Puerarintseem hu ua 8-( -D-Glucopyranosyl-7-hydroxy-3-(4-hydroxyphenyl)-4H-1-benzopyran-4- ib, yog ib tsob nroj isoflavonoid derivative muab los ntawm cov hauv paus hniav qhuav ntawm Pueraria lobata (Willd.) Ohwi, ib tsob nroj leguminous Nws muaj ntau yam tshuaj Cov kev ua ub no, suav nrog txo cov ntshav siab, ntshav lipids, thiab ntshav qabzib, tshem tawm cov dawb radicals, thiab nthuav tawm cov khoom siv tshuaj tiv thaiv kab mob antioxidant.
Peb muab Puerarin Powder CAS 3681-99-0, thov xa mus rau lub vev xaib hauv qab no kom paub meej cov lus qhia tshwj xeeb thiab cov ntaub ntawv khoom.
Chemical Properties thiab Extraction
Puerarin muaj qhov hnyav molecular ntawm 416.37 thiab muaj nyob rau hauv ib rab koob dawb zoo li siv lead ua nyob rau hauv methanol-acetic acid, nrog lub melting point ntawm 187 degree (decomposition). Cov qauv tshuaj ntawm puerarin muaj cov pob txha flavonoid hloov nrog -D-glucopyranosyl moiety.
Puerarin yog muab los ntawm cov hauv paus hniav qhuav ntawm Pueraria lobata, cov nroj tsuag ib txwm siv hauv Suav tshuaj. Txhawm rau cais cov puerarin, cov kws tshawb fawb siv kev sib xyaw ua ke ntawm cov kuab tshuaj thiab cov txheej txheem ua kom huv huv kom tshem tawm cov tshuaj no kom zoo los ntawm cov khoom cog. Feem ntau, cov kuab tshuaj xws li methanol, ethanol, los yog ua ke ntawm cov no nrog acetic acid yog siv los yaj thiab rho tawm puerarin los ntawm cov hauv paus hniav qhuav. Tom qab kev rho tawm, cov kua txiv hmab txiv ntoo qhuav tau dhau los ua cov txheej txheem purification kom tshem tawm cov impurities thiab xyuas kom meej qhov purity ntawm cov khoom kawg. Cov txheej txheem purification no yuav suav nrog crystallization, chromatography, los yog lwm txoj hauv kev kom cais cov puerarin los ntawm lwm cov khoom hauv cov extract.
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Cov teebmeem plawv
Puerarin nthuav tawm ntau qhov txiaj ntsig zoo ntawm cov hlab plawv, ua rau nws muaj txiaj ntsig zoo hauv kev kho mob plawv.
Kev tswj hwm puerarin rau cov nas ntawm kev txhaj tshuaj intraperitoneal lossis subcutaneous tau pom tias tiv thaiv tus mob myocardial ischemia vim los ntawm pituitrin. Hauv cov dev uas tau txhaj tshuaj loog, intracoronary infusion ntawm puerarin ua rau kom cov ntshav ntws mus rau hauv cov hlab ntsha thiab txo cov vascular tsis kam. Intravenous tswj kuj ua rau kom cov ntshav txaus nyob rau hauv cov hlab ntsha carotid thiab femoral, txawm tias tsawg dua hauv cov hlab ntsha. Cov nyhuv ntawm puerarin ntawm cov hlab ntsha tseem nyob mus ntxiv txawm tias tom qab kev tswj hwm reserpine, qhia txog kev so ncaj qha rau cov leeg nqaij leeg.
Puerarin txo qis cov lus teb los yog hypotensive rau norepinephrine thiab acetylcholine hauv cov tsiaj rog. Kev txhaj tshuaj ntawm puerarin hauv cov neeg mob ntshav siab ua rau txo qis ntshav siab, lub plawv dhia, thiab qib plasma catecholamine, qhia tias muaj peev xwm txo qis hauv kev ua haujlwm ntawm lub paj hlwb. Tsis tas li ntawd, puerarin tawm tsam cov teebmeem excitatory ntawm isoproterenol ntawm kev sib cais lossis hauv vivo lub siab thiab txo qis lub plawv dhia thiab ntshav siab.
Puerarin ntawm ib koob ntawm 100 mg / kg tuaj yeem tiv thaiv chloroform-adrenaline-induced arrhythmia hauv luav. Hauv cov npua guinea raug tshuaj lom nrog ouabain, puerarin ua rau muaj kev nce siab hauv ventricular ectopic (VE) thiab ventricular tachycardia (VT) qhov pib tab sis muaj qhov cuam tshuam tsawg dua ntawm ventricular fibrillation (VF) qhov pib. Txawm li cas los xij, puerarin tsis zoo dua li propranolol thiab pronethalol hauv antagonizing arrhythmias induced los ntawm aconitine.
Intravenous puerarin kev tswj hwm txo qis qhov kev sim mob myocardial infarction hauv cov dev, tau lees paub los ntawm epicardial electrocardiography, enzymes, myocardial radionuclide scanning, thiab N-BT pathological staining. Hauv cov dev uas siv tshuaj loog, puerarin tsis cuam tshuam rau myocardial contractility tab sis ua rau cov ntshav txaus txaus thaum lub sij hawm hauv cheeb tsam myocardial ischemia (thiab tseem ua rau cov ntshav ntws hauv cov cheeb tsam uas tsis yog ischemic) thiab txo qis hemodynamic tsis cuam tshuam nrog myocardial oxygen noj.
Tshuaj pleev xim rau ntawm 0.5% puerarin hauv cov nas antagonizes adrenaline-vim cov hlab ntsha constriction, txo qis tshaj tawm, thiab txo cov ntshav ntws. Kev tswj hwm 1% puerarin tom qab adrenaline-induced microcirculatory cuam tshuam ua rau muaj txiaj ntsig zoo sib xws. Puerarin intravenous ntawm 52 mg / kg ua raws li adrenaline infusion attenuates adrenaline-induced microarterial constriction, txo qis tshaj tawm, thiab txo cov ntshav txaus, outperforming papaverine. Puerarin kuj tseem txhim kho qhov qub hamster lub hlwb microcirculation thiab microcirculatory cuam tshuam los ntawm cov tshuaj norepinephrine los ntawm kev ua kom microvascular amplitude thiab hauv zos microvascular ntshav txaus.
Antioxidant thiab Anti-inflammatory teebmeem
Puerarin muaj cov tshuaj tiv thaiv kab mob antioxidant thiab tiv thaiv kab mob, uas ua rau nws muaj kev tiv thaiv tiv thaiv ntau yam kab mob oxidative kev nyuaj siab.
Dawb Radical Scavenging
Puerarin ua raws li cov dawb radical scavenger, tiv thaiv cov hlwb los ntawm kev puas tsuaj oxidative. Cov kev tshawb fawb tau pom tias puerarin tuaj yeem tshem tawm cov pa oxygen reactive (ROS) thiab txo cov kev ntxhov siab oxidative, yog li tiv thaiv cellular qauv thiab kev ua haujlwm.
Tiv thaiv Cadmium-induced puas
Kev tshawb fawb tau pom tias puerarin nthuav tawm cov teebmeem tiv thaiv cadmium (Cd)-vim kev puas tsuaj hauv pakchoi (ib hom zaub qhwv hauv Suav). Kev kho mob nrog puerarin thiab Cd txo qis oxidative kev puas tsuaj, txo hydrogen peroxide (H2O2) thiab malondialdehyde (MDA) cov ntsiab lus, thiab nce chlorophyll, vitamin C (Vc), thiab cov ntsiab lus ntawm cov protein ntau, nrog rau cov kev ua haujlwm antioxidant. Cov kev tshawb pom no qhia tias ib koob tshuaj uas tsim nyog ntawm puerarin tuaj yeem txo qis Cd-induced inhibition ntawm kev loj hlob saum toj kawg nkaus hauv pakchoi thiab txo Cd uptake.
Cov nyhuv hypoglycemic
Puerarin nthuav tawm kev ua haujlwm hypoglycemic, ua rau nws muaj peev xwm kho tus neeg mob rau ntshav qab zib mellitus.
Kev tswj hwm puerarin rau alloxan-induced hyperglycemic nas ntawm koob tshuaj 250 thiab 500 mg / kg / hnub rau 4-5 hnub sib law liag txo cov ntshav qabzib ntau. Cov nyhuv hypoglycemic ntawm 500 mg / kg puerarin tau tshaj tawm, nrog 250 mg / kg yog ze rau qhov tsawg kawg nkaus koob tshuaj. Tsis tas li ntawd, kev sib xyaw ua ke ntawm puerarin thiab aspirin muaj qhov sib piv cov txiaj ntsig hypoglycemic rau cov koob tshuaj siab siab puerarin ib leeg, nrog rau cov nyhuv hypoglycemic kav ntev dua 24 teev thiab txhim kho cov piam thaj hauv cov nas alloxan-induced hyperglycemic.
Antiplatelet Aggregation
Puerarin inhibits platelet aggregation, uas yog qhov tseem ceeb hauv kev tiv thaiv kab mob plawv xws li thrombosis thiab myocardial infarction.
Inhibition ntawm Platelet Aggregation
Cov kev tshawb fawb hauv vitro tau pom tias puerarin inhibits ADP-induced platelet aggregation nyob rau hauv nas thiab cov aggregation ntawm luav, yaj, thiab tib neeg platelets induced los ntawm 5-hydroxytryptamine (5-HT) thiab ADP. Tsis tas li ntawd, puerarin cuam tshuam qhov kev tso tawm ntawm ³H-5-HT los ntawm platelets ntxias los ntawm thrombin ntawm qee qhov concentration.
Toxicity thiab Adverse Reactions
Qhov koob tshuaj nruab nrab ntawm qhov tuag taus (LD50) ntawm puerarin hauv cov nas yog 738 mg / kg thaum noj cov tshuaj intravenously, qhia tias nws muaj toxicity tsawg. Txawm li cas los xij, cov kev phiv xws li kiv taub hau, mob taub hau, thiab xeev siab tau raug tshaj tawm nyob rau hauv qee kis, tshwj xeeb tshaj yog siv ntau npaum li cas los yog siv ntev. Yog li ntawd, nws yog ib qho tseem ceeb los saib xyuas cov neeg mob kom zoo thaum lub sij hawm kho puerarin thiab kho cov tshuaj raws li kom txo tau qhov tsis zoo.
Xaus
Puerarin yog ib qho kev sib xyaw ua ke nrog ntau yam kev ua haujlwm tshuaj. Nws cov teebmeem ntawm cov hlab plawv, nrog rau cov hlab ntsha thiab lub hlwb vasodilation, antihypertensive zog, anti-arrhythmic kev ua, thiab muaj txiaj ntsig zoo ntawm myocardial infarction thiab ischemia, ua rau nws muaj txiaj ntsig zoo rau cov kab mob plawv. Tsis tas li ntawd, puerarin's antioxidant thiab tiv thaiv kev ua ub no tiv thaiv cov hlwb los ntawm kev puas tsuaj oxidative thiab o, thaum nws cov teebmeem hypoglycemic thiab antiplatelet aggregation ntxiv nthuav dav nws txoj kev kho mob. Txawm hais tias nws cov tshuaj toxicity tsawg, kev soj ntsuam ze ntawm cov neeg mob thaum lub sij hawm kho puerarin yog qhov tseem ceeb kom txo qis cov kev tsis zoo. Kev tshawb fawb yav tom ntej yuav tsum tsom mus rau kev nthuav tawm cov txheej txheem hauv qab ntawm puerarin cov tshuaj ua haujlwm thiab tshawb nrhiav nws lub peev xwm hauv kev kho mob ntawm lwm yam kab mob.