Ua casthydroethidium CAS 104821-25-2

Uahirtroethidium, Tus lej lej 104821-25-2,, molecular formula c21N3, nrog qhov muaj tseeb molecular ceeb thawj cov kev siv roj ntsha. Nws feem ntau pom tias yog cov hmoov nplua crystalline zoo li qub los ntawm cov paj yeeb mus rau xim liab. Cov xim tshwj xeeb no ua rau nws yooj yim kom paub txog hauv chav kuaj thiab muaj keeb kwm yav dhau los ua kev soj ntsuam fluorescent. Hauv kev tshawb fawb thiab kev tshawb fawb roj ntsha, feem ntau nws yog feem ntau siv los ua kev sojntsuam cov pa oxygen, tshwj xeeb tshaj yog nyob rau hauv kev kuaj cov pa oxygen Qhov xim zas no tuaj yeem nkag mus hauv hlwb thiab lub dehydrogenate los ua cov kab mob bromide. Qhov kev sojntsuam no tau siv dav hauv NK Cell thiab ua ib qho zas tseem ceeb kom paub txog cov kws tshaj lij ntawm tes thiab hypoxia hauv cov qog.

Uahirtroethidium, raws li lub xiav xiav fluorescent soj ntsuam uas tuaj yeem txig cov qe, ua lub luag haujlwm tseem ceeb hauv kev ua liaj ua teb thiab tshuaj. Nws cov teeb meem fluorescence ua kom muaj peev xwm los kuaj xyuas cov qauv superoxide (ORO

1. Cell Duab

Dihydroethylene, raws li fluorescent sojntsuam, tuaj yeem sau cov kab mob fluorescent, tuaj yeem sau cov kab mob hlwb thiab khi rau DNA, emitting liab fluorescence. Yog li ntawd, nws tau siv dav hauv cell kev xav siv tshuab los saib xyuas cov xwm txheej redox nyob rau hauv cov cell hauv tiag- lub sij hawm. Los ntawm cov khoom siv xws li fluorescence lossis ntws cytometry, yog li kev faib cov xov tooj ntawm cov hlwb hauv lub hlwb lossis cov xeev pathological.

2. Redox Xeev Nrhiav Tau

Fluorescence Profertes ntawm ethylene ihydrogen Ingot ua nws lub cuab yeej zoo tagnrho rau kev ntes lub xeev redox. Tsis pub dhau lub hlwb, diydroethylenediamine tuaj yeem oxidized los ntawm superoxide selylened dab, uas tom qab ntawd khi rau DNA thiab emits fluorescence. Yog li no, los ntawm kev siv fluorescence ntawm dihydroethylenediamine, theem ntawm intracellular cov tshuaj superoxide superoxide me ntsis, yog li tshuaj xyuas Redox xwm txheej ntawm cov hlwb. Txoj kev kuaj pom no muaj qhov zoo ntawm cov kev xav zoo, muaj qhov tshwj xeeb, muab cov cuab yeej muaj zog rau kev kawm cov kev hloov pauv ntawm tes.

3. Kev Tshawb Fawb Hlua

Dihydroethylene muaj ntau cov kev siv hauv cov kev tshawb fawb tu siab. Vim yog lub siab redox theem siab ntawm cov qog hlwb, ua rau cov cim qog ua tau zoo rau kev kuaj mob thaum ntxov thiab kho cov qog nqaij hlav. Ib qho ntxiv, dihihleethylenediamine kuj tseem siv tau los kawm cov thev naus laus zis ntawm cov qog ua rau pom cov qog nqaij hlav tshwm sim thiab kev loj hlob.

4. Tshuaj Ntsuam Xyuas

Dihydroethylenediamine kuj tseem ua lub luag haujlwm tseem ceeb hauv kev kuaj tshuaj. Ntau cov tshuaj, thaum muaj kev cuam tshuam kho cov teebmeem, kuj tseem muaj feem cuam tshuam rau Redox lub xeev ntawm cov hlwb. Yog li no, los ntawm kev siv fluorescence ntawm dihydroethylenediamine, kev cuam tshuam ntawm cov tshuaj roxular boxfience tuaj yeem raug ntsuas, thiab cov peev xwm kho cov tshuaj yuav raug tshuaj ntsuam. Ib qho ntxiv, dihirothylenediamine kuj tseem siv tau los kawm txog kev sib txuam mechanism ntawm cov tshuaj thiab cov qog noj ntshav, muab kev txhawb zog rau kev tsim tshuaj thiab chaw kho mob.

5. Kev ntsuam xyuas biosafety

Dihydroethylene kuj tseem siv tau nyob rau hauv kev ntsuas biosafety. Nyob rau hauv kev cuam tshuam ntawm ib puag ncig cov pa paug thiab co toxins, lub xeev redox ntawm cov hlwb yuav hloov. Los ntawm kev kuaj pom cov roj fluorescence ntawm dihydroethylenediamine, qhov kev cuam tshuam ntawm cov tshuaj ntawm cov xov tooj ntawm tes redebular redebular redebular redebular redebular redebular redebular redebular redebular redebular redebular redebular redebular redebular redebular redebular redebular redebular redebular redebular redebular redebular redebular redebular redebular redebular redebular redebular redebular redebular redebular redebular redebular redebular redebular redebular redox Hom no yog qhov tseem ceeb tshaj plaws rau kev txheeb xyuas cov kev pheej hmoo ntawm cov pa phem ib puag ncig thiab ua kom tib neeg kev noj qab haus huv.

Cov kauj ruam ntxaws ntxaws thiab coj cov tshuaj sib txig sib luag rau cov synthesis ntawmUahirtroethidiumHauv chav kuaj yog tus txheej txheem koom nrog cov tshuaj pleev organic hluav taws xob synthesis.

1. Kev npaj cov khoom siv raw

Pib cov khoom siv: Xaiv cov khoom pib tsim nyog, uas tej zaum yuav yog qhov sib txuas nrog lub nplhaib benzene thiab pawg amino.

Cov kuab tshuaj thiab catalysts: xaiv cov kuab tshuaj uas tsim nyog (xws li ethanol, thiab lwm yam) raws li cov tshuaj tiv thaiv hlau) raws li cov tshuaj tiv thaiv.

2. Thawj kauj ruam kev tawm tsam: Taw qhia thiab hloov kho ntawm Benzene Ring

Yam tshuaj tiv thaiv: kev hloov pauv kev coj ua lossis txuas cov tshuaj tiv thaiv ntawm cov ntxhiab hydrocarbons.

Cov kauj ruam tshwj xeeb: nyob rau hauv kev txiav txim ntawm catalyst, xws li phenylbboronic acid, halogenated benzene, thiab lwm yam) los qhia tus nplhaib benzene.

Tshuaj Muaj Tshuaj: Vim yog tus qauv tsis paub ntawm cov reactants thiab cov khoom lag luam, cov khoom siv dav dav yog siv los sawv cev:

Pib Khoom + Benzene Ring Qhia Taw Qhia Reagent → Intermed Inflmed Khoom 1

3. Thib ob kev hloov pauv: Taw qhia lossis hloov kho cov amino pab pawg

Hom Riam: Kev ua txhaum kev tawm tsam lossis amine hloov kev xav.

Cov kauj ruam tshwj xeeb: kev siv cov xwm txheej uas tsim nyog, tshuaj xyuas cov khoom nruab nrab 1 nrog amination reagents (xws li amines, thiab lwm yam) ua pab pawg amino.

Tshuaj Muaj Tshuaj:

Cov Khoom Nruab Nrab Nruab Nrab 1+ kev noj nyiaj reagent → Intermediaps Khoom 2

4. Peb txoj kev hloov siab tshiab: hydrogenation cov tshuaj tiv thaiv

Yam tshuaj tiv thaiv hom: Hydrogenation cov tshuaj tiv thaiv.

Cov kauj ruam tshwj xeeb: nyob rau hauv kev txiav txim ntawm catalysts (palladium, thiab lwm yam khoom 2 yog hydrogenated kom tau txais Dihydrohlene Ingot lossis nws qhov analogues.

Tshuaj Muaj Tshuaj:

Cov khoom lag luam nruab nrab 2+ h2} → dihyroethylene Ingot (lossis zoo sib xws)

5. Purification thiab cim

Purification: purify cov khoom los ntawm cov hau kev xws li recrystallization thiab kem chromatography.

Cov xeeb ceem: Siv cov txuj ci xws li loj spectrometry, infrared spectroscopy, thiab nuclear sib nqus kev ua kom pom cov khoom lag luam thiab paub meej tias nws cov qauv thiab kev coj dawb huv.

Uahirtroethidium(DHE) yog kev sojntsuam fluorescent dav siv nyob rau hauv kev tshawb fawb txog kev muaj roj ntsha. Nws cov teeb meem fluorescence muaj nws qhov tseem ceeb hauv kev ntes cov khoom siv oxygen reions (tshwj xeeb tshaj yog cov ingoxide superox). Cov hauv qab no yog cov lus qhia ntxaws ntxaws rau cov khoom fluorescence fluorescence ntawm ethylene dihydrogen agots:

Siv cov tshuaj ntsuam xyuas tes ntawm no uas yog siv los kuaj cov tshuaj yej xiav fluoroxide me me (O2-) hauv cov hlwb. Cov hauv qab no yog lub hauv paus ntsiab lus ntawm nws cov ntaub ntawv thiab nws cov ntaub ntawv thov hauv kev tshawb fawb txog kev tshawb fawb txog

Xyuas kom tau lub hauv paus ntsiab lus
DHE tuaj yeem ua kom lub cev qhuav dej los ntawm inhoxide superoxide anion los tsim cov kab (piv txwv li, kev coj tsis ncaj rau kev ua neej. Cov kab mob tawm tuaj yeem khi rau RNA lossis DNA los ua cov roj liab liab. Thaum lub incoxide superoxide Act qib siab dua, ntau dua kev coj ua tau tsim thiab cov npliab plab zom mov; Hloov siab, nws yog qhov tsis muaj zog. Qhov no tso cai rau kev kuaj pom ntawm superoxide anion qib nrog DHE. At the same time, DHE itself fluoresces blue in the cytoplasm until it is oxidized, and then it will be inserted into the cellular DNA, staining the nucleus a bright fluorescent red.

Cov piv txwv thov

Kev kuaj pom ntawm Ros ntau lawm hauv daim ntawv foob: daim nplooj daim hlab daim ntaub lim ntaub ntawv tau npaj thiab ua kom hnyav nrog dhe. Cov ntu yog pom tias siv lub tsom iav fluorescence thiab qhov feem pua ​​ntawm kev ntsuas morphometric los ntsuas cov roj oxygen) ntau lawm.

Txhawm rau tshawb cov kev hloov pauv hauv antioxidant enzymes thiab glutathione redoxsion redoxs ntawm cd {{} yog sau npe siv dhe thiab mam li assayed los ntawm ntws. Cov txiaj ntsig tau pom tias cov ntsiab lus oxygen tau muaj txiaj ntsig rau tiam superoxide thiab peroxide, nrog tus nqi siab dua ntawm peroxide lub xeev.

Cov ntsiab cai saum toj no thiab cov ntaub ntawv thov cov ntaub ntawv qhia tias SHE, ua ib qho kev tshawb tshuaj lom zem superorescent fluorescent, muaj ntau cov kev siv hauv kev tshawb fawb txog kev muaj roj ntsha. Nws tsis tuaj yeem siv los ntsuas cov qib ntawm oxidative kev ntxhov siab hauv cov tshuab ntawm cov kab mob thiab cov tshuaj tiv thaiv kab mob antioxidant.

► Tsim kev siv hluav taws xob -}} daim ntawv loted

Yuav kom pab tau nyob rau hauv vivo dynaming tracking ntawm superoxide, cov kws tshawb nrhiav tau tsim ib qho ¹¹c -}}}}}}}}}}}}}}}}}}}}}}}. Los ntawm kev hloov kho cov txheej txheem kev hloov kho (qhia txog cov kab sib txawv ntawm lub nplhaib Benzene), cov khoom siv tshuaj lom neeg thiab cov yam ntxwv ntawm DHE tau tshwj tseg. Tsiaj cov duab qhia tau qhia tias qhov kev sojntsuam no ua rau muaj kev sib txuam nrog cov ntaub so ntswg zoo nkauj nrog cov ntaub so ntswg zoo nrog rau kev sib cuam zoo (r {}}, p<0.001), thereby providing a novel tool for early diagnosis of cardiovascular diseases.

► Kev loj hlob ntawm Mitochondria - nrhiav cov phiaj xwm

Txhawm rau daws cov kev xav tau rau Mitochondrial Superoxide Superoxide SuperSox liab (ib mitochondria {}} ib cheeb tsam ntawm dhe). Qhov kev sojntsuam no Mitochondphine ntawm ib qho chaw ncig ua si moami, nrog nws cov khoom oxidation, nrog nws cov tshuaj oxidation emitting txiv kab ntxwv (510/580 nm). Hauv Parkinson tus kab mob qauv, Mitosox liab tau qhia tias Mitochondrial SuperSroxide ntau tshaj plaws, tshwm sim ntxov dua li apoptotic parters. Qhov no muab lub biomarker rau kev cuam tshuam thaum ntxov hauv Nurodegenerative cov kab mob.

Cim npe nrov: Ua Cas 104821-25-2, Tus Neeg Tsim Khoom, Chaw Tsim Tshuaj, Chaw Tsim Tshuaj, Lag Luam Wholesale, Yuav, Nqe, loj, rau muag