Hygromycin b, molecular cov mis c20h37n3o13, CAS682 {{{} 04-9, yog cov tshuaj tua kab mob hygroscopicus thiab belongs rau tsev neeg aminoglycoscoside. Nws feem ntau muaj nyob rau hauv daim ntawv ntawm ib nyuag daj xim av hmoov, uas yuav sib txawv me ntsis vim yog cov txheej txheem ntau lawm thiab kev coj dawb huv. Nws cov hmoov hauv lub xeev yog muag heev, tsis pom tseeb qhov hnov, haum rau ntau yam kev sim biochemical thiab kws tshuaj etectical. Nws yog soluble hauv methanol, ethanol, thiab dej, thiab cov polrolents no tso cai rau cov pab pawg polar hauv cov molecule, yog li nce nws solubility. Txawm li cas los xij, nws yuav luag tsis tau muaj insoluble nyob rau hauv cov kuab tshuaj nrog qis polarity xws li ether, los, kev cuam tshuam cov qauv polarity ntawm nws cov qauv molecular. Raws li lub hauv paus tsis muaj zog, nws tuaj yeem cuam tshuam nrog ntau cov organic thiab inorganic acids los tsim cov ntsev ua ke. Cov cuab yeej siv kua hluav taws xob no ua rau nws siv tau dav hauv vivo thiab hauv solubility, thiab kev ua si tuaj yeem tswj hwm los ntawm kev kho tus nqi ntawm kev daws teeb meem. Hauv chav kuaj, nws yog siv los xaiv thiab tswj cov nplooj prokaryotic thiab eukaryotic hlwb nrog hygromycin tsis kam noob. Lub Hygromycin Kuj yog Gene Encodes Kinase (Hygromycin Kinase) uas yog inactivated los ntawm phosphorylation. Txij thaum pom pom ntawm cov noob tawm tsam rau hygromycin, nws tau siv ua qauv tshuaj ntsuam hauv kev sim transgenic. Ntawm tam sim no, cov tshuaj no feem ntau siv rau kev soj ntsuam hauv cov ntaub so ntswg cog qoob loo.
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Tshuaj Cov Qauv |
C20H37N3O13 |
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Muaj pes tsawg leej |
527 |
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Molecular hnyav |
528 |
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m/z |
527 (100.0%), 528 (21.6%), 529 (2.5%), 529 (2.2%), 528 (1.1%) |
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Kev Ntsuam Xyuas |
C, 45.54; H, 7.07; N, 7.97; O, 39.43 |
Hygromycin byog cov tshuaj tiv thaiv tsim los ntawm Streptomycopicus thiab belongs rau tsev neeg aminoglycoscoside. Nws cov haujlwm ntau thiab cov chaw thov ua nws cov cuab yeej tseem ceeb hauv kev tshawb fawb thiab kev lag luam kws tshuaj.
1. Cov tshuaj tiv thaiv thiab cov tshuaj tiv thaiv kab mob
Inhibition ntawm protein synthesis:
Lub tsev loj ntawm kev nqis tes yog tua cov kab mob, fungi, thiab qee cov tsiaj txhu eukylic ntau dua los ntawm kev txwv cov protein biosynthesis hauv lawv cov hlwb. Nws tso cov trna binding site ntawm cov loj submunit ntawm tus lej loj, tiv thaiv kev txiav tawm ntawm cov tav thiab yog li inhibiting qhov txuas ntxiv ntawm kev txhais lus. Lub Inhibitt inhibitions nyhuv yog qhov ua tau zoo rau ribosomes hauv ob qho propokaryotic thiab eukaryotic hlwb.
Cov tshuaj tua kab mob dav dav:
Nws muaj ntau yam ntawm cov haujlwm tshuaj tua kab mob tiv thaiv cov kab mob ntau yam, fungi, thiab siab dua ezaryotic hlwb. Qhov no ua rau nws muaj txiaj ntsig zoo raws li tus neeg sawv cev antibacterial hauv kev ua liaj ua teb, tsiaj cov txiv, thiab cov tsiaj muaj kab mob rau cov kab mob no.
Antiviral nyhuv:
Ntxiv rau ib qho ntxiv rau cov tshuaj tua kab mob thiab cov tshuaj tiv thaiv kab mob, nws tseem muaj qee yam kev ua yeeb yam. Nws tuaj yeem xaiv cov hlwb uas ua rau muaj kev sib tw vim muaj kis tus kab mob ntawm tus kab mob thiab tsim txom nws cov kev rov ua dua tshiab thiab kis tau.
2. Daim ntawv thov hauv Transgenic thieriments
Txoj kev ntsuas qauv:
Txij li qhov pom ntawm cov noob tawm tsam rau hygromycin, nws tau siv dav siv raws li kev kuaj mob hauv kev sim transgenic. Nws tso cai rau cov kws tshawb nrhiav xaiv thiab tswj cov nplooj prokaryotic thiab eukaryotic hlwb uas muaj lub noob hygromycin kuj yog noob. Txoj kev ntsuas ntsuas no muaj cov yam ntxwv ntawm kev ua haujlwm siab, kev ntseeg tau, thiab yooj yim ntawm kev ua haujlwm.
Xaiv cov hlwb hloov pauv hloov pauv
Hauv kev sim Transenic, feem ntau nws yog feem ntau siv los ua tus xaiv los tshuaj ntsuam cov hlwb uas tau hloov ua tiav thiab qhia txog kev hygromycin kuj yog noob. Cov hlwb no tuaj yeem loj hlob thiab cov me dua hauv cov khoom nruab nrab uas muaj cov tshuaj, thaum lub hlwb tsis xwm yeem yuav raug tua.
Lim cim npe:
Hauv cov caj ces engineering thiab kev tshawb fawb Biology Cell, feem ntau nws yog feem ntau siv los ua qhov ntsuas ntsuas ntsuas. Los ntawm kev qhia cov plasmids nqa cov noob hygromycin Kuj muaj sia nyob rau hauv cov hom tshuaj lom neeg muaj peev xwm ua tiav cov khoom lag luam, yog li ua tiav kev tshuaj ntsuam thiab lub hom phiaj ntawm lub hom phiaj.
Kev coj noj coj thiab ntsuas:
Nws kuj tseem siv tau rau kev tshuaj ntsuam ntawm tes thiab ua kom huv hauv kev sim ntawm tes kab lis kev cai. Los ntawm kev kho nws cov kab kev xav tau, cov cuab yeej siv xov tooj ntawm tes uas qhia tau tias lub hom phiaj yuav raug tshuaj xyuas, muab cov hauv paus ntawm cov hlwb los qhia txog kev sim ua tom ntej rau kev tshawb fawb tom ntej rau kev tshawb fawb txuas ntxiv. Txoj kev no tsis yog tsuas yog ua tau zoo thiab txhim khu kev qha, tab sis kuj yooj yim ua haujlwm, thiab tau dhau los ua ib qho ntawm cov qauv tshuaj ntsuam ntawm kev ua lag luam genetic engineering.
3. Tshuaj Kev Txhim Kho thiab Kev Thov
Tshuaj pleev cov kab:
Muaj ntau cov kev siv hauv cov kws kho tsiaj teb. Lub sij hawm ntev noj yuav muaj peev xwm tswj tau zoo ntawm tus npua roundworms, esophageal nematodes, thiab plaub hau nematodes. Qhov no yog vim tias nws tsis yog ua tau zoo rau cov neeg laus thiab cov kab menyuam yaus, tab sis kuj tseem txwv tsis pub poj niam oviposition, uas ua rau muaj kev ua me me muaj peev xwm ntawm cov kab lub cev. Yog li no, ntxiv cov tshuaj no rau tag nrho cov khoom noj ntawm cev xeeb tub tuaj yeem tiv thaiv cov kab mob pastworm los ntawm cov kab mob kab mob thaum lactation.
Tshuaj Kho Mob:
Kuj tseem siv tau los kho kab mob los ntawm cov kab mob rhiab. Txawm hais tias nws daim ntawv thov kev kho mob tsawg kuj yog txwv tsis pub tsawg, nws tuaj yeem ua lwm txoj kev xaiv rau kev kho tus mob refractory nyob rau qee yam xwm txheej.
4. Kev ntsuas tshawb nrhiav cov cuab yeej tshawb nrhiav
Kev tshawb fawb txog cov khoom siv protein:
Nws tau dhau los ua ib qho cuab yeej tseem ceeb rau kev kawm cov khoom muaj protein vim nws txawv txav ntawm cov protein cov protein. Cov kws tshawb nrhiav tuaj yeem siv nws los tshawb txog cov kev tswj hwm cov txheej txheem ntawm protein synthesis, cov qauv ntawm ribosomes, thiab ntau yam molecular txheej xwm hauv kev txhais lus.
Kev Tshawb Fawb Txog Cell:
Nws kuj tseem siv tau rau kev kuaj mob tes thiab ua kom huv hauv kev tshawb fawb Biology Cell. Los ntawm xaiv tua neeg tsis muaj kev tua neeg lossis cov xov tooj ntawm cov hlwb, cov kws tshawb nrhiav tuaj yeem tau txais cov hom phiaj ua kom huv hauv kev tshawb fawb ntawm kev tshawb fawb ntawm cov neeg paub ntxiv.
5. Kev Tshawb Fawb Biology Cell
Kawm cov xov tooj ntawm tes
Dav siv rau kev kawm cov txheej txheem biological xws li apoptosis, teeb liab kev hloov chaw, thiab voj voog ntawm tes. Nws cov cim kev ua kom nws ua rau nws ib qho ntawm cov cuab yeej tseem ceeb rau kev kawm cov txheej txheem biological biological.
Los ntawm kev soj ntsuam thiab tshuaj ntsuam xyuas cov kev hloov cell tom qab kev kho nroghygromycin b, Peb tuaj yeem nce kev nkag siab tob txog cov molecular txheej txheem thiab cov phiajcim txoj hauv kev kuaj mob ntawm tes, muab cov tswv yim thiab cov qauv tshiab rau kev kho mob kab mob thiab kev kho yeeb tshuaj.
Cov antiviral kev tshawb fawb:
Nws tseem muaj cov tshuaj tua kab mob thiab tuaj yeem xaiv cov hlwb uas ua rau muaj kev sib kis vim yog kis tus kabmob ntawm tus kab mob thiab yog li tsim txom nws cov kev rov ua dua tshiab thiab kis tau. Qhov no ua rau nws muaj cov ntawv thov yuav muaj peev xwm thov cov txiaj ntsig kev tshawb fawb.
Cov tshuaj pleev cov kab:
Nws kuj tseem tuaj yeem sib xyaw ua ke rau hauv cov tsiaj pub kom muaj cov kev cuam tshuam kev ua haujlwm, thiab muaj txiaj ntsig zoo ntawm cov cab kuj, esophageal nematodes, thiab plaub hau nematodes.
6. Kev Siv Hluav Taws Xob
Ua ke siv nrog lwm cov tshuaj tua kab mob:
Vim tias qhov sib txawv ntawm hom kev ua ntawm Hygromycn b sib piv rau lwm cov tshuaj tua kab mob xws li G418, ZeastIDIN S, nws feem ntau yog siv rau kev xaiv cov kab cell tiv thaiv cov xov tooj ntawm tes zoo. Los ntawm kev sib txuas nrog cov tshuaj tua kab mob no, qhov ruaj khov thiab qhov tseeb ntawm kev tshuaj ntsuam tuaj yeem txhim kho ntxiv.
Kev Ntsuam Xyuas thiab Tswj cov prokaryotic lossis eukaryotic hlwb muaj hygromycin tsis kam noob:
Vim nws muaj peev xwm ua rau muaj protein synein, Hygromycn B tuaj yeem siv los ua cov cim kev tshawb fawb thiab cov xov tooj ntawm cov kab ke ua tiav tau yooj yim nrog cov noob. Cov hlwb no, nqa cov noob hlav tsis kam rau hygromycin (xws li cov khoom siv HPROMYRYRATE, uas yog muaj peev xwm ua haujlwm phosphorerase, yog li ua si detoxifying lub luag haujlwm.
Thaum kev soj ntsuam ruaj khov hloov cov hlwb, nws yog qhov tsim nyog los tsim kom muaj kev tua nkhaus los txiav txim siab qhov ntau ntawm cov tshuaj tua kab mob uas tuaj yeem tua tsis tau muaj tus tswv tsev. Qhov no feem ntau yog ib txwm ua tiav los ntawm kev xaiv ntau cov kev teeb tsa tau thiab saib cov ciaj sia ntawm cov hlwb ntawm ntau lub siab.
Raws li ib qho tseem ceeb tshuaj tua kab mob, keeb kwm ntawm hygromycin tuaj yeem raug rov qab mus rau nruab nrab-20th caug xyoo.
Lub sijhawm tshawb pom lub sijhawm:
Nws tau pom thawj zaug xyoo 1950 thiab yog metabolized los ntawm Streptomycopicus. Qhov kev tshawb pom qhov kev tshawb pom no tau pib ntawm lwm tus neeg tseem ceeb ntawm AminoglycosSide tsev neeg.
Siv ntxov:
Nyob rau hauv nws cov theem thaum ntxov ntawm kev tshawb pom, nws yog tsuas yog siv los ua cov tshuaj tsiaj thiab ntxiv rau cov qaib thiab npua, ua lub luag haujlwm ntawm cov quav thiab cov kab tiv thaiv. Daim ntawv thov no qhia txog kev tua ntawm Hygromycin ntawm cab hauv cov tsiaj.
Tshawb nrhiav ntawm cov noob:
Thaum ntxov xyoo 1980s, cov kws tshawb fawb nrhiav pom muaj cov noob tsis kam. Qhov kev pom no yog lub hauv paus rau daim ntawv thov ntawm Hygromycin hauv cov kev tshawb fawb caj ces engineering thiab kev tshawb fawb ntawm biology ntawm biology.
Chaomycin tuaj yeem txwv kev loj hlob microbial los ntawm kev tsim txom cov protein protein thiab muaj cov kev cuam tshuam dav dav. Nws muaj cov txiaj ntsig zoo muaj zog ntawm gram cov kab mob zoo, kis tus kab mob, gram cov kab mob tsis zoo, thiab lwm yam kab mob tsis zoo. Raws li dav {- ecromycin tau siv dav hauv kev tshawb fawb, tshuaj, cov kws kho tsiaj, tshuaj tua kab thiab lwm thaj teb. Chaomycin tuaj yeem tiv thaiv cov laus ovulation thiab tiv thaiv kev loj hlob ntawm txoj kev loj hlob. Ntxiv nws rau npua thiab nqaij qaib pub tuaj yeem tswj tau zoo xws li npua roundworms thiab esophageal nematodes.
Raws li daim ntawv qhia txog kev kwv yees ntawm cov khoom thiab thov kev lag luam thiab kev tsim kho ntawm Hygromycin tau nthuav tawm hauv Suav teb, Is Nrias teb, Tebchaws Europe thiab Tebchaws Asmeskas thoob ntiaj teb. Tuam Tshoj yog ib lub teb chaws loj tsiaj nyob hauv lub ntiaj teb, thiab kev ua lag luam xav tau hygromycin yog qhov dav. Tab sis nrog cov tshuaj tiv thaiv nruj los ntawm tsoomfwv thoob ntiaj teb, daim ntawv thov ntawm cov kev txwv, thiab chaw ua lag luam yuav txo qis hauv lub neej yav tom ntej.
Tus nqi lag luam ntawm hygromycin yog cuam tshuam los ntawm ntau yam khoom siv los ntawm kev sib raug zoo, thiab lwm yam kev sib raug zoo, Hunan Huibaishi Biotechnology, Huazhong Heinology (Beijing) Gene Tshuab, thiab lwm yam. Kev sib tw ua lag luam yog tsiv heev. Kev lag luam hauv tsev tuaj yeem txo cov nqi ntau lawm los ntawm kev tswj hwm kev lag luam thiab kev thauj mus los nrog cov nqi tsheb thauj khoom ntawm hygromycin feem ntau yog qis dua.
Cov tshuaj tiv thaiv cov kev mob ntawm hygromycin yog hnyav thiab synthesis cov txheej txheem yog qhov nyuaj. Cov txheej txheem synthesis muaj cov kauj ruam xws li kev sib cais, fermentation kab lis kev cai, kev sib cais, huv, thiab kom qhuav. Cov nqi ntau lawm ntawm hygromycin yog cov kev lag luam siab, thiab zoo meej lawv cov txheej txheem ntau lawm los txhim kho kev ua haujlwm ntawm hygromycin ntau lawm.
Kev siv thev naus laus zis thev naus laus zis yog ib qho tseem ceeb tshaj plaws uas tsav kev txhim kho kev ua lag luam hygromycin. Nrog rau qhov tob tob ntawm kev tshawb fawb thiab kev nce qib ntawm thev naus laus zis, cov txheej txheem ntau lawm ntawm hygromycin tau tas li tau txhim kho thiab ua tiav. Tshav Yuhui pab neeg hauv tsev muag tshuaj ntawm lub tsev muag tshuaj ntawm lub tsev kawm ntawv kab mob biosynthetic raws li cov thev naus laus zis txhim kho, muab kev txhawb nqa kev lag luam kev lag luamhygromycin bCov. Kev Lag Luam Kev Lag Luam los ntawm XinsiJie tau hais tias Hygromycin muaj broad {1} Kev tshawb fawb dav dav, cov tsiaj cov tsiaj, ua liaj ua teb, thiab tshuaj. Txawm li cas los xij, cov txheej txheem sib xyaw ua ke ntawm Hygromycin thiab kev siv ntau dhau tuaj yeem ua rau cov tshuaj tiv thaiv tsis kam, uas txo nws daim ntawv thov nyob rau qee lub sijhawm. Kev sib tw hauv kev ua lag luam hygromycin yog tsiv heev. Nrog kev txhim kho ntawm cov khoom siv hluavtaws, Hygromycin kev lag luam yuav tsum txais ib txoj kev zoo ib puag ncig, ua tau zoo, thiab kev lag luam tsim khoom siv los txhim kho cov khoom lag luam zoo thiab lawv tus kheej kev sib tw.
Cov tshuaj tiv thaiv tsis zoo
Hygromycin byog aminoglycoside antibioti tau los ntawm Streptomycopicus. Vim tias nws cov dav - spectrum cov tshuaj tua kab mob thiab cov kab mob morotox, nws yog cov tsiaj txhu kev tshawb fawb txog kev ua haujlwm, thiab cov tsiaj cov tsiaj. Hauv kev ua yeeb yam engetic engineering, hygromycin B feem ntau yog siv los ua kev ntsuas cov cim los xaiv cov noob kab mob uas tau ua tiav nrog cov noob (xws li HORG lossis HHPH); Hauv cov tsiaj cov txiv, nws cov khoom tshuaj tua kab mob tau siv los tiv thaiv thiab kho kab mob. Txawm li cas los xij, cov tshuaj lom los ntawm hygromycin B tsis tuaj yeem tsis quav ntsej, raws li nws cov cai tsis muaj kev phom sij rau tib neeg kev noj qab haus huv thiab kev nyab xeeb ib puag ncig.
Cov kev tsis zoo hauv kev tshawb fawb kev tshawb fawb
Cytotoxicity
Hauv kev tshawb fawb caj ces, hygromycin b yog feem ntau siv los tshuaj ntsuam cov hlwb mus rau cov noob uas tsis kam. Txawm li cas los xij, txawm tias koj lub hlwb hais txog cov noob (xws li Hyosphorhlations thiab inactivates hygromycin B Tsib kuj tseem muaj kuab lom rau cov hlwb. Kev tshawb fawb tau pom tias Hygromycin B tuaj yeem ua rau lub cell agoptosis lossis necrosis, La tawg, thiab ua kom muaj kev ua haujlwm ntawm Caspase ua haujlwm. Cov tshuaj lom neeg cov tshuaj lom no yuav cuam tshuam qhov tseeb ntawm cov txiaj ntsig kev sim, cov hlwb tsis muaj txiaj ntsig (cov hlwb tiv taus tsis tseeb
Kev pheej hmoo ntawm Genetic hloov
Qhov kev txhais lus tsis raug ntawm Hygromycin B tuaj yeem nce kev pheej hmoo ntawm cov gene kev sib hloov. Thaum lub sij hawm protein protein, kev txhais lus tsis yog los ntawm Hygromycin B yuav ua rau muaj cov lus teb tsis meej lossis ua rau DNA kev puas tsuaj thiab cov kev sib hloov ntawm DNA. Txawm hais tias tam sim no tsis muaj cov pov thawj ncaj qha los qhia txog cov khoom siv hygromycin tseem xav tau kev saib xyuas, tshwj xeeb} - dose siv.
Kev Nyab Xeeb Kev Nyab Xeeb Kev Nyab Xeeb
Hygromycin B muaj qee yam kev lom zem toxic rau tib neeg lub cev, thiab kev tswj hwm kev nyab xeeb yuav tsum ua nruj ua raws li lub sijhawm ua haujlwm. Hu rau ncaj qha rau Hygromycin B hmoov los yog kev daws teeb meem yuav ua rau tawv nqaij lossis lub qhov muag lossis nqus dej los yog areerols tuaj yeem ua rau muaj kev puas tsuaj rau kev ua pa. Tsis tas li ntawd, hygromycin b tuaj yeem nqus tau lossis nqus tau cov tawv nqaij, thiab cov kws tshawb fawb thiab cov kws tshawb nrhiav kev ua haujlwm, thiab ua haujlwm ntev.
Cim npe nrov: Hygromycin B CAST 31282-04-9, Cov chaw tsim khoom, chaw tsim tshuaj paus, chaw tsim khoom, lag luam wholesale, yuav, nqe, loj, loj, rau muag