cjc 1295 Peptide ua, CAS 863288-34-0. Feem ntau yog cov hmoov dawb los sis zoo sib xws, amorphous lossis fluffy, yooj yim rau yaj hauv cov kuab tshuaj polar xws li dej, methanol, ethanol, tab sis nyuaj rau yaj hauv cov kuab tshuaj tsis-polar xws li chloroform, dichloromethane, thiab lwm yam. Ntawm chav tsev kub, CJC1295 muaj kev ruaj ntseg siab. thiab tuaj yeem khaws cia thiab thauj mus rau hauv qhuav, txias thiab tsaus ntuj, thiab lub sijhawm siv tau yog feem ntau 2-3 xyoo. CJC -1295 yog ib qho polypeptide GHRH (kev loj hlob hormone tso tawm cov tshuaj hormones), uas tuaj yeem txhawb kev tso tawm cov tshuaj hormones (HGH) secreted los ntawm lub caj pas pituitary, thiab yog li tau siv dav hauv ntau qhov chaw kho mob thiab tsis yog chaw kho mob.
Hais txog CJC 1295, peb muaj 3 lub vev xaib los qhia thiab piav qhia cov khoom kom ntxaws raws li cov khoom xyaw sib txawv, uas yog:
CJC 1295 Nrog DAC : https://www.bloomtechz.com/synthetic-chemical/peptide/cjc-1295-with-dac-cas-863288-34-0.html
CJC 1295 Peptide hmoov: https://www.bloomtechz.com/synthetic-chemical/peptide/cjc-1295-peptide-cas-863288-34-0.html
CJC -1295 Acetate: https://www.bloomtechz.com/synthetic-chemical/peptide/cjc-1295-acetate-cas-863288-34-0.html
1. Acidity thiab alkalinity:
CJC -1295 yog ib qho tsis muaj zog acidic amino acid polypeptide nrog pKa tus nqi ntawm 4.8. Qhov no txhais tau hais tias nyob rau hauv ib puag ncig acidic hauv qab pH 4.8, CJC -1295 muaj nyob rau hauv ib daim ntawv ionized, thaum nyob rau hauv ib tug nruab nrab los yog yooj yim ib puag ncig tshaj pH 4.8, nws muaj nyob rau hauv ib tug non-ionized daim ntawv.
Nws tseem tsim nyog sau cia tias yog CJC-1295 raug rau tej yam kev mob xws li cua sov los yog ultraviolet (UV) hluav taws xob, nws cov molecules tej zaum yuav degraded, yog li yuav tsum tau saib xyuas los xaiv cov khoom tsim nyog thiab siv txoj kev.
2. Oxidation-txo cov khoom:
Cov khoom redox ntawm CJC-1295 feem ntau yog cuam tshuam nrog cov sulfur bonds nyob rau hauv. Sulfur bonds tuaj yeem tsim kev ruaj khov disulfide txuas hniav thiab tuaj yeem txo qis thaum muaj cov neeg ua haujlwm txo kom hloov lawv cov qauv molecular. Piv txwv li, cysteine amino acid nyob rau hauv CJC -1295 raug txo kom cysteine nyob rau hauv lub xub ntiag ntawm daim txhuam cev hlau (FeS) catalyst.
Tsis tas li ntawd, CJC -1295 kuj tuaj yeem oxidized, feem ntau yog siv hydrogen peroxide los yog lwm yam oxidizing agents ua reagents. Txij li CJC -1295 yog cov saw hlau polypeptide uas muaj ntau cov amino acid residues, cov khoom sib xyaw ua ke yuav tshwm sim hauv cov tshuaj tiv thaiv oxidation, uas yuav tsum tau txheeb xyuas thiab tshuaj xyuas los ntawm kev sib cais thiab kev ua kom huv.
3. Cov tshuaj tiv thaiv kev sib koom tes:
CJC-1295 muaj ntau cov electrophilic atoms, xws li nitrogen, oxygen thiab sulfur, uas tuaj yeem tsim kev sib koom tes nrog cov hlau ions. Cov tshuaj tiv thaiv kev sib koom tes ntawm CJC-1295 thiab qee cov hlau ions tau raug tshawb fawb ntau thiab nws tau pom tias cov tshuaj tiv thaiv no muaj peev xwm ua haujlwm lom neeg.
Piv txwv li, CJC -1295 tuaj yeem koom tes nrog zinc ions los ua ib qho chaw ruaj khov. Cov complex no muaj cov txiaj ntsig ntawm kev txhawb nqa cell proliferation thiab kho cov ntaub so ntswg puas, yog li nws yuav tsum tau los ua tus neeg sawv cev kho.
4. Ntxiv cov tshuaj tiv thaiv:
CJC-1295 kuj tseem koom nrog ntau qhov kev tawm tsam ntxiv uas peptide daim ntawv cog lus ntawm CJC-1295 tuaj yeem tawg thiab tsim cov pab pawg ua haujlwm tshiab. Piv txwv li, CJC-1295 tuaj yeem tsim cov amino acids dawb thiab cov khoom sib xyaw ua ke ntawm CJC-1295 los ntawm amide bond hydrolysis, uas tej zaum yuav muaj cov dej num sib txawv.
Tsis tas li ntawd, CJC -1295 kuj tuaj yeem koom nrog cov tshuaj tiv thaiv kev sib raug zoo, cov tshuaj disulfide sib cais cov tshuaj tiv thaiv, thiab lwm yam. Cov tshuaj tiv thaiv no feem ntau siv cov tshuaj txo qis, peroxides, lossis lwm yam tshuaj reagents raws li cov tshuaj tiv thaiv. Txawm li cas los xij, nws yuav tsum raug sau tseg tias cov kev cuam tshuam no tuaj yeem ua rau muaj kev hloov pauv hauv cov qauv molecular ntawm CJC-1295, yog li cuam tshuam rau nws cov kev ua haujlwm lom neeg thiab kev ua tau zoo.
5. Cyclization cov tshuaj tiv thaiv:
CJC -1295 yog cov saw hlau polypeptide uas muaj 29 amino acids, ntau yam muaj cov chaw ua haujlwm. Yog li ntawd, CJC-1295 tuaj yeem koom nrog ntau yam kev cuam tshuam cyclization los tsim cov khoom siv ruaj khov thiab ua haujlwm tau zoo. Piv txwv li, CJC-1295 thiab C6 amino acids yog txuas los ntawm amide bonds los ua ib tug C6-CJC-1295 nplhaib qauv. Daim ntawv cyclized no zoo ib yam li cov qauv ntawm somatostatin-zoo li peptide thiab tej zaum yuav muaj ntau dua los tiv thaiv kev loj hlob hormone tso tawm cov tshuaj hormones (GHRH).
6. Enzyme hydrolysis cov tshuaj tiv thaiv:
CJC-1295 tuaj yeem raug tawm tsam los ntawm cov enzymes xws li protease thiab lyase hauv vivo, ua rau muaj kev hloov pauv hauv cov qauv molecular thiab txo qis hauv kev ua haujlwm lom neeg. Piv txwv li, nyob rau hauv qhov kev txiav txim ntawm trypsin, peptide daim ntawv cog lus hauv CJC -1295 tej zaum yuav hydrolyzed los tsim cov khoom catalytic xws li dipeptide lossis monopeptide, yog li cuam tshuam rau nws cov txiaj ntsig.
Txhawm rau txhim kho bioavailability thiab kev ua tau zoo ntawm CJC-1295, cov kws tshawb fawb tau tsim ntau yam kev ruaj khov thiab cov tshuaj tiv thaiv, xws li PEGylated thiab acetylated CJC-1295. Cov tebchaw no muaj kev ruaj ntseg ntau dua thiab muaj sia nyob ntev dua, thiab xav tias yuav dhau los ua cov tshuaj kho tau zoo dua.
7. Cov qauv molecular:
CJC1295 yog polypeptide molecule muaj li ntawm 29 amino acid residues, nws ib theem zuj zus yog Tyr-D-Ala-Asp-Ala-Ile-Phe-Thr-Gln-Ser-Tyr-Arg-Lys-Val-Leu-Ala-Gln-Leu -Ser-Ala-Arg-Lys-Leu-Leu-Gln-Asp-Ile-Leu-Ser-Arg-NH2 (YD-(a-ADAIF)QSYRKVLAQLSARKLLDSR-NH2). Ntawm lawv, CJC1295 siv D-amino acid, glycine dipeptide (Gly), disulfide choj thiab lwm yam kev hloov kho tshuaj los ua kom nws tsis kam mus rau gastric acid thiab enzymes, yog li txhim kho bioavailability thiab lub sij hawm ntawm cov tshuaj nyhuv.
8. Thermal stability:
CJC1295 tuaj yeem nyob ruaj khov hauv qee qhov kub thiab sijhawm. Cov kev tshawb fawb tau pom tias thaum CJC1295 yog yaj hauv dej ntshiab lossis ntsev, cov ntsiab lus poob tsuas yog li 1 feem pua tom qab cua sov ntawm 60 degree rau 30 feeb; thiab cov ntsiab lus poob yog li 10 feem pua tom qab cua sov ntawm 70 degree rau 30 feeb. Yog li ntawd, thaum lub sij hawm tsim khoom, kev cia khoom thiab kev thauj mus los, kev saib xyuas yuav tsum tau ua kom tsis txhob raug rau qhov kub thiab txias, kom tsis txhob cuam tshuam rau nws cov kev ua tau zoo.
9. pH stability:
Kev ua tau zoo ntawm CJC1295 yog ze ze rau tus nqi pH, thiab nws feem ntau ua rau tsis ua hauj lwm hauv acidic ib puag ncig. Cov txiaj ntsig kev sim qhia tau tias qhov ua tau zoo ntawm CJC1295 tau raug rhuav tshem tom qab tau yaj hauv hydrochloric acid tov nrog pH ntawm 2.0 tom qab 1 teev ntawm kev kho mob. Hauv cov tshuaj tsis haum nrog pH 7.4, CJC1295 muaj ntau dua kev lom zem thiab kev ruaj ntseg. Yog li ntawd, thaum siv CJC1295, koj yuav tsum xaiv qhov tsis muaj lossis cov khoom ntxiv uas ua tau raws li nws cov kev xav tau ntawm kev siv tshuaj, thiab tswj tus nqi pH.
10. Solubility thiab stability:
CJC1295 yog yooj yim soluble nyob rau hauv ob qho tib si dej thiab ethanol, tab sis nws solubility nyob rau hauv uas tsis yog-polar solvents yog kuj tsawg. Nyob rau hauv cov nqe lus ntawm nws stability, CJC1295 yog raug rau tej yam xws li oxidation, photolysis, thiab microbial paug, yog li yuav tsum tau saib xyuas kom tsis txhob ntev raug rau huab cua, tshav ntuj, los yog microbial ib puag ncig.
CJC-1295 muaj cov dej solubility tsawg thiab yuav tsum tau tsim los ntawm kev ntxiv cov kuab tshuaj tshwj xeeb. Kev npaj cov kev daws teeb meem muaj xws li anhydrous acetic acid, anhydrous methanol, PBS tsis, thiab lwm yam.
Rau cov saw hlau polypeptide ntawm CJC -1295 uas muaj DAC, nws muaj qhov zoo dua hauv dej solubility. Tom qab ua tiav lawm, CJC-1295 muaj DAC uas tuaj yeem diluted nrog physiological saline (PBS) los tsim ib qho kev daws teeb meem.
11: Spectral nta:
Lub infrared spectrum (IR) ntawm CJC1295 qhia tau hais tias qhov nqus peaks yog 3296 cm^-1, 3084 cm^-1, 2926 cm^-1, 1623 cm^-1 , 1538 cm^-1, 1395 cm^-1, 1329 Cov haujlwm ntawm cm^-1 thiab 1250 cm^-1 ua raws li cov yam ntxwv ntawm peptide molecules. Nws cov ultraviolet spectrum (UV) muaj qhov pom tseeb qhov siab tshaj plaws ntawm 280 nm, qhia tias nws muaj cov ntxhiab amino acid residues xws li tyrosine thiab phenylalanine. Lub nuclear magnetic resonance (NMR) spectrum ntawm CJC1295 tau pom cov qauv teeb liab nyuaj, qhia txog cov ncauj lus kom ntxaws ntawm nws cov qauv peptide.
Raws li GHRH analogue, CJC-1295 muaj kev kho mob dav dav thiab tsis yog qhov chaw kho mob. Nkag siab txog cov khoom reactivity ntawm CJC-1295 thiab nkag siab txog kev sib cuam tshuam ntawm nws cov qauv molecular thiab kev ua lom lom yog qhov tseem ceeb thaum lub sij hawm tsim tshuaj. Nrog rau kev nkag siab tob txog cov khoom muaj txiaj ntsig ntawm CJC-1295, cov kws tshawb fawb tuaj yeem tsim ntau cov bioactive thiab ruaj khov derivatives thiab formulations, yog li txhim kho nws daim ntawv thov kev cia siab hauv kev kho thiab tiv thaiv kab mob.