Shaanxi BLOOM Tech Co., Ltd. yog ib qho ntawm cov tuam ntxhab thiab cov muag khoom ntawm kisspeptin-10 ntsiav tshuaj hauv Suav teb. Txais tos rau cov lag luam wholesale bulk zoo kisspeptin-10 ntsiav tshuaj muag ntawm no los ntawm peb lub hoobkas. Kev pabcuam zoo thiab tus nqi tsim nyog muaj.
Kisspeptin-10 ntsiav tshuajyog cov tshuaj tswj kev noj qab haus huv ntawm kev yug me nyuam tsim los ntawm cov tshuaj neuropeptides ntuj. Nws cov ntsiab lus tseem ceeb yog decapeptide molecule secreted los ntawm hypothalamus, uas yog tswj cov hypothalamic pituitary gonadal axis (HPG axis) los ntawm activating GPR54 receptor. Cov tshuaj no txhawb kev tso tawm ntawm luteinizing hormone (LH) thiab follicle stimulating hormone (FSH) los ntawm pituitary caj pas los ntawm stimulating pulsatile secretion ntawm gonadotropin- tso cov tshuaj hormones (GnRH), yog li tswj cov synthesis thiab tso tawm ntawm cov tshuaj hormones poj niam xws li testosterone thiab estrogen.
Cov tshuaj no qhia tau hais tias tus nqi tshwj xeeb hauv kev pabcuam kev ua menyuam yaus. Raws li qhov tshwm sim ntawm ovulation rau IVF mus, cov kev cai ntawm LH ncov tuaj yeem txo qhov kev pheej hmoo ntawm zes qe menyuam hyperstimulation syndrome (OHSS), tshwj xeeb tshaj yog haum rau cov neeg teb siab xws li polycystic zes qe menyuam syndrome (PCOS). Tsis tas li ntawd, nws txhim kho qhov ua tiav ntawm cev xeeb tub los ntawm kev tswj cov kev qhia ntawm cov noob ntsig txog endometrial receptivity, optimizing lub embryo implantation ib puag ncig.
Peb cov khoom los ntawm
Kisspeptin-10 (tib neeg) COA
Cov ntawv thov hauv kev tshawb fawb qog nqaij hlav
Kisspeptin-10 ntsiav tshuajyog ib qho tseem ceeb lom zem peptide fragment encoded los ntawm KISS1 noob. KISS1 noob yog thawj zaug pom tias yog cov qog qog nqaij hlav hauv kev tshawb fawb ntawm malignant melanoma, qhib cov lus qhia tshiab rau kev tshawb fawb txog qog noj ntshav. Cov txheej txheem tseem ceeb uas nws siv nws los tiv thaiv - cov nyhuv qog noj ntshav yog los ntawm kev khi rau G protein txuas nrog receptor GPR54 (tseem hu ua KISS1R), yog li tswj hwm lub peev xwm tsiv teb tsaws ntawm cov qog hlwb.
Hauv kev tshawb fawb txog kev mob qog noj ntshav mis, nws tau pom cov yam ntxwv tseem ceeb los tiv thaiv metastasis.
Cov kev sim tau pom tias nws tuaj yeem cuam tshuam qhov muaj peev xwm metastatic ntawm MDA-MB-435 hlwb, uas txhawb nqa kev txaus siab thiab kev tshawb nrhiav tob ntawm cov kws tshawb fawb hauv thaj tsam ntawm qog nqaij hlav metastasis.
Los ntawm hauv- kev tshuaj xyuas qhov tob ntawm theem txheej txheem, ntau- theem kev tswj hwm kev sib txuas tau tsim los ntawm kev tswj hwm ntau yam tsis yog RNAs, xws li miR-200 tsev neeg, miR-345, thiab miR-577. Hauv kev tshawb fawb ntawm glioblastoma (GBM), peptide no tau tswj hwm E-cadherin mediated intercellular adhesion los ntawm inhibiting kev qhia ntawm lub hom phiaj noob ZEB1 ntawm tsev neeg miR-200.
E-cadherin ua lub luag haujlwm tseem ceeb hauv kev sib txuas ntawm cov cellular, thiab kev saib xyuas ntawm nws cov lus qhia tuaj yeem cuam tshuam cov txheej txheem epithelial mesenchymal hloov pauv (EMT).
EMT yog txheej txheem tseem ceeb rau cov qog hlwb kom tau txais kev tsiv teb tsaws chaw thiab kev ntxeem tau, thiab inhibiting EMT tuaj yeem tiv thaiv qog nqaij hlav metastasis. Lub caij no, miR-345 downregulates qhov kev qhia ntawm anti apoptotic protein BCL2, activates lub caspase-3/8/9 cascade cov tshuaj tiv thaiv, thiab induces apoptosis nyob rau hauv cov qog hlwb. Cov ntaub ntawv sim ntxiv tau lees paub nws cov txiaj ntsig. Tom qab ua tiav, qib kev qhia ntawm EMT cov cim xws li Vimentin thiab N-cadherin hauv GBM hlwb tau txo qis dua 60%, thaum cov haujlwm cleavage ntawm Caspase-3 tau nce peb npaug, ua kom pom nws lub luag haujlwm muaj zog hauv inhibiting qog metastasis thiab inducing qog cell apoptosis.
Kev tswj cov qog microenvironment
Ua si ob lub luag haujlwm hauv kev tswj cov qog microenvironment, uas muaj ntau yam xws li qog angiogenesis thiab tiv thaiv kab mob microenvironment.
Nyob rau hauv cov nqe lus ntawm cov qog angiogenesis, ntau hom qog muaj ntau lub luag haujlwm. Nyob rau hauv cov pob txha metastasis qauv ntawm mob qog noj ntshav mis, polypeptide no tuaj yeem txhawb kev sib txawv ntawm osteoclasts thiab ua kom cov pob txha nqus cov txheej txheem los ntawm kev tswj cov kev qhia ntawm RANKL (nuclear factor kappa B receptor activator ligand). Txawm hais tias qhov txheej txheem no cuam tshuam nrog kev loj hlob ntawm cov qog nqaij hlav hauv pob txha, nws muab qhov kev xav tshiab rau kev nkag siab tob txog microenvironment ntawm cov pob txha metastasis.
Nws yog tsim nyog sau cia tias gliomas nthuav tawm cov khoom tiv thaiv angiogenic. Nws txo cov qog vascular density los ntawm 45% los ntawm inhibiting VEGF (vascular endothelial kev loj hlob yam tseem ceeb) qhia txoj hauv kev. Qhov no zoo li qhov tsis sib haum xeeb tshwm sim tuaj yeem cuam tshuam nrog qhov sib txawv ntawm cov receptors tshwj xeeb hauv ntau hom qog. Cov theem qhia thiab kev ua ntawm GPR54 receptors nyob rau saum npoo ntawm cov qog nqaij hlav sib txawv tuaj yeem sib txawv, ua rau muaj kev cuam tshuam lom neeg sib txawv.
Nyob rau hauv cov nqe lus ntawm kev tiv thaiv kab mob, kp-10 tuaj yeem cuam tshuam qhov polarization ntawm cov qog txuam nrog macrophages (TAMs).
Hauv kev kawm txog HIV ntsig txog cov kab mob neurocognitive (HAND), nws tau pom tias peptide no inhibits HIV-1 Tat induced inflammatory cytokine tso tawm los ntawm RhoA/ROCK txoj hauv kev, ua rau 50% -60% txo qis hauv cov theem ntawm inflammatory cytokines xws li IL-6 thiab TNF cov cuab yeej zoo ntawm TNF - . microenvironment, raws li cov lus teb inflammatory nyob rau hauv cov qog microenvironment feem ntau ze ze rau cov qog nqaij hlav thiab kev tiv thaiv kab mob. Los ntawm kev txo qis kev tso tawm ntawm immunosuppressive cytokines,Kisspeptin-10 ntsiav tshuajmuaj peev xwm txhim kho lub cev tiv thaiv- qog tiv thaiv kab mob, muab cov tswv yim tshiab rau kev kho mob qog noj ntshav.
Kev txhim kho ntawm kev kuaj mob biomarkers:
Raws li kev txheeb xyuas cov ntaub ntawv pov thawj, cov kws tshawb fawb tau tshawb pom qee cov noob muaj feem xyuam nrog lawv cov kev tswj hwm hauv cov neeg mob glioblastoma, xws li CDK1, CDC20, thiab lwm yam. Cov qib qhia ntawm cov noob no muaj feem cuam tshuam nrog cov kev mob tshwm sim ntawm cov neeg mob. Los ntawm kev tsim cov qauv kev kwv yees uas muaj 1401 qhov sib txawv ntawm cov noob, qhov tseeb ntawm kev kwv yees tus neeg mob muaj sia nyob tuaj yeem txhim kho mus rau 82%, muab cov ntaub ntawv tseem ceeb rau kev ntsuas kev ntsuas ntawm glioblastoma. Tsis tas li ntawd, cov ntshav concentration hauv cov neeg mob uas muaj pob txha metastases los ntawm mob qog noj ntshav mis yog 2.3 npaug ntau dua li cov neeg noj qab haus huv.
Qhov kev tshawb pom no qhia tau hais tias cov ntshav siab tuaj yeem siv los ua cov kua dej biopsy cim rau cov pob txha metastases los ntawm mob qog noj ntshav mis, muab txoj hauv kev tshiab rau kev kuaj mob ntxov ntawm cov pob txha metastases los ntawm mob qog noj ntshav.
Tshawb nrhiav cov tswv yim kho mob:
Cov phiaj xwm kev kho mob tsom rau GPR54 receptors tau dhau los ua ib qho ntawm cov hotspots hauv kev tshawb fawb kho mob qog noj ntshav tam sim no. Hauv cov qauv mob qog noj ntshav mis, ua ke nrog CDK4/6 inhibitor, nws tau pom muaj kev sib koom ua ke zoo los tiv thaiv-cov nyhuv qog. Cov txiaj ntsig ntawm kev sim tau pom tias qhov kev sib xyaw ua ke no tuaj yeem txo cov qog ntim los ntawm 75%, zoo dua li cov pab pawg monotherapy.
Cov kev tshawb fawb ntxiv tau pom tias qhov kev sib xyaw ua ke no inhibits kev loj hlob thiab metastasis ntawm cov qog hlwb los ntawm ntau txoj hauv kev los ntawm synchronously thaiv lub voj voog ntawm tes thiab EMT cov txheej txheem, yog li ua rau muaj zog tiv thaiv -cov nyhuv qog. Hauv kev kho mob ntawm glioma, cov kws tshawb fawb tau ua tiav lawv lub hom phiaj ntawm kev nkag mus rau hauv cov ntshav - lub hlwb thaiv los ntawm encapsulating nws nrog nanoparticles. Tom qab qhov kev kho mob no, qhov kev loj hlob inhibition ntawm cov qog nqaij hlav intracranial nce mus txog 68%, muab cov txiaj ntsig tshiab rau kev kho mob ntawm gliomas.
Kev siv rau hauv kev tswj hwm kev tswj hwm metabolic
Kev tswj hwm ntawm kev ua me nyuam lub zog metabolism
Nws ua lub luag haujlwm tseem ceeb hauv hypothalamic pituitary gonadal axis (HPG axis), uas tuaj yeem ua ke cov kev ua me nyuam thiab lub zog metabolism hauv lub cev thiab ua kom zoo rau kev ua me nyuam thiab lub zog sib npaug ntawm lub cev.
Hauv kev tshawb fawb nqaij qaib, cov txiaj ntsig ntawm cov peptide no ntawm kev ua me nyuam lub zog metabolism tau ua tiav tag nrho. Kev tshawb fawb tau pom tias nws tuaj yeem ua rau lub qe ntau lawm ntawm poj niam quails, thiab nws cov txheej txheem ntawm kev ua haujlwm cuam tshuam nrog kev tswj hwm ntawm cov enzymes tseem ceeb hauv daim siab lipid metabolism. Cov kev sim tau pom tias kev txhaj tshuaj tas mus li ntawm kp-10 (1 nmol / d) tuaj yeem nce qib kev qhia ntawm fatty acid synthase (FAS) hauv daim siab quail los ntawm 2.3 zaug.
AS yog ib qho tseem ceeb enzyme nyob rau hauv fatty acid synthesis, thiab ib tug nce nyob rau hauv nws cov qhia theem txhawb lub synthesis ntawm fatty acids. Nyob rau tib lub sijhawm, nws tseem txhawb nqa cov synthesis ntawm vitellogenin (VTG-II), uas muab cov khoom tsim nyog rau kev tsim cov qe los ntawm metabolic reprogramming, yog li ua kom lub qe ntau lawm ntawm quails.
Hauv cov tsiaj nyeg, muaj kev sib txawv ntawm poj niam txiv neej hauv kev tswj hwm lub zog sib npaug. Tom qab txhaj tshuaj rau txiv neej nas, kev qhia ntawm POMC (opioid melanocortin precursor) nyob rau hauv hypothalamic arcuate nucleus neurons tau upregulated.
POMC neurons ua lub luag haujlwm tseem ceeb hauv kev tswj hwm tus cwj pwm noj zaub mov, thiab kev tswj hwm ntawm lawv cov lus qhia ua rau 15% txo cov khoom noj hauv cov nas. Tom qab txhaj cov peptide rau hauv poj niam nas, kev siv nyiaj siv zog tau nce 12% los ntawm kev txhim kho thermogenic muaj nuj nqi ntawm cov ntaub so ntswg adipose. Cov txiaj ntsig tshwj xeeb ntawm poj niam txiv neej no tuaj yeem cuam tshuam txog qhov sib txawv ntawm cov tshuaj estrogen receptor (ER ) - kho cov teeb liab hloov pauv, vim tias qhov sib txawv ntawm cov tshuaj hormones thiab receptor qhia nyob rau hauv cov tsiaj ntawm cov poj niam txiv neej sib txawv ua rau muaj kev cuam tshuam metabolic ntawm kp-10.
Kev kho mob rog rog:
kp-10 muaj peev xwm thov tus nqi hauv kev kho mob rog. Los ntawm kev ua kom lub GPR54 receptor, peptide no tuaj yeem txhawb cov xim ntawm cov nqaij dawb adipose. Dawb adipose cov ntaub so ntswg yog lub luag haujlwm tseem ceeb rau kev khaws cia lub zog, thaum cov ntaub so ntswg adipose xim av muaj lub luag haujlwm ntawm kev ua kom sov thiab siv lub zog. Hauv kev noj zaub mov kom rog rog tus qauv, kev qhia theem ntawm UCP1 (uncoupling protein 1) hauv cov ntaub so ntswg subcutaneous adipose tuaj yeem nce peb zaug.
UCP1 yog cov protein tseem ceeb koom nrog hauv kev tsim cov cua sov hauv cov ntaub so ntswg adipose, thiab nws cov lus qhia ntxiv txhawb nqa oxidative zog los ntawm cov rog thaum txhim kho insulin rhiab heev, ua rau 28% txo cov ntshav qabzib ceev hauv cov nas. Kev tshawb fawb tshuab qhia tau tias txhim kho mitochondrial biosynthesis los ntawm AMPK / PGC-1 txoj hauv kev txhawb nqa lub zog metabolism hauv adipocytes, yog li ua tiav lub hom phiaj ntawm kev kho mob rog.
Reprogramming ntawm cev xeeb tub metabolism:
Kev xeeb tub yog ib qho txheej txheem tshwj xeeb ntawm lub cev uas xav kom lub cev tau txais cov txheej txheem metabolic reprogramming kom tau raws li qhov xav tau ntawm tus menyuam hauv plab. Thaum cev xeeb tub, nws cov qib tau hloov pauv hloov pauv, ua kom cov khoom noj khoom haus zoo rau tus menyuam hauv plab los ntawm kev tswj cov kev qhia ntawm placental nutrient transporters. Hauv nruab nrab cev xeeb tub, peptide no tuaj yeem ua rau muaj kev ua haujlwm ntawm lub cev A amino acid thauj los ntawm 40%. System A amino acid transporters yog lub luag hauj lwm rau thauj cov niam txiv amino acids mus rau fetus nyob rau hauv lub placenta, thiab lawv cov kev ua ub no muaj peev xwm ua tau raws li kev loj hlob sai ntawm tus me nyuam hauv plab rau cov amino acids.
Nyob rau tib lub sijhawm, kp-10 txo qis kev siv thiab tsim cov piam thaj los ntawm inhibiting kev qhia ntawm glucokinase hauv niam lub siab, txo qhov kev pheej hmoo ntawm ntshav qab zib thaum cev xeeb tub, thiab ua kom muaj kev noj qab haus huv ntawm niam thiab menyuam mos.
Zog txwv kev nyuaj siab:
Nyob rau hauv kev ntxhov siab xws li zaub mov tsis txaus thiab kev txwv tsis pub muaj zog, lub cev yuav tsum tau kho nws lub cev lub cev kom hloov mus rau ib puag ncig kev hloov pauv.
Kisspeptin-10 ntsiav tshuajplays lub luag haujlwm tseem ceeb hauv cov txheej txheem no los ntawm kev ua kom cov hypothalamic KNDy neurons, inhibiting cov haujlwm ntawm kev ua me nyuam axis, thiab ua ntej lub zog faib los tswj cov metabolism hauv basal, ua kom muaj sia nyob ntawm lub cev hauv qhov chaw hnyav. Qhov no physiological adaptation mechanism yog tshwj xeeb tshaj yog tshwm sim nyob rau hauv lub caij nyoog tsiaj yug me nyuam, xws li yaj, qhov twg cov qhia theem ntawm cov tshuaj no nyob rau hauv lub hypothalamus txo los ntawm 70% thaum lub sij hawm tsis yug me nyuam, nrog rau LH secretion inhibition thiab txo metabolic tus nqi, tso cai rau cov tsiaj kom ciaj sia lub sij hawm ntawm zaub mov tsis txaus.
Cov lus qhia kev tshawb fawb yav tom ntej
Multimodal txoj kev kho mob:
Tshawb nrhiav nws ua ke nrog cov tshuaj tiv thaiv kab mob tiv thaiv kab mob thiab tshuaj tiv thaiv kab mob metabolic. Hauv cov qauv mob qog noj ntshav, cov peptide ua ke nrog PD-1 antibody tuaj yeem ua kom tiav qhov kev tshem tawm ntawm cov qog mus rau 45%, uas yog qhov zoo dua li ib pawg tshuaj kho mob.
Dynamic saib xyuas technology:
Tsim kom muaj lub sijhawm tiag tiag - lub sijhawm saib xyuas platform raws li biosensors los taug qab lawv cov kev hloov pauv hauv lub cev. Piv txwv li, tsim cov fluorescent sojntsuam uas tuaj yeem nkag mus rau hauv cov ntshav- lub hlwb thaiv kom ua tiav hauv qhov chaw kuaj pom ntawm peptide concentration hauv glioma microenvironment, muab lub hauv paus rau kev kho tus kheej.
FAQ
Qhov sib txawv ntawm cov ntsev tuaj yeem cuam tshuam rau kev nqus dej noo thiab ntev - lub sij hawm cia ruaj khov, tab sis txawm tias hom ntsev, ib zaug raug rau cov dej hauv ib puag ncig (xws li cov kab dej noo hauv cov ntsiav tshuaj excipients), tus nqi degradation yuav sai sai.
Muaj ob hom ntsev tseem ceeb ntawm Kisspeptin-10 muaj nyob hauv khw:
TFA ntsev: molecular hnyav 1416.46, DMSO solubility 80 mg/mL
Acetate: CAS tus lej yog tib yam li lub hauv paus dawb (374675-21-5), kev cia khoom zoo ib yam li TFA ntsev
Ob qho tib si khov- cov hmoov qhuav tuaj yeem khaws cia ruaj khov rau 3 xyoos ntawm -20 ° C thiab 2 xyoos ntawm 4 ° C. Tab sis qhov sib txawv tseem ceeb yog tias TFA ntsev tau qis dua hygroscopicity thiab nyiam dua nyob rau hauv qee qhov xwm txheej.
Kisspeptin-10 yog qhov ruaj khov hauv lub xeev ruaj khov (tuaj yeem khaws cia hauv chav sov li 3 lub lis piam). Txawm li cas los xij, cov khoom siv hauv ntsiav tshuaj uas muaj dej tuaj yeem cuam tshuam lawv qhov khov - lub xeev tiv thaiv kom qhuav, txo qhov degradation ntawm "hli" mus rau "txhua hnub". Yog li ntawd, yog tias cov ntsiav tshuaj tau tsim tiag tiag, cov khoom siv tsis muaj dej thiab cov txheej txheem qhuav qhuav yuav tsum tau siv - tsuas yog ib tee dej yuav ua rau tag nrho cov ntsiav tshuaj tsis muaj txiaj ntsig.
Chav tsev kub stability ntawm hmoov yog txwv rau kaw, qhuav, thiab tsaus ntuj, thiab hygroscopicity ntawm cov ntsiav tshuaj excipients yuav hloov tag nrho cov cai no.
Cov hmoov qhuav qhuav tuaj yeem ruaj khov rau 3 lub lis piam ntawm chav tsev kub, uas yog lub hauv paus theoretical rau nws lub peev xwm thauj khoom ntawm chav tsev kub. Tab sis qhov xwm txheej nrog cov ntsiav tshuaj yog txawv kiag li:
Hmoov: tuaj yeem khaws cia rau 3 xyoos ntawm -20 ° C, 2 xyoos ntawm 4 ° C, thiab 6 lub hlis ntawm -80 ° C rau kev daws
Kev pheej hmoo ntawm cov ntsiav tshuaj: Yog tias cov khoom noj muaj hygroscopicity, lawv yuav maj mam nqus dej los ntawm huab cua. Thaum lub microenvironment sab hauv ntawm cov ntsiav tshuaj mus txog qhov tseem ceeb ntawm cov av noo, lub khov- lub xeev qhuav yog hloov los ntawm "lub xeev daws", thiab degradation yuav sai sai.
Ib lub ntsiav tshuaj zoo li "khoom" yuav muaj cov av noo ntau dua hauv nws cov microenvironment sab hauv dua li cov av noo ib puag ncig uas pom ntawm lub ntim sab nrauv. Yog vim li cas thiaj li yuav tsum tau khaws cia rau Kisspeptin -10 hmoov yog "qhuav thiab kaw", thaum cov ntsiav tshuaj muaj nyob, yuav tsum siv ob txoj kev tiv thaiv ntawm txhuas-yas hlwv thiab desiccant.
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