Trometamolyog ib qho organic tshuaj nrog cov tshuaj formula c4h11no3 thiab dawb crystalline hais. Nws tuaj yeem siv los ua cov khoom siv roj ntsha; Tsis siv neeg rau gel electrophoresis configuration. Raws li cov tshuaj alkaline, nws yog siv rau kev kho cov acidosis thiab tsis ua rau kom nce cov pa roj carbon dioxide retention. 7.28 feem pua tham 2-3ml / kg ( isotonic tov yog 3.64 feem pua ) txhua lub sij hawm, diluted nrog tib tus nqi ntawm 5 feem pua - 10 feem pua qabzib tov, thiab ces maj mam poob rau hauv. Tham yog cov tsiaj ntawv uas feem ntau siv. hauv tsev kho mob.
Kev siv ntawm trimethylol aminomethane:
1. Tris tsis yog tsuas yog siv los ua cov kuab tshuaj rau nucleic acids thiab cov proteins, tab sis kuj muaj ntau yam tseem ceeb siv.
2. Tris tau siv rau cov protein siv lead ua kev loj hlob raws li pH sib txawv.
3. Lub zog qis ionic ntawm Tris tsis tuaj yeem siv rau kev tsim cov fibers nruab nrab ntawm lamin hauv C. elegans.
4. Tris kuj yog ib qho tseem ceeb ntawm cov protein electrophoresis tsis khoom. Tsis tas li ntawd, Tris kuj yog ib qho nruab nrab hauv kev npaj cov surfactants, vulcanization accelerators thiab qee cov tshuaj. Tris kuj tau siv los ua tus qauv titration.
5. Cov yam ntxwv tsis zoo.
6. Tris yog lub hauv paus tsis muaj zog nrog pKa ntawm 8.1 ntawm chav tsev kub (25 degree); Raws li txoj kev xav tsis, qhov tsis zoo ntawm Tris tsis yog nyob nruab nrab ntawm pH 7.0 thiab 9.2.
7. Lub pH ntawm aqueous tov ntawm Tris puag yog hais txog 10.5. Feem ntau, hydrochloric acid yog ntxiv los kho tus nqi pH rau tus nqi xav tau kom tau txais qhov tsis txaus ntawm tus nqi pH no. Txawm li cas los xij, kev saib xyuas yuav tsum tau them rau cov txiaj ntsig ntawm qhov kub thiab txias ntawm pKa ntawm Tris.
Cov yam ntxwv tsis zoo
Tris yog lub hauv paus tsis muaj zog nrog pKa ntawm 8.1 ntawm chav tsev kub (25 degree); Raws li txoj kev xav tsis, qhov tsis zoo ntawm Tris tsis yog nyob nruab nrab ntawm pH 7.0 thiab 9.2.
Lub pH ntawm aqueous tov ntawm Tris puag yog hais txog 10.5. Feem ntau, hydrochloric acid yog ntxiv los kho tus nqi pH rau tus nqi xav tau kom tau txais qhov tsis txaus ntawm tus nqi pH no. Txawm li cas los xij, kev saib xyuas yuav tsum tau them rau cov txiaj ntsig ntawm qhov kub thiab txias ntawm pKa ntawm Tris.
Synthesis ntawm trimethylolaminomethane:
Txoj Kev 1:
(1) Ntxiv 800g ntawm industrial trimethylolmethane rau hauv 500ml ntawm aqueous methanol tov, tshav kub rau 60 degree, do thiab dissolve, nyob rau hauv lub aqueous methanol tov yog npaj los ntawm kev sib tov purified dej thiab methanol nyob rau hauv ib tug ntim ratio ntawm 2: 3;
(2) Ntxiv 8g ntawm charcoal activated carbon rau cov tshuaj, khaws cia ntawm 50 degree rau 30 feeb, ces lim nws thaum nws kub, thiab sau cov filtrate;
(3) Concentrate lub filtrate nyob rau hauv lub siab txo nyob rau hauv ib tug concentration kub ntawm 80 degree C kom txog thaum crystallization tshwm sim, txias nws down, thiab cov rov qab methanol tuaj yeem rov ua dua;
(4) Tom qab cov crystals raug cais los ntawm kev nqus pom, lawv tau yaug ob zaug nrog cov ethanol thiab qhuav ntawm 50 degree rau 4 teev kom tau 680g ntawm cov khoom tiav nrog purity ntawm 100.03 feem pua.
Cov khoom raug tshuaj xyuas raws li Q / 12hb4907-2013 tus qauv:
(1) Kev ntsuas dej solubility
Nqa 1 g ntawm qhov hnyav, ntxiv 10 ml dej, co zoo thiab yaj. Kev daws yuav tsum yog qhov tseeb thiab pob tshab.
(2) Kev kuaj xyuas qhov hluav taws kub
Qhov hnyav 5g ntawm cov qauv, muab tso rau hauv lub crucible nrog qhov hnyav tas li, cua sov thiab carbonize nws maj mam, txias nws, ntxiv 0.5ml ntawm sulfuric acid, txuas ntxiv cua sov kom txog thaum lub sulfuric acid vapor tag, thiab hlawv nws nyob rau hauv ib tug Lub qhov cub kub kub ntawm 650 ± 50 degree kom qhov hnyav tas li. Qhov seem seem yuav tsum tsis txhob ntau tshaj 1.0mg.
(3) Kev kuaj tshuaj chloride
Ntsuas 2G qauv, yaj nws hauv dej, dilute nws mus rau 20ml, ntxiv 2ml nitric acid tov (25 feem pua) thiab 1ml silver nitrate tov (17g / L), co nws, thiab tawm rau 10min. Lub turbidity yuav tsum tsis txhob ntau tshaj tus qauv.
Tus qauv yog kom dilute 0.01mg chloride impurity standard tov rau 20ml thiab kho nws nyob rau hauv tib txoj kev raws li cov qauv tov ntawm tib lub ntim.
(4) Sulfate xeem
Qhov hnyav 0.5g qauv, yaj hauv dej, dilute nws mus rau 20ml, ntxiv 1ml hydrochloric acid tov (20 feem pua) thiab 3ml barium chloride tov (250G / L), co nws zoo, thiab tso rau 20min. Lub turbidity yuav tsum tsis txhob ntau tshaj tus qauv.
Tus qauv yog kom dilute 0.02mg ntawm impurity sulfate txheem tov rau 20ml thiab kho nyob rau hauv tib txoj kev raws li cov qauv tov ntawm tib lub ntim.
(5) Kev tshuaj xyuas cov hlau hnyav
Qhov hnyav 4G ntawm cov qauv, yaj nws hauv dej, ntxiv acetic acid tov (30 feem pua) rau neutralize thiab dilute nws mus rau 40ml, noj 30ml, ntxiv 0.2ml acetic acid tov (30 feem pua) thiab 10ml tshiab npaj saturated hydrogen. sulfide dej, co zoo. Cov xim tsaus yuav tsum tsis txhob ntau dua li tus qauv.
Tus qauv yog kom dilute qhov seem 10ml ntawm cov qauv tov thiab 0.006mg ntawm impurity lead standard tov rau 30ml, thiab kho nws tib lub sij hawm raws li cov qauv tov ntawm tib lub ntim.
Txoj Kev 2:
1. Ua ntej, nitromethane thiab paraformaldehyde sib tov hauv ib qho piv txwv ntawm 1: 3.3. Ua ntej, 93g ntawm paraformaldehyde yog hnyav thiab muab tso rau hauv 500ml plaub caj dab hwj. Tom qab ntawd, 85g ntawm methanol hnyav thiab 0.3g ntawm KOH catalyst yog ntxiv, rhuab thiab nplawm kom txog thaum lawv yaj tag. Tom qab ntawd, 55g ntawm nitromethane maj mam ntxiv dropwise rau condensation cov tshuaj tiv thaiv, thiab cov tshuaj tiv thaiv kub yog khaws cia ntawm 40-55 degree . Tom qab ntxiv, nws tau khaws cia rau 3-4 teev (txog 55 degree ).
2. Thaum kawg ntawm cov tshuaj tiv thaiv, muab cov condensate tso rau hauv 1L autoclave hauv ib lub lauj kaub, ntxiv 350g methanol thiab 20g npib tsib xee catalyst, xyuas seb lub autoclave puas zoo li qub, hloov cov hydrogen hauv autoclave, hloov ntawm lub hwj huam mov, pib stirring , thiab txhaj tshuaj hydrogen kom txo cov tshuaj tiv thaiv. Lub siab yog khaws cia ntawm 2-3mpa, cov tshuaj tiv thaiv kub yog tswj ntawm 40-50 degree, hydrogenation yog khaws cia rau 2-3 teev (kwv yees li 50 degree), thiab qhov kub yog nce mus rau 60 degree rau 1h. Kev hloov pauv tus nqi yog 98 feem pua.
3. Pub cov khoom rau hauv 1000ml plaub caj dab, ntxiv 5g ntawm cov pa roj carbon activated, do rau 1 teev (kub qhov kub ntawm li 50 degree), lim nrog Buchner funnel, txias thiab crystallize 70g ntawm crude. khoom. Siv 70g ntawm cov khoom nyoos, ntxiv 65g dej deionized thiab 5g ntawm cov pa roj carbon activated rau hauv 500ml plaub lub caj dab, cua sov thiab do, kub rau 80 degree rau 1 teev, lim nws nrog Buchner funnel, txias nws, thiab ces tso tawm. . Qhuav nws kom tau 65g ntawm cov khoom tiav. Cov ntsiab lus ntawm cov khoom tiav yog 99.94 feem pua, thiab absorbance (260nm) yog 0.043. Cov ntsiab lus methanol ntawm cov tshuaj yog 99 feem pua.