Indolicidinyog luv peptide nrog cov tshuaj tua kab mob, koom nrog ib hom tshuaj tiv thaiv kab mob ntuj peptide. Ua los ntawm 13 amino acid residues, cov amino acids txuas ua ke los ntawm peptide daim ntawv cog lus los tsim cov saw hlau peptide. Hauv kev daws teeb meem, nws nthuav tawm helical conformation uas pab txhawb nws kev sib cuam tshuam nrog lwm cov molecules lossis cellular qauv. Vim nws qhov tshwj xeeb amino acid muaj pes tsawg leeg thiab ib ntus, nws tuaj yeem xaiv khi rau lub hom phiaj hauv cov kab mob, yog li siv nws cov tshuaj tua kab mob.
Nyob rau hauv cov xwm txheej tsim nyog, nws tuaj yeem tswj tau qhov ruaj khov ntawm nws cov qauv thiab kev ua haujlwm. Txawm li cas los xij, ntawm qhov kub siab dua, nws cov ntawv cog lus peptide tuaj yeem cuam tshuam, ua rau kev hloov pauv ntawm cov qauv thiab tsis ua haujlwm. Yog li ntawd, hauv kev tshawb fawb thiab kev siv, nws yog ib qho tsim nyog los tswj qhov kev sim ntsuas kub kom ntseeg tau tias nws ruaj khov thiab ua haujlwm. Raws li ib tug natural antimicrobial peptide, nws muaj ntau yam haujlwm xws li tshuaj tua kab mob, tiv thaiv kab mob, tiv thaiv- qog, thiab tshuaj tua kab mob.
Customized Bottle Caps & Corks
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Indolicidin COA
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Tshuaj Formula |
C100H132N26O13 |
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Exact Mass |
1905 |
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Molecular Luj |
1906 |
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m/z |
1906 (100.0%), 1905 (92.5%), 1907 (39.5%), 1907 (14.0%), 1908 (10.8%), 1907 (9.6%), 1906 (8.9%), 1908 (7.2%), 1909 (4.3%), 1908 (3.8%), 1908 (2.7%), 1907 (2.5%), 1907 (1.5%), 1909 (1.4%), 1906 (1.4%), 1908 (1.3%), 1909 (1.0%) |
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Elemental Analysis |
C, 63.01; H, 6.98; N, 19.10; O, 10.91 |
antibacterial kev ua haujlwm
Indolicidinnthuav tawm dav -spectrum antibacterial kev ua haujlwm tawm tsam ntau yam kab mob, suav nrog ntau yam aerobic Gram-cov kab mob tsis zoo, Gram- cov kab mob zoo, thiab cov kab mob fungi. Qhov dav spectrum ntawm kev ua ub no ua rau nws yog ib tus neeg sib tw rau kev kho mob antimicrobial.
Membrane Permeabilization
Cov qauv tshuaj tshwj xeeb, nplua nuj nyob rau hauv tryptophan residues thiab nrog ib qho amidated carboxyl terminus, tso cai rau nws nkag mus rau cov kab mob cell membranes thiab cuam tshuam lawv kev ncaj ncees. Qhov no ua rau efflux ntawm cov kab mob, thaum kawg ua rau cov kab mob tuag.
DNA Binding thiab Inhibition ntawm Replication
Cov kev tshawb fawb tau pom tias nws tuaj yeem khi rau ob npaug -helical DNA, tejzaum nws inhibiting ntau cov enzymes ntsig txog DNA ua. Tshwj xeeb, PW-WP structural motif nyob rau hauv nruab nrab ntawm lub kaum peb-peptide yog ib tug tshwj xeeb structural element uas muaj peev xwm envelop lub B-daim DNA ob helix, txhim khu nws stability thiab inhibiting DNA replication thiab transcription.
Kev sim hauv vitro tau pom tias nws muaj peev xwm tua cov kab mob siab, nrog ze rau - tua tag nrho cov kab mob sib kis xws li Escherichia coli thiab Staphylococcus aureus. Qhov kev ua tau zoo no qhia txog nws lub peev xwm ua tus neeg sawv cev antimicrobial.
Kev txwv & Derivative Development
Txawm hais tias nws cov tshuaj tua kab mob tseem ceeb, daim ntawv thov kev kho mob tsuas yog txwv vim nws cytotoxicity rau cov qe ntshav liab, uas tuaj yeem ua rau hemolysis yooj yim.
Tsis tas li ntawd, nws txoj kev sib raug zoo siab rau kev khi rau saum npoo lipopolysaccharides, thaum pab txhawb rau nws cov tshuaj tua kab mob, kuj tseem tuaj yeem ua rau kev txhim kho tshuaj tiv thaiv hauv qee yam kab mob.
Txhawm rau kov yeej cov kev txwv no, cov kws tshawb fawb tau tsim ntau yam khoom siv thiab analogs.
Piv txwv li, Indolicidin acetate thiab Indolicidin TFA yog cov derivatives tau los ntawm kev hloov kho tshuaj uas tswj xyuas cov kab mob tua kab mob thaum txo cov tshuaj lom rau cov qe ntshav liab. Tsis tas li ntawd, qee cov kev tshawb fawb tau sim txhim kho cov kab mob tua kab mob thiab txo cov cytotoxicity los ntawm kev hloov cov amino acid sib lawv liag, xws li X-, uas yog oxidized nyob rau hauv ob qho tib si ntawm cov tshuaj indole. thiab khaws cia tib yam amino acid ib ntus thiab kev ua haujlwm tua kab mob thaum tiv thaiv proteases.
Antiviral teebmeem
Indolicidinkuj qhia tau hais tias cov tshuaj tiv thaiv kab mob, tshwj xeeb tshaj yog tawm tsam qee yam kab mob xws li HIV-1 thiab tus kab mob herpes simplex. Nws cov tshuaj tiv thaiv kab mob sib txawv me ntsis ntawm nws cov tshuaj tiv thaiv kab mob. Cov kev tshawb fawb tau pom tias nws tuaj yeem khi ncaj qha rau DNA, cuam tshuam nrog kev tsim cov kev sib xyaw ua ke-DNA complexes tsis yog khi rau kev sib koom ua ke nws tus kheej. Qhov kev khi no tuaj yeem cuam tshuam ntau yam enzymes cuam tshuam nrog DNA ua, yog li inhibiting viral replication.
HIV-1 Inhibition
Nws tau pom tias inhibit HIV-1 replication zoo. Cov tshuaj tiv thaiv kab mob xav tau ntau dua li qhov ua rau cov kab mob tua kab mob thiab tshuaj tua kab mob.
Piv txwv li, qhov concentration ntawm 333 ug / ml tuaj yeem tiv thaiv HIV-1, nrog rau IC50 tus nqi xws li 67 txog 100 ug / ml ntawm 37 degree. Kev cuam tshuam rau nws rau 5 feeb ntawm 37℃tuaj yeem cuam tshuam 50% ntawm tus kab mob HIV replication, thaum ua tiav cov tshuaj tiv thaiv kab mob yuav tsum tau kwv yees li 60 feeb.
Mechanism ntawm Action
Kev tshawb fawb qhia tias nws khi rau DNA, tshwj xeeb tshaj yog PW-WP tus qauv tsim nyob rau hauv indolicidin kaum peb-peptide, uas tuaj yeem qhwv ib ncig ntawm B-daim DNA ob lub helix, txhim kho nws ruaj khov thiab inhibiting DNA replication thiab transcription. Qhov kev sib khi muaj peev xwm no yog qhov tseem ceeb rau nws txoj haujlwm tiv thaiv kab mob.
Immunomodulatory teebmeem
Indolicidintau pom tias yuav txhawb lub cev tiv thaiv kab mob, pab lub cev kom tiv thaiv kab mob thiab kab mob. Qhov no yog vim nws lub peev xwm los cuam tshuam nrog lub cev tiv thaiv kab mob thiab txhawb nqa lawv kom muaj zog tiv thaiv.
Cytokines yog qhov taw qhia cov molecules uas ua lub luag haujlwm tseem ceeb hauv kev tiv thaiv kab mob. Nws tuaj yeem cuam tshuam rau kev tsim cov cytokines, xws li interleukins thiab chemokines, uas koom nrog hauv orchestrating lub cev tiv thaiv kab mob.
Innate immune cells, xws li neutrophils, macrophages, thiab natural killer (NK) cells, yog thawj kab ntawm kev tiv thaiv kab mob. Nws tuaj yeem txhim kho cov haujlwm ntawm cov hlwb no, ua rau lawv muaj txiaj ntsig zoo ntawm kev tua cov kab mob lossis tsom mus rau cov hlwb txawv txav.
Txawm hais tias qhov cuam tshuam ncaj qha rau kev tiv thaiv kab mob (T thiab B cov lus teb ntawm tes) tsis tau kawm ntau, nws lub peev xwm los hloov kho cov tshuaj tiv thaiv kab mob hauv lub cev tuaj yeem cuam tshuam tsis ncaj qha rau kev tiv thaiv kab mob.
Ntxiv nrog rau nws cov tshuaj tiv thaiv kab mob ncaj qha, nws kuj muaj cov tshuaj tiv thaiv -cov khoom ua rau mob. Qhov no yog qhov tseem ceeb hauv kev tiv thaiv kev mob ntau dhau, uas tuaj yeem ua rau cov ntaub so ntswg thiab lub cev puas tsuaj. Los ntawm kev tswj cov kab mob inflammatory, nws txhawb nqa kev tiv thaiv kab mob homeostasis.
Kev daws zaum kawg
Kev Kho Mob Hauv Kab Mob
Los ntawm kev txhim kho lub cev tiv thaiv kab mob, nws tuaj yeem siv los ua kev kho mob ntxiv los txhim kho kev ua tau zoo ntawm cov tshuaj tua kab mob lossis tshuaj tiv thaiv.
Kev kho mob autoimmune
Los ntawm kev hloov kho lub cev tiv thaiv kab mob, nws yuav muaj txiaj ntsig zoo hauv kev kho mob autoimmune.
Kev Kho Mob Cancer
Txawm hais tias cov teebmeem antitumor ncaj qha tsis tau ua kom pom tseeb, nws cov cuab yeej immunomodulatory tuaj yeem siv tau.
Indolicidinyog ib yam tshuaj tiv thaiv kab mob peptide (AMP) uas muaj 13 amino acids, Ameslikas cais tawm ntawm bovine neutrophils. Nws qhov tshwj xeeb tryptophan (Trp) thiab proline (Pro) qauv endows nws nrog dav -spectrum antibacterial, antiviral thiab antifungal kev ua ub no, nrog rau inhibitory cuam tshuam rau qee cov qog nqaij hlav cancer. Vim nws qhov peev xwm ntawm daim ntawv thov kev kho mob, kev sib xyaw thiab kev tsim cov Indolicidin tau dhau los ua qhov kev tshawb fawb hotspot. Kab lus no yuav piav qhia txog cov ntaub ntawv tsim khoom los ntawm plaub yam: tshuaj synthesis, recombinant nthuav qhia, kev hloov kho kom zoo, thiab cov teeb meem loj hauv - teev ntau lawm.
Tshuaj Synthesis Method
Kev sib xyaw tshuaj lom neeg yog txoj hauv kev tseem ceeb rau kev loj - nplai ntau lawm ntawm Indolicidin hauv chav kuaj, thiab nws yog qhov tshwj xeeb tshaj yog tsim nyog rau kev kawm hloov kho cov qauv.
Solid Phase Peptide Synthesis (SPPS)
Txoj Cai:Raws li Fmoc (fluorene methoxycarbonyl) los yog Boc (tert-butoxycarbonyl) cov tswv yim tiv thaiv, peptide chains tau maj mam sib sau ua ke ntawm cov resin.
Cov kauj ruam:
Resin xaiv:Feem ntau siv resins yog Rink amide resin lossis Wang resin.
Amino acid coupling:13 amino acids txuas nrog hauv ntu (ib ntu: ILPWKWPWWPWRR-NH₂), thiab tryptophan (W) yuav tsum muaj kev tiv thaiv tshwj xeeb (xws li Boc-Trp(Boc)-OH).
Txiav thiab purification:Cov peptide chains raug txiav siv trifluoroacetic acid (TFA), thiab ces purified los ntawm siab -performance kua chromatography (HPLC) (qhov purity feem ntau yog> 95%).
Qhov zoo:Kev hloov pauv tau yooj yim, tso cai rau kev qhia txog cov tsis yog -natural amino acids lossis hloov cov pab pawg.
Qhov tsis zoo:Tus nqi siab, tsis haum rau loj - teev ntau lawm.
Kua-theem Synthesis
Txoj kev no tsim nyog rau kev sib txuas ntawm cov peptide luv luv, tab sis nws tsis tshua siv rau kev sib txuas ntawm Indolicidin.
Recombinant Express Method
Txhawm rau txo cov nqi thiab ua tiav cov khoom loj -, cov kws tshawb fawb tau sim qhia Indolicidin los ntawm microbial lossis cellular systems recombination.
Prokaryotic Express System
Cov kab mob hauv tsev: Escherichia coli (E. coli) yog cov neeg siv ntau tshaj plaws, tab sis cov teeb meem hauv qab no yuav tsum tau hais txog:
Toxicity: Indolicidin muaj cov nyhuv tua ntawm cov kab mob hauv lub cev. Lub sij hawm nthuav qhia yuav tsum tau tswj xyuas nruj me ntsis siv cov neeg txhawb nqa uas tsis muaj zog (xws li T7 lossis araBAD).Solubility: Nws yooj yim los tsim lub cev suav nrog. Fusion cim npe (xws li SUMO, Trx) yuav tsum tau txhim kho kev solubility. Lub hom phiaj peptide tuaj yeem raug tso tawm los ntawm enzymatic cleavage tom qab.Txhim kho qhov zoo: Kev nthuav tawm ntawm qhov kub thiab txias (20-25 degree) ua rau txo qis hauv kev tsim cov khoom siv hauv lub cev.Lub teeb liab peptide (xws li PelB) yog siv los tso cov peptide mus rau hauv qhov chaw periplasmic, yog li txo cov tshuaj lom neeg.
Eukaryotic Express System
Poov xab system (xws li Pichia pastoris):
Qhov zoo: Muaj peev xwm tshaj tawm - kev hloov pauv kev txhais lus, haum rau cov peptides nyuaj.
Kev sib tw: Indolicidin tej zaum yuav degraded los ntawm cov poov xab proteases, yog li yuav tsum tau siv ib tug protease -deficiency strain.
Mammalian hlwb: Tus nqi kim heev, npaj tsuas yog rau kev tshawb fawb.
Kev ua hauv qab
Cell cuam tshuam thiab ntes: Cells cuam tshuam siv ultrasound lossis siab -siab homogenization, thiab fusion protein yog purified los ntawm affinity chromatography.
Enzymatic cleavage thiab purification: Siv enterokinase los yog SUMO protease tshem tawm cov cim npe, thiab tom qab ntawd ua rov qab -theem HPLC purification ntawm lub hom phiaj peptides.
Kev hloov kho thiab kev ua kom zoo
Lub ntuj Indolicidin nthuav tawm hemolytic toxicity thiab proteinase instability, thiab yog li yuav tsum tau hloov kho los txhim kho nws cov kev ua tau zoo.
Amino acid hloov
Txo toxicity: Hloov Arg (R) nrog hydrophobic amino acids (xws li Ala), txo cov kev cuam tshuam nrog mammalian cell membranes.
Txhim khu kev ruaj ntseg: Qhia D-hom amino acids lossis -amino acid tiv thaiv cov proteins kom tiv thaiv kev puas tsuaj ntawm protease.
Lipidation/PEGylation
N-terminal acetylation lossis txuas ntawm polyethylene glycol (PEG) ua kom ntev ib nrab- lub neej.
Heterodipeptide Design
Fusion nrog lwm cov AMP fragments (xws li LL-37) los txhim kho kev ua si lossis nthuav dav cov kab mob tua kab mob.
Cov kev sib tw ntawm Loj- teev ntau lawm
Tus nqi tswj
Kev siv tshuaj lom neeg: Tus nqi ib gram tuaj yeem ncav cuag ntau txhiab daus las, uas tsis haum rau kev kho mob xav tau.Reorganization ntawm nthuav qhia: Cov txheej txheem fermentation yuav tsum tau ua kom zoo dua qub (xws li los ntawm kev cog qoob loo siab -) kom ntau lawm.
Kev Tswj Xyuas Zoo
Cov kev cai huv huv: Cov tshuaj peptides yuav tsum ua raws li FDA / EMA cov qauv ( impurities <0.1%).Kev soj ntsuam kev ua haujlwm: Qhov tsawg kawg nkaus inhibitory concentration (MIC) thiab hemolysis test (HC₅₀) yog cov cim tseem ceeb.
Kev cai thiab kev nyab xeeb
Nws yog ib qho tsim nyog los ua qhov kev ntsuam xyuas tshuaj toxicological preclinical, tshwj xeeb tshaj yog rau immunogenicity thiab ntev - toxicity.
Yav tom ntej Outlook
Cell-kev sib txuas pub dawb: Ua tau sai sai siv qhov kev txhais lus hauv vitro, tsis txhob muaj tus tswv tsev toxicity.Nanocarrier xa: Txhim kho lub hom phiaj los ntawm kev sib txuas liposomes lossis polymers.
AI-pab tsim: Kev kwv yees ua tau zoo thiab qis dua - lom sib txawv los ntawm kev kawm tshuab.
Kev tsim tawm ntawmtshuaj indolicidinyuav tsum muaj qhov sib npaug ntawm qhov tseeb ntawm cov tshuaj synthesis thiab scalability ntawm recombinant》thiab nanotechnology), nws daim ntawv thov kev kho mob yuav tsum tau nce.
FAQ
Indolcidin yog ib hom tshuaj tiv thaiv kab mob ntuj peptide uas muaj qhov dav dav -spectrum antibacterial kev ua haujlwm. Nws tuaj yeem cuam tshuam zoo thiab tshem tawm ntau yam gram- cov kab mob zoo, gram- cov kab mob tsis zoo thiab cov kab mob ib nrab, thiab tseem muaj qee yam kev tiv thaiv kab mob - mob. Nws tuaj yeem cuam tshuam qhov kev tso tawm ntawm cov kab mob inflammatory, txo cov kab mob hauv zos, thiab tswj cov kab mob physiological ruaj khov.
Nws muaj kev nplua nuj daim ntawv thov tus nqi hauv ntau qhov kev lag luam. Nws tuaj yeem siv tau raws li qib siab -cov khoom siv tshuaj tua kab mob zoo rau tsiaj pub ntxiv kom txo tau cov tsiaj nyeg thiab nqaij qaib. Tsis tas li ntawd, nws tseem tuaj yeem siv rau hauv txhua hnub tshuaj antisepsis, kev tshawb fawb biomedical thiab cov khoom siv kho mob sab nraud, ua si muaj kev nyab xeeb thiab ruaj khov tiv thaiv kab mob thiab tiv thaiv lub luag haujlwm.
Sib txawv ntawm cov tshuaj bacteriostatic cov tshuaj, Indolcidin nta cov tshuaj tiv thaiv tsis tshua muaj kev pheej hmoo thiab zoo biocompatibility. Nws ua sai sai ntawm cov kab mob pathogenic microorganisms nrog kev ua tau zoo me me, yuav tsis yooj yim ua rau cov ntaub so ntswg ua rau lub cev tsis zoo, thiab muaj cov thermal stability thiab acid- puag kam rau ua, uas yooj yim rau kev ua cov qauv thiab ntev - lub sij hawm cia hauv ntau yam kev npaj.
Cim npe nrov: Indolicidin CAS 140896-21-5, lwm tus neeg, manufacturers, hoobkas, lag luam wholesale, yuav, nqe, tej, kev muag khoom