Ergosterol Hmoov CAS 57-87-4

(1) Ua kom muaj kev ncaj ncees ntawm cell membrane qauv
Raws li ib feem tseem ceeb ntawm cov kab mob fungal, cov txheej txheem membrane ruaj khov yog tsim los ntawm embedding phospholipid bilayers. Cov qauv ntawm lub voj voog nruj thiab tus Tsov tus tw hydrophobic hauv nws cov qauv molecular cuam tshuam nrog fatty acid chains ntawm phospholipid molecules, txhim kho lub zog txhua yam ntawm daim nyias nyias. Hauv cov kab mob xws li cov poov xab thiab pwm, cov tshuaj no suav nrog 30% -50% ntawm tag nrho cov lipids hauv cell membrane. Qhov kev faib ua feem siab no ua kom muaj kev ruaj ntseg ntawm cov cell membrane nyob rau hauv ib puag ncig kev ntxhov siab xws li osmotic siab hloov thiab kev siv tshuab siab.

(2) Membrane permeability tswj
Los ntawm kev kho cov fluidity ntawm daim nyias nyias, nws cuam tshuam rau kev ua haujlwm ntawm cov khoom thauj khoom thauj. Cov kev hloov pauv hauv nws cov ntsiab lus ncaj qha cuam tshuam rau kev sib haum xeeb thiab kev ua haujlwm ntawm daim nyias nyias cov protein, piv txwv li, hauv cov txheej txheem ntawm kev nqus cov khoom noj, nws tuaj yeem tswj cov kev hloov pauv ntawm cov piam thaj thauj mus los thiab txhawb nqa cov transmembrane thauj cov piam thaj. Kev sim cov ntaub ntawv qhia tau hais tias thaum nws cov ntsiab lus txo los ntawm 20%, qhov nqus ntawm cov piam thaj los ntawm brewing poov xab txo los ntawm 35%.

(3) Kev saib xyuas ntawm daim nyias nyias - khi enzyme kev ua haujlwm
Raws li qhov chaw thauj tog rau nkoj rau daim nyias nyias - khi enzymes, enzymes tswj nws cov kev ua haujlwm zoo los ntawm kev cuam tshuam tsis zoo thiab kev cuam tshuam ntawm electrostatic. Piv txwv li, nyob rau hauv cov kab mob ua pa nyuaj IV, ergosterol khi rau subunits ntawm cytochrome c oxidase los xyuas kom meej lub continuation ntawm electron thauj saw. Kev tshawb fawb pom tau hais tias nyob rau hauv mutant hom tsis muaj ergosterol, cov kev ua ntawm ua pa saw complex IV txo los ntawm 60%, thiab cov efficiency ntawm ATP synthesis txo los ntawm 45%.

(4) Cellular thauj khoom
Koom nrog hauv kev thauj mus los hauv vesicle thiab phagocytosis. Nyob rau hauv txoj kev poov xab secretion, ergosterol txhawb kev tsim thiab thauj ntawm secretory vesicles los ntawm kev tswj cov curvature ntawm Golgi apparatus membrane. Fluorescence labeling thwmsim pom tias tus naj npawb ntawm secretory vesicles nyob rau hauv ergosterol synthesis deficiency strains txo los ntawm 40%, thiab kev thauj ceev txo los ntawm 50%.

(1) Antifungal tshuaj hom phiaj
Nws yog lub hom phiaj tseem ceeb ntawm cov tshuaj tiv thaiv kab mob. Polyene tshuaj tua kab mob (xws li amphotericin B) khi nrog ergosterol los tsim cov kab mob hauv lub cev, ua rau cov dej ntws tawm ntawm cov cellular; Azole antifungal tshuaj (xws li ketoconazole) inhibit qhov tseem ceeb enzyme lanosterol 14 - demethylase (CYP51) nyob rau hauv ergosterol synthesis pathway, thaiv ergosterol synthesis. Cov ntaub ntawv kho mob qhia tau tias MIC tus nqi ntawm amphotericin B tiv thaiv Candida tuaj yeem ncav cuag 0.1-0.5 μ g / mL, thaum MIC tus nqi ntawm cov tshuaj azole tawm tsam Aspergillus yog nyob rau hauv thaj tsam ntawm 0.5-2 μ g / mL.

(2) Raw khoom rau vitamin D2 ntau lawm
Tom qab irradiation nrog lub teeb ultraviolet (280-320nm), nws lub nplhaib B tau dhau los ua cov vitamin D2 (ergocalciferol). Cov txheej txheem no cuam tshuam nrog kev cuam tshuam ntawm photolysis thiab isomerization, thiab kev hloov pauv ntawm cov khoom kawg vitamin D2 tuaj yeem ncav cuag 60% -70%. Hauv kev lag luam tshuaj, vitamin D2 yog siv los tiv thaiv thiab kho rickets thiab osteoporosis, nrog kev pom zoo noj txhua hnub ntawm 400-800IU.

(3) Hormonal tshuaj intermediates
Nws yog ib qho tseem ceeb precursor rau synthesis ntawm steroid hormones. Nws tuaj yeem hloov mus rau hauv cov tshuaj hormones xws li progesterone thiab hydrocortisone los ntawm kev hloov tshuaj. Noj progesterone synthesis ua piv txwv, ergosterol tau dhau 17 cov tshuaj tiv thaiv enzymatic, ntawm cov kauj ruam tseem ceeb suav nrog kev rhuav tshem C20-C22 daim ntawv cog lus thiab hydroxylation ntawm C17 txoj haujlwm. Cov tshuaj hormones no muaj kev siv dav hauv kev ua haujlwm xws li kev tsim cov menyuam yaus thiab kev kho mob tiv thaiv kab mob.

(1) Kev txheeb xyuas cov kab mob fungal
Cov ntsiab lus ntawm ergosterol yog qhov zoo sib xws nrog fungal biomass (R ²=0.98). Cov fungal biomass tuaj yeem suav tau los ntawm kev ntsuas cov ntsiab lus ergosterol hauv cov qauv siv siab -kev ua haujlwm ua kua chromatography (HPLC). Txoj kev no muaj qhov rhiab heev (kev kuaj pom qhov txwv ntawm 0.1 μ g / g) thiab zoo rov ua dua (RSD<5%), and has been widely used in fields such as food microbiological detection and environmental fungal contamination assessment.

(2) Kev faib tawm thiab kev txheeb xyuas cov kab mob fungal
Muaj qhov sib txawv tseem ceeb hauv ergosterol cov ntsiab lus ntawm cov kab mob fungal thiab hom. Piv txwv li, cov poov xab muaj cov ntsiab lus ergosterol ntawm 2-5mg / g qhuav hnyav, thaum Aspergillus tuaj yeem ncav cuag 8-12mg / g qhuav hnyav. Los ntawm kev sib txuas cov roj chromatography-mass spectrometry (GC-MS) thev naus laus zis los tshuaj xyuas cov qauv sib txawv ntawm ergosterol sab chains, kev txheeb xyuas meej ntawm cov kab mob fungal thiab hom tuaj yeem ua tiav.

(3) Kab mob ntsuas ntsuas
Cov metabolism tsis zoo ntawm ergosterol yog txuam nrog ntau yam kab mob. Hauv cov ntshav ntawm cov neeg mob uas muaj kab mob fungal, cov ntsiab lus ntawm ergosterol derivatives yog nce ntxiv (3-5 npaug ntau dua li cov neeg noj qab haus huv), uas tuaj yeem siv los ua cov cim qhia ntxov rau cov kab mob fungal. Tsis tas li ntawd, cov qib ntawm ergosterol oxidation cov khoom (xws li 24,25-dihydroergosterol) yog correlated nrog oxidative kev nyuaj siab thiab yuav siv tau los ntsuas qhov℃ntawm cell puas.

(1) Kev cai cog qoob loo
Exogenous ntxiv ntawm ergosterol tuaj yeem txhawb cov nroj tsuag kev loj hlob thiab kev loj hlob. Hauv kev sim kho cov noob nplej, soaking cov noob hauv 0.1mg / L ergosterol tov tuaj yeem nce germination tus nqi los ntawm 15% thiab yub qhov siab los ntawm 20%. Nws cov txheej txheem ntawm kev ua haujlwm suav nrog kev tswj hwm cov tshuaj endogenous tshuav nyiaj li cas, txhim kho photosynthetic efficiency, thiab lwm yam.

(2) Kev tsim tshuaj tua kab mob lom
Inhibitors ntawm ergosterol synthesis, xws li tebuconazole thiab tebuconazole, exert bactericidal los ntawm inhibiting fungal ergosterol synthesis. Hom kab mob fungicide no muaj txiaj ntsig zoo rau cov kab mob fungal xws li powdery mildew thiab xeb. Kev sim teb tau pom tias cov kev tswj xyuas tuaj yeem ncav cuag 80% -90%, thiab cov tshuaj lom rau cov kab mob uas tsis yog hom phiaj tsawg.

(3) Microbial fertilizer txhim khu kev ua tau zoo
Ntxivergosterol hmoovtuaj yeem txhim kho kev ruaj ntseg ntawm cov kab mob chiv formulations. Ntxiv 0.5% ergosterol rau rhizobium npaj tuaj yeem ua rau cov kab mob ciaj sia los ntawm 30% thiab nitrogen fixation efficiency los ntawm 25%. Nws cov txheej txheem ntawm kev ua haujlwm suav nrog kev tiv thaiv cell membrane kev ncaj ncees thiab txhim kho ib puag ncig adaptability.

(1) Antioxidant nyhuv
Ergosterol muaj peev xwm tshem tawm cov dawb radicals. Kev sim hauv vitro tau pom tias ergosterol muaj IC50 tus nqi ntawm 12 μ g / mL rau DPPH radicals thiab qhov tshem tawm ntawm 75% rau hydroxyl radicals (ntawm qhov concentration ntawm 50 μ g / mL). Nws cov tshuaj tiv thaiv antioxidant suav nrog ncaj qha quenching dawb radicals, chelation ntawm hlau ions, thiab lwm yam.

(2) Kev tiv thaiv kab mob
Ergosterol tuaj yeem txhim kho lub cev tiv thaiv kab mob. Cov kev sim tsiaj tau pom tias noj cov nas nrog cov zaub mov uas muaj 0.05% ergosterol nce macrophage phagocytic Performance index los ntawm 40% thiab lymphocyte proliferation rate los ntawm 35%. Nws cov txheej txheem ntawm kev ua haujlwm suav nrog kev ua kom lub TLR4 teeb liab txoj hauv kev thiab txhawb nqa cytokine secretion.

(3) Kev tiv thaiv kab mob plawv
Nws muaj lub luag haujlwm ntawm kev tswj cov ntshav lipids. Cov kev tshawb fawb soj ntsuam tau pom tias kev noj tshuaj txhua hnub ntawm 200mg ergosterol tuaj yeem txo LDL-C qib los ntawm 15% thiab nce HDL-C qib los ntawm 10%. Nws cov lipid-txo cov txheej txheem suav nrog inhibiting kev nqus cov cholesterol thiab txhawb cov kua tsib kua qaub tawm.