Shaanxi Bloom Tech Co., Ltd. yog ib qho ntawm cov neeg tsim khoom lag luam thiab cov neeg muag khoom sai tshaj plaws 14263-94-6 nyob hauv Suav teb. Txais tos rau lag luam wholesale? Zoo zoo xiav B ntsev CAS 14263-94-6 kev muag khoom ntawm peb lub hoobkas. Cov kev pabcuam zoo thiab tus nqi tsim nyog muaj.
Ceev xiav b ntsev, nrog lub npe tshuaj 3,3 '- dimethoxy}}} paheej rabitrogen ntsev, yog lub teeb daj ua xim daj rau cov hmoov av xim av lossis siv lead ua. Nws cov yam ntxwv tseem ceeb nyob rau hauv nws cov khoom siv tsis ruaj khov, uas yog tshwj xeeb tshaj yog rhiab rau lub teeb thiab kub. Nws tuaj yeem nrawm decompose hauv nruab nrab ntawm nruab nrab lossis alkaline cov kev daws teeb meem. Cov cuab yeej tseem ceeb tshaj plaws ntawm cov compound no yog nws cov kev ua si diazotization muaj zog. Nyob rau hauv coupling cov kev tawm tsam, nws pabcuam ua lub diazo tawm tsam thiab muaj peev xwm nrawm nrawm nrog ntau lub zog, xws li xiav, ntshav, lossis dub. Yog li ntawd, nws tau siv dav hauv kev lag luam Dow, textile luam ntawv thiab dyeing thiab biochemical nrhiav kev teb. Tshwj xeeb tshaj yog nyob rau hauv cov dab neeg, nws yog siv los ua cov xim tseem ceeb substrate, rau kev tshawb nrhiav phosphatase kev ua si. Nws tuaj yeem tsim cov precoze precipate ntawm enzyme qhov chaw ua si, yog li ua tiav qhov tseeb hauv zos thiab kev ua kom pom tseeb ntawm cov khoom sib txawv lossis cov haujlwm hauv cov piv txwv.
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Tshuaj Cov Qauv |
C14h12Cl2n4O 22+ |
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Muaj pes tsawg leej |
338 |
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Molecular hnyav |
339 |
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m/z |
338 (100.0%), 340 (63.9%), 339 (15.1%), 342 |
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Kev Ntsuam Xyuas |
C, 49.58; H, 3.57; Cl, 20.90 ;90; N, 16.52; O, 9.43 |
Ceev xiav b ntsevMuaj cov ntawv thov thoob plaws hauv Biochemistry, Dow kev lag luam, thiab lwm thaj chaw. Nws siv ua protein ntau ntawm cov neeg sawv cev, cov ntaub so ntswg staining, thiab enzyme tus neeg saib xyuas, thiab kev tshawb fawb nyob rau hauv biochemical kev tshawb fawb; Raws li ob tus neeg sawv cev xim thiab cov xim tsim tus neeg sawv cev, nws muaj ntau cov kev siv hauv kev lag luam Dow thiab tshuaj ntsuam tshuaj; Tsis tas li ntawd, nws tseem muaj qee yam ntawv thov muaj nuj nqis hauv daim teb xws li kev tshawb fawb tshawb fawb thiab kuaj mob.
Biochemistry Field
1. Cov protein staining thiab electrophoreses
Nws yog feem ntau siv los ua protein staining tus neeg sawv cev hauv cov kev sim biochemical. Nws tuaj yeem khi rau cov amino acid residues nyob rau hauv cov protein, xws li Lysine, los ua cov bands pom. Nyob rau hauv cov txheej txheem ntawm sds - nplooj (sodium dulecyl sulecyl sulecyl sulfate polyacrylamide) Qhov no staining txoj kev muaj qhov siab zoo thiab feem ntau, thiab yog ib qho ntawm cov cuab yeej tseem ceeb hauv kev tshawb fawb biochemical.
2. Kev Tshawb Fawb Txog Kev Tshawb Fawb
Hauv kev tshawb fawb keeb kwm, nws tseem yog ib tus neeg sawv cev tseem ceeb. Nws tuaj yeem siv rau staining cov ntaub so ntswg, pab cov kws tshawb fawb soj ntsuam thiab txheeb xyuas cov qauv thiab kev ua haujlwm ntawm cov ntaub so ntswg. Los ntawm cov ntaub so ntswg, cov kws tshawb nrhiav tuaj yeem nkag siab cov qauv qauv, thiab faib cov ntaub so ntswg sib txawv, muab kev txhawb nqa zoo rau kev kuaj mob thiab kho cov kab mob.
3. Cell Staining
Ua si lub luag haujlwm tseem ceeb hauv kev tshawb fawb ntawm tes thiab keeb kwm histological. Nws tuaj yeem siv rau tes staining, pab cov kws tshawb fawb soj ntsuam cov qauv thiab morphology ntawm cov kab mob hlwb, thiab tom qab ntawd soj ntsuam lawv cov haujlwm thiab lub xeev. Los ntawm Cell Staining, cov kws tshawb nrhiav tuaj yeem intuitidly nkag siab cov txheej txheem ntawm kev loj hlob ntawm tes, faib, agoptosis, thiab lwm yam kev kawm ntawm kev kawm ntawm xov tooj ntawm tes thiab pathology.
4. Immunoblotting
Hauv kev sim tshuaj tiv thaiv kab mob, nws tuaj yeem siv ua cov keeb kwm yav dhau los ntawm cov neeg sawv cev. Los ntawm kev nce qhov sib piv ntawm lub teeb liab, nws tuaj yeem txhim kho qhov raug thiab rhiab ntawm kev sim. Kev txhaj tshuaj tiv thaiv kab mob yog ib txoj kev tseem ceeb rau kev ntes cov protein lus tshaj tawm, thiab nws daim ntawv thov ntxiv kom tau txais kev ntseeg tau thiab qhov tseeb ntawm hom no.
5. Enzyme ua si assay
Nws kuj tseem siv tau los ntsuas kev ua si ntawm ntau yam enzymes. Piv txwv li, nws tuaj yeem siv ua ib qho reaant lossis reagent kom koom nrog diazotizical couplings, thiab qib kev ua si ntawm cov kev hloov pauv ntawm cov xim hloov ntawm cov tshuaj tiv thaiv. Cov qauv no muaj kev tshawb xyuas dav dav hauv kev tshawb fawb biochemical thiab kuaj sojntsuam.
Nyob rau hauv qhov chaw ntawm Doz Kev Lag Luam
1. Tus neeg sawv cev xim
Raws li qhov zas xim sib txuas, nws tseem muaj cov ntawv thov tseem ceeb hauv kev zas xim. Nws tuaj yeem raug siv kom zas ntau cov fibers thiab cov ntaub ntawv pov tseg, muab cov xim ci thiab ntev} {1} kav xim ceev. Vim nws qhov kev ua tau zoo heev thiab kev ruaj khov, nws tau siv dav hauv cov ntawv luam ntawv thiab cuam tshuam kev lag luam.
2. Chromogenic Tus Neeg Sawv Cev
Hauv tshuaj ntsuam xyuas tshuaj, nws yog feem ntau siv raws li tus neeg sawv cev xim.Ceev xiav b ntsevYuav tuaj yeem yauv cov tshuaj lom neeg nrog cov khoom siv tau kuaj thiab tsim cov xim tseem ceeb hloov pauv, yog li ua tiav cov kev tsim nyog ntawm lub hom phiaj. Piv txwv li, hauv ntawv chromatography thiab nyias- txheej yog feem ntau siv los ua cov xim reagent kom pom cov tebchaw xws li flavonoids, amines, thiab phenols.
Lwm daim teb
1. Kev Tshawb Fawb Kev Tshawb Fawb
Nws tseem muaj ntau cov kev siv hauv kev tshawb fawb tshawb fawb. Txawm hais tias nws yog cov kev sim biochemical lossis lwm hom kev tshawb fawb tshawb fawb, tsuav yog lawv koom nrog kev tsim kho lossis kev xaiv xim rau cov kws sim. Nws cov kev siv thiab kev ua tau zoo tshaj plaws ua rau nws tau pom zoo hauv kev tshawb fawb science.
2. Kev kuaj mob kuaj mob
Hauv kev kuaj mob hauv kev kuaj mob, nws tseem muaj qee tus nqi thov. Txawm hais tias nws ncaj qha daim ntawv thov nyob rau hauv kev kuaj mob yog qhov tsawg dua, nws sib xyaw nrog lwm cov kab mob kuaj mob lossis cov hau kev tuaj yeem muab cov kab mob kev kuaj mob. Piv txwv li, hauv qee qhov kev xeem biochemical, nws yuav raug siv los ua cov xim reagent lossis reactant los pab txiav txim tus neeg mob cov xwm txheej thiab mob siab.
Cov synthesis ntawmCeev xiav b ntsevFeem ntau cuam tshuam ob kauj ruam tseem ceeb: Diazotization cov tshuaj tiv thaiv thiab coupling cov tshuaj tiv thaiv. Cov tshuaj tiv thaiv diazotization yog cov txheej txheem los ntawm kev hloov cov paj nyeem uas muaj cov ntsev ua yeeb yam ntawm cov khoom siv tsis zoo lossis cov txheej txheem ntawm cov yeeb yaj kiab uas muaj ntxhiab tsw los ua cov tebchaw zo.
Cov ntsiab lus piav qhia ntxaws ntxaws synthesis thiab tshuaj lom neeg sib npaug
1. Diazotization cov tshuaj tiv thaiv
Kauj ruam Qhia:
(1) Choose appropriate aromatic amines as starting materials, such as o-phenylenediamine or its derivatives.
(2) Raws li qhov kub tsawg (xws li 0-5 depuric c) thiab acidic acid) tej yam kev mob, rov qab ua yeeb yam muaj ntxhiab tsw qab nrog sodium nitrite.
(3) Los ntawm kev tswj hwm cov tshuaj tiv thaiv kub thiab cov tshuaj tiv thaiv diazotization tuaj yeem ua tiav cov tshuaj tsw qab zoo, tsis txhob muaj kev txiav txim siab ntawm Diazonium satts thiab qhov tshwm sim ntawm cov sab nraud.
Piv txwv ntawm kev sib npaug tshuaj:
Piv txwv tias orto phenylenesiamine reacts nrog sodium nitrite raws li acidic tej yam kev mob los ua cov yeeb yam, cov tshuaj tiv thaiv kab mob tuaj yeem qhia raws li:
C6h4 (NH2) {2 + 2 nano {}}}} hc} hch4 (n2cl) 29 {9o
Lus Cim: Txoj kab sib npaug saum toj saud yog qhov yooj yim sawv cev, thiab hauv cov kev sib piv txwv yooj yim, thiab ntau dua cov kev sib txuam nrog kev cuam tshuam nrog.
2. Coupling cov tshuaj tiv thaiv
Kauj ruam Qhia:
(1) Cov Diazonium tau sib txuas nrog cov molecule txuas ntxiv ntawm cov ntxhiab tsw muaj ntxhiab amine lossis cov phenolic compound raws li cov xwm txheej tsim nyog.
(2) Tswj cov tshuaj tiv thaiv kub, pH nqi, thiab lub sijhawm tiv thaiv thiab lub sijhawm tiv thaiv los ua kom cov kev kawm tau zoo ntawm kev sib tw ntawm cov khoom lag luam.
(3) Tom qab cov tshuaj tiv thaiv tiav, ntshiab thiab ruaj khov xiav bint yog tau los ntawm kev ua kom tsim nyog xws li pom, ntxuav, thiab lwm yam.
Piv txwv ntawm kev sib npaug tshuaj:
Vim tias qhov tseeb tias cov qauv tshwj xeeb thiab cov qauv khoom ntawm kev sib txuas ua ke yuav txawv nyob ntawm cov reactants, tsis tuaj yeem muab tshuaj rau ntawm no. Tab sis feem ntau hais lus cov kev tshuaj ntsuam tau tshaj tawm tuaj yeem qhia tau raws li cov txheej txheem txuas ntawm diazonium satound amine lossis sib sau ua ib qho kev sib koom tes AZO tshiab (n= n).
Ceev xiav b ntsevPuas yog siv los npaj kev kuaj cov kab mob sib koom tes kom pom kev tawm tsam, suav nrog cov kauj ruam hauv qab no:
Cov kev daws teeb meem kev sib xyaw ua ke, kev daws teeb meem tsis txaus, lub stabilizer, thiab cov neeg tsis sib haum xeeb; Dissolve lub substrate nyob rau hauv kev daws teeb meem muaj kev daws teeb meem tsis meej, stabilizer, thiab dispersant kom tau txais cov tshuaj raus; The substrate is glycyl arginine 4-methoxy - - naphthylamine hydrochloride, glycyl arginine - - naphthylamine, coumarin derivative or umbelliferone derivative; Cov kev daws teeb meem tsis muaj txiaj ntsig yog TRISY HCL yog Potassium Dihatium dihydrogen phosphate sodium hydroxide tsis, lossis boric acid bornax tsis; Lub stabilizer yog sucrose, trehalose, qab zib, lactose, galactose, galactose lossis arabinose; Tus dispersant yog ib lossis ntau dua ntawm pvp - k30, PVP-K90, PG-K90, PG-2000, thiab Peg-6000;
Cov kev daws teeb meem npaj txhij npaj thaum kauj ruam 1 yog soaked hauv cov ntawv lim uas siv lub tshuab nqus dej kom qhuav los npaj cov ntawv xeem; Qhov ntsuas kub ntawm lub tshuab ziab khaub ncaws: tom qab pib ua kom sov, teeb tsa lub tshuab kub loj rau 60-80℃thiab qhov ntsuas cua sov rau 60 degree. Thaum lub cua sov kub txog 60-80℃thiab qhov kub ntawm txhua chav kom qhuav ntau dua los yog sib npaug rau 60 degree, pib siv cov kev tov khoom; Raus nyem lub tshuab ziab khaub ncaws chaw: 7.0-11.0Hz;
Cov xim tsim cov kev daws teeb meem muaj cov xim tsim xim tus neeg sawv cev, lub stabilizer, thiab dispersant; Cov xim tsim tus neeg sawv cev, stabilizer, thiab dispersant yog yaj hauv sulphuric acid idrol lossis distilled dej los npaj cov xim tsim cov tshuaj; Cov xim tsim tus neeg sawv cev yog dimethylaminocinnamnammyde, p {- doog bb, cov xim xiav RR ntsev, kua liab t los yog cov xim liab Tr; Lub stabilizer yog tritonx - 100 lossis tritonx {5 5}} 200; Tus dispersant yog pvp-k30 lossis PVP-K90; Qhov kev daws teeb meem Colorimetric tau siv rau hauv kev sib txuas nrog cov kab txaij kom pom cov kab mob sib xyaw ua ke hauv lub taub hau.
Cim npe nrov: BATH XIAV BATH 14263-94-6, Cov chaw muag khoom, chaw tsim khoom, Hoobkas, lag luam, yuav, ntau, loj, rau muag