Fluorescamine, chemical formula C17H10O4, molecular hnyav 278.26, CAS naj npawb 38183-12-9, yog ib qho tseem ceeb tshuaj reagent dav siv nyob rau hauv lub teb ntawm biochemistry thiab analytical chemistry. Nws feem ntau muaj nyob rau hauv daim ntawv ntawm ib tug dawb mus rau tawm dawb hmoov, thiab tej zaum kuj yuav tshwm daj ntseg daj. Nws daim ntawv hmoov ua rau nws yooj yim rau kev khiav lag luam thiab hnyav hauv chav kuaj. Solubility nyob rau hauv dej yog tsawg, tshwm me ntsis soluble. Txawm li cas los xij, nws tuaj yeem yaj tau zoo hauv ntau cov kuab tshuaj organic xws li acetonitrile, acetone, ethanol, chloroform, thiab dimethyl sulfoxide. Cov yam ntxwv solubility no tso cai rau cov tshuaj fluorescent amines tau npaj thiab ua rau hauv chav kuaj los ntawm kev xaiv cov kuab tshuaj uas tsim nyog. Nws tsis muaj cov khoom fluorescence nws tus kheej, tab sis nws tuaj yeem cuam tshuam nrog cov tebchaw uas muaj cov amino pawg thawj (xws li cov proteins, peptides, amino acids, thiab lwm yam) los tsim cov tebchaw uas muaj zog fluorescence. Cov yam ntxwv no ua rau nws yog ib qho tseem ceeb fluorescent derivative reagent. Muaj peev xwm hnov mob nrog cov thawj amino pawg ntawm cov amino acids nyob rau hauv cov mob me me kom tsim tau ruaj khov, siab fluorescence, tsis tshua muaj keeb kwm yav dhau. Qhov no complex muaj qhov rhiab heev nyob rau hauv fluorescence nrhiav tau thiab yog li no dav siv rau kom muaj nuj nqis tsom xam thiab nrhiav kom tau cov amino acids. Hauv kev txheeb xyuas cov protein, nws kuj tuaj yeem ua rau cov amino pawg tseem ceeb ntawm cov protein los tsim cov fluorescent derivatives. Los ntawm kev txheeb xyuas qhov kev siv fluorescence ntawm cov derivatives no, kev soj ntsuam ntau ntawm cov ntsiab lus protein tuaj yeem ua tiav.
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Tshuaj Formula |
C17H10O4 |
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Exact Mass |
278 |
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Molecular Luj |
278 |
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m/z |
278 (100.0%), 279 (18.4%), 280 (1.6%) |
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Elemental Analysis |
C, 73.38; H, 3.62; O, 23.00 |
Fluorescamineyog ib qho tseem ceeb tshuaj reagent nrog dav siv nyob rau hauv lub teb ntawm biochemistry thiab analytical chemistry.
1. Fluorescence nrhiav thiab tsom xam
Amino acid nrhiav kom tau: Nws tuaj yeem hnov mob nrog cov thawj amino pawg ntawm cov amino acids nyob rau hauv cov mob me me los ua kom ruaj khov, siab fluorescence, thiab cov keeb kwm tsis tshua muaj. Cov complex no muaj qhov rhiab heev hauv kev tshawb nrhiav fluorescence thiab feem ntau yog siv rau kev txheeb xyuas ntau ntawm cov amino acids.
Kev tsom xam cov protein: Nws kuj tuaj yeem cuam tshuam nrog cov thawj amino pawg ntawm cov proteins los tsim cov fluorescent derivatives, uas yog siv rau kev tshawb nrhiav kom muaj nuj nqis thiab kev tshawb fawb ntawm cov proteins. Los ntawm kev txheeb xyuas qhov kev siv fluorescence ntawm cov derivatives no, cov ntsiab lus ntawm cov protein thiab cov qauv kev hloov pauv tuaj yeem raug ntsuas.
2. Cov kua dej chromatography fluorescence kuaj pom
Kev ua haujlwm siab ua kua chromatography (HPLC) ua ke nrog kev kuaj pom fluorescence yog cov txheej txheem siv ntau hauv kev tshuaj xyuas biochemical. Raws li fluorescent derivative reagent, nws tuaj yeem txhim kho cov teeb liab fluorescence ntawm lub hom phiaj sib txuas, txhim kho qhov rhiab heev thiab kev xaiv ntawm kev kuaj pom. Cov thev naus laus zis no tau siv dav hauv kev lag luam xws li proteomics thiab tshuaj metabolism.
3. Protein sequencing thiab structural tshawb fawb
Hauv kev sib txuas ntawm cov protein thiab cov qauv kev tshawb fawb, nws tuaj yeem siv los sau cov haujlwm tshwj xeeb lossis cov seem ntawm cov protein, pab cov kws tshawb fawb txiav txim siab qhov sib lawv liag thiab cov qauv ntawm cov protein. Txoj kev sau npe no pab kom nkag siab txog kev ua haujlwm thiab kev cuam tshuam ntawm cov proteins.
4. Kev kuaj xyuas cov enzymes proteolytic
Muaj peev xwm hnov mob nrog substrates ntawm proteolytic enzymes los tsim cov khoom fluorescent. Los ntawm kev saib xyuas cov fluorescence siv cov kev hloov pauv ntawm cov khoom no, cov haujlwm thiab cov yam ntxwv ntawm cov enzymes proteolytic tuaj yeem raug soj ntsuam. Qhov no yog qhov tseem ceeb heev rau kev kawm cov catalytic mechanism ntawm enzymes thiab tshuaj ntsuam inhibitors.
1. Kev tshuaj xyuas tshuaj
Hauv cov txheej txheem ntawm kev txhim kho tshuaj, nws tuaj yeem siv los tshuaj xyuas cov hom phiaj tshuaj. Los ntawm kev khi thiab ua rau lub hom phiaj, fluorescent amines tuaj yeem nthuav tawm cov kev sib cuam tshuam ntawm cov tshuaj thiab cov hom phiaj, muab cov ntaub ntawv tseem ceeb rau kev tsim tshuaj thiab kev ua kom zoo.
2. Kev tshawb fawb tshuaj metabolism
Nws kuj tseem siv tau rau kev tshawb fawb txog tshuaj metabolism. Los ntawm kev sau npe cov pab pawg ua haujlwm tshwj xeeb ntawm cov tshuaj lossis lawv cov metabolites, fluorescent amines tuaj yeem pab cov kws tshawb fawb taug qab cov txheej txheem metabolic thiab cov nqi ntawm cov tshuaj hauv lub cev. Qhov no yog qhov tseem ceeb rau kev ntsuas kev nyab xeeb thiab kev ua tau zoo ntawm cov tshuaj.
Lwm daim ntawv thov
1. Kev saib xyuas ib puag ncig
Txawm hais tias nws qhov kev thov ncaj qha hauv kev saib xyuas ib puag ncig yog txwv, nws cov yam ntxwv ua cov fluorescent derivative reagent muab txoj hauv kev tshiab rau kev saib xyuas ib puag ncig. Los ntawm kev sib txuas nrog lwm cov thev naus laus zis tshawb nrhiav, nws tuaj yeem ua lub luag haujlwm tseem ceeb hauv kev saib xyuas ib puag ncig, xws li kuaj cov pa phem hauv dej.
2. Cov cuab yeej qhia ntawv thiab tshawb fawb
Hauv kev qhia thiab kev tshawb fawb, nws yog ib qho ntawm cov cuab yeej tseem ceeb. Los ntawm kev ua kom pom cov txheej txheem cov tshuaj tiv thaiv thiab cov txiaj ntsig ntawm fluorescent amines nrog cov sib xyaw sib txawv, cov tub ntxhais kawm thiab cov kws tshawb fawb tuaj yeem nkag siab zoo dua cov ntsiab lus thiab cov txheej txheem ntawm biochemistry thiab analytical chemistry.
Lub synthesis ntawmFluorescamineamines feem ntau suav nrog cov khoom siv raw xws li benzoic acid, ethylenediamine, chloroform lossis lwm yam kuab tshuaj, catalysts (xws li acids lossis bases). Qee zaum, oxidants lossis cov tshuaj txo qis kuj tseem siv tau, tab sis cov no tsis tshua muaj nyob rau hauv kev sib txuas ncaj qha ntawm fluorescent amines.
Synthesis cov kauj ruam thiab tshuaj sib npaug
Ua ntej, benzoic acid tuaj yeem ua rau muaj kev cuam tshuam nrog ethylenediamine los tsim N-phenylethylenediamine amide (ntawm no peb xav tias cov khoom lag luam nruab nrab yooj yim, vim tias cov khoom lag luam nruab nrab yuav nyuaj dua). Cov kauj ruam no feem ntau yuav tsum tau ua nyob rau hauv acidic los yog alkaline tej yam kev mob los txhawb cov tshuaj tiv thaiv ntawm carboxyl thiab amino pawg.
Kev sib npaug tshuaj (xws li cov khoom nruab nrab yog N-phenylethylenediamine amide):
Benzoic Acid + Ethylenediamine → N-Phenylethylenediamide + Dej
Lus Cim: Qhov kev sib npaug no yog ib qho piv txwv, thiab cov khoom sib txawv ntawm amide tej zaum yuav raug tsim los ntawm cov tshuaj tiv thaiv tiag tiag, yuav tsum muaj cov catalysts (xws li acids lossis bases) thiab cov tshuaj tiv thaiv tsim nyog (xws li kub thiab cov kuab tshuaj).
Cov kauj ruam tom ntej no yuav cuam tshuam txog kev cuam tshuam cyclization, uas cuam tshuam rau kev hloov ib feem ntawm cov qauv amide rau hauv cov qauv cyclic. Txawm li cas los xij, cov tshuaj tiv thaiv cyclization ncaj qha hloov N-phenylethylenediamine amide rau fluorescent amine tsis muaj vim tias cov qauv ntawm fluorescent amine yog qhov nyuaj, nrog rau cov qauv benzoxazolone. Yog li ntawd, cov kauj ruam no yuav tsum muaj kev qhia txog lwm pab pawg ua haujlwm lossis kev hloov kho cov qauv ntxiv.
Assumed cyclization cov tshuaj tiv thaiv (rau kev piav qhia nkaus xwb, tsis yog txoj hauv kev tiag tiag):
N-Phenylethylenediamide → Intermediate Cyclic Compound
Ntawm no, 'Intermediate Cyclic Compound' sawv cev rau qhov tsis tseeb ntawm qhov nruab nrab cyclic compound uas yuav tsum muaj cov tshuaj tiv thaiv ntxiv kom hloov mus rau hauv fluorescent amine.
Txhawm rau hloov cov cyclic intermediates rau hauv fluorescent amines, oxidation, halogenation, condensation, lossis lwm yam kev ua haujlwm ntawm pawg hloov pauv yuav tsum tau ua. Cov hom tshwj xeeb thiab cov xwm txheej ntawm cov tshuaj tiv thaiv no yog nyob ntawm cov qauv ntawm qhov nruab nrab thiab cov qauv ntawm cov khoom lag luam fluorescent amine.
Vim muaj txoj hauv kev sib txawv thiab sib txawv ntawm cov fluorescent amines, qhov sib npaug tshuaj tsis tuaj yeem muab rau ntawm no. Tab sis feem ntau hais lus, cov tshuaj tiv thaiv no yuav koom nrog kev siv cov oxidants (xws li hydrogen peroxide, poov tshuaj permanganate, thiab lwm yam) los qhia cov pa atoms, los yog siv cov tshuaj halogenating (xws li chlorine gas, sodium bromide, thiab lwm yam) los qhia halogen atoms, thiab ntxiv cov tshuaj tiv thaiv los tsim cov qauv ntawm fluorescent amines.
Txawm hais tias siv txoj kev sib xyaw ua ke, cov tshuaj tiv thaiv sib xyaw thaum kawg yuav tsum tau ua kom huv si kom tau txais siab -purity fluorescent amines. purification txoj kev yuav muaj xws li pom, ntxuav, ziab, crystallization, recrystallization, chromatographic sib cais, thiab lwm yam.Fluorescamine.
Lub hauv paus ntsiab lus ntawm fluorescent amine nrhiav kom tau rau cov amino pawg yog cov tshuaj tiv thaiv raws li fluorescent nrhiav tau technology, uas siv cov tshuaj tiv thaiv tshwj xeeb ntawm fluorescent amines thiab cov amino pawg (tshwj xeeb tshaj yog thawj pawg amine) los tsim cov khoom uas muaj zog fluorescence, yog li ua tiav kev tshawb pom ntawm cov amino pawg.
hom CNC machining
Cov yam ntxwv tseem ceeb ntawm fluorescent amines
Fluorescent amine yog ib qho organic compound uas muaj cov haujlwm tshwj xeeb hauv nws cov qauv molecular. Cov pab pawg ua haujlwm no tuaj yeem hloov pauv tshuaj lom neeg thaum ua haujlwm nrog cov amino pawg, tsim cov tshuaj fluorescent tshiab. Fluorescent amines lawv tus kheej tsis muaj cov fluorescence tseem ceeb nyob rau hauv ib txwm muaj, tab sis thaum lawv hnov mob nrog cov amino pawg, lawv cov khoom fluorescence tau hloov pauv tseem ceeb, tso cai rau cov khoom tsim tawm kom muaj zog fluorescence nyob rau hauv lub teeb lub teeb tshwj xeeb.
CNC tshuab dhos
Reaction mechanism ntawm fluorescent amine thiab amino pawg
Cov tshuaj tiv thaiv ntawm fluorescent amine thiab amino pawg feem ntau tshwm sim ntawm pawg carbonyl (C=O) ntawm fluorescent amine thiab nitrogen atom ntawm amino pawg. Raws li qhov tsim nyog cov tshuaj tiv thaiv (xws li tus nqi pH, qhov kub thiab txias, thiab lwm yam), cov pa roj carbonyl pawg ntawm fluorescent amine yuav raug cov tshuaj tiv thaiv condensation nrog cov amino pawg, tsim Schiff puag qauv. Cov txheej txheem tshuaj tiv thaiv no feem ntau yog nrog los ntawm kev tawg thiab tsim cov tshuaj tiv thaiv kab mob, nrog rau kev hloov hauv cov qauv hluav taws xob, ua rau muaj kev hloov pauv tseem ceeb hauv cov khoom fluorescence.
Tshwj xeeb, thaum fluorescent amine reacts nrog ib pawg amino, carbonyl oxygen atom nyob rau hauv lub fluorescent amine molecule tawm tsam cov nitrogen atom ntawm cov amino pawg, tsim ib tug tshiab carbon nitrogen ob daim ntawv cog lus (C=N). Cov tshuaj tiv thaiv no tsis tsuas yog hloov cov qauv hluav taws xob ntawm fluorescent amine molecules, tab sis kuj ua rau cov khoom tsim tawm kom muaj zog fluorescence nyob rau hauv excitation ntawm ultraviolet los yog pom lub teeb. Qhov kev siv thiab wavelength ntawm cov teeb liab fluorescent no feem ntau muaj feem xyuam rau cov concentration ntawm reactants, cov tshuaj tiv thaiv kab mob, thiab cov qauv ntawm cov khoom.
FAQ
Cov tshuaj fluorescamine nrog amine yog dab tsi?
Fluorescamine txhais tau tias yog amine -reactive dye uasCov ntaub ntawv siv fluorescent adducts thaum cov tshuaj tiv thaiv nrog cov protein thawj amines, tso cai rau staining ntawm cov protein ua ntej SDS tube gel electrophoresis. Nws nthuav tawm qhov siab tshaj plaws ntawm kwv yees li 390 nm thiab emission siab tshaj plaws ntawm kwv yees li 475 nm.
Cov amino acids twg yog fluorescent?
Peb lub fluorescent amino acids yog tryptophan (Trp), tyrosine (Tyr), thiab phenylalanine (Phe). Thaum tag nrho peb yog fluorescent, tryptophan yog qhov siv dav tshaj plaws hauv kev siv tshuaj lom neeg vim tias nws muaj cov khoom muaj zog tshaj plaws fluorescence, txawm tias nws cov wavelengths nyob rau hauv ultraviolet ntau yam.
Dab tsi yog cov tshuaj fluorescent?
Xanthene derivatives: fluorescein, rhodamine, Oregon ntsuab, eosin, thiab Texas liab.
Cyanine derivatives: cyanine, indocarbocyanine, oxacarbocyanine, thiacarbocyanine, thiab merocyanine.
Squaraine derivatives thiab nplhaib- hloov squaraines, suav nrog Seta thiab Square dyes.
Squaraine rotaxane derivatives: See Tau dyes.
fluorescence pom dab tsi?
Fluorometry yog cov txheej txheem ntawm kev ntsuas txhua qhov ntsuas uas siv fluorescent emission. Hauv cov ntsiab lus ntawm bioscience niaj hnub no, fluorometry yog siv los kuaj xyuasbio macromolecules xws li cov proteinthiab ntsuas lawv cov concentration hauv cov qauv sim.
Cim npe nrov: fluorescamine cas 38183-12-9, lwm tus neeg, manufacturers, hoobkas, lag luam wholesale, yuav, nqe, tej, kev muag khoom