Fluoresinin isothiocyanate ua 27072-45-3

Fluoresin isothiocyanatetxiv kab ntxwv liab, othocyanate fluorescent daj, othotiocyanate fluorescein isomer i6}} nws tuaj yeem khi nrog ntau cov tshuaj tiv thaiv antibody, thiab cov antibody tom qab khi tsis tau poob qhov tshwj xeeb ntawm kev sib khi nrog cov tshuaj ntsuab ploj mus,} Nws tseem muaj zog} nws tseem muaj zog ntsuab, thiab cov roj av {{} nws yog biochemical reagent nrog rau kev kuaj tshuaj kho mob, uas tuaj yeem khi cov tshuaj tiv thaiv ntau yam ntxiv, thiab muaj ntau yam kev ua kom muaj zog ntxiv rau cov tshuaj tua kab mob alkaline} precipitates, thiab fluorescence ploj, thiab nws yog me ntsis soluble hauv acetone, etherum {{10} {10}

Kawm txog cov yam ntxwv ntawmfluoresin isothiocyanateSau npe insulin rau aminated gelatin thiab nws daim ntawv thov hauv Nasal Mucosa Administration:

Nyob rau hauv cov xwm txheej ntawm tsis muaj emulsifier sab nraud, cov oveasing gel rub tawm, thiab kev sib tw ua kom zoo nkauj thiab kev sib tw nruab nrab yog} {} m . Qhov loj yog tusyees faib tawm ntawm cov dej hydrogpheres tau ua rau muaj cov dej khov kho thiab cov tshuaj phosphate dolution, thiab cov Zata muaj peev xwm nyob rau hauv dej distilled yog 41.} 5 MV.

Nrog rau qhov nce ntawm glutaraldehyde concentration lossis txuas ntxiv ntawm cov kev xav tsis meej, tsis hais cov tshuaj tiv thaiv gelatin ntxiv (<1h) in the presence of trypsin, but slowly (>20H) hauv Pepsin thiab Acid-Line Nruab Nrab. Qhov kev cuam tshuam zoo tshaj plaws ntawm cov nyom gelatin {4 4}} lub roj nirodesive ntawm aminated gelatin jookheres tau kawm los ntawm fluorescence daim ntawv thiab hauv vitro enzyme tsis kam ua qauv thiab hauv vivo kev sim hauv cov nas.

Cov txiaj ntsig tau pom tias nyob rau hauv tib qho kev sim ua ke, tus naj npawb ntawm cov kua dej tsis sib xws, thiab cov tshuaj pleev plab zom mov (ensificial kev sib cuam tshuam ntawm lub microspheres thiab lub plab zom mov thiab lub plab zom mov Tsis yog txiav txim siab rau lub plab kom rov qab ntawm lub micreghughetes gelatin rau ntawm cov kev sim ua kom zoo nkauj, nws tau pom tias cov roj hmab ua kom pom tseeb, nws tau qhia tias cov roj ntsha ua kom pom tseeb ntawm cov Aminated Gelatin Jearpheres yuav cuam tshuam los ntawm cov amino cov ntsiab lus nyob rau saum npoo ntawm lub jackpheres lossis qib crosslinking .

Kev sim hauv nas hauv nas pom tias amines-{}}}}}} 2% (WN) AMino Gelatin tuaj yeem txhawb kev nqus tau cov tshuaj fluorescein isothonocyanat glucan los ntawm cov nas lub qhov ntswg ntawm 23 {} 8%. Tom qab kev tswj hwm intranasal ntawm 10 iu insulin phosphate Buffer muaj {% (w / v) theem ntshav tau qis dua li insulin phosphate tsis.

Fluoresin isothiocyanate(Fitc), raws li ib qho cuab yeej fluorescent zoo nkauj, tau dhau los ua cov khoom siv tsis tau, thiab cov ntaub ntawv zoo nkauj vim nws cov ntaub ntawv zoo, thiab cov tshuaj ntsuab zoo .

Biomolecular Cim: Txheeb xyuas raws cov protein rau nucleic acids

 

Cov pab pawg ayothocyanananananate pab pawg (- n= Tus cwj pwm uas haum rau cov protein ntau ntxiv rau cov protein ntau ntxiv rau cov protein, nucleic acid, thiab peptide Labeling .
Protein labing thiab kev tshawb nrhiav haujlwm ua haujlwm
Fitc ua tiav cov cim tshwj xeeb los ntawm kev siv cov protein, fluorofluorescence {}}} Cov ntaub ntawv sim qhia tau hais tias tus fluorescence kev siv ntawm Fitc IGG conjugate yog linearly muaj cov fluein kev sib txuas lus hluav taws xob hloov chaw (khov kho) tech.

 

Nucleic acid labeling thiab molecular hybridization
Fitc tuaj yeem raug hloov kho kom sau npe rau 5 'lossis 3' kawg ntawm cov kab mob fluorical (ntses) {{3} {3} Bcr-abl fusion noob tuaj yeem tso cov paib fluorict cov paib hauv nruab nrab ntawm nruab nrab ntawm 95% .
Peptide labeling thiab kev xa tshuaj
FITC sau npe Peptides ua lub luag haujlwm tseem ceeb hauv kev ua yeeb yam, uas muaj peev xwm lees paub cov qog ntawm M2PEP-fitc daim ntawv locomes liposomes hauv Qum cov leeg yog 3 {{{. 2 zaug ntawm cov tshuaj pub dawb, txhim kho kev siv tshuaj kho mob thiab txo cov kev mob tshwm sim.

Kev kuaj mob: Los ntawm cov pevogen nrhiav kom tau thaum ntxov ntawm kev kuaj mob qog

 

Fluorescence cov khoom ntawm Fitc ua nws tus qauv "Kub" hauv kev kos duab ntawm cov tswv yim, ntws cytometry, thiab elisa .
Nrhiav kom ceev ceev ntawm pathogens
FITC daim ntawv lo rau cov tshuaj tiv thaiv kab mob tuaj yeem siv rau cov kab mob fluorescence los, hauv Malaria cov ntshav khi rau ntawm 2 teev Cov hau kev mus rau 15 feeb, thiab qhov rhiab txog 98%. Cov cim acid probent kuj tseem siv tau, haum rau ntawm 99 . 2%.

 

Qaum Cim Cim
Nyob rau hauv n nwg cytometry,fuorescein isothiocyanateDaim ntawv lo lus tawm tsam CD45 tuaj yeem paub qhov txawv ntawm cov leeg ntshav ntawm cov qog ntshav ...} Cov qog nqaij hlav (CTC) yuav ntes cov qog ntshav hauv cov qog mis tsis tshua muaj cancer thiab mob ntsws {}}
Kev kuaj mob ntawm autoimmune kab mob
Nyob rau hauv elisa technology, fitc sau pib Autantibodies (xws li anti lupus {% thiab muaj feem cuam tshuam ntawm 95% rau kev kuaj pom ntawm anti sm tivtib, uas yog ho tsis zoo dua li ib txwm muaj impunofluorescence .

Kev Tshawb Fawb Biology Cell: Los ntawm cov qauv subcellular kom dessaming taug kev

 

The cell permeability and low toxicity of FITC make it an ideal tool for cell imaging, widely used in organelle localization, cell division observation, and apoptosis research.
Cell Orgelelle Labelling thiab Dynamic Kev soj ntsuam
FITC sau npe Mitochracker ntsuab) tuaj yeem tso tawm cov tshuaj tiv thaiv microchivy, fitch} Txoj kev txhim kho morphaological, qhia lub tshuab ntawm neural Kev loj hlob . Txuas ntxiv, LyscTualation Lysosome probes (Lysotracker ntsuab) kuj tseem tuaj yeem siv los ntawm kev hloov pauv ntawm fluorescence {}}

 

Lub voj voog ntawm tes thiab kuaj mob hauv lub agoptosis
Nyob rau hauv cov xim cytometry, FitC sau cov nqi hluav taws xob uas muaj cov txheej txheem sib txawv ntawm cov qauv ntawm Fitc-PI ob zaug Tranpan Blue Stain Txuj . Ntxiv rau cov duab phosexin v probs, ua tiav cov cim ntawm lub cev apoptotic {}}
Kev Tshawb Fawb ntawm Cell tsiv teb tsaws thiab kev txeeb chaw
Hauv kev sim transwell, ntsuas phoo ntawv lochaw cov txheej txheem tsiv teb tsaws, ua kom muaj peev xwm ua tau zoo, muab cov khoom siv tsiv teb tsaws, muab cov khoom siv tsis sib haum xeeb, muab cov khoom siv tsis sib haum xeeb tshuaj .

Kev Tsim Tshuaj Yeeb: Los ntawm phiaj tau lees paub rau kev tshuaj xyuas kev ua tau zoo

 

Fitc ua ntau lub luag haujlwm hauv kev tsim cov tshuaj, suav nrog kev tshuaj xyuas cov tshuaj, thiab kev tshawb nrhiav tshuaj, thiab cov chaw muag tshuaj papaclokinate .
Lub Hom Phiaj Siv Tau Thiab Mechanism ntawm Kev Tshawb Fawb
Fitc sau cov me me molecule inibitors tuaj yeem siv los siv cov tshuaj me me (xws li Gefitinib) los ntawm cov khoom siv hauv cov yeeb yaj kiab (K/}} kuj tau siv los kawm cov gene reepencing efficiency, muab ib lub hauv paus rau RNA cuam tshuam kev kho mob.

Kev Tshuaj Xyuas Kev Siv Tshuaj
FITC sau npe nanoparticles (xws li Liposomes thiab Polymer Oprepeles) tuaj yeem soj ntsuam kev faib tawm thiab tso cov kev coj tus cwj pwm hauv Vivo hauv Real-Time.

 

Cov kev sim tau pom tias kev sib txuam ntawm Fitc sau npe Pegylated li ntsev yog 2}

Tshuaj Hoobkas thiab Kev Kawm Txog Kev Kawm Txuj Ci
FITC daim ntawv sau cov tshuaj Molecules tuaj yeem raug txheeb xyuas rau lawv cov kev nqus, xws li vancioys yog linearally ua kom zoo rau cov tshuaj concentration, muab ib lub hauv paus rau kev ua yeeb tshuaj noj tshuaj . Nyob rau hauv, Fitc daim ntawv lo qhia CyTotoxicity tseem ceeb tuaj yeem ntsuas kev ntsuas kev nyab xeeb thiab coj cov kev ntsuas kev nyab xeeb {2}

Cov ntaub ntawv tshawb fawb: los ntawm sensors rau fluorescent probes

 

Cov yam ntxwv fluorescence ntawm featc ua rau nws tsis sib haum xeeb, suav nrog kev txhim kho ph sensors, thiab cov hlau ion acces {}}
PHAU F fluorescence Sensor
Lub fluorescence siv ntawmfluoresin isothiocyanate is significantly affected by the pH value of the solution. In weakly acidic environments (pH 5.0-6.5), protonation of FITC groups leads to increased fluorescence intensity; Under alkaline conditions (pH>8 {. 0), fluorescence yog siv los saib cov nqaij me me, muab txoj kev tshiab rau cov qog cov qog.

 

Fluorescent fluorescent sojntsuam
Fluorescence lub neej hloov nrog kub {{}}}}}}} 8 zaug siab dua li chav kub (25 degree). Cov xeeb ceem no ua rau nws yog qhov cuab yeej zoo tshaj plaws rau kev kawm theem hloov theem hloov thiab cell canopreservation.
Kev sojntsuam hlau hlau hlau
Fitc tseem ceeb txo cov kev siv roj fluorescence thaum ua ke nrog kev ntsuas cov hlau xws li, haum rau ib puag ncig cov dej, uas 100 npaug rau cov khoom siv atomic nqus vectroscopy.

Ua liaj / teb thiab tsiaj nyeg: Los ntawm cov pathogen nrhiav kom tau rau ntau yam kev txhim kho

 

Daim ntawv thov ntawm Fitc hauv kev ua liaj ua teb tau nce ntxiv, suav nrog cov kab mob nroj tsuag, suav nrog kev kuaj mob tsiaj .
Nrhiav kom ceev ceev ntawm cov nroj tsuag
FITC daim ntawv lo tshuaj tiv thaiv kab mob tuaj yeem siv los kuaj cov luam yeeb mosaic (TMV), thiab lwm yam khoom siv) Ntxiv rau, Fitc sau nucleic acid probar probes kuj tseem siv tau los kuaj cov noob caj ces hloov kho hauv cov noob caj noob ces.

Kev kuaj mob ntawm cov tsiaj kab mob
FITC daim ntawv lo rau cov tshuaj tiv thaiv tuaj yeem siv los kuaj swine tus kab mob swooter (CSFV), thiab lwm yam virian .

 

Nyob rau hauv kev kuaj mob ntawm swine ua npaws, Fitc sau cov kab mob ntawm cov tsiaj txhu hauv cov tsiaj hauv cov ntshav thiab tshuaj tiv thaiv kab mob tsis zoo los ntawm cov kab mob fluorcetry thiab soj ntsuam lub cev tiv thaiv kab mob flumune.
Muaj ntau yam cim purity
FITC sau daim ntawv DNA tuaj yeem txheeb xyuas qhov tseeb ntawm cov qoob loo ntawm cov qoob loo ntawm 99}}} {{} ntxiv rau hauv ntxiv, FITC sau npe SSR molecular cov cim kuj tseem siv tau rau kev txheeb xyuas cov tsiaj txhu caj ces thiab cov nqaij qaib cov tsiaj txhu, muab cov khoom siv science rau kev yug me nyuam.

Synthesis ntawm FITC: {4- nitrophthhalic anhydride anhyrride (saib C8h3No5, [{}}) Anhydride, hydrolyzes nrog saturated sodium hydrofluorescein yog tau {{{{, thiab txo rau 4 '- amino fluoresCesin . sov tshaj plaws yog acylated thiab tshem tawm kom tau txaisfluoresin isothiocyanate.

Fitc ntshiab cov khoom yog daj los yog txiv kab ntxwv crystalline hmoov, uas yog cov molecular hnyav yog 390 ~ 495 nm, thiab Qhov siab tshaj plaws emissenth yog 520 ~ 530 nm, qhia qhov chaw zoo nkauj ntsuab, thiab yog qhov chaw zoo tshaj plaws hauv cov yeeb yaj kiab daj

Cim npe nrov: Fluoresin Isothiocyanate Ua 27072-45-3, cov chaw muag khoom, chaw tsim khoom, chaw tsim khoom, yuav khoom, nqe, loj, rau muag