Shaanxi BLOOM Tech Co., Ltd. yog ib tug ntawm cov uas paub txog manufacturers thiab lwm tus neeg ntawm atrial natriuretic peptide nas cas 88898-17-3 nyob Suav teb. Txais tos rau cov lag luam wholesale bulk zoo atrial natriuretic peptide nas cas 88898-17-3 muag ntawm no los ntawm peb lub hoobkas. Kev pabcuam zoo thiab tus nqi tsim nyog muaj.
Atrial natriuretic peptide nas, tseem hu ua cardiac natriuretic hormone thiab atrial natriuretic peptide, belongs rau tsev neeg ntawm natriuretic peptides. Qhov no yog ib yam ntawm active polypeptide synthesized, khaws cia thiab secreted los ntawm lub atria, uas yog tseem hu ua atrial natriuretic factor (ANF) los yog atrial natriuretic peptide (ANP). Nws muaj zog natriuretic, diuretic, vasodilatory, ntshav siab - txo qis thiab antirenalin -angiotensin system thiab antidiuretic cov tshuaj hormones. Kev ntsuas ntshav ANP kev ua haujlwm yog siv los kuaj xyuas thiab saib xyuas cov chav kawm thiab cov txiaj ntsig ntawm cov neeg mob plawv tsis ua haujlwm.
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| Qhov ntom | 1.54 ± 0.1 g / cm3 (Kev kwv yees) |
| Kev cia khoom | -20 degree |
| Dej solubility | Soluble rau 1 mg / ml hauv dej |
| Purity (HPLC) | Ntau dua lossis sib npaug li 98.0% |
| Cov ntsiab lus acetate | Tsawg dua lossis sib npaug li 12.0% |
| Cov ntsiab lus dej | Tsawg dua los yog sib npaug rau 8.0 |
| Cov ntsiab lus Peptide | Ntau dua lossis sib npaug rau 80.0 |
| Endotoxin | Tsawg dua lossis sib npaug li 50EU / mg |
| Kev txheeb xyuas cov amino acid muaj pes tsawg leeg | Tsawg dua lossis sib npaug ± 10% |
| tshwm sim | hmoov dawb |
Atrial natriuretic peptide nasFeem ntau yog secreted los ntawm atrial myocytes, hlwb natriuretic peptide feem ntau yog secreted los ntawm ventricular myocytes, thiab C-type natriuretic peptide yog tsuas yog secreted los ntawm vascular endothelial hlwb thiab exerts vasodilatory thiab antiproliferative teebmeem hauv zos. Plasma cardiac natriuretic peptide muaj ntau yam cuam tshuam ntawm vasodilation, diuresis, thiab antagonism ntawm cov tshuaj hormones neuroendocrine.
Txij li thaum atrial dilatation yog lub ntsiab ua rau AVP tso tawm, qhov concentration ntawm ANP yog ze ze rau sab laug atrial siab thiab pulmonary hlab ntsha systolic siab.
Ua kom siab: pom nyob rau hauv qhov tseem ceeb kub siab, kab mob coronary artery, plawv tsis ua hauj lwm, supraventricular tachycardia, cirrhosis ntxov ntxov, bronchial hawb pob, chronic obstructive pulmonary kab mob, aldosteronism, thiab raum kab mob. Hauv kev mob hnyav heev, nce ANP muaj qhov tshwj xeeb kwv yees tus nqi rau LV tsis ua haujlwm thiab kev tuag hauv AMI, thiab nce siab ntawm ANP hauv cov neeg mob uas mob myocardial infarction nyob rau theem subacute qhia txog qhov tsis zoo ntev - lub sijhawm kwv yees.
Txo: pom nyob rau hauv hyperthyroidism, atrial fibrillation.
Cov kev ceev faj
Kev sau cov qauv yuav tsum tau ua raws li cov xwm txheej zoo ib yam li ntau li ntau tau, piv txwv li, tib lub sijhawm txhua hnub, tom qab pw tsaug zog thiab tseem 15min, txhawm rau kom muaj txiaj ntsig zoo sib xws.Kev ua haujlwm ntau dhau thiab tachycardia yuav ua rau ANP.Kev mob siab rau lub raum yuav tsum tsis suav nrog, qhov twg ANP tuaj yeem khaws cia vim leej twg tuav.
Atrial natriuretic peptide nasyog peptide hormone synthesized los ntawm lub plawv thaum lub ventricular phab ntsa yog dilated los yog strained. Nws yog ib qho tseem ceeb ntawm cov tshuaj hormones hauv kev tswj cov kua dej hauv lub cev, lub cev sodium sib npaug, ntshav siab, thiab tuaj yeem ua lub luag haujlwm tiv thaiv lub plawv. Thaum lub ventricular phab ntsa yog dilated los yog stretched, cardiac natriuretic peptide yog tso rau hauv cov hlab ntsha, uas nyob rau hauv lem muaj cov nyhuv ntawm kev tswj lub plawv ua hauj lwm thiab dilating cov hlab ntsha. Yog tias qib ntawm cardiac natriuretic peptide nce, nws yuav ua rau muaj kev nce ntxiv hauv lub plawv, uas tuaj yeem ua rau lub plawv tsis ua haujlwm.
Lub ntsiab kev ua tau zoo ntawm Atrial Natural Peptide nas
balanced dilatation ntawm cov hlab ntsha thiab cov hlab ntsha, txo cov anterior thiab posterior load ntawm lub plawv, sai sai ntawm dyspnea.
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Nruab nrab diuresis thiab sodium excretion, txo circulating ntim load, nce raum pom degree.
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Comprehensively antagonize lub cardiotoxicity tsim los ntawm kev ua kom lub neuroendocrine system.
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Ncua kev kho plawv, tiv thaiv myocardial hyperplasia, hypertrophy thiab interstitial fibrosis, thiab txo cov sijhawm ntev - mob..
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Tsis muaj qhov zoo inotropic thiab lub plawv dhia zoo, tsis muaj kev nce hauv myocardial oxygen noj.
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Atrial natriuretic peptide nasyog ib qho tseem ceeb peptide hormone uas ua lub luag haujlwm tseem ceeb hauv kev tswj cov kua dej sib npaug thiab ntshav siab. Cov hauv qab no yog ib txoj kev sib xyaw ua ke.
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Synthesis cov kauj ruam
Feem ntau, atrial natriuretic peptide tuaj yeem ua ke los ntawm cov khoom siv -theem synthesis (khoom-theem synthesis) lossis kua-theem synthesis (kev daws-theem synthesis). Hauv cov khoom-theem synthesis, cov saw peptide yog tsim los ntawm maj mam ntxiv cov amino acids rau cov cab kuj.
Qhov sib lawv liag ntawm ANP yog luv luv thiab paub, feem ntau muaj 28 amino acid residues.
Thaum lub sij hawm synthesis, txhua cov amino acid yuav tsum muaj kev tiv thaiv cov pab pawg kom tsis txhob muaj kev phiv. Cov pab pawg tiv thaiv no yuav tsum tau muab tshem tawm tom qab kev sib txuas ua tiav kom tso cai rau cov amino acid txuas mus rau peptide saw.
Cov saw peptide yog cov txheej txheem sib xyaw ua ke -los ntawm - kauj ruam hauv kev txiav txim ntawm ANP ib ntus raws li cov txheej txheem kev sib txuas thiab cov khoom siv xaiv. Txhua amino acid ntxiv yuav tsum tau rov ua cov kauj ruam ntawm kev tiv thaiv, kev sib txuas, thiab kev tiv thaiv.
Tom qab kev sib txuas ua tiav, peptide yuav tsum tau ua kom huv si thiab ua kom lub cev kom huv si thiab raug cov qauv ntawm cov khoom.
Molecular Structure: Kev sib xyaw thiab kev hloov pauv ntawm Peptide Chains
Asim natriuretic peptidenasyog polypeptide hormone synthesized los ntawm nas atrial hlwb thiab belongs rau tsev neeg natriuretic peptide. Nws zoo sib xws hauv cov qauv rau tib neeg atrial natriuretic peptide (hANP) tab sis muaj ntau hom sib txawv. Kev kawm txog nws cov khoom siv tshuaj lom neeg yog qhov tseem ceeb rau kev nkag siab txog nws txoj haujlwm physiological thiab muaj peev xwm siv tau. Cov kev soj ntsuam hauv qab no yog ua los ntawm plaub qhov loj: cov qauv molecular, physicochemical zog, receptor binding, thiab kev ua haujlwm lom neeg.
Lub cev thiab tshuaj lom neeg: Solubility thiab ruaj khov-rANP yog soluble hauv dej thiab physiological saline, nrog rau solubility ntau dua 10 mg/mL. Nws solubility cuam tshuam loj heev los ntawm pH: nyob rau hauv acidic tej yam kev mob (pH <5), rANP yuav muaj ib tug txo nyob rau hauv solubility vim protonation; nyob rau hauv alkaline tej yam kev mob (pH> 9), disulfide bonds yuav tawg, ua rau lub depolymerization ntawm polypeptide saw. Yog li ntawd, kev cia thiab siv rANP yuav tsum nruj tswj pH ntau yam (feem ntau pH 6.0 - 8.0).rANP yog kub-sensitive. Thaum khaws cia ntawm -20℃hauv tub yees, nws cov haujlwm tuaj yeem khaws cia rau ntau xyoo.
Txawm li cas los xij, ntawm 4 degree, kev ua haujlwm yuav txo qis los ntawm 5% mus rau 10% txhua lub lim tiam; Nyob rau hauv chav tsev kub (25 degree), qhov kev poob haujlwm yuav siab tshaj 30% hauv 24 teev.
Ntxiv mus, rov ua kom khov thiab thawing tuaj yeem ua rau rANP sib sau ua ke thiab tsim cov dej tsis sib haum. Yog li ntawd, nws raug pom zoo kom aliquot thiab siv nws ib zaug xwb.
rANP yog qhov rhiab heev rau proteases (xws li trypsin thiab chymotrypsin). Hauv cov tshuaj uas muaj 0.1% trypsin, rANP tuaj yeem degraded tag nrho hauv 30 feeb ntawm 37 degree. Yog li ntawd, hauv kev sim lossis kev siv tshuaj kho mob, nws yog ib qho tsim nyog kom tsis txhob muaj kev sib raug zoo nrog proteases lossis ntxiv protease inhibitors (xws li PMSF) rau kev tiv thaiv.
Cov ntawv thov thiab cov kev sib tw
Cov khoom siv tshuaj lom neeg ntawm rANP ua rau nws muaj peev xwm kho mob ntshav siab, plawv tsis ua haujlwm, thiab kab mob raum. Txawm li cas los xij, nws cov hom tshwj xeeb (kev ua haujlwm qis dua ntawm tib neeg receptors), luv ib nrab -lub neej (txog 5 - 10 feeb), thiab proteinase rhiab heev txwv nws daim ntawv thov kev kho mob. Tam sim no, cov kws tshawb fawb tau ua kom zoo dua rANP los ntawm cov tswv yim hauv qab no: Tsim NPR-Ib qho kev xaiv sib txawv (xws li rANP(REA18)), txo qhov kev sib haum xeeb los ntawm NPR-C; Kev hloov kho tshuaj (xws li pegylation) kom txuas ntxiv ib nrab - lub neej; Nanocarrier xa mus rau txhim khu kev ruaj ntseg.
Receptor Binding: Lub luag haujlwm Dual ntawm NPR-A thiab NPR-C
rANP exerts nws cov teebmeem los ntawm ob lub membrane receptors:
NPR-A (Natriuretic Peptide Receptor-A)
Kev sib txuas ntawm rANP rau NPR-A yog siab (Kd ≈ 1-10 nM), ua kom muaj kev ua haujlwm ntawm guanylate cyclase nyob rau hauv intracellular ntu ntawm receptor thiab catalyzing tsim cGMP los ntawm GTP. cGMP ua tus xa xov thib ob, tswj cov ion channel (xws li Na⁺ channels hauv lub raum tubular epithelial hlwb) thiab cov haujlwm enzyme (xws li phosphodiesterase) los ntawm kev ua kom muaj protein ntau kinase G (PKG), thaum kawg txhawb nqa sodium excretion, diuresis, thiab vasodilation.
Cov kev tshawb fawb tau pom tias qhov ua kom zoo ntawm rANP ntawm nas NPR-A yog 1.2-1.5 npaug ntawm hANP.
NPR-C (Natriuretic Peptide Receptor-C)
NPR-C tsis muaj kev ua haujlwm enzymatic thiab feem ntau ua haujlwm ua tus txais kev tshem tawm, degrading rANP los ntawm endocytosis. Kev khi ntawm rANP rau NPR-C tuaj yeem ua rau lub cev hloov pauv hauv lub cev (xws li inhibition ntawm adenylate cyclase), tab sis cov nyhuv no tsis muaj zog dua li ntawm NPR-A.
Los ntawm kev tsim cov noob caj noob ces ntawm rANP (xws li rANP(G16R, A17E, Q18A)), lub peev xwm khi rau NPR-C raug txo los ntawm kwv yees li 200 npaug, thaum tuav lub peev xwm ua kom muaj peev xwm ntawm NPR-A.
Kev Ua Txhaum Cai: Vasodilation thiab Sodium Excretion thiab Diuresis
Kev ua haujlwm lom neeg ntawm rANP feem ntau ua tiav los ntawm cGMP txoj hauv kev:
Vasodilation
rANP tuaj yeem nthuav dav aorta, lub raum hlab ntsha, thiab cov hlab ntsha mesenteric ntawm cov nas, nrog rau EC50 ntawm kwv yees li 0.1 - 1 nM. Nws mechanisms muaj xws li:
Kev so ncaj qha ntawm vascular du leeg hlwb;
Inhibition ntawm vascular contraction induced los ntawm endothelin-1 (ET-1);
Kev txo qis ntawm vascular du nqaij cell proliferation (los ntawm GATA4-dependent txoj kev los inhibit endothelin noob qhia).
Na- txo thiab diuresis
Intravenous txhaj ntawm rANP (0.325 ug / min) tuaj yeem ua rau cov nas tso zis sodium los ntawm 3 - 5 zaug thiab zis ntim los ntawm 2 - 3 zaug. Nws cov chaw ua haujlwm suav nrog:
Glomerulus: Los ntawm dilating lub afferent arteriole, nws ua rau kom cov ntshav khiav;
Nephron: inhibiting Na⁺-K⁺-2Cl⁻ co-transporter (NKCC2) thiab Na⁺/H⁺ exchanger (NHE3), txo Na⁺ reabsorption;
Sau cov duct: inhibiting kev qhia ntawm aquaporin 2 (AQP2), txo cov dej reabsorption.
Kev tswj ntshav siab
rANP tuaj yeem txo qhov siab ntawm cov hlab ntsha ntawm cov nas los ntawm 10 - 20 mmHg, nrog rau qhov cuam tshuam ntev li 30 - 60 feeb. Nws cov nyhuv antihypertensive yog synergistically ntsig txog vasodilation thiab sodium txo, thiab tsis yog nyob ntawm kev hloov hauv lub plawv dhia.
Cov khoom siv tshuaj lom neeg ntawm cov nas atrial natriuretic peptide (rANP) qhia txog cov yam ntxwv ntawm cov tshuaj hormones polypeptide: cov qauv- nyob ntawm kev ua haujlwm, ib puag ncig rhiab heev, thiab receptor- kho cov teeb liab hloov pauv. Kev nkag siab tob txog nws cov khoom siv physicochemical thiab cov teebmeem lom neeg tsis yog tsuas yog muab cov cuab yeej rau kev tshawb fawb yooj yim xwb tab sis kuj tseem qhia txog txoj hauv kev rau kev txhim kho cov tshuaj mob plawv tshiab.
FAQ
Atrial-Natriuretic Peptide(rat) yog 28-amino-acid peptide hormone endogenously tsim thiab secreted los ntawm atrial myocytes ntawm nas, belongs rau tsev neeg natriuretic peptide. Nws muaj tus yam ntxwv cyclic qauv tsim los ntawm disulfide daim ntawv cog lus ntawm 7th thiab 23rd amino acid residues, uas yog qhov tseem ceeb rau kev tswj hwm nws cov khoom lom. Nrog rau qhov hnyav molecular ntawm kwv yees li 3080 Da thiab cov qauv tsim tshwj xeeb, nws yog ib qho cuab yeej tseem ceeb rau kev tshawb fawb txog kev mob plawv thiab lub raum physiological hauv cov qauv nas.
Atrial-Natriuretic Peptide(rat) ua lub luag haujlwm tseem ceeb hauv kev tswj cov hlab plawv thiab lub raum ntawm cov nas los ntawm kev khi rau cov natriuretic peptide receptors tshwj xeeb (NPR-A, NPR-B, NPR-C). Nws lub luag haujlwm tseem ceeb suav nrog kev txhawb nqa natriuresis (sodium excretion) thiab diuresis, dilating peripheral cov hlab ntsha kom txo cov ntshav siab, tswj cov ntshav ntim thiab lub plawv dhia, thiab inhibiting renin -angiotensin-aldosterone system (RAAS) kom tswj lub cev kua thiab electrolyte tshuav. Cov haujlwm no ua rau nws siv dav hauv kev kawm kub siab, lub plawv tsis ua haujlwm, thiab lub raum tsis ua haujlwm hauv kev sim tshuaj nas preclinical.
Atrial-Natriuretic Peptide(rat) feem ntau yog siv nyob rau hauv ob qho tib si nyob rau hauv vivo thiab hauv vitro preclinical kev tshawb fawb nrog nas. Rau hauv vivo thwmsim, nws feem ntau yog siv los ntawm kev txhaj tshuaj intravenous, txhaj tshuaj subcutaneous, lossis txhaj tshuaj intraperitoneal los soj ntsuam nws cov teebmeem ntawm cov nas hemodynamics, lub raum ua haujlwm, thiab cov tshuaj hormone secretion. Rau kev tshawb fawb hauv vitro, nws tau ntxiv rau cov kab lis kev cai ntawm tes (piv txwv li, nas cardiomyocytes, lub raum tubular epithelial hlwb) txhawm rau tshawb nrhiav nws cov txheej txheem molecular, xws li kev ua kom receptor, teeb liab txoj hauv kev, thiab kev tswj xyuas cov kev ua haujlwm ntawm tes.
Atrial-Natriuretic Peptide (rat) feem ntau yog muab los ua cov hmoov dawb lyophilized nrog purity ntau dua lossis sib npaug li 98% (txiav txim siab los ntawm HPLC), uas yooj yim soluble hauv dej thiab ib txwm ntsev tab sis tsis ruaj khov hauv cov kuab tshuaj organic. Nws yog rhiab heev rau kub, proteases, thiab pH hloov; Yog li, nws yuav tsum tau muab khaws cia rau hauv lub thawv kaw, tsaus nti ntawm -20℃lossis qis dua kom tsis txhob degradation. Reconstituted daws yuav tsum tau siv tam sim ntawd los yog khaws cia ntawm 2-8℃tsis pub tshaj 72 teev. Tsis tas li ntawd, nws qhov ntau npaum li cas hauv kev sim yuav tsum tau hloov kho raws li cov kab mob nas, hnub nyoog, thiab cov hom phiaj kev sim kom ntseeg tau cov txiaj ntsig zoo.
Cim npe nrov: atrial natriuretic peptide nas cas 88898-17-3, lwm tus neeg, manufacturers, hoobkas, lag luam wholesale, yuav, nqe, tej, kev muag khoom