Bromophenol xiavyog lub teeb daj mus rau xim daj daj hmoov; Nws yog yooj yim soluble nyob rau hauv sodium hydroxide tov, soluble nyob rau hauv methanol, ethanol thiab benzene, me ntsis soluble nyob rau hauv dej (txog {{0}}.4g/100ml), thiab qhov siab tshaj plaws nqus wavelength yog 422nm. Nws yog qhov ntsuas pH. Hauv thaj tsam ntawm pH 3.0 ~ 4.6, xim hloov ntawm daj mus rau xiav; Nws kuj yog feem ntau siv los ua electrophoretic taw qhia dye. Lub electrophoretic migration tus nqi nyob rau hauv lub gel yog nyob rau hauv lub cheeb tsam ntawm me me molecules ntawm nucleic acid los yog protein. Thaum kawm txog kev sib cuam tshuam ntawm tib neeg hemoglobin thiab ntau yam tsiaj hemoglobin (ua luv raws li kev sib cuam tshuam), nws tau pom tias bromophenol xiav (BPB) tuaj yeem cuam tshuam nrog nqaij qaib hemoglobin hauv cov txheej txheem hla electrophoresis, tab sis qhov tshwm sim no tsis muaj nyob ntawm BPB thiab cov neeg laus. hemoglobin. BPB txawv ntawm hemoglobin. Nws yog ib qho qis molecular compound. Nws qhov kev cuam tshuam nrog hemoglobin tuaj yeem sib txawv los ntawm kev sib cuam tshuam ntawm hemoglobin hauv cov txheej txheem.
Piv txwv ntawm kev siv bromophenol xiav yog raws li nram no:
1. Lub photochemical composite rhiab membrane rau hydrogen ion paub tau raug npaj. Cov kauj ruam ntawm txoj kev yog raws li nram no:
1) Ua ntej, yaj 55g acrylamide nyob rau hauv 20-30ml distilled dej, lim nws, ces txias cov lim dej rau hauv chav tsev kub, ces ua nqus pom, tos kom nws crystallize thiab qhuav lawm, nqus nws kom tau ntshiab acrylamide. ;
2) Dissolve 10g ntawm ntshiab acrylamide, 0. N, N methylene acrylamide, 0.01-0.012g ntawm cresol violet thiab 0. ntawm cresol violet thiab bromophenol xiav;
3) Lub centrifuge high-speed centrifuge yog siv los tig lub polymer los ua ib qho photochemical composite rhiab zaj duab xis. Lub invention muaj cov txheej txheem yooj yim, tus nqi qis thiab cov lus teb zoo, cov yam ntxwv kub thiab rov ua dua ntawm cov yeeb yaj kiab uas tau npaj. Nws tuaj yeem nthuav dav ntau yam rhiab heev rau hydrogen ions, thiab tuaj yeem siv los tsim qhov rhiab ntawm qhov lees paub ntawm hydrogen ion photochemical sensor.
2. Cov hmoov protein sib xyaw nrog calcium ntxiv muaj nuj nqi yog npaj, uas suav nrog cov khoom siv raw thiab cov khoom hnyav hauv qab no: almond ncuav mog qab zib suav rau 30-40 feem pua ntawm tag nrho pawg; Acetone suav rau 1.5 ~ 2.5 feem pua ntawm tag nrho cov huab hwm coj; Qhov feem ntawm sodium bicarbonate tov nyob rau hauv tag nrho cov loj yog 8-15 feem pua ; Hydrochloric acid suav rau 1.5 ~ 2.5 feem pua ntawm tag nrho cov huab hwm coj; Bromophenol xiav suav txog 1.5 ~ 2.5 feem pua ntawm tag nrho cov huab hwm coj; Qhov kev faib ua feem ntawm cov vitamin D nyob rau hauv tag nrho pawg yog 12-18 feem pua ; Calcium gluconate tso nyiaj rau 15-20 feem pua ntawm tag nrho pawg; Ethanol suav txog 1.5 ~ 2.5 feem pua ntawm tag nrho cov huab hwm coj thiab cov dej distilled suav txog 15 ~ 20 feem pua ntawm tag nrho cov huab hwm coj. Lub invention siv almond ncuav mog qab zib li raw khoom, rho tawm nrog acetone thiab ethanol, lim thiab dries los npaj protein hmoov, thiab siv calcium gluconate li calcium ntxiv. Cov hmoov sib xyaw protein ua los ntawm kev siv cov qauv no tsis tsuas yog txhim kho qhov pom kev zoo, tab sis kuj tuaj yeem txhim kho kev siv tau zoo thaum siv nrog cov protein thiab calcium gluconate, uas yog tsim rau cov neeg laus thiab cov laus uas muaj calcium tsis txaus.
3. Npaj cov khoom siv rau kev kwv yees qhov ua tau zoo ntawm sulfonylureas, uas muaj cov khoom nram qab no:
(1) Cov qe ntshav liab lysate;
(2) Leukocyte lysate;
(3) Protein nag lossis daus;
(4) Nucleic acid cia kua;
(5) PCR cov tshuaj tiv thaiv sib xyaw (muaj MgCl2, dNTP, PCR amplification primer thiab tus qauv sab hauv);
(6) DNA polymerase;
(7) Kev tswj kom zoo;
(8) Kev tswj tsis zoo;
(9) Endonuclease buffer system;
(10) Kev txwv endonuclease;
(11) PCR dej;
(12)10 × Sample loading buffer for electrophoresis: 0.25% bromophenol blue, 40% (w/v) sucrose aqueous tov. Nyob rau hauv txoj kev no, genomic DNA tau muab rho tawm los ntawm cov qauv tshuaj lom neeg, thiab cov polymorphism ntawm sulfonylurea receptor gene nyob rau hauv ib qho kev kuaj lom neeg tau kuaj pom los ntawm polymerase chain reaction restriction fragment polymorphism (PCR-RFLP) analysis to predict the efficiency of sulfonylurea drug.
Synthetic bromophenol xiav:
1. Dissolve phenol liab nyob rau hauv glacial acetic acid, ntxiv bromine tov nyob rau hauv glacial acetic acid nyob rau hauv stirring, do rau ob peb feeb, ncuav rau hauv dej kub 60 degrees, txias rau hauv chav tsev kub, thiab muab tso rau ib hmos. Lim, ntxuav lub ncuav mog qab zib nrog glacial acetic acid thiab benzene nyob rau hauv lem, thiab cua qhuav kom tau bromophenol xiav.
2. Dissolve phenol liab nyob rau hauv glacial acetic acid, kub nws mus rau boiling point, poob kua bromine yaj nyob rau hauv glacial acetic acid, lim thaum cov khoom daj sib cais, ntxuav dawb bromine nrog acetic acid, thiab qhuav nws nyob rau hauv huab cua kom tau cov khoom crude. Siv glacial acetic acid los yog acetone thiab glacial acetic acid tov cov kuab tshuaj kom recrystallize kom tau cov ntshiab bromophenol xiav.
3. Dissolve phenol liab thiab bromine hauv acetic acid los npaj cov tshuaj. Ua ntej, ua kom sov lub phenol liab acetic acid tov rau boiling point, ces maj mam ntxiv bromine acetic acid tov nyob rau hauv nplawm: thaum lub phenol liab cov tshuaj tiv thaiv tiav thiab cov daj muaju tsis sib cais, txias thiab lim. Tom qab ntxuav tawm cov dawb bromine hauv cov siv lead ua nrog me me ntawm acetic acid, recrystallize nws nrog acetic acid los yog acetone acetic acid, thiab qhuav nws nyob rau hauv huab cua kom tau ntshiab bromophenol xiav.