Thiazolyl Blueyog ib qho organic compound nrog cov tshuaj formula C16H14ClN5S thiab molecular hnyav 353.84 g/mol. Nws tseem hu ua MTT (3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyltetrazolium bromide). Tsaus xiav khoom. Soluble nyob rau hauv dej, methanol, ethanol thiab dimethyl sulfoxide, insoluble nyob rau hauv non-polar solvents xws li n-heptane. Nws decomposes thaum raug lub teeb, yog li nws yuav tsum tau khaws cia rau hauv qhov chaw tsaus, qhuav.
Thiazolyl Blue yog tetrazole compound nrog qee yam tshuaj tshwj xeeb. Cov nram qab no yog cov khoom siv tshuaj ntawm Thiazolyl Blue:
1. Cov tshuaj tiv thaiv Redox tuaj yeem tshwm sim: Thiazolyl Blue tuaj yeem hloov mus rau lwm cov tshuaj nyob rau hauv cov xwm txheej redox sib txawv. Piv txwv li, nws tuaj yeem raug txo los ntawm cov enzymes hauv mitochondrial respiratory saw raws li cov neeg txais los ntawm kev ua raws li lub substrate nrog NADH los tsim cov dej-soluble ntshav cov khoom.
2. Ncua rau catalyst nkaus: Thiazolyl Blue tuaj yeem siv los ua cov khoom siv rau tooj liab ions los ua kom muaj kev cuam tshuam ntau yam xws li oxidation ntawm ferrous oxide thiab aldehyde compounds.
3. tuaj yeem tsim cov complexes nrog cov hlau ions: Sulfur, nitrogen thiab lwm yam atoms hauv Thiazolyl Blue tuaj yeem tsim cov complexes nrog hlau ions, xws li tooj liab complexes, yog li cuam tshuam rau nws nqus thiab fluorescence zog.
4. Nws tuaj yeem decolorized nyob rau hauv muaj zog alkaline tej yam kev mob: Thiazolyl Blue yuav decolorize nyob rau hauv muaj zog alkaline tej yam kev mob thiab tsim ib tug colorless khoom.
Yuav kom suav tau, cov khoom siv tshuaj ntawm Thiazolyl Blue yog qhov nyuaj thiab muaj ntau haiv neeg, thiab cov khoom no ua lub luag haujlwm tseem ceeb hauv nws daim ntawv thov.
Txoj kev hluavtaws ntawm Thiazolyl Blue feem ntau suav nrog cov hauv qab no:
1. Griess-Reagent txoj kev:
Txoj kev Griess-Reagent yog ib qho ntawm cov txheej txheem hluavtaws thaum ntxov ntawm Thiazolyl Blue, thiab nws txoj kev hluavtaws suav nrog cov kauj ruam hauv qab no:
(1) react phenylsulfonyl chloride thiab 1, 2-ethylenediamine kom tau N, N'-diphenylsulfonyl-1, 2-ethylenediamine;
(2) React N, N'-diphenylsulfonyl-1, 2-ethylenediamine thiab nitroaniline nyob rau hauv alkaline tej yam kev mob los tsim 2,3-dimethyl-5-nitro-4-phenyl Thiazoline (TNBT);
(3) Txo TNBT rau 2, 3-dimethyl-5-methoxycarbonyl-4-phenylthiazoline (MTT).
Ntawm lawv, Griess-Reagent yog ib qho sib tov siv los ua tus neeg sawv cev txo qis hauv cov txheej txheem tshuaj tiv thaiv, thiab nws cov muaj pes tsawg leeg suav nrog sodium nitrite thiab sulfuric acid. Lub luag haujlwm ntawm Griess-Reagent yog txo cov nitrobenzoic acid rau nitrobenzophenone thiab txhawb kev tsim ntawm MTT intermediate. Thaum lub sij hawm cov txheej txheem tshuaj tiv thaiv, Griess-Reagent kuj plays lub luag hauj lwm nyob rau hauv kev kho tus nqi pH kom muaj pH tsim nyog ntawm cov tshuaj tiv thaiv.
Nws yuav tsum tau muab sau tseg tias cov tshuaj tiv thaiv tej yam kev mob nyob rau hauv lub Griess-Reagent txoj kev yuav tsum tau ua tib zoo tswj, thiab tsis xws li cov tshuaj tiv thaiv kub, pH nqi thiab cov tshuaj tiv thaiv lub sij hawm yuav cuam tshuam cov nyhuv ntawm cov tshuaj tiv thaiv thiab purity ntawm cov khoom. Tsis tas li ntawd, txij li Griess-Reagent muaj cov tshuaj lom xws li sodium nitrite, cov kev cai nruj kev nyab xeeb yuav tsum tau ua raws li thaum lub sijhawm ua haujlwm.
2. WST-1 txoj kev:
Txoj kev WST-1 yog ib txoj hauv kev txhim kho hluavtaws ntawm Thiazolyl Blue, uas feem ntau suav nrog cov kauj ruam hauv qab no:
(1) react benzaldehyde thiab thiourea nyob rau hauv alkaline tej yam kev mob kom tau N-benzylthiourea (BSMT);
(2) React BSMT thiab nitrobenzene nyob rau hauv cov xwm txheej yooj yim los tsim 2-(4-methoxycarbonylphenyl)-4,5-dimethylthiazoline (WST-1).
3. MTS txoj kev:
Txoj kev MTS yog lwm txoj kev txhim kho hluavtaws ntawm Thiazolyl Blue, thiab nws txoj kev hluavtaws muaj xws li cov kauj ruam hauv qab no:
(1) React thiourea thiab benzaldehyde nyob rau hauv alkaline tej yam kev mob los tsim N-benzylthiourea (BSMT);
(2) React BSMT thiab aniline nyob rau hauv alkaline tej yam kev mob los tsim MTS.
4. MTT txoj kev:
Txoj kev MTT yog ib txoj hauv kev yooj yim tshaj plaws rau Thiazolyl Blue, uas feem ntau suav nrog cov kauj ruam hauv qab no:
(1) React nitrobenzene thiab 1,2-ethylenediamine nyob rau hauv alkaline tej yam kev mob los tsim 2,3-dimethyl-5-nitro-4-phenylthiazoline (TNBT);
(2) Txo TNBT rau 2, 3-dimethyl-5-methoxycarbonyl-4-phenylthiazoline (MTT).
Txhawm rau ua kom tiav, txoj kev sib txuas ntawm Thiazolyl Blue yog ntau haiv neeg, cov uas txoj kev Griess-Reagent thiab WST-1 txoj kev yog siv cov txheej txheem hluavtaws ntau dua, thaum txoj kev MTS thiab MTT txoj kev yog qhov yooj yim.