Calcitonin salmonyog hmoov dawb nyob rau hauv chav tsev kub, nrog ib tug molecular mis ntawm C145H240N42O38S, CAS 47931-85-1, thiab ib tug molecular luj ntawm kwv yees li 3431.97. Tsis tshua muaj solubility nyob rau hauv dej, thaum lub siab solubility nyob rau hauv acidic daws yog ib tug peptide hormone, thiab nws viscosity tej zaum yuav muaj feem xyuam rau intermolecular kev sib tshuam thiab molecular qauv. Surface nro yog qhov attraction ntawm nto molecules thaum ib yam khoom los rau hauv kev sib cuag nrog ib tug roj los yog kua. Rau peptide cov tshuaj hormones, lawv qhov nro saum npoo yuav cuam tshuam nrog molecular polarity thiab hnyav cuam tshuam. Nws tau siv dav hauv kev kho cov kab mob xws li osteoporosis thiab hypercalcemia, thiab nws cov txiaj ntsig yuav txawv ntawm cov tib neeg thiab cov kab mob sib txawv. Nws yog peptide hormone rho tawm los ntawm salmon, uas muaj lub luag haujlwm ntawm kev tswj cov calcium thiab phosphorus metabolism, inhibiting osteoclast kev ua, thiab yog siv los kho cov kab mob xws li pob txha thiab hypercalcemia.
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Customized Bottle Caps Thiab Corks:
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Tshuaj Formula |
C145H240N44O48S2 |
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Exact Mass |
3947 |
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Molecular Luj |
3949 |
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m/z |
3431 (100.0%), 3432 (77.9%), 3430 (63.8%), 3433 (40.1%), 3432 (16.3%), 3433 (12.3%), 3431 (10.4%), 3433 (9.9%), 3433 (9.0%), 3434 (8.8%), 3434 (7.7%), 3434 (7.0%), 3434 (6.6%), 3432 (6.3%), 3432 (5.8%), 3434 (5.0%), 3435 (3.2%), 3435 (3.0%), 3435 (3.0%), 3432 (2.8%), 3433 (2.1%), 3432 (1.8%), 3431 (1.8%), 3435 (1.7%), 3434 (1.6%), 3432 (1.6%), 3434 (1.5%), 3434 (1.5%), 3433 (1.4%), 3435 (1.3%), 3433 (1.3%), 3433 (1.2%), 3435 (1.2%), 3431 (1.2%), 3435 (1.2%), 3435 (1.1%), 3433 (1.0%), 3431 (1.0%) |
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Elemental Analysis |
C, 50.75; H, 7.05; N, 17.96; O, 22.38; S, 1.87 |
Txoj kev rau synthesizingCalcitonin salmonnyob rau hauv lub chaw soj nstuam muaj cov txheej txheem peptide synthesis, nrog rau cov kauj ruam xws li amino acid condensation, deprotection, cleavage, thiab purification. Nov yog cov kauj ruam ntxaws ntxaws rau kev ua kom muaj peev xwm ntawm salmon calcitonin hauv chav kuaj:
Amino acid condensation: Nyob rau hauv qhov kev txiav txim ntawm tus neeg sawv cev condensation (xws li DCC lossis EDC), pawg carboxyl ntawm cov amino acid tau txais cov tshuaj tiv thaiv condensation nrog cov amino pawg ntawm nws cov amino acid uas nyob ib sab, tsim ib daim ntawv cog lus peptide. Qhov no tuaj yeem sawv cev los ntawm kev sib npaug hauv qab no:
(H2N-CH(R)-COOH+H2N-CH(R ')-COOH+X → H2N-CH(R)-CONH-CH(R')-COOH+XH)
Ntawm lawv, (H2N-CH (R) - COOH) thiab (H2N-CH (R ') - COOH) yog cov amino acids koom nrog cov tshuaj tiv thaiv, (X) yog tus neeg sawv cev condensation, thiab (XH) yog cov khoom los ntawm tus neeg ua haujlwm condensation.
Deprotection: Cov tshuaj sib npaug rau deprotection yuav txawv nyob ntawm cov pab pawg tiv thaiv siv. Piv txwv li, yog p-methoxyphenylpropionic acid yog siv los ua pab pawg tiv thaiv, pab pawg tiv thaiv tuaj yeem raug tshem tawm los ntawm kev sib npaug hauv qab no:
(H2N-CH(R)-COO-(C6H4) -OCH3+H → H2N-CH(R)-COOH+(C6H4) -OH)
Txiav: txiav cov tshuaj reagents (xws li cyanide bromide los yog anhydride) hnov mob nrog cov khoom siv -theem kev txhawb nqa los txiav peptide chains tawm ntawm cov khoom-theem txhawb. Qhov no tuaj yeem sawv cev los ntawm kev sib npaug hauv qab no:
(H2N-CH(R)-CONH-CH(R ')-COOH+Y → H2N-CH(R)-CONH-CH(R')-COY+HX)
Ntawm lawv, (Y) yog qhov txiav reagent, thiab (HX) yog cov khoom siv los ntawm kev txiav reagent.
Purification: Cov txheej txheem purification muaj ntau yam kev sib cais technologies, xws li gel filtration, ion exchange and reverse phase chromatography. Cov thev naus laus zis no feem ntau yog nyob ntawm qhov sib txawv ntawm qhov loj me me, nqi, thiab hydrophobicity kom cais cov khoom sib txawv.
Cov kev sib npaug tshuaj tshwj xeeb nyob ntawm cov txheej txheem sib cais thiab cov xwm txheej siv.
1. Cov khoom siv thiab cov tshuaj reagents
Calcitonin salmonextract los yog lwm yam ntaub ntawv ntsig txog
Amino acids, condensation agents, deprotection agents, txiav reagents, thiab purification reagents xav tau rau cov tshuaj synthesis
2. Kev sim cov kauj ruam
(1) Kev kho mob ntawm salmon extract: Kev kho mob ua ntej ntawm salmon extract, xws li ntxuav, crushing, centrifugation, thiab lwm yam, yog nqa tawm kom tau cov ntaub so ntswg xav tau.
(2) Kev sib cais thiab kev ua kom huv: Siv cov txheej txheem kev sib cais thiab kev ua kom huv xws li centrifugation, filtration, extraction, thiab lwm yam kom cais cov ntses salmon calcitonin los ntawm cov ntaub so ntswg.
(3) Amino acid condensation: Txuas cov amino acids nyob rau hauv salmon calcitonin nyob rau hauv ib qho kev txiav txim los tsim polypeptide chains. Cov kauj ruam no feem ntau yuav tsum tau siv cov tshuaj condensation, xws li DCC (Dicyclohexylcarbodiimide) lossis EDC (1-ethyl-3- (3-dimethylaminopropyl) carbodiimide).
(4) Kev tiv thaiv kev tiv thaiv: Thaum lub sij hawm condensation txheej txheem, sab chains thiab carboxyl pawg ntawm cov amino acids feem ntau xav tau kev tiv thaiv kom tsis txhob tshwm sim ntawm sab tshwm sim. Yog li ntawd, tom qab condensation tiav lawm, nws yog ib qhov tsim nyog los tshem tawm cov pab pawg neeg tiv thaiv thiab kho cov qauv qub ntawm cov amino acids. Txoj kev deprotection feem ntau yog nyob ntawm kev tiv thaiv puag siv.
(5) Txiav: Cov saw peptide synthesized raug txiav tawm ntawm cov khoom-theem txhawb kom tau txais peptides dawb. Cov kauj ruam no feem ntau siv txiav cov tshuaj xws li cyanide bromide lossis anhydride.
(6) Purification: Cov khoom siv salmon calcitonin yog purified los ntawm cov cuab yeej purification tsim nyog, xws li gel filtration, ion exchange los yog thim rov qab theem chromatography, kom tshem tawm impurities thiab unreacted raw khoom.
(7) Kev soj ntsuam zoo: Mass spectrometry, nuclear magnetic resonance thiab lwm yam txuj ci yog siv los soj ntsuam cov qauv thiab purity ntawm synthesized salmon calcitonin, xyuas kom meej tias nws yog raws li lub hom phiaj molecule thiab ua tiav cov purity yuav tsum tau.
Txias kom qhuav los yog tshuaj tsuag kom qhuav: khov kom qhuav los yog tsuag kom qhuav cov ntses salmon calcitonin purified kom tshem tawm cov dej thiab tau txais cov qauv qhuav.
(8) Ntim thiab khaws cia: ntim cov salmon qhuav calcitonin rau hauv lub thawv tsim nyog thiab khaws cia rau hauv qhov chaw qhuav, txias.
Keeb kwm kev loj hlob ntawmCalcitonin salmontuaj yeem taug qab mus rau xyoo 1961, thaum Canadian physiologists Coop thiab lwm tus thawj zaug pom thiab piav qhia txog calcitonin. Lawv pom los ntawm kev sim uas calcitonin yog ib yam tshuaj uas tso tawm los ntawm cov thyroid caj pas uas muaj cov txiaj ntsig ntawm kev txo cov calcium hauv cov ntshav. Qhov kev tshawb pom no tau tsim lub hauv paus rau kev tshawb fawb tom ntej thiab siv calcitonin.
Tom qab ntawd, Zondek thiab Ucko tau luam tawm cov kev tshawb fawb zoo sib xws hauv xyoo 1932, thiab lawv kuj tau tshawb pom cov tshuaj zoo ib yam li calcitonin, tab sis lawv cov ntawv tsis tau txais kev lees paub thiab kev tshaj tawm.
Txog xyoo 1961, Copp et al. ntxiv tau lees paub qhov muaj nyob ntawm calcitonin los ntawm kev sim ntawm cov ntshav calcium siab perfusion nyob rau hauv cov thyroid thiab parathyroid qog ntawm cov dev, thiab hu ua calcium (CT). Qhov kev tshawb pom no tau suav hais tias yog qhov tseem ceeb hauv kev tshawb fawb ntawm calcitonin.
Nrog rau kev sib sib zog nqus ntawm kev tshawb fawb ntawm calcitonin, tib neeg tau tshawb fawb ntau ntawm nws cov qauv molecular thiab cov txheej txheem ntawm kev ua. Ntau qhov chaw ntawm calcitonin muaj cov amino acid sib txawv, tab sis calcitonin los ntawm cov kab mob sib txawv muaj cov haujlwm hauv tib neeg. Ntawm lawv, salmon calcitonin (sCT) muaj qhov ua haujlwm siab tshaj plaws, ua rau nws muaj txiaj ntsig zoo hauv kev siv tshuaj kho mob.
Nyob rau hauv txoj kev loj hlob ntawm salmon calcitonin, Guttmann thawj synthesized salmon calcitonin nyob rau hauv 1969, uas muab ib tug muaj peev xwm rau cov tom ntej daim ntawv thov ntawm salmon calcitonin.
Tom qab ntawd, ntau qhov kev sim tshuaj thiab cov kev tshawb fawb tau ua kom paub meej tias qhov ua tau zoo thiab kev nyab xeeb ntawm salmon calcitonin hauv kev kho mob pob txha. Salmon calcitonin yog dav siv los tiv thaiv thiab kho cov neeg laus osteoporosis, Paget's syndrome, hypercalcemia thiab lwm yam kab mob, thiab ua cov tshuaj pab kho mob ntshav qab zib, gastric ulcer, mob pancreatitis thiab lwm yam kab mob.
Ntxiv rau salmon calcitonin, tib neeg kuj tau kawm lwm qhov chaw ntawm calcitonin, xws li eel calcitonin thiab tib neeg calcitonin. Eel calcitonin yog cov qauv zoo ib yam li cov ntses salmon calcitonin, tab sis nws cov kev ua haujlwm lom neeg kuj tsawg. Txawm hais tias tib neeg calcitonin txawv ntawm salmon calcitonin, nws cov txheej txheem ntawm kev ua thiab kev ua haujlwm zoo sib xws.
Cov tshuaj no yog polypeptide hormone secreted los ntawm salmon thyroid follicular hlwb. Txij li thaum nws qhov kev tshawb pom, nws tau txais kev saib xyuas thoob plaws hauv thaj chaw ntawm cov tshuaj vim nws qhov tshwj xeeb lom neeg ua haujlwm thiab dav siv tshuaj kho mob tus nqi. Nrog rau kev laus zuj zus ntawm cov pej xeem thoob ntiaj teb, qhov tshwm sim ntawm hnub nyoog - cuam tshuam txog pob txha, Paget's kab mob thiab lwm yam kab mob tau nce xyoo los ntawm xyoo. Raws li cov tshuaj muaj txiaj ntsig zoo los kho cov kab mob no, salmon calcitonin kev lag luam xav tau thiab kev loj hlob zeem muag tau nce zuj zus. Nov yog nws txoj kev loj hlob zeem muag:
Kev ua lag luam xav tau kev txheeb xyuas
Osteoporosis kev lag luam
Osteoporosis yog ib qho kab mob ntawm cov pob txha uas ua rau cov pob txha loj zuj zus, kev puas tsuaj rau cov pob txha, cov pob txha muaj zog, thiab muaj kev pheej hmoo ntawm pob txha. Nrog rau kev laus zuj zus ntawm cov pej xeem thoob ntiaj teb, qhov tshwm sim ntawm cov pob txha pob txha tau nce xyoo los ntawm xyoo, uas tau dhau los ua teeb meem kev noj qab haus huv thoob ntiaj teb. Nws tau kwv yees tias kwv yees li 200 lab tus tib neeg thoob ntiaj teb raug kev txom nyem los ntawm pob txha, nrog rau feem ntau ntawm cov poj niam cov neeg mob. Salmon calcitonin yog ib qho ntawm cov tshuaj siv tau zoo rau kev kho mob pob txha, thiab nws cov kev xav tau ntawm kev lag luam tau nce ntxiv nrog kev nce ntawm qhov tshwm sim ntawm cov pob txha.
Paget tus kab mob ua lag luam
Paget tus kab mob yog ib qho mob pob txha metabolism tsis zoo uas tshwm sim los ntawm kev loj hlob ntau dhau thiab kev hloov pauv ntawm cov pob txha tsis zoo. Tus kab mob no tshwm sim hauv Tebchaws Europe thiab Tebchaws Meskas, tab sis nrog kev siv zog ntawm cov neeg thoob ntiaj teb kev txav mus los thiab kev laus, nws qhov xwm txheej tau maj mam nce ntxiv hauv lwm cheeb tsam. Salmon calcitonin ua lub luag haujlwm tseem ceeb hauv kev kho mob ntawm Paget tus kab mob, txo cov pob txha mob, txo cov ntshav calcium, thiab txhim kho pob txha zoo. Yog li ntawd, nrog rau qhov nce ntawm qhov tshwm sim ntawm Paget tus kab mob, kev ua lag luam thov rau salmon calcitonin yuav nce raws li.
Kev ua lag luam rau hypercalcemia
Hypercalcemia yog hais txog lub xeev pathological uas qhov concentration ntawm calcium ions nyob rau hauv cov ntshav yog txawv txav, uas tuaj yeem tshwm sim los ntawm ntau yam xws li hyperparathyroidism, malignant qog pob txha metastasis, thiab lwm yam. Hypercalcemia tuaj yeem ua rau ntau lub cev puas tsuaj thiab tuaj yeem ua rau lub neej - hem nyob rau hauv mob hnyav. Salmon calcitonin, uas yog ib qho ntawm cov tshuaj zoo rau kev txo cov ntshav calcium, ua lub luag haujlwm tseem ceeb hauv kev kho mob hypercalcemia. Nrog rau kev nkag siab tob ntawm hypercalcemia thiab kev nce qib ntawm txoj kev kho mob, qhov kev thov rau salmon calcitonin hauv kev ua lag luam hypercalcemia yuav loj hlob ntxiv.
Kev ua lag luam kev sib tw qauv
Kev sib tw ua lag luam hnyav
Tam sim no, kev sib tw hauv kev lag luam salmon calcitonin yog qhov hnyav, nrog ntau lub tuam txhab tshuaj nkag mus rau hauv daim teb no. Cov tuam txhab lag luam no sib tw ua lag luam sib koom ua lag luam raws li cov khoom lag luam zoo, tus nqi, kev pabcuam, thiab lwm yam. Txhawm rau kom sawv tawm hauv kev sib tw, cov lag luam yuav tsum tau ua kom zoo dua cov khoom lag luam thiab qib thev naus laus zis, ntxiv dag zog rau kev tsim khoom lag luam thiab kev lag luam.
Patent tiv thaiv thiab kev sib tw tshuaj generic
Salmon calcitonin, ua ib qho tseem ceeb peptide tshuaj, muaj lub sij hawm txwv patent tiv thaiv. Raws li lub sijhawm tiv thaiv patent tas sij hawm, cov tshuaj generic yuav maj mam nkag mus rau hauv kev ua lag luam thiab sib tw nrog cov tshuaj qub. Qhov no yuav ua rau txo qis ntawm cov nqi lag luam thiab kev hloov pauv hauv kev sib tw toj roob hauv pes. Yog li ntawd, thawj cov tuam txhab tshuaj yuav tsum ntxiv dag zog thev naus laus zis tshiab thiab lub tsev lag luam kom muaj kev sib tw kom zoo dua hauv kev sib tw tshuaj generic.
Kev lag luam thoob ntiaj teb kev sib tw
Nrog rau kev sib sib zog nqus zuj zus thiab kev loj hlob ntawm kev lag luam thoob ntiaj teb, kev lag luam salmon calcitonin tseem tab tom ntsib kev sib tw los ntawm kev sib tw thoob ntiaj teb. Cov tuam txhab txawv teb chaws muaj qee qhov zoo hauv kev siv tshuab, peev, hom thiab lwm yam, uas ua rau muaj kev sib tw siab rau cov lag luam hauv tsev. Txhawm rau tiv thaiv kev sib tw thoob ntiaj teb, cov lag luam hauv tsev yuav tsum muaj kev sib koom tes thiab kev sib txuas lus nrog cov lag luam thoob ntiaj teb, sib koom ua ke tsim cov khoom tshiab, tshawb nrhiav cov lag luam tshiab, txhim kho cov khoom zoo, thiab txo cov nqi.
Cov lus nug nquag
Puas yog calcitonin salmon tshuaj tsuag qhov ntswg raug txiav?
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US Food and Drug Administration (FDA) tshaj tawm kev txiav tawm ntawm calcitonin -salmon [rDNA keeb kwm] (Fortical) tshuaj tsuag qhov ntswg. Upsher-Smith Laboratories txiav txim siab txiav kev tsim cov khoom, raws li tsab ntawv ceeb toom FDA.
Puas yog calcitonin salmon tshuaj tsuag muaj kev nyab xeeb?
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Hauv kev tshawb fawb soj ntsuam, ntau tus neeg siv calcitonin salmon tsim mob qog noj ntshav dua li cov neeg siv cov tshuaj placebo. Tham nrog koj tus kws kho mob yog tias koj muaj lus nug txog qhov kev mob tshwm sim no. Lawv tuaj yeem tham txog cov txiaj ntsig thiab kev pheej hmoo ntawm cov tshuaj no rau koj.
Cim npe nrov: Calcitonin Salmon hmoov CAS 47931-85-1, lwm tus neeg, manufacturers, hoobkas, lag luam wholesale, yuav, nqe, tej, kev muag khoom