DL-dithiothreitol cas 3483-12-3

Dl-dithiothreitol(DTT rau Luv luv) yog cov molecule me me uas txo tus neeg saib xyuas nrog cov kab mob sib txawv ntawm lub xeev txo qis, thiab ua tus tswv cuab tam sim no monosaccharide . Tus isomer ntawm DithioRYRYRY), uas yog C3}} soluble hauv dej {}

Vim tias nws yooj yim los ua oxidized los ntawm huab cua, qhov ruaj khov ntawm DTT yog neeg pluag; Txawm li cas los xij, khov cia lossis kho cov roj hauv cov roj inert {}} kev tawm tsam sulfur, kev txo qis ntawm DTT txo qis ntawm PH; Tris ({2- carboxyethethyl) posp hydrockyloride) tuaj yeem siv los hloov rau dtt .

DL Dithiothreitol (DTT) yog cov tshuaj tua tsiaj me me, cov tshuaj tiv thaiv kab mob, kev ua haujlwm hauv ib puag ncig, ua liaj ua teb thiab lwm cov liaj teb.

Biotechnology Fid

 

Protein lysis thiab disulfide txo qis:
Cov ntawv thov tseem ceeb ntawm DTT hauv biotechnology yog li protein lysees {}} Txoj Kev Khomob (COr) Kev Tshawb Fawb Txog Cov Proteins {{4 4}} Piv txwv li cov protein discacation kom ntseeg tau tias muaj protein disein sib cais ua ke, ntawm cov protein sib cais .
Txoj kev ceev ntawmDl-dithiothreitol is significantly affected by pH value and only works when pH>{{07. Qhov no yog vim tias tsuas yog protonated thiol spects yog feem ntau siv nyob rau hauv alkaline tej xwm txheej kom ntseeg tau tias nws txo kev ua haujlwm .

 

DNA synthesis thiab kev hloov kho
DTT kev ua raws li kev txo qis thiab tsis txaus ntseeg hauv DNA lub davhlau ya nyob rau hauv kev daws teeb meem, hauv DNA cov tshuaj tsw qab, uas tuaj yeem txo cov DNA DNA poob kev thiab txhim kho kho Kev ua tau zoo . Tsis tau siv los tshem tawm cov pab pawg tiv thaiv thaum lub sijhawm ua suab paj nruag {}}
Siv Kev Pab Siv RNA
DTT muaj cov nyhuv inhibitia ntawm RNA enzyme kev ua si hauv cov qauv rna thiab inactivating deque protisins. Piv txwv, hauv RNA cov tsev qiv ntawv siv thiab Amplification kev sim, ntxiv rau ntawm DTT tuaj yeem txhim kho RNA tus nqi kev ncaj ncees thiab sim ua tiav txoj kev vam meej.

Biomedical teb

 

Tshuaj tiv thaiv cov tshuaj tiv thaiv thiab nucleic acid kuaj:
DTT ua raws li cov protein thiol tiv thaiv, tiv thaiv cov tshuaj tiv thaiv kab mob intramolecular, nyob rau hauv kev tsim cov kev sib xyaw ua ke, DTT tau siv los ua kom zoo dua qhov ruaj ntseg ntawm antigen Protein .
Nyob rau hauv nucleic acid kuaj, tseem siv rau inhibit rnase kev ua si thiab xyuas kom muaj cov protein tawm thaum lub sijhawm nucleic acid extraction rau nucleic acid purity acid purity {}}

 

Detoxification ntawm Cellular Cov Ntaubmob thiab Hluav Taws Xob Hluav Taws Xob:
DTT muaj Antioxidant thaj chaw thiab tuaj yeem tiv thaiv cov hlwb thiab cov ntaub so ntswg ua haujlwm rau kev kho hluav taws xob los ntawm kev kho hluav taws xob los ntawm kev kho hluav taws xob los ntawm kev siv tshuaj lom neeg
Khob Ntshav Ntshav Liab thiab Blood Pej Xeem:
DTT ua si lub luag haujlwm tseem ceeb hauv kev kho ntshav liab {{}}} Cov tshuaj tiv thaiv kab mob cd38 monoclonal tshuaj tiv thaiv kab mob CD38 monoclonal Antibody arti}} DTT tseem tuaj yeem rhuav tshem cov ntawv disulfide ntawm cov beackantibody ib

Tshuaj Kho Mob Daim teb

 

Enzyme kev tiv thaiv thiab inhibitors
DTT tuaj yeem tiv thaiv cov pab pawg txo ntawm enzyme molecules, thiab txhim kho enzyme, thiab siv tau cov txiaj ntsig zoo, thiab ua kom muaj zog ntxiv, hauv kev sib txawv, hauv kev ua kom ruaj khov, thiab ua kom muaj zog ntxiv, hauv kev ua kom ruaj khov, thiab siv tau rau cov enzymatic ntawm enzymes rau kev kawm lawv cov qauv ntawm kev ua {{2 2}}
Ntsuas enzyme kev ua si
DTT yog ib qho tseem ceeb hauv enzyme kev txiav txim siab .} Kuv tuaj yeem koom nrog enzymatic cov kev ua si .

Kev Tiv Thaiv Ib Puag Ncig Teb

 

Wastewater kev kho thiab cov kuab paug:
Dl-dithiothreitoltuaj yeem siv hauv kev kho dej kom tshem tawm cov hlau hnyav thiab cov tshuaj lom neeg muaj kuab lom, yog li muaj cov tshuaj lom thiab muaj tshuaj tua kab mob {}}
Synthesis ntawm kub nanomaterials:
DTT ua si lub luag haujlwm tseem ceeb hauv cov hluavtaws kub nanomaterials {}} Cov ntaub ntawv txo qis, nyob rau hauv cov khoom siv tsawg, xws li Quantum dots thiab nanowires .

Khoom Noj thiab Cov Tshuaj pleev Kev Lag Luam

 

Antioxidants thiab tshuaj tiv thaiv:
DTT muaj antioxidant thiab tiv thaiv cov teebmeem hauv cov zaub mov ntxiv thiab kev puas tsuaj rau cov khoom noj uas nquag los ntawm kev ua zaub mov oxidating thiab txhim kho cov khoom ruaj khov.
Khoom Noj Khoom Haus Kev Txhim Kho Zoo:
DTT kuj tseem siv tau rau kev txhim kho cov zaub mov zoo {{}} Kuv tuaj yeem ua rau cov nqaij ua noj, dtt ntawm cov mov ci, txhim kho cov nqaij mov, txhim kho cov nqaij thiab cov kua txiv thiab kua qaub ntawm cov nqaij.

Ua liaj / teb thiab Kev Lag Luam

 

Cov ntawv thov kev ua liaj ua teb:
DTT muaj peev xwm thov cov ntawv thov hauv kev ua liaj ua teb {{}}}}} paab cov tshuaj tua kab mob protein thiab kev ruaj khov thiab ua tau zoo ntawm cov tshuaj tua kab.
Muaj cov khoom siv hluav taws xob thiab cov tshuaj txhua hnub:
DTT tuaj yeem siv rau kev kho cov khoom siv hlau saum npoo av, txhim kho cov khoom siv tshuaj tsw qab, DTT thiab cov khoom siv kom ua txhua yam thiab cov tshuaj tsis huv thiab cov tshuaj tsis huv thiab cov khoom siv.}}

Rau cov qauv hluav taws xob tshiab ntawm cov dithiothreitol, cov khoom siv khoom siv raw yog suav raws li cov khoom lag luam theoretical tawm ntawm 277.6g, suav nrog cov kauj ruam hauv qab no:

.}}}}}}}}}}}} {} {(1.80m) 2, {3- dibromo -1},4- butanediol. Khaws nws rau hauv lub tank nrog qhov ntsuas kub qis dua 30℃rau standby;

B . Nyob rau hauv supernatant tau nyob rau hauv kauj ruam ib tug, thiab cov tshuaj tiv thaiv tau muab tso rau hauv ib qho kub tsawg dua 20℃kom tau txais urylene oxide;

c .}}}}}} ntxiv 155.67g (dokoob

d . Nyob rau hauv lub crystallization tau txais nyob rau hauv kauj ruam c, demreded rau cov tshuaj tiv thaiv ntawm 3%, muab rho tawm nrog 300ml ntawm Ethyl acetate, ua ke ntawm cov txheej txheem organic, distilled nyob rau hauv lub zog siab rau kev txo thiab txias rau chav tsev ething ntawm 18g ntawm dawb tawv ditiothreitol nrog cov txiaj ntsig ntawm 36.02%.

Dl-dithiothreitolyog tus neeg sawv cev muaj zog, thiab nws txoj kev txo qis vim yog kev cuam tshuam nrog kev sib cais ntawm lub ntsej muag (muaj kev sib cais kev tawm tsam .

Ntawm lawv, lub xeev nrab tau tsim nyob rau hauv thawj kauj ruam ntawm cov kev tawm tsam yog heev hloov pauv nrog kev txo qis ntawm disulfide daim ntawv cog lus .

Lub zog txo qis ntawm DTT cuam tshuam los ntawm tus nqi pH uas yog ntau dua {} Qhov no yog vim tias tsuas yog muaj ntau dua {( PKA ntawm Thiol pab pawg yog feem ntau ~ 8.3.

Vim tias nws yooj yim los ua oxidized los ntawm huab cua, qhov ruaj khov ntawm DTT yog neeg pluag; Txawm li cas los xij, khov cia lossis kho cov roj hauv cov roj inert {}} kev tawm tsam sulfur, kev txo qis ntawm DTT txo qis ntawm PH; Tris ({2- carboxyethethyl) posp hydrockyloride) tuaj yeem siv los hloov rau dtt .

Cim npe nrov: DL-Dithiothreitol Cas {{}}}, cov chaw muag khoom, chaw tsim khoom, Hoobkas, yuav, nqe, loj, rau muag