Shaanxi Bloom Tech Co., Ltd. yog ib qho ntawm cov neeg tsim khoom thiab cov khoom lag luam f f fuv ua cas 1461-15-0 hauv Suav teb. Txais tos rau kev lag luam wholesale tej ntshav siab zoo fluorexon cas 1461-15-0 rau muag ntawm no los ntawm peb lub hoobkas. Cov kev pabcuam zoo thiab tus nqi tsim nyog muaj.
Fluorexonyog qhov sib txuas ua ke nyob rau hauv cov teb ntawm cov kev siv roj ntsha thiab biology, feem ntau tshwm sim ua cov hmoov daj daj lossis txiv kab ntxwv liab (nws cov ntsev ntsev). Nws muaj cov solubility zoo nyob hauv Ethanol thiab Alkali, tab sis yog soluble me ntsis hauv dej. Thiab nws cov ntsev ntsev yog soluble hauv dej thiab ua pov thawj daj thiab ntsuab fluorescence cov yam ntxwv. Raws li cov fluorescent ntsuas, nws muaj qhov tshwj xeeb fluorescence cov khoom. Tom qab tau yaj nyob rau hauv dej, nws sodium ntsev ua yeeb yam daj thiab ntsuab fluorescence khoom, uas ua rau nws muaj cov ntawv thov tseem ceeb hauv cov liaj teb xws li biology thiab tshuaj. Piv txwv li, hauv cov kev sim cell uas siv tau los ua cytoplasmic zas xim kom soj ntsuam cov kev faib tawm thiab hloov chaw ntawm incas ions. Raws li cov kev sib txuas lus ntau ntau, nws muaj kev tshawb fawb txog tsiaj ntawv xws li Biology thiab kev tshawb fawb kho mob, tshuaj xyuas tshuaj lom neeg thiab kev lag luam. Nws tshwj xeeb fluorescence cov khoom, qis cytotoxicity, thiab zoo chelating muaj peev xwm ua calcein ib qho kev tshawb fawb thiab kev tshawb fawb.
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Tshuaj Cov Qauv |
C30H26N2O13 |
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Muaj pes tsawg leej |
622 |
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Molecular hnyav |
623 |
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m/z |
622 (100.0%), 623 (32.4%), 624 (2.7%), 624 (2.7%), 624 (2.4%) |
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Kev Ntsuam Xyuas |
C, 57.88; H, 4.21; N, 4.50; O, 33.41 |
Fluorexon, raws li kev sib txuas ntau ntau, muaj ntau cov ntawv thov hauv kev tshawb fawb thiab chaw lag luam.
1. Biology thiab Tshawb Nrhiav Kho Mob
(1) Xov tooj ntawm tes thiab sim:
Calcein yog ib qho tseem ceeb fluorescent zas xim rau kev yees duab, uas tuaj yeem tau txais los ntawm cov molecular cloning thiab tshuaj synthesis txoj kev. Nws yog dav siv hauv cell tsom xam, cov protein hauv zos, thiab kev tshawb fawb ntawm Intracellular teeb liab kev hloov chaw. Calcein tuaj yeem ua tau zoo thiab ntxim nyiam cov kev hloov pauv hauv calcium ion concentration, muab ib qho cuab yeej muaj zog rau kev tshawb fawb cellular calcium, thiab tshuaj ntsuam xyuas tshuaj.
(2) Kev nquag Cell Nrhiav tau:
Calcein feem ntau siv los ua kev sojntsuam fluorescent los xyuas cov kev loj hlob ntawm cov hlwb muaj sia nyob. Los ntawm nws cov bytotoxicity, Calceoti tuaj yeem dawb diffuse los ntawm cytopalescence, thiab tom qab ntawd saib cov fluorescence tsom rau ntsuas cov haujlwm thiab kev ua haujlwm ntawm tes.
(3) Kev Ntsuas Tshuaj Yeeb Tshuaj thiab Kev Ntsuas Xyuas Kev Ntsuas:
Calcein muaj feem xyuam rau kev txiav txim siab ntawm ntau cov tshuaj, suav nrog CyTotoxicity, oxidative muaj nuj nqi, thiab neurotoxicity. Nyob rau hauv cov txheej txheem ntawm kev tshuaj xyuas tshuaj thiab kev soj ntsuam toxicity, Calcein tuaj yeem siv los tshawb xyuas qhov kev cuam tshuam ntawm cov tshuaj ntawm kev nyab xeeb thiab tshuaj noj cov tshuaj.
2. Kev soj ntsuam tshuaj lom neeg thiab nrhiav kom tau
(1) qhov taw qhia nyuaj:
Calcein, raws li ib qho qhia tau nyuaj, muaj ntau cov kev siv hauv kev tshuaj ntsuam xyuas tshuaj lom neeg. Nws yog tsuas yog siv rau kev ua si ntawm lub taub ntswg ntawm calcium. Los ntawm nws cov cim fluorescence cov khoom, cov xim hloov pauv thaum lub sijhawm ua haujlwm tuaj yeem ua tiav kev ntsuas ntawm calcium ion confulation.
(2) Fluorescent taw qhia:
Calcein ntsuab kuj tseem siv tau ua qhov ntsuas fluorator los ntes cov hlau ions xws li kev kuaj fluorium, yog li ua tiav kev kuaj pom thiab kom ntsuas ntawm cov hlau ions.
(3) Cov tshuaj fluorescence fluorescence fluorescence:
Nyob rau hauv cov fluorescence kom pom ntawm qhov ntsuas kub tsis tu ncua, Calcein kuj tseem ua lub luag haujlwm tseem ceeb. Thaum lub amplification cov neeg tiv thaiv pyrophosphaate ions, manganese ions ua ke nrog pyganosphat ions los ua manganosphate, ua rau tiam neeg fluorescence. Los ntawm kev soj ntsuam cov kev hloov pauv hauv fluorescence tsom, cov txheej txheem thiab cov txiaj ntsig ntawm kev tiv thaiv zoo tuaj yeem taug qab hauv tiag tiag- lub sij hawm.
3. Muaj cov ntawv thov
(1) Qhov taw qhia thiab zas:
Calcein tuaj yeem siv ua qhov ntsuas thiab zas xim hauv cov lag luam. Piv txwv li, hauv kev tshuaj ntsuam ntawm Tshuaj phosphote ore, calcium daj ntsuab yuav siv tau los ua calcium ntsuab qhia, qhia cov ntsiab lus calcium daj ntsuab, qhia txog cov ntsiab lus calcium daj hauv phosphate ore los ntawm nws cov xim hloov. Tsis tas li ntawd, calcium daj ntsuab kuj tseem siv tau rau Dyeing thiab xim hauv kev lag luam xws li cov ntawv thiab tawv.
(2) Kev Saib Xyuas Ib puag ncig:
Calcium Daj ntsuab xim xim kuj muaj qee qhov kev thov tus nqi hauv kev saib xyuas ib puag ncig. Piv txwv li, hauv kev soj ntsuam zoo, Calcein tuaj yeem siv los kuaj cov calcium rau hauv dej, yog li ntsuas lub hardness thiab℃ntawm kev ua qias tuaj. Tsis tas li ntawd, Calcein kuj tseem siv tau los tshawb xyuas cov hlau hnyav thiab lwm cov kuab paug hauv dej qias neeg.
Fluorexon, nrog tshuaj chemical c ₂₆ n ₂ n ₂ n ₂ o α α, yog lub ntsej muag daj huab α, yog cov ntsev ua kom zoo nkauj txiv kab ntxwv txiv kab ntxwv liab cov muaju. Raws li "Chameleon" hauv kev ua haujlwm fluorescent probes, Calcein tau pom meej txog tus nqi tshuaj rau hauv cov khoom siv fluorescence, kev xaiv fluorescence, kev xaiv siab thiab tsawg toxicity. Cov nram qab no elaborates ntawm nws lub hom phiaj los ntawm ntau qhov ntev:
1. Cov ntsiab lus tseem ceeb ntawm lub sijhawm
Calcein yog qhov qhia tau zoo tshaj plaws rau cov hlau tsis xws li cov kab hlau xws li calcium, strontium, tooj liab, thiab manganese. Ntau cov pawg carboxyl ({- Coon) hauv nws cov qauv molecular, ua rau cov qauv molecular, qhia tau tias qhov kawg, qhia txog qhov kawg ntawm lub kaum kev. Piv txwv li, hauv cov calcium ion, thaum Calcein chelates nrog Edta (EthylenedoMinetTiver sason), thiab qhov rhiab ntawm cov kev txiav txim siab endpection ncav cuag 0.1 μ mol / l. Hauv xyoo 1970, cov thev naus laus zis no tau qhia txog kev cuam tshuam hauv av (xws li chrome dub t) ion nag lossis pib ua kom tau qhov tseeb.
2. Fluorescence Nrhiav Kev Soj Ntsuam
Calcein emits Lub teeb daj ntsuab (λ em =515}} ion ion concium concium concortration hauv kev kuaj txheeb. Nws muaj kev xaiv zoo dua li fluorescein, qis dua kev sib cav sib haum nrog cov dej khov nab kuab thiab kom haum rau cov calcium ion, thiab cov dej kom haum rau cov tshuab ntshav xws li cov ntshav thiab cov kua ntshav. Piv txwv li, hauv kev kawm txog calcium taw qhia hauv myocardial liab qab calcium ion kev hloov pauv hauv lub sijhawm, pom lub molecular txheej txheem ntawm arrhythmia.
3. Ntau Metallic Kev Tshawb Xyuas
Ntxiv rau cov tshuaj calcium, calcein kuj tseem siv tau rau kev kuaj pom ntawm cov tog, liab, mangal, cickel, molybdenum, thiab chromium ions. Xaiv kev txiav txim siab ntawm ions tuaj yeem ua tiav los ntawm kev kho cov ph ntawm cov kev daws teeb meem lossis ntxiv cov ntawv pov thawj npog ntsej muag xws li Edta. Piv txwv li, hauv kev saib xyuas ib puag ncig, Calcein ua ke nrog ion chromatography tuaj yeem saib ib lub tshuab hluav taws xob ntau hauv cov dej ntawm PPB.
Kev Tshawb Fawb Biomedical: Tus 'Fluorescent Key' rau Cell Kev Tshawb Fawb thiab Kab Mob Kev
1. Staining thiab ua haujlwm tsom xam ntawm cov ciaj sia
CalCEIN AM: Raws li Membrane permotised fluorescent esterase actorase, calceSin Vim tias tsis muaj cov kev ua ub no nyob rau hauv cov hlwb tuag, cov kev sojntsuam no tsuas yog cov cim ntawm kev kuaj mob hauv xov tooj, luv} lub sij hawm labelling, thiab nus cytometry kev tsom xam. Piv txwv li, nyob rau hauv kev kawm cov qog cell cov tshuaj tiv thaiv, kev sib xyaw ua ke ntawm Caloin, thiab cov khoom siv apoptotic, thiab necrotic hlwb nrog cov rhiab ntawm ntau tshaj 95%.
Multicolor fluorescent derivatives: To address the limitation of single color, AAT Bioquest has developed blue (Calcein Blue AM, λ em=441nm) and orange (Calcein Orange) ™ Diacetate,λem=545nm), Calcein Red ™ AM, λ em=576nm) and Calcein Deep Red ™ AM, λ {=663}}}}}}}}}}}}}}} gultion} daim xim sau npe thiab tsom xam cov hlwb. Piv txwv li, hauv kev tiv thaiv kab cell kev coj noj coj ua, kev sib xyaw ua ke ntawm Calcein Blue tuaj yeem taug qab cov cell tsiv teb chaws thiab kev sib tham hauv tiag {6 sijhawm.
2. Cov pob txha metabolism tshawb fawb
Calcein yog tus tswj hwm "fluorescent" rau kev kawm pob txha metabolism. Nws cov calcium nyob fluorescence cov khoom ua kom muaj peev xwm ua rau nws yuav tsum tau ua piv txwv li micro computed), nws tuaj yeem tshawb xyuas cov kab mob pob zeb ua haujlwm thiab kev kawm mineralization. Piv txwv li, hauv kev kawm cov kab mob epeoporosis, cov qauv siv ob daim ntawv qhia kev txhaj tshuaj ntawm cov pob txha sib hloov) tuaj yeem muab ib qho kev hloov pauv ntawm cov pob txha sib txawv.
3. Kev tshuaj xyuas cov tshuaj
Calcein tuaj yeem raug siv ua qauv tshuaj los ntsuas lub cellular uptake thiab tso tawm kev ua tau zoo ntawm cov zaub mov thiab cov ligoosomes thiab liposomes. Piv txwv li, hauv kev tshawb fawb ntawm ovi {{1} akopartics ntawm cov nanoparticles ntim tau tom qab tau coj los ntawm kev ua kom muaj kev cuam tshuam ntawm cov cab kuj.
Cov ntaub ntawv kev tshawb fawb: Fluorescent additives rau cov ntsiab muag iav thiab biomaterials
1. Kev ntsuam xyuas ntawm cov tawv muag muag muag muag
Calcein tuaj yeem siv rau cov pa oxygen permeability thiab NTO NTUJ Morphology Nrhiav tau ntawm cov iav muag muag muag muag. Nws cov yam ntxwv fluorescence tuaj yeem qhia cov qauv microporous thiab noo noo faib rau hauv cov ntaub ntawv lens, tsim kho cov khoom tsim. For example, in the research and development of silicon hydrogel lenses, the calcein staining method can quantitatively analyze the oxygen permeability coefficient of the lenses to ensure wearing comfort.
2. Tracer ntawm biodegradable cov ntaub ntawv
Calcein tuaj yeem siv tau ua ib qho tracer kom daim ntawv lo qhia cov txheej txheem depradation xws li polylactic acid thiab polycaprolactone. Los ntawm fuzorescence microscopy soj ntsuam, qhov ntsuas cov nqi thiab cov khoom lag luam hauv Vivo tuaj yeem taug qab hauv kev tsim cov ntaub so ntswg ua rau cov kiav txhab ua vaj tsim.
1. Cov roj rho tawm
Calcium ntsuab xim, raws li drilling kua quav ntxiv, tuaj yeem txhim kho cov roj av nplua nuj los ntawm cov teebmeem thickening thiab ruaj khov. Nws cov tshuaj feem ntau yog 0.05%-} 0.2%, uas tuaj yeem tiv taus ntau qhov chaw kub thiab txo qis kev puas tsuaj. Piv txwv li, hauv kev sib sib zog nqus dej, Calcein tuaj yeem txo cov kev lim dej thiab tiv thaiv kev ua haujlwm zoo.
2. Textile Printing thiab Dyeing
Calcein, raws li cov tshuaj ntxuav hniav tshuaj zoo nkauj, tuaj yeem siv rau qhov ua kom lub ntsej muag ntuj xws li cov paj rwb thiab hemp. Nws cov fluorescence daj tau tuaj yeem txhim kho qhov dawb huv thiab kev sib tw ntawm cov ntaub, thiab nws muaj cov tshuaj ntxuav tau zoo heev. Piv txwv li, hauv kev tsim cov siab -} Cov ntawv dai ntawm lub txaj, cov qauv ntawm cov khoom lag luam los ntawm 20% -30%, ntsib cov qauv xa tawm.
Cov teb tawm: Cov khoom lag luam tshiab ntawm 3D luam ntawv thiab kev txawj ntse ntim khoom
1. 3 d luam tawm ntawm biomaterials
Calcium ntsuab xim thiab nanocellulose composite tuaj yeem siv los npaj biodegradable 3D luam tawm scaffolds. Nws cov yam ntxwv fluorescence txhawb tiag-} Kev saib xyuas cov txheej txheem luam ntawv, kom paub tseeb cov qauv txheej txheem. Piv txwv li, hauv cov ntaub so ntswg ua lag luam, calcium ntsuab xim sau cov qauv scaffolds tuaj yeem coj cov kab ke ntawm kev loj hlob thiab txhawb lub hauv paug.
2. Cov ntaub ntawv khaws cia ntse
Calcium xim ntsuab raws cov ntim ntse tuaj yeem saib xyuas cov khoom noj tshiab ntawm cov zaub mov. Los ntawm kev siv cov xim hloov los qhia txog cov nqaij lwj, cov rhiab tau mus txog PPM qib. Piv txwv li, hauv cov tshuaj xa khoom txias, cov fluorescence quenching ntawm calcium Daj yeeb yaj kiab ntsuab (xws li Putrestrespine) tuaj yeem intruitally xav txog cov zaub mov.
Lub sim synthesis txoj kev ntawmFluorexonmas tsuas yog ob txoj hauv kev tseem ceeb: molecular cloning txoj kev thiab tshuaj lom neeg synthesis txoj kev. Cov hauv qab no yuav piav ntau ntxiv rau ob txoj kev thiab lawv cov tshuaj sib txig sib luag.
1. Molecular cloning txoj kev
Molecular cloning yog ib txoj kev npaj calcein los ntawm kev siv thev naus laus zis ntawm caj ces. Cov tswv yim yooj yim ntawm hom no yog kom rho tawm cov noob ua ntu zus los ntawm cov tshuaj lom neeg fluorescent nyob rau hauv cov lus teb jellya victoria (ntsuab
Theem Taw Qhia
1. Tau txais cov noob le caag:
Firstly, rho tawm cov noob ua ntu zus ntawm fluorescent protein los ntawm ntsuab jellya victoria.
2. Gene Cloning:
Siv cov txheej txheem genetic engetic engineering, cov noob le ua kab ke ntawm fluorescent proteins yog cloned rau hauv vector tsim nyog, xws li plasmid.
3. Kev nthuav qhia system tsim:
Plasmids muaj fluorescent protein cov gene ua ntu zus tau los qhia rau hauv cov tshuab piav qhia xws li Escherichia Coli rau kev cog qoob loo thiab kev piav qhia.
4. Extraction thiab Purification ntawm Calcein:
Los ntawm cov txheej txheem biochemical, Calcein tau muab rho tawm thiab ua kom huv los ntawm cov kab ke hais tawm.
2. Tshuaj Kho Mob Hluav Taws Xob
Cov tshuaj lom hluav taws xob hluavtaws hluavtaws yog los ua ke ua ke cov tshuaj fluorescent ntawm cov tshuaj fluorescent ntawm Codeech los ntawm cov tshuaj lom neeg tshuaj lom neeg. Txoj kev no xav siv cov ntaub ntawv raw tshuaj lom thiab cov tshuaj lom neeg ntawm cov tshuaj lom neeg los txhawb calcein.
Theem Taw Qhia
1. Cov Khoom Siv Khoom Siv:
Npaj cov ntaub ntawv tshuaj raw cov tshuaj nyoos, xws li fluorescein, sodium hydroxide, iminodiacetic acid, formaldehyde, thiab lwm yam.
2. Cov tshuaj tiv thaiv:
Cais fluorescein hauv ethanol, ntxiv cov ntaub ntawv raw xws li sorium hydroxide, iMinodiacete acid, formathyde, thiab lwm yam khoom siv hluav taws xob hauv qab thiab cov xwm txheej.
3. Kev huv thiab Crystallization:
Los ntawm cov kauj ruam xws li dej huv huv, kev tsis huv tau raug tshem tawm kom tau txais cov calcium daj daj ntsuab. Yog tias cov ntsev ntsev ntsev ntawm Calcein yog xav tau, ntxiv cov khoom siv ntsev ntxiv tuaj yeem nqa tawm.
Cov txheej txheem hluavtaws hluavtaws yuav suav nrog cov tshuaj tsw qab li hauv qab no:
(1) c20h12O 5+ C2h6o → → FluoresCein ESANOL daws
(2) fluorescein fluoresin ethanol daws + Naoh + c4h7NO 4+ → Ch2o → Kev Tawm Tsam Nruab Nrab
(3) Cov tshuaj tiv thaiv Intermediates → c30h26n2o {{}}- cov khoom (xws li H2O)
Thov nco ntsoov tias txoj kab sib npaug saum toj no tsuas yog ua piv txwv, thiab txheej txheem tiag tiag tej zaum yuav nyuaj thiab tuaj yeem koom nrog ntau theem nrab thiab cov khoom lag luam.
Chav ntsuas hluav taws xob saptthesis ntawm calcein tsuas yog suav nrog molecular cloning thiab tshuaj schtesis. Molecular cloning siv cov txheej txheem caj ces archeticyering cov roj ntsha ua ntu zus los ntawm cov cuab yeej muaj sia, thiab cov ntaub ntawv clones thiab clones thiab clones thiab qhia lawv nyob hauv ib qho kev qhia; Cov Tshuaj Siv Tshuaj Tynthesis txoj cai synthesizesfluorexonlos ntawm cov ntaub ntawv zam tshuaj lom neeg thiab tshuaj lom neeg. Ob txoj kev no txhua tus muaj lawv tus kheej cov yam ntxwv thiab haum rau kev tshawb fawb kev xav tau thiab kev siv cov xwm txheej. Hauv kev siv cov tswv yim, cov qauv hluav taws xob tsim nyog tuaj yeem raug xaiv raws cov kev tshawb fawb tshwj xeeb thiab kev sim mob.
Cim npe nrov: Fluorxon Cas 1461-15-0, Tus neeg muab khoom, chaw tsim khoom, Hoobkas, lag luam wholesale, yuav, nqe, loj, loj, rau muag