshwj xeeb haum rau ion nrhiav kom zoo thiab lav
1. Iodine ion photometric kev txiav txim siab
Nruab nruab nrab liab daim ntawv cov xim av nrog iodide ions (Kuv ⁻), thiab nws txoj kev nqus tau yog ib feem ntawm iodide ions. Los ntawm kev siv spectrophotometry, iodine cov ntsiab lus hauv cov qauv dej lossis cov zaub mov muaj peev xwm tau txiav txim siab, xws li tshuaj xyuas Iodine Nodization cov txheej txheem.
2. Cov hlau ion Nrhiav tau
Nruab nruab nrab liab tuaj yeem tsim cov xim av ion complexes nrog mercury thiab indium ions hauv dej khib nyiab lossis ores.
Piv txwv li, hauv cov khoom siv khib nyiab hluav taws xob, liab nruab nrab tuaj yeem kuaj pom sai sai rau cov ntaub ntawv uas muaj inrium.
3. Nitrite nrhiav tau
Nruab nrab liab hmoovreacts nrog nitrite los tsim cov txiv kab ntxwv liab, uas tuaj yeem ntsuas cov nqaij nitrite (xws li cov tshuaj tua kab mob kom zoo nkauj (xws li EU txwv ntawm 0.5-3 mg / kg).
Cov chaw kuaj mob thiab kev kuaj mob patholosis: ib tus kab mob nrhiav kom kuaj tau thiab kho tus mob
Daim ntawv thov ntawm Neutral liab nyob rau hauv lub tsev kho mob xa mus rau kev kuaj mob thiab kev tshawb fawb patological, muab cov cuab yeej pom kev rau kev kuaj mob:
1. Kev kuaj ntshav
Lub nruab nrab liab stain txoj kev yog siv kom paub qhov txawv ntawm cov haujlwm thiab necrotic cheeb tsam ntawm cov qog ntawm cov qog. Piv txwv li, tom qab phais cov qog ntawm cov qog, cov kev ua haujlwm ntawm tes tuaj yeem raug ntsuas sai sai los qhia ntxiv kev kho mob (xws li seb puas yuav nthuav tawm cov kev hloov kho tshiab).
Kev kuaj mob
Nruab nruab nrab liab tuaj yeem siv los kos cov kua qaub cov kab mob ntawm cov kab mob lossis cov kab mob fungi thiab pab rau kev kuaj kab mob kis tau. Piv txwv li, hauv kev kuaj cov hnoos qeev, nruab nrab liab staining tuaj yeem paub qhov txawv ntawm mycobacterium tuberculosis thiab txhim kho kev kuaj mob tus kws kho mob.
Saib Xyuas Lub Cell Kev Saib Xyuas
Nyob rau hauv liv xovtooj ntawm tes, nruab nrab liab staining yog siv los ntsuas tus nqi ciaj sia taus thiab muaj peev xwm muaj peev xwm hloov hlwb. Piv txwv li, hauv kev kho lub hlwb, kev sib cav ntawm qia hlwb nyob rau hauv cov txheej txheem corneal hauv lub tsev liab plhaw.
Nruab nrab liab tau dhau los ua cov reagent classic hauv biology thiab chemistry qhia vim nws tau ua haujlwm yooj yim thiab tus nqi qis
Kev sim Cell Biology
Txoj kev nruab nrab liab tsuas yog siv dav hauv kev qhia cov kev sim kom pab cov tub ntxhais kawm nkag siab ntawm cov qauv cell thiab ua haujlwm. Piv txwv li, los ntawm kev saib cov kab mob hlwb Epidermal tsuas yog cov tub ntxhais kawm nruab nrab, cov tub ntxhais kawm tuaj yeem nkag siab txog kev sib raug zoo ntawm cov av nplaum thiab kev ua haujlwm ntawm tes.
Microbiology Kev Qhia
Hauv microbial cultivation sim, nruab nrab liab yog siv los paub qhov txawv ntawm cov kab thiab tuag taus ntawm txoj kev ntsuas cov kab mob microbial ntsuas.
Kev Tshawb Fawb ntawm Cov Khoom Siv Tshuaj
Nruab nruab nrab liab, raws li ib tug qauv sib txuas, yog siv los kawm cov xim hloov pauv thiab cov qauv kev sib raug zoo ntawm kev txhim kho phenazine rau kev txhim kho cov ntsuas tshiab.
Cim npe nrov: Nutral Red hmoov CAS 553-24-2, cov neeg xa khoom, chaw tsim khoom, Hoobkas, lag luam Wholesale, yuav, nqe, loj, rau muag