Shaanxi BLOOM Tech Co., Ltd. yog ib tug ntawm cov uas paub txog manufacturers thiab lwm tus neeg ntawm trypsin hmoov cas 9002-07-7 nyob rau hauv Suav teb. Txais tos rau cov lag luam wholesale bulk zoo trypsin hmoov cas 9002-07-7 muag ntawm no los ntawm peb lub hoobkas. Kev pabcuam zoo thiab tus nqi tsim nyog muaj.
Trypsin hmoovyog ib hom protease, EC 3.4.4.4, Molecular mis C35H47N7O10, CAS 9002-07-7, Nws yog ib qho serine protease muab rho tawm los ntawm pancreas ntawm nyuj, yaj, thiab npua. Hauv vertebrates, nws ua haujlwm raws li lub plab zom mov. Nyob rau hauv lub pancreas, lub precursor ntawm trypsin, trypsinogen, yog synthesized thiab secreted ua ib feem ntawm pancreatic kua txiv. Nws raug txwv los ntawm enterokinase lossis trypsin txhawm rau txhawm rau ua kom tau txais trypsin, uas yog endopeptidase uas tuaj yeem tshem tawm cov carboxyl sab ntawm lysine thiab arginine residues hauv polypeptide chains. Nws tsis tsuas yog ua haujlwm raws li lub plab zom mov, tab sis kuj txwv qhov kev puas tsuaj ntawm precursors ntawm lwm cov enzymes xws li chymotrypsin, carboxypeptidase, thiab phosphatidylcholine, yog li ua rau lawv. Nws yog qhov tshwj xeeb tshaj plaws protease thiab ib qho cuab yeej tseem ceeb hauv kev txiav txim siab cov amino acid kev npaj ntawm cov proteins.
Ib hom protease, uas yog ib qho serine protease muab rho tawm los ntawm pancreas ntawm nyuj, yaj, thiab npua, thiab ua haujlwm raws li kev zom zaub mov hauv vertebrates. Lub precursor ntawm trypsin, trypsinogen, yog synthesized thiab secreted raws li pancreatic kua txiv Cheebtsam nyob rau hauv lub txiav txiav, thiab yog tawg mus rau hauv active trypsin nyob rau hauv kev txwv ntawm enterokinase los yog trypsin. Nws yog ib qho endopeptidase uas tuaj yeem tshem tawm cov carboxyl sab ntawm lysine thiab arginine residues hauv polypeptide chains. Nws tsis tsuas yog ua haujlwm raws li lub plab zom mov, tab sis kuj txwv tsis pub decomposition ntawm precursors xws li chymotrypsin, carboxypeptidase, phospholipase, thiab lwm yam. Nws yog qhov tshwj xeeb tshaj plaws protease thiab cov cuab yeej indispensable rau kev txiav txim siab protein amino acid kev npaj.
Trypsin hmoovyog khov-qhuav qhuav tau los ntawm kev rho tawm thiab ua kom huv si los ntawm lub txiav ntawm nyuj, npua, thiab yaj. Yooj yim rau yaj hauv dej, insoluble hauv cov kuab tshuaj organic xws li chloroform, ethanol, thiab ether. Ntawm pH 1.8, luv- lub sij hawm boiling yuav luag tsis inactivate; Cov cua kub hauv cov tshuaj alkaline ua rau muaj qhov tsis zoo thiab nag lossis daus, thaum Ca2+ muaj kev tiv thaiv thiab ua kom muaj zog. Lub isoelectric point ntawm trypsin yog pH 10.1.
Bovine trypsin Ameslikas muaj 229 amino acids, muaj 6 khub ntawm disulfide bonds, thiab nws cov amino acid sequence thiab crystal qauv tau elucidated. Raws li tus kheej catalysis ntawm enterokinase kev ua si, peptide daim ntawv cog lus ntawm N-terminal lysine thiab isoleucine residues ntawm zymogen yog hydrolyzed, tso cov valin-tiantiantian-lysin 6-peptide thiab tsim cov trypsin nquag. Lub bovine trypsin muaj 223 amino acid residues thiab molecular hnyav ntawm 23800. Lub serine residue nyob rau hauv lub active site yog ib qho tseem ceeb serine protease.
Cov qauv tshuaj ntawm npua trypsin zoo ib yam li bovine trypsin. Nyob rau hauv ib nrab ntawm cov amino acid residues, tsuas yog 41 amino acid residues txawv, tab sis lub molecular configuration yog ho txawv. Kev sib haum xeeb coefficient S20W yog 2.77S, pI=10.8, Lub thermal stability yog ruaj khov dua bovine trypsin, thiab cov nyhuv tiv thaiv ntawm Ca2+ ntawm cov enzyme tsis tseem ceeb npaum li cov nyuj thiab yaj. Tsis muaj qhov chaw nruab nrab rau chelating Ca2+. Muaj 6 khub ntawm disulfide daim ntawv cog lus, thiab kev tawg 1-2 daim ntawv cog lus tsis ua kom tiav cov qauv ntawm cov enzyme molecule thiab tiv thaiv nws cov haujlwm. Kev ua haujlwm enzyme yog sib npaug rau 72% ntawm nyuj thiab 61% ntawm yaj, feem.
Yaj trypsin zoo ib yam li cov nyuj thiab npua, tab sis nws txoj haujlwm yog me ntsis siab dua li cov nyuj thiab npua.
Cov kev ua tshwj xeeb ntawm trypsin suav nrog peptide daim ntawv cog lus tsim los ntawm alkaline amino acids arginine thiab lysine carboxyl pawg. Enzymes lawv tus kheej yog yooj yim autolytic, hloov los ntawm - trypsin mus rau - trypsin, uas yog ntxiv degraded rau trypsin zoo li enzymes, thiab txawm tawg. Lawv txoj haujlwm maj mam txo qis thiab poob.
Ntxiv nrog rau muaj nyob rau hauv vertebrates, nws kuj muaj nyob rau hauv ntau yam kab mob xws li silkworms, hiav txwv chariots, barnacles, thiab actinomycetes. Tsis tas li ntawd, proteases xws li thrombin, fibrinolytic enzyme, thiab vasopressin, uas muaj feem xyuam rau cov ntshav coagulation thiab o nyob rau hauv cov tsiaj siab dua, yog ze ze rau trypsin nyob rau hauv cov nqe lus ntawm cov qauv tshuaj thiab tshwj xeeb. Nws tuaj yeem txiav txim siab tias cov enzymes no tau hloov zuj zus los ntawm cov poj koob yawm txwv enzymes. Trypsin thiab elastase kuj muaj kev sib raug zoo nyob rau hauv cov nqe lus ntawm cov qauv thiab catalytic mechanism, tab sis lawv qhov tshwj xeeb yog txawv kiag li.
Trypsin is a proteolytic enzyme extracted from the pancreas of cows, sheep, or pigs. According to Chinese drug standards, the potency of each 1mg should not be less than 2500 units calculated based on the dry product. A peptide endonuclease extracted from the pancreas of cows, sheep, and pigs, which breaks only the peptide bonds formed by the carboxyl groups of lysine or arginine. White or beige crystalline powder. Soluble in water, insoluble in ethanol, glycerol, chloroform, and ether. Molecular weight 24000, pI 10.5, The optimal pH value is around 7.8-8.5. PH>9.0 irreversible inactivation. Ca2+ muaj cov nyhuv stabilizing ntawm kev ua enzyme; Hnyav hlau ions, organic phosphorus compounds DFP, Ntuj trypsin inhibitors muaj zog inhibit nws cov haujlwm. Clinically siv los tiv thaiv - mob thiab tiv thaiv - lub hom phiaj inflammatory, thiab industrially siv nyob rau hauv tawv manufacturing, raw silk ua, ua zaub mov, thiab lwm yam.
Txoj kev npaj ntawmtrypsin hmoov:
Siv cov tweezers thiab txiab kom tshem tawm cov rog sib txuas, thiab lwm yam los ntawm cov txiav txiav tshiab, thiab muab tso rau hauv lub grinder kom zom nws, hnyav 15Kg;
Muab cov khoom minced tso rau hauv lub thoob yas, tsau nws nrog 0.125mol / L sulfuric acid tov rau 30L rau 24 teev, thiab do ib teev ib teev thaum lub sij hawm soaking;
Muab cov impregnation tso rau hauv lub hnab lim kom pom, tsau cov lim seem hauv 15L ntawm 0.125mol / L sulfuric acid tov rau 1 teev, thiab muab cov lim dej pov tseg tom qab pom;
Muab ob txoj kev daws teeb meem nrog lub ntim ntawm 44.9L, muab tso rau hauv lub thoob yas, ntxiv 242g ntawm ammonium sulfate rau txhua 1000ml ntawm cov tshuaj, ncav cuag 40% saturation, ntxiv 10.866Kg ntawm ammonium sulfate, thiab cia lawv sawv ntsug rau 12 teev;
Lim, khaws cov lim dej, qhov ntim ntawm cov lim dej yog 44L, thiab muab cov khoom pov tseg;
Ntxiv 205g ntawm cov khoom ammonium sulfate rau hauv txhua 1000ml ntawm cov kua ntshiab kom ncav cuag 70% saturation, faib 9.020kg ntawm ammonium sulfate, thiab sawv ntsug rau 12 teev;
Lim, nqa lub ncuav mog qab zib lim, hnyav 269.2g, thiab muab pov tseg cov lim;
Dissolve nrog 807.6 ml ntawm dej, thiab tom qab ntawd precipitate lub reinforcement nrog txog 40% thiab 70% saturation ntawm ammonium sulfate nyob rau hauv cov kauj ruam ntawm 2.1, 2.2, 2.3 thiab 2.4;
Siv 70% saturated sediment, hnyav 178.5g, yaj nrog 267.8ml dej, ntxiv 89.3ml saturated ammonium sulfate tov, thiab kho pH rau 5.00 nrog 5mol / L sodium hydroxide tov;
Muab cov tshuaj tso rau hauv qhov cub kub tsis tu ncua ntawm 25℃rau 48 teev, thiab yuav muaj rab koob -zoo li chymotrypsin crystals cais tawm;
Lim, kho pH ntawm niam cawv rau 3.0 nrog 0.25mol / L sulfuric acid, ntxiv 100.8g ammonium sulfate rau hauv nws ntim ntawm 328.4ml, thiab cia nws sawv ntsug rau 12 teev;
Nqa cov av thiab ziab nws hauv lub tshuab nqus tsev kom tau txais cov trypsin crude. Qhov hnyav 100.0g, uas yog, 6.67g trypsin tuaj yeem tau txais ib kilogram ntawm pancreas.
Lub cev thiab tshuaj muaj zog ntawmtrypsin hmoov:
Trypsin yog khov- qhuav qhuav ua los ntawm cov muaju muab rho tawm los ntawm cov txiav txiav ntawm nyuj, npua thiab yaj thiab purified. Yooj yim soluble hauv dej, insoluble hauv chloroform, ethanol, ether thiab lwm yam organic solvents. Ntawm pH 1.8, boiling rau ib lub sij hawm luv luv tsis yooj yim inactivates; Thaum rhuab hauv alkaline tov, denaturation precipitates, Ca2+ muaj kev tiv thaiv thiab ua kom cov nyhuv, thiab lub isoelectric point ntawm trypsin yog pH10.1.
Bovine trypsin yog tsim los ntawm 229 amino acids thiab muaj 6 khub ntawm disulfide bonds. Nws cov amino acid ib ntus thiab cov qauv siv lead ua tau qhia meej. Nyob rau hauv lub active autocatalysis ntawm enterokinase, peptide daim ntawv cog lus ntawm N-terminal lysine thiab isoleucine residues ntawm zymogen yog hydrolyzed, tso Val - hnub - hnub - hnub - hnub - hnub {{10} active procing. Bovine trypsin muaj 223 amino acid residues nrog molecular hnyav ntawm 23800. Cov serine residues nyob rau hauv lub active site yog indispensable serine.
Cov qauv tshuaj ntawm porcine trypsin zoo ib yam li bovine trypsin. Nyob rau hauv ib nrab ntawm cov amino acid residues, tsuas yog 41 amino acid residues txawv, tab sis cov molecular configuration yog txawv heev. Lub sedimentation coefficient S20W yog 2.77S, pI =10.8, thiab thermal stability yog ruaj khov dua li ntawm bovine trypsin. Cov nyhuv tiv thaiv ntawm Ca2+ ntawm enzyme tsis pom tseeb li cov nyuj thiab yaj, thiab tsis muaj qhov nruab nrab ntawm chelating Ca2+. Muaj 6 khub ntawm disulfide daim ntawv cog lus thiab 1 ~ 2 daim ntawv cog lus tawg, uas yuav tsis rhuav tshem tag nrho cov qauv ntawm cov enzyme molecule thiab tiv thaiv kev ua haujlwm ntawm cov enzyme. Kev ua haujlwm enzyme yog sib npaug rau 72% ntawm nyuj thiab 61% ntawm yaj. Yaj trypsin zoo ib yam li cov nyuj thiab npua, tab sis nws txoj haujlwm yog me ntsis siab dua li cov nyuj thiab npua.
Trypsin tshwj xeeb ua rau peptide bonds tsim los ntawm cov amino acids arginine thiab lysine carboxyl. Lub enzyme nws tus kheej yog ib qho yooj yim rau autolyse - Trypsin hloov mus rau - Trypsin yog ntxiv degraded mus rau hauv tryptase, thiab txawm tawg, thiab nws cov kev ua ub no kuj maj mam txo thiab poob.
Ntxiv nrog rau vertebrates, nws kuj muaj nyob rau hauv ntau yam kab mob, xws li silkworm, tshuaj maj, crayfish thiab actinomycetes. Tsis tas li ntawd, thrombin, fibrinolytic enzyme, vasodilator thiab lwm yam proteases muaj feem xyuam rau cov ntshav coagulation thiab o ntawm cov tsiaj siab dua yog ze ze rau trypsin nyob rau hauv cov nqe lus ntawm cov qauv tshuaj thiab tshwj xeeb. Nws tuaj yeem suav hais tias cov enzymes no txawv ntawm cov poj koob yawm txwv enzymes hauv cov txheej txheem ntawm evolution. Chymotrypsin thiab elastase kuj zoo sib xws hauv cov qauv thiab catalytic mechanism, tab sis lawv qhov tshwj xeeb yog txawv kiag li.
Trypsin is a proteolytic enzyme extracted from the pancreas of cattle, sheep or pigs. The Chinese drug standard stipulates that the potency of each 1mg of dried product shall not be less than 2500 units. An endopeptidase extracted from the pancreas of cattle, sheep and pigs, which only breaks the peptide bond formed by the carboxyl group of lysine or arginine. White or beige crystalline powder. Soluble in water, insoluble in ethanol, glycerin, chloroform and ether. The molecular weight is 24000, pI 10.5, and the optimum pH value is about 7.8~8.5. Irreversible inactivation at pH>9.0 ib. Ca2+ tuaj yeem txhim kho kev ua haujlwm enzyme; Hnyav hlau ions, organophosphorus tebchaw, DFP, thiab ntuj trypsin inhibitors muaj zog inhibit nws cov haujlwm. Nws yog siv los tiv thaiv - mob thiab detumescence hauv tsev kho mob thiab rau kev tsim cov tawv nqaij, kev ua cov ntaub qhwv nyoos thiab kev ua zaub mov hauv kev lag luam.
ua faq
trypsin yog dab tsi thiab nws txoj haujlwm?
Trypsin yogib qho enzyme uas yog qhov tseem ceeb rau koj lub cev kom zom cov protein, ib qho tseem ceeb rau kev tsim thiab kho cov ntaub so ntswg nrog rau cov pob txha, cov leeg, pob txha mos, tawv nqaij, thiab ntshav.. Thaum ua ke nrog chymotrypsin, trypsin tuaj yeem pab ua kom raug mob.
Cov tshuaj trypsin siv rau dab tsi?
Trypsin - Chymotrypsin yog ib qho tshuaj ua ke feem ntau sivkho o. Nws yog ib qho tshuaj tiv thaiv - mob thiab tshuaj antioxidant. Nws tuaj yeem txo qhov o thiab mob uas tshwm sim vim cov ntshav txhaws hauv cov ntaub so ntswg. Nws pab txo qhov mob hnyav thiab tom qab- phais o hauv qhov txhab mob.
trypsin thiab pepsin yog dab tsi?
Pepsin pib zom cov protein hauv plab. Trypsin ua tiav cov txheej txheem hauv cov hnyuv. Cov khoom kawg, cov amino acids, yog absorbed rau hauv cov ntshav thiab siv rau kev loj hlob thiab kho.
Leej twg yuav tsum tsis txhob noj trypsin?
Trypsin Chymotrypsin tsis pom zoo yog tias koj muajlub siab los yog lub raum teeb meem los yog mob ntshav siab. Koj yuav tsum qhia rau koj tus kws kho mob yog tias koj noj lwm yam tshuaj. Yog tias koj cev xeeb tub los yog pub niam mis, nrog koj tus kws kho mob tham ua ntej noj cov tshuaj no.
A: Computer Numberical Control (CNC) machining yog txheej txheem tsim khoom ua ntej - programmed computer softeare dictates lub zog ntawm fact`ory cov cuab yeej thiab machinery. Cov txheej txheem tuaj yeem siv los tswj ntau yam kev siv tshuab, los ntawm girnders thiab lathes mus rau mills thiab CNC routers.
Cim npe nrov: trypsin hmoov cas 9002-07-7, lwm tus neeg, manufacturers, hoobkas, lag luam wholesale, yuav, nqe, tej, kev muag khoom