Laxogenin Hmoov, kev paub zoo li 5 - Hydroxy Laxogenin, muaj molecular qhov hnyav ntawm430.62.}}} nws tsis yog qhov tshuaj hormone .
Laxogenin feem ntau muab rho tawm los ntawm cov nroj tsuag hauv qab no:
Smilax scobinicaulis:Ib txwm muaj tshuaj noj tshuaj noj tshuaj, nws muaj los tiv thaiv cov haujlwm thiab antioxidant kev ua si .
Lwm cov nroj tsuag smilax:xws li Smilax Glora, thiab lwm yam {{} Cov nroj tsuag no tau faib dav hauv Asia thiab South America.
Cov txheej txheem rho tawm feem ntau suav nrog cov kauj ruam xws li cov khoom siv dawb, thiab thaum kawg cov khoom dawb uas muaj ntau dua lossis sib npaug ntawm 98% yog tau txais.
Raws li tus neeg tsis zoo ntawm cov tshuaj hormonal, nws tau ua kom tsis txhob muaj cov tshuaj tiv thaiv cov tshuaj uas tau dhau los, thiab yog qhov ua tau zoo rau cov neeg muab khoom siv uas tau dhau los thiab nruj me ntsis ua raws li cov tshuaj no Cov Lus Qhia . Nyob rau hauv lub neej yav tom ntej, nrog kev ua si ntawm cov kev ua kis las thiab kev kho mob rov zoo {}}
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Laxogenin Hmoov Coa
Laxogenin Hmoovyog sapoidal saponin extracted los ntawm cov nroj tsuag {{0} {{{1} nws cov haujlwm anabolic thiab lub zog tseem ceeb tuaj yeem yog cov ntsiab lus hauv qab no:
Txhawb nqa cov protein synthesis thiab nrawm rau cov leeg loj hlob
Laxogenin tseem ceeb tsub nqi ntawm cov tshuaj protein los ntawm kev ua kom nrawm dua ntawm cov tsiaj txhu ({{}} Piv txwv, thaum8- lub lim tiam Lub sijhawm ntxiv, qhov nruab nrab thickness ntawm Quadriceps Femoris tau nce los ntawm cov leeg cov pob zeb), thiab nce cov kab mob acid cov amino acids (BCAA), thiab muab cov khoom siv raw rau cov khoom siv hluavtaws {}}
Tswj cov qib cortisol thiab txo cov leeg nqaij tawg
Cortisol yog kev ntxhov siab hormone .-} kev sib txuam ntawm cov nqaij protein thiab cov khoom siv tsis zoo ntawm cortisol los ntawm ob lub tshuab tsis zoo:
Inhibition ntawm 11 - hsd1 enzyme:Txo qhov kev tsim kho ntawm cortisol hauv cov leeg thiab cov ntaub so ntswg zoo thiab txo qis qis hauv zos cortisol concentration .
Antagonizing lub glucocorticoid (gr):Thaiv cov cortisol-induced leeg cov nqaij protein degradation POSTENDING PACKAY.
Cov ntaub ntawv kuaj mob qhia tias cov tshuaj ntxiv yog txo cov tsiaj txhu (LDH)
Txhim kho kev ua tau zoo thiab kev muaj peev xwm rov qab
Laxogenin muaj peev xwm txhim kho zog sai sai thiab kev ua kom muaj kev zam txim:
Cov kev cuam tshuam luv luv (3-5 hnub):Kev tso tawm zog nce los ntawm 10-15% (hauv kev sib npaug sib npaug tuav tes ntsuas), thiab cov repetitioned lub zog nce los ntawm 20% .
Kev cuam tshuam ntev (3-4 lub lis piam):Cov nqaij ntshiv lub cev loj nce los ntawm {1.5-2.5 kg, thiab cov leeg ua kom zoo dua {}}
Nws cov khoom siv antioxidant (xws li txhim kho cov dej num ntawm sod thiab GPX) tuaj yeem ua rau muaj kev ntxhov siab thiab nrawm nrawm dua li 24 teev tom qab tawm dag zog {}}
Kev Nyab Xeeb Kev Nyab Xeeb: Tsis muaj cov tshuaj tiv thaiv kev cuam tshuam, haum rau kev siv ntev
Tsis zoo li hluavtaws sterthoids, laxogenin:
Tsis muaj kev cuam tshuam nrog cov tshuaj hormone sib npaug:There were no significant changes in hormone levels such as testosterone, estrogen, and cortisol (p>0.05).
Tsis muaj kev pheej hmoo tshuaj tshuaj:Tsis yog txiav steroid lossis cov kev ntsuas stimulant txwv los ntawm Wada .
Tsawg Hepatorenal Toxicity:Alanine AminotRansferase (alt), aspartate aminottransferase (ast), thiab creatinine creatance tus nqi nyob ruaj khov .
Cov yam ntxwv no ua rau nws xaiv cov neeg nyiam rau cov tshuaj hormones, xws li cov poj niam thiab cov hluas}
Lub hauv paus kev txawj ntse ntawm kev ua haujlwm kev paub tseeb
Txoj haujlwm ntawm Laxogenin yog raws nws cov qauv tshuaj tshwj xeeb (Selosteranols) thiab cog-muab cov yam ntxwv:
Mtor Av Kev Ua Haujlwm
Los ntawm kev tswj hwm lub phosphorholation ntawm P70S6K thiab 4E-BP1, nws txhawb nqa Ribosomal Biosynthesis.
CORTISOL Cov Kev Cai
Inhibit qhov kev ua si ntawm 11 - hsd1 enzyme thiab txo cov cortisol regeneration; Ib nrab thaiv cov kev sib txuas lus ntawm cov gr receptor .
Anti-inflammatory thiab antioxidant cuam tshuam
Inhibit lub nf-κb thiab txo cov profammatory xws li ur -6 thiab tnf-; Txhim kho lub peev xwm los tshem tawm Ros .
Daim Ntawv Thov Qhia Txog Cov Xwm Txheej thiab Kev Pom Zoo Txog Kev Pom Zoo
Qoj thiab cov leeg cov leeg
Cov pib tshiab:{{050-100 mg ib hnub, noj hauv ob zaug muab cov koob tshuaj sib faib (thaum sawv thiab tom qab kev cob qhia) {1}.
Cov neeg siv khoom siab heev:100-200 mg ib hnub, ua ke nrog ib qho {1} {{{{{.
Secmical Rehabilitation
Cov leeg nqaij:200mg ib hnub rau 12 lub lis piam, txhim kho cov leeg muaj zog (MMT) ntawm qib qib1-2.
Rov Qab Rov Qab:100mg ib hnub, ua ke nrog kho mob lub cev, ua kom luv dua lub tsev kho mob los ntawm 3 txog 5 hnub .
Muaj pab pawg tshwj xeeb
Poj Niam:50-75 mg ib hnub, tsis muaj cov kev mob tshwm sim (xws li hirsutism, ntxau).
Rau cov laus:{{025-50 mg ib ib hnub rau txhim kho tuav lub zog thiab seem.
Laxogenin HmoovPuas yog Sapoidal Sapogenin rho tawm los ntawm cov nroj tsuag . Cov qauv tshuaj xyuas thiab kev soj ntsuam cov qauv kev ua haujlwm, kev ntsuas kev ua hauv qab no thiab kev ntsuas kev ua haujlwm:
Kev Ntsuam Xyuas Zoo: Paub meej tias qhov muaj ntawm Laxogenin
Nyias-Txheej chromatography (TLC)
Lub hauv paus ntsiab lus:Kev sib cais yog nqa los ntawm kev coj kom zoo dua ntawm cov kev faib tawm coefficients ntawm cov theem sib txawv hauv cov theem sib txawv (silica gel phaj) .
Kev ua haujlwm:
Cov qauv yog yaj nyob rau hauv Methanol lossis chloroform thiab pom ntawm silica gel phaj .
Tsim Tus Neeg Sawv Cev: Chloroform - Dej Methanol - Dej (6: 3: 1, V / V) los yog cov tshuab zoo sib xws}
Kev Txhim Kho Cov Xim: Tshuaj Tsuag 10% Sulfuric acid etanol daws, kub nws thiab saib cov nqi rf yuav tsum tau ua raws li cov khoom txheem) .
Qhov Zoo:Sai thiab tus nqi qis, haum rau kev kuaj pib.
Kev ua kom zoo ua kua chromatography (HPLC)
Lub hauv paus ntsiab lus:Cov lus sib txuas tau sib cais los ntawm cov txheej txheem siab ua kua thiab tsim nyog txiav txim siab los ntawm UV ntes lossis cov ntsuas huab hwm coj ntau ntxiv (MS).
Kev ua haujlwm:
Chromatographic kem: C18 thim rov qab-theem (4 . × 250mm, 5μm).
Mobile theem: Acetoniturile - Dej (50:50, V / V), muaj 0 {{4%} 1%} 1%} 1%} 1% formic acid.
Kev Tshawb Nrhiav Wavelength: 210NM (yam ntxwv nqus tau ncov ntawm steroid compound) .
Kev muag khoom: Piv nrog cov khoom lag luam qauv kom paub meej cov laxogenin ncov .
Qhov Zoo:Siab rhiab, kev daws teeb meem siab, thiab muaj peev xwm los kuaj cov impurities ib txhij .
Kev Ntsuam Xyuas Quantitative: Kev txiav txim ntawm Laxogenin cov ntsiab lus
HPLC-UV Sab Nraud Txuj Ci
Kauj ruam:
Npaj ib txoj kev ntawm cov concentration cov kev daws teeb meem (xws li 10, 20, 50, 100, 200, 200ug / ml) {5 {5 5}
Kos cov txheej txheem nkhaus txheem (thaj chaw siab tshaj vs siab) .
Tom qab cov qauv yog ultrasonically muab rho tawm nrog methanol, nws tau txhaj rau kev tsom xam thiab cov ntsiab lus tau suav .
Kev txheeb xyuas cov qauv:
Linear ntau: {{{{10-200}} ug / ml (r² ntau dua lossis sib npaug ntawm 0. 999).
Rov qab tus nqi: 95% {-105}% (spiked rov qab ntsuas) .
Precision: RSD tsawg dua lossis sib npaug rau 2% (nyob rau hauv ib hnub thiab inter-hnub rov ua dua tshiab) {}
Ultra-kev ua kua ua kua chromatography-tandem loj spectrometry (Uplc-MS / MS)
Muaj feem cuam tshuam cov xwm txheej:Qaum Kev Ntsuam Xyuas lossis cov nyom matrices (xws li cov nroj tsuag rho tawm) .
Mob:
Chromatographic Hauv PACK: Yuav Uplc 1 C18 (2 . × 50 hli, 1.7μm).
Mobile theem: acetonitrile -0.1}}% formic acid dej (gradient elution).
Ion Source: Electrospray ionization (esi), muaj kev zoo ion hom .
Saib xyuas ion khub: m / z 431 . 6 → 139.1 (kom muaj tseeb.
Qhov Zoo:Rhiab li ntawm mus rau NG / ML qib, muaj zog tiv thaiv kev cuam tshuam muaj peev xwm .
Cov Txheej Txheem Txheej Txheem: Paub meej cov qauv molecular
Nuclear Sib Nqus Revonance spectroscopy (NMR)
Cov qauv npaj: DaRalated chloroform (cdcl₃) thiab ntxiv tetramethilsilane (tms) raws li tus qauv sab hauv .
Cov ntsiab lus:
¹H NMR: Paub meej tias cov tshuaj ua haujlwm ntawm hydrogen atoms (e {{}} huag ntawm}}} ppm) .
¹³c NMR: Paub meej cov pa roj carbon (xws li tus yam ntxwv siab ntawm tus kheej ntawm tus kheej tus kheej) .
2D NMR (HSQC, HMBC): Kev Tshawb Fawb Hydrogen-Carbon Kev Sib Txuas Kev Sib Koom Zoo {}}
Cov ntawv sib piv database: piv nrog cov ntaub ntawv NMR ntawm Laxogenin (xws li pubchem ID: 10950057).
Pawg Spectrometry (MS)
High-decorution cass spectrometry (HRMS)
Txiav txim siab cov molecular hnyav (theoretical tus nqi: 430. 625, ntsuas tus nqi yuam kev tsawg dua los yog sib npaug rau 5ppm).
Paub meej tias cov mis molecular (c₂₇h₄₂o₄) .
Tandem Pawg Spectrometry (MS / MS)
Fragment ion kev tsom xam (xws li M / Z 431 → 331
Kev ntsuas ntsuas roj ntsha: Txheeb xyuas qhov ua tau zoo
Protein Synthesis sim
Qauv: MyBlasts ntawm C2C12 nas .
Txoj kev:
Cov kab mob tau raug coj mus kuaj hauv DMEM muaj 10% fbs thiab sib txawv rau hauv iSotubes .
Ntxiv laxogenin (1-100 μm) thiab kho rau 24 teev .
Kuaj tus nqi ntawm cov khoom siv protein (xws li ³ h-leucine incorporation txoj kev) .
Kev Tswj Xyuas Zoo: Insulin (100nm) .
Anti-incamicatory sim
Qauv: Raw264 . 7 mactropcages.
Txoj kev:
Lps (1l / ml) induces ib qho kev hloov pauv tsis muaj mob .
Tom qab pretreatic nrog laxogenin (10. uv {-6 thiab tnisa txoj kev).
Tsiaj sim
Tsim: SD nas, ib qho {8-}}}} lub zog zog kawm qauv {}}
Pab pawg: pawg tswj hwm, Laxogenin Group (100mg / kg / hnub, gavage) {{1}.
Qhov taw qhia:
Leeg phaus (quadriceps, gastrocnius) .
Tuav lub zog tuav (hluav taws xob khawm kev cob qhia) .
Ntshav dej CORTISOL (ELISA Txujcub)
Cov ntsiab lus tseem ceeb ntawm kev tswjhwm zoo
Purity txheem
HPLC cheeb tsam tus qauv no ntau dua lossis sib npaug ntawm 98%.
Hnyav Hlau Nrhiav Kom Tau
Kev txiav txim siab ntawm kev coj ua (PB) tsawg dua lossis sib npaug ntawm 0 {{{}}}}}}}}}} 002% thiab arsenic (li) tsawg dua lossis sib npaug ntawm ICP-MS Txuj.
Microbial txwv
Tag nrho colony suav tsawg dua los yog sib luag rau 1000cfu / g, pwm thiab poov xab tsawg dua los yog sib npaug rau 100cfu / g.
Cov kuab khoom seem
GC txoj kev rau kev kuaj cov khoom seem ntawm methanol, chloroform, thiab lwm yam . (hauv kev ua raws li cov lus qhia .
Cov lus qhia rau kev xaiv qauv
Kev Ntsuas Xyuas sai:TLC + HPLC-UV .
Ua kom meej tseeb:Uplc-MS / MS (Haum rau cov ntsiab lus tsawg cov ntsiab lus) .
Kev Pom Zoo:NMR + HRMs (Cov Khoom Siv Kev Tsim Kho Kev Txhawb Nqa) .
Cov Lus Qhia Ua Haujlwm:Kev sim ntawm tes + Tsiaj sim (sib xyaw ua ke nrog kev tshawb fawb) .
Kev Tshawb Xyuas Kev Ntsuam Xyuas: Daim ntawv thov ntawm Laxogenin hauv kev ua si ncaws pob
Ib pab neeg tshawb fawb pom los ntawm kev tsom xam kom muaj kev txheeb xyuas HPLC-MS / MS uas tom qab ncaws pob ntxiv Laxogenin (100mg / hnub) rau 8 lub lis piam:
Ntse lub cev loj nce los ntawm 2 . 1kg (dexa scan).
Lub thickness ntawm lub quadriceps femoris nce los ntawm 10% (raws li tau ntsuas los ntawm MRI).
CK qib txo los ntawm 35% tom qab qoj ib ce (p<0.01).
There was no significant change in serum cortisol concentration (p>0.05).
Txoj kev tshawb no tau txheeb xyuas qhov ua tau zoo ntawmLaxogenin Hmoovhauv kev txhawb cov leeg kev loj hlob thiab nrawm nrawm dua, tsis muaj kev pheej hmoo ntawm kev cuam tshuam hormonal hormonal.
Cim npe nrov: Laxogenin Hmoov, Cov Khoom Siv, Chaw Tsim Khoom, Hoobkas, lag luam Wholesale, Yuav, Nqe, loj, loj, rau muag