Earthworm Peptide

Cov txheej txheem tsim khoom pib nrog kev tswj xyuas me ntsis thermal kho cov kauj ruam. Cov theem no yog tsim los ua kom muaj kev nthuav tawm ib feem ntawm cov txheej txheem protein nyob hauv tertiary thiab quaternary yam tsis ua rau ntau dhau denaturation lossis degradation. Los ntawm kev so kom txaus siab dua - txiav txim kom muaj protein ntau, cov ntawv cog lus hauv peptide thiab cov chaw enzymatic cleavage tau nkag tau yooj yim dua, yog li txhim kho qhov ua tau zoo ntawm cov enzymatic hydrolysis thiab txo cov tshuaj tiv thaiv kev sib txawv.

Tom qab thermal pretreatment, ib tug multi-enzyme system tsim los ntawm xaiv proteases nrog ntxiv cleavage tshwj xeeb yog qhia. Qhov no enzymatic system enables ib theem thiab tswj hydrolysis txheej txheem, maj mam zom cov protein ntau chains rau hauv me me peptide fragments. Cov xwm txheej hydrolysis-xws li kub, pH, enzyme concentration, thiab lub sij hawm tshuaj tiv thaiv- tau ua tib zoo tswj xyuas kom ntseeg tau tias qib ntawm hydrolysis nyob rau hauv ib lub hom phiaj, tiv thaiv ob qho tib si hydrolysis tsis txaus (uas yuav khaws tau siab -molecular{6}} hnyav proteins) thiab tshaj -hydrolysis (peptide) uas tuaj yeem ua kom muaj zog.

Thaum cov tshuaj tiv thaiv enzymatic mus txog qhov kawg xav tau, enzyme inactivation tiav los ntawm kev sib xyaw pH hloov thiab thermal deactivation. Cov kauj ruam no yog ib qho tseem ceeb kom stabilize peptide muaj pes tsawg leeg thiab tiv thaiv ntxiv degradation uncontrolled. Cov hydrolyzate yog tom qab ntawd raug rau cov khoom sib cais-kua kom tshem tawm cov khoom seem tsis txaus, suav nrog cov khoom noj protein uas tsis muaj kev cuam tshuam thiab tsis yog -solved organic teeb meem. Cov kua peptide clarified undergoes membrane fractionation purification, uas sawv cev rau cov kauj ruam tseem ceeb hauv kev tswj qhov hnyav molecular. Los ntawm kev xaiv filtration thiab fractionation, peptide pej xeem raug cais raws li molecular loj faib.

Cov txheej txheem no tso cai rau kom muaj txiaj ntsig ntawm qis-molecular- hnyav peptide feem ntau thaum txo qhov kev faib ua feem ntawm siab -molecular- hnyav residues thiab tsis yog -functional macromolecules. Raws li qhov tshwm sim, tag nrho cov peptide profile ua zoo ib yam thiab zoo ib yam nyob rau hauv ntau lawm batch, ho txhim kho reproducibility. Cov tshuaj purified peptide tom qab ntawd yog cov concentrated nyob rau hauv kev tswj xyuas uas siv ntau -cov nyhuv evaporation technology, uas txo qis thermal degradation thaum nce cov khoom khib nyiab. Thaum kawg, cov tshuaj concentrated yog hloov mus rau hauv cov hmoov ruaj khov ntawm cov tshuaj tsuag kom qhuav. Cov txheej txheem ziab no ua kom muaj kev tshem tawm sai sai thaum khaws peptide kev ncaj ncees thiab tswj xyuas cov yam ntxwv zoo solubility.

Cov khoom lag luam yog tsim los ntawm kev sib xyaw ua ke ntawm qis -molecular- hnyav peptide feem uas tau los ntawm kev tswj hwm enzymatic hydrolysis. Cov neeg peptide no suav nrog ntau pab pawg muaj txiaj ntsig uas tau tshaj tawm hauv cov ntaub ntawv tshawb fawb kom cuam tshuam nrog ntau txoj hauv kev lom neeg, txawm hais tias txhua yam haujlwm tseem nyob rau theem tshawb fawb.

Ntawm cov peptide feem uas tau txheeb xyuas yog angiotensin-hloov enzyme (ACE)-inhibitory peptides, uas yog txuam nrog cov qauv kev sim nrog kev hloov pauv ntawm ntshav siab-txog txoj hauv kev enzymatic. Tsis tas li ntawd, cov tshuaj tiv thaiv antioxidant- ntsig txog peptide ib ntus tau pom los koom nrog hauv kev tswj hwm ntawm oxidative stress markers, uas muaj peev xwm ua rau muaj kev cuam tshuam dawb radical scavenging nyob rau hauv vitro.

Earthworm peptidekuj tseem muaj cov peptide feem uas tau tshawb xyuas rau lawv lub luag haujlwm hauv kev tiv thaiv kab mob, tshwj xeeb yog los ntawm kev cuam tshuam nrog kev ua haujlwm macrophage thiab inflammatory cytokine qhia, suav nrog TNF- thiab IL-6. Tsis tas li ntawd, qee qhov peptide Cheebtsam txuas nrog kev tswj hwm lipid metabolism thiab plab hnyuv microbiota tshuav nyiaj li cas hauv cov qauv kev tshawb fawb preclinical, qhia txog ntau txoj hauv kev lom neeg muaj feem cuam tshuam ntawm qib kev sim.

Lumbricusin raug txheeb xyuas raws li tus cwj pwm peptide hauv cov kab mob hauv av- muab los ntawm peptide systems. Nws yog dav siv nyob rau hauv kev tshawb fawb kev kawm raws li ib tug neeg sawv cev biomolecule rau tracing thiab characterizing peptide profiles muab tau los ntawm earthworm protein hydrolysates. Nyob rau hauv cov ntsiab lus no, nws ua hauj lwm feem ntau raws li ib tug qauv thiab analytical reference indicator es tsis yog ib tug active tshuaj muaj.

Ntxiv nrog rau peptide feem, lub kaw lus khaws cia cov khoom sib txuas ua ke tau los ntawm cov khoom siv roj ntsha qub. Cov no suav nrog Lysenin- cuam tshuam txog cov tshuaj tiv thaiv kab mob peptide fragments, cov amino acids dawb, tshwm sim alkaloids, thiab cov ntxhia ntxhia. Kev sib koom ua ke ntawm cov khoom no tsim cov txheej txheem bioactive matrix, uas tuaj yeem ua rau muaj peev xwm sib koom ua ke ntawm cov qauv tsim, txawm hais tias cov kev sib cuam tshuam no tseem raug tshawb fawb ntxiv.

Cov lus ceeb toom ntxiv: Lysenin yog ib qho sphingomyelin -binding pore- tsim cov protein uas yog tsim los ntawm hom kab mob av. Hauv kev tswj hwm hauv vitro kev sim tshuab, nws tau pom tias muaj kev cuam tshuam nrog cov txheej txheem lipid membrane thiab tuaj yeem ua rau cov membrane permeability ntawm erythrocytes ntawm qee qhov kev ua haujlwm siab, uas ua rau muaj kev ua haujlwm hemolytic. Yog li ntawd, nws lub xub ntiag yuav tsum tau ua tib zoo xav thaum lub sij hawm tsim qauv, thiab nws cov ntsiab lus yuav tsum raug soj ntsuam kom tsim nyog thiab tswj tau raws li qhov xav tau ntawm daim ntawv thov scenario.

Txhua qhov kev tshaj tawm txog kev lom neeg cuam tshuam nrog cov kab mob peptide no tsuas yog muab los ntawm hauv vitro cell-raws li kev sim thiab hauv vivo kev tshawb fawb tsiaj. Cov kev tshawb pom no sawv cev thaum ntxov- cov txiaj ntsig kev tshawb fawb txog theem pib thiab yuav tsum tau txhais nruj me ntsis hauv cov ntsiab lus ntawm kev tshawb fawb preclinical. Tam sim no tsis muaj cov ntaub ntawv pov thawj ntawm tib neeg cov ntaub ntawv kho mob kom paub meej txog kev ua tau zoo ntawm lub cev lossis cov txiaj ntsig kho mob.

Hauv cov kab mob hauv lub cev -cov qauv kev tshawb fawb txog, qee yam qis-molecular- hnyav peptide feem ntau pom tau tias muaj peev xwm koom nrog hauv txoj hauv kev ntawm fibrin degradation. Kev soj ntsuam kev soj ntsuam qhia tias muaj peev xwm cuam tshuam rau cov ntshav rheology tsis thiab microcirculation- cov cim qhia, feem ntau los ntawm kev cuam tshuam nrog fibrinolytic system Cheebtsam. Txawm li cas los xij, nws yog ib qho tseem ceeb uas yuav tsum nco ntsoov tias kev ua haujlwm fibrinolytic ntawm enzymatically muab tau los ntawm peptide feem yog qis dua li ntawm cov kab mob hauv av lumbrokinase enzymes, uas yog cov qauv sib txawv thiab ua haujlwm ntau dua hauv fibrin degradation.

Hauv kev tshawb fawb txog kev tiv thaiv kab mob thiab kab mob inflammatory, peptide Cheebtsam tau pom los hloov kho macrophage phagocytic kev ua si thiab cuam tshuam cov theem ntawm cov pro-inflammatory cytokines, nrog rau cov qog necrosis factor alpha (TNF-) thiab interleukin-6 (IL-6). Cov kev tshawb pom no qhia tias muaj peev xwm koom nrog hauv txoj hauv kev tiv thaiv kab mob tiv thaiv kab mob, tshwj xeeb tshaj yog nyob rau hauv kev tswj hwm qhov sib npaug hauv kev sim siab.

Hauv kev tshawb nrhiav antioxidant thiab kev tiv thaiv cellular, lub peptide system tau ua kom pom cov dawb radical scavenging muaj peev xwm hauv tshuaj lom neeg thiab kev soj ntsuam ntawm tes. Cov khoom no tuaj yeem ua rau txo qis oxidative kev nyuaj siab-induced cellular puas nyob rau hauv vitro, txhawb nws nquag siv raws li cov ntaub ntawv tshawb fawb nyob rau hauv kev tshawb fawb kev tshawb fawb txog kev tiv thaiv ntawm daim tawv nqaij barrier, cellular stress teb, thiab superficial ntaub so ntswg kho qauv.

Vim nws qhov hnyav molecular peptide muaj pes tsawg leeg thiab zoo heev aqueous solubility,earthworm peptidetau ua tiav daim ntawv thov kev lag luam uas paub tab nyob rau hauv cov txheej txheem kev xaiv, tshwj xeeb tshaj yog nyob rau hauv cov khoom noj khoom haus thiab kev tsim kho tus kheej. Hauv kev siv cov khoom noj khoom haus thiab kev noj zaub mov ntxiv, cov khoom xyaw tuaj yeem muab tso rau hauv cov khoom siv dej haus, cov qauv hloov zaub mov, thiab cov khoom noj khoom haus kis las.

Nws ua haujlwm zoo li bioavailable peptide-raws li nitrogen qhov chaw tsim los ua kom zoo dua cov khoom noj muaj protein ntau. Hauv cov txheej txheem tsim, nws yog siv los txhawb kev txhim kho kev zom zaub mov thiab kev nqus ntawm cov protein, tshwj xeeb tshaj yog nyob rau hauv cov protein-cov khoom noj khoom haus muaj zog uas txhim kho bioavailability yog lub hom phiaj tseem ceeb ntawm kev tsim. Hauv kev saib xyuas tus kheej thiab kev siv tshuaj pleev ib ce, lub peptide system nthuav tawm cov yam ntxwv ntawm amphiphilic molecular, ua rau ib feem ntawm kev ua haujlwm ntawm lub ntsej muag uas yuav ua rau muaj kev txhawb nqa emulsification thiab formulation stabilization hauv aqueous systems.

Nyob rau hauv kev sib xyaw nrog pom cov tshuaj tiv thaiv antioxidant thiab kev tiv thaiv ntawm tes los ntawm kev tshawb fawb hauv vitro, cov khoom siv yog tsim rau kev suav nrog hauv soothing, moisturizing, thiab kho cov tawv nqaij formulations xws li serums, emulsions, thiab cream systems. Hauv kev ua haujlwm ntawm biomaterials thiab cov khoom siv qib siab, cov ntawv thov tam sim no tseem nyob rau theem kev tshawb fawb hauv chav kuaj. Kev tshawb nrhiav feem ntau yog tsom rau kev txhim kho hydrophilicity, kev hloov pauv ntawm cov qauv, thiab kev ruaj ntseg tawg hauv dej-raws li polymer systems. Tam sim no, tsis muaj kev lag luam loj - kev ua lag luam loj lossis kev lag luam khoom lag luam tau tsim nyob rau hauv qhov chaw no.