Shaanxi BLOOM Tech Co., Ltd. yog ib qho ntawm cov tuam ntxhab thiab cov muag khoom ntawm polymyxin b hmoov cas 1404-26-8 hauv Suav teb. Txais tos rau cov lag luam wholesale bulk zoo polymyxin b hmoov cas 1404-26-8 muag ntawm no los ntawm peb lub hoobkas. Kev pabcuam zoo thiab tus nqi tsim nyog muaj.
Polymyxin B hmoov, feem ntau yog tsim los ntawm polymyxin B sulfate, yog tshuaj tua kab mob macrocyclic. Nws cov tsos yog dawb los yog daj me ntsis amorphous hmoov, kuj piav raws li tsis muaj xim los yog daj crystalline hmoov, yuav luag odorless, yooj yim soluble nyob rau hauv dej, me ntsis soluble nyob rau hauv methanol, thiab insoluble nyob rau hauv acetone. Ntawm 25 ° C, nws solubility hauv dej yog 33.33 mg / mL. Nws feem ntau khi nrog kev sib raug zoo siab rau LPS (lipopolysaccharides) ntawm phab ntsa kab mob, rhuav tshem cov kab mob Gram tsis zoo, ua rau lawv cov permeability, thiab ua rau cov kab mob tuag taus. Nws kuj tseem tuaj yeem ua rau cov tshuaj endotoxins thiab tuaj yeem siv rau hauv kev tshawb fawb txog endotoxemia.
Nws yog siv los kho cov kab mob urinary, sepsis, hlawv qhov txhab, thiab meningitis tshwm sim los ntawm Gram tsis zoo cov kab mob xws li Pseudomonas aeruginosa. Polymyxin B kuj muaj qee yam kev kho mob ntawm sepsis thiab meningitis tshwm sim los ntawm kev siv tshuaj tua kab mob resistant Gram tsis zoo xws li Escherichia coli thiab Klebsiella pneumoniae. Hauv kev tshawb fawb txog kev tshawb fawb, nws kuj tseem siv tau rau cov kev tshawb fawb ntsig txog xws li endotoxemia.
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Kev siv ntawmpolymyxin B hmoovFeem ntau yog tshwm sim hauv nws daim ntawv thov tshuaj ua tshuaj tua kab mob.
Antibacterial nyhuv
Cov tshuaj no tsuas yog tsom rau cov kab mob Gram tsis zoo, suav nrog Escherichia coli, Pseudomonas aeruginosa, Escherichia coli, Klebsiella pneumoniae, Acinetobacter baumannii, nrog rau qee cov Proteus thiab Salmonella. Cov kab mob no feem ntau ua rau muaj ntau yam kab mob, xws li kab mob urinary, kab mob ua pa, kab mob hauv plab, kub hnyiab, thiab sepsis.
Nws muaj inhibitory los yog cov kab mob bactericidal thiab tseem yog tshuaj tua kab mob peptide tiv thaiv kab mob Gram tsis zoo. Nws khi rau lipopolysaccharide (LPS) ntawm cov kab mob cell membrane nrog kev sib raug zoo siab, hloov cov txheej txheem ntawm daim nyias nyias, thiab ua rau cov khoom me me kom xau, yog li inhibiting kev loj hlob ntawm Gram negative bacteria.
Kev kho cov kab mob hnyav: Polymyxin B feem ntau yog siv los kho cov kab mob hnyav uas tshwm sim los ntawm cov kab mob Gram tsis zoo uas tau hais tseg, tshwj xeeb tshaj yog thaum lwm cov tshuaj tua kab mob tsis zoo lossis txhim kho kev tiv thaiv. Nws tuaj yeem siv tau los ntawm kev txhaj tshuaj rau hauv cov hlab ntsha, kev txhaj tshuaj intramuscular, lossis daim ntawv thov hauv zos, thiab cov kev tswj hwm tsim nyog thiab ntau npaum li cas tuaj yeem raug xaiv raws li qhov chaw thiab qhov hnyav ntawm tus kab mob.
Kev sib xyaw ua ke: txhawm rau txhim kho kev kho mob kom zoo thiab txo qis kev txhim kho ntawm cov tshuaj tiv thaiv, polymyxin B feem ntau siv ua ke nrog lwm cov tshuaj tua kab mob. Qhov kev sib xyaw ua ke no tuaj yeem nthuav dav cov kab mob tua kab mob, txhim kho cov kab mob bactericidal, thiab txo qhov tshwm sim ntawm qhov tshwm sim tsis zoo.
Daim ntawv thov hauv zos: Ntxiv nrog rau kev siv lub cev, polymyxin B kuj tuaj yeem siv rau hauv cheeb tsam rau cov kab mob, xws li qhov txhab kub hnyiab, mob qhov muag, kab mob ntawm daim tawv nqaij thiab cov nqaij mos, thiab lwm yam.
Kev siv tshuaj kho mob
Cov kab mob hauv lub cev: Polymyxin B muaj cov tshuaj tua kab mob tseem ceeb ntawm ntau yam kab mob Gram tsis zoo, thiab yog li ntawd feem ntau siv los kho cov kab mob urinary los ntawm cov kab mob no, xws li cystitis, pyelonephritis, thiab lwm yam.
Cov kab mob ua pa: Polymyxin B kuj muaj qee yam kev kho mob ntawm cov kab mob ua pa los ntawm cov kab mob Gram tsis zoo, xws li mob ntsws, mob ntsws, thiab lwm yam.
Cov kab mob plab hnyuv: Cov hnyuv yog ib qho ntawm cov chaw kis kab mob rau cov kab mob Gram tsis zoo, thiab polymyxin B tuaj yeem siv los kho cov kab mob hauv plab los ntawm cov kab mob no, xws li kab mob enteritis, kab mob dysentery, thiab lwm yam.
Kab mob kub hnyiab: Tom qab kub hnyiab, qhov txhab raug cuam tshuam los ntawm cov kab mob Gram tsis zoo, ua rau muaj kab mob. Polymyxin B tuaj yeem siv hauv zos los hlawv qhov txhab los tiv thaiv thiab kho kab mob.
Lwm yam kab mob: Ntxiv nrog rau cov lus qhia tau hais los saum no, polymyxin B kuj tuaj yeem siv los kho lwm yam kab mob uas tshwm sim los ntawm gram- cov kab mob tsis zoo, xws li kab mob plab, kab mob hauv plab, sepsis, thiab lwm yam.
Intravenous txhaj: Rau cov neeg mob uas muaj kab mob loj heev los yog cov neeg uas xav tau kev kho mob sai, cov tshuaj txhaj tshuaj tuaj yeem siv rau kev tswj hwm. Cov koob tshuaj feem ntau yog txiav txim siab raws li cov xwm txheej xws li tus neeg mob qhov hnyav, qhov chaw kis kab mob, thiab qhov hnyav.
Kev txhaj tshuaj Intramuscular: Kev txhaj tshuaj Intramuscular yog lwm txoj kev tswj hwm ntawm polymyxin B, haum rau cov neeg mob uas muaj tus kab mob me me mus rau nruab nrab lossis cov neeg uas tsis tuaj yeem tiv thaiv kev txhaj tshuaj. Cov koob tshuaj kuj yuav tsum tau txiav txim siab raws li qhov tshwj xeeb ntawm tus neeg mob.
Daim ntawv thov hauv zos: Rau cov kab mob hauv zos xws li kab mob hlawv, polymyxin B tuaj yeem siv ncaj qha rau thaj chaw cuam tshuam los tiv thaiv thiab kho cov kab mob.
Mechanism of Action ntawm Polymyxin B
Lub hom phiaj tshwj xeeb ntawm cov kab mob sab nrauv Membrane Architecture
Polymyxin B hmoovyog ib qho tshuaj tua kab mob polypeptide muaj zog tiv thaiv Gram- cov kab mob tsis zoo, nrog rau nws lub hom phiaj tseem ceeb molecular yog lipopolysaccharide (LPS) nyob rau hauv daim nyias nyias ntawm Gram- cov kab mob tsis zoo. Nws cov molecule nqa ntau yam zoo diaminobutyric acid residues, conferring muaj zog cationic zog nyob rau hauv physiological pH tej yam kev mob. Nws xaiv khi rau lipid Ib moiety ntawm LPS ntawm electrostatic kev sib cuam tshuam.
Cov qauv kev ruaj ntseg ntawm Gram-negative daim nyias nyias nyob ntawm divalent cations xws li magnesium thiab calcium, uas tsim cross-txuas ntawm LPS molecules uas nyob ib sab. Polymyxin B sib tw tshem tawm cov cations no, ncaj qha destabilizing lub txheej membrane thiab cuam tshuam nws permeability - qhov no yog qhov tseem ceeb thawj kauj ruam ntawm nws cov tshuaj tua kab mob. Raws li hom phiaj ntawm qhov kev txiav txim no, nws nthuav tawm cov tshuaj tua kab mob tsis zoo tiv thaiv Gram- cov kab mob zoo uas tsis muaj LPS- muaj cov kab mob sab nrauv, muab nws cov kab mob tshwj xeeb tshwj xeeb.
Kev cuam tshuam ntawm Cytoplasmic Membrane Integrity Ua rau Bacterial Lysis thiab tuag
Tom qab khi rau cov kab mob sab nrauv, cov kab mob hydrophobic fatty acid sab saw ntawm polymyxin B ntxig rau hauv lipid bilayer ntawm cov kab mob cytoplasmic daim nyias nyias, disorganizing qhov kev txiav txim ntawm daim nyias nyias lipids thiab inducing tsim ntawm irregular transmembrane pores. Cov pores no nkag mus rau ob sab hauv thiab sab nrauv cov kab mob, ua rau muaj qhov loj heev ntawm intracellular nucleotides, electrolytes thiab qis -molecular- hnyav metabolites, thaum ua kom cov tshuaj lom exogenous infiltrate cov kab mob cytoplasm.
Intracellular osmotic tshuav nyiaj li cas ntawm cov kab mob vau tag, thiab cov txheej txheem physiological tseem ceeb xws li lub zog metabolism thiab biomolecule biosynthesis tsis ua haujlwm li qub, thaum kawg ua rau cov kab mob lysis thiab tuag. Cov kab mob bactericidal no yog qhov concentration- nyob ntawm: cov tshuaj ntau dua ua rau muaj kev puas tsuaj rau daim nyias nyias ntau dua thiab tua kab mob sai dua, nrog rau qhov tsis zoo rau qhov tshwm sim ntawm cov mutants resistant.
Neutralization ntawm Endotoxins rau Augment Antibacterial Effects
Tshaj li cov kab mob bactericidal ncaj qha, polymyxin B khi dawb LPS endotoxins los thaiv endotoxin -kev ua haujlwm nruab nrab ntawm tus tswv tsev inflammatory signaling cascades, mitigating inflammatory storms thiab toxic reactions induced by Gram-negative bacterial infections.
Nws cationic molecular molecular molecular nruj encapsulates lub active epitopes ntawm endotoxins thiab suppresses toxin paub thiab downstream cov lus teb. Thaum siv los tiv thaiv- kis kab mob, nws txo qis cov ntaub so ntswg puas tsuaj los ntawm cov kab mob sib kis- qhov no sawv cev rau qhov zoo tshaj plaws hauv kev kho mob rau kev kho mob hnyav los ntawm ntau yam tshuaj -tiv taus Gram- cov kab mob tsis zoo.
Core Production Process ntawm Microbial Fermentation
Fermentation siv Bacillus strains ua haujlwm tseem ceeb hauv kev lag luam thiab kev sim - nplai kev tsim qauv rau polymyxin B, nrog Bacillus polymyxa ua cov hom tseem ceeb uas tsim los ntawm biosynthesizes polymyxin B complex.
Ua ntej fermentation, ib tug kua kab lis kev cai nruab nrab uas muaj piam thaj, pob kws steep cawv thiab inorganic ntsev yog npaj, hloov mus rau ib tug pH ntau ntawm 7.0-7.5, thiab sterilized ntawm kub kub ua ntej inoculation nrog activated noob kab lis kev cai. Kev tswj qhov kub thiab txias ntawm 30-32 degree, tsis tu ncua aeration thiab agitation yog khaws cia thoob plaws hauv fermentation kom muaj peev xwm yaj cov pa oxygen txaus thiab ib puag ncig zoo metabolic rau microbial proliferation.
Lub voj voog tag nrho fermentation kav 3 mus rau 5 hnub. Thaum lub sij hawm lub logarithmic kev loj hlob theem, lub strain synthesizes ntau ntau ntawm polymyxin B thiab secretes nws extracellularly, yielding fermentation broth enriched nrog siab concentrations ntawm lub hom phiaj cov khoom. Cov txheej txheem biosynthetic no ua haujlwm nyob rau hauv cov tshuaj tiv thaiv me me thiab xa tawm siab -cov khoom lag luam bioactivity, ua rau nws txoj hauv kev tseem ceeb rau kev lag luam loj.
Separation thiab Purification Workflow rau Fermentation Broth
Thaum fermentation tiav, centrifugation thiab pom yog thawj zaug ua kom tshem tawm cov kab mob ntawm tes khib nyiab thiab nruab nrab impurities, yielding ib tug ntshiab crude extract. Leveraging tus cwj pwm cationic ntawm polymyxin B, ion- pauv resin yog siv rau adsorptive enrichment; elution generates ib feem purified khoom tov.
Tom qab ultrafiltration thiab chromatographic sib cais tshem tawm cov kab mob heteropolypeptides, metabolic byproducts thiab pigments kom nce cov khoom purity.
Cov khoom siv tom qab ntawd acidified nrog sulfuric acid los hloov nws mus rau hauv polymyxin B sulfate, uas muaj cov tshuaj lom neeg zoo dua. Tsawg- qhov kub thiab txias concentration, lub tshuab nqus tsev ziab thiab milling yog ua raws li cov khoom siv hmoov kawg. Qhov kev ua kom huv huv no ua haujlwm zoo tshem tawm cov khoom siv sab nraud thiab ua kom cov kab mob muaj zog thiab purity ntawm cov khoom tiav ua raws li cov qauv tshuaj.
Kev hloov kho tshuaj thiab kev tsim kho zaum kawg
Ib txwm fermented polymyxin B muaj ntau yam isoforms xws li polymyxin B1 thiab B2. Kev hloov kho tshuaj me me yog siv los txhim kho kev ruaj ntseg thiab homogeneity ntawm cov khoom tiav los ntawm optimizing molecular spatial conformation, txo cov kab mob toxicity thaum tag nrho khaws cov tshuaj tua kab mob.
Cov khoom hloov kho tau ua kom tsis muaj menyuam tsis pom kev, aseptic ziab, pulverization thiab sieving kom tau txais txiaj ntsig zoo, zoo ib yam.polymyxin B hmoov. Tag nrho - txheej txheem sterile thiab impurity- tswj tuav tiv thaiv cov kab mob microbial, ruaj ntseg ntev - lub sij hawm cia ruaj khov thiab kev nyab xeeb ntawm cov hmoov formulation.
Cov hmoov ua tiav yog siv ncaj qha rau cov tshuaj pleev ib ce los tiv thaiv-kev kho kab mob, tshuaj tiv thaiv kab mob tiv thaiv kab mob, thiab kev sib xyaw ntawm kev npaj tshuaj kho mob.
Cov khoom lag luam, raws li cov tshuaj tua kab mob, muaj cov kev txhim kho tshwj xeeb hauv kev kho mob. Cov hauv qab no yog cov ncauj lus kom ntxaws tsom xam ntawm nws txoj kev loj hlob zeem muag:
Kev lag luam xav tau kev loj hlob
Nrog rau kev txhim kho ntawm cov qauv kev kho mob thoob ntiaj teb thiab kev siv zog ntawm cov pej xeem kev laus, qhov kev thov rau cov tshuaj tua kab mob txuas ntxiv mus ntxiv. Tshwj xeeb tshaj yog nyob rau hauv kev soj ntsuam nrog Gram negative bacterial infections, polymyxin B, raws li ib tug tseem ceeb chav kawm ntawv ntawm cov tshuaj tua kab mob, muaj tshwj xeeb antibacterial mechanisms thiab kho cov teebmeem, thiab yog li ntawd tau lug siv nyob rau hauv kev kho mob xyaum. Nyob rau hauv lub neej yav tom ntej, nrog rau kev xav tau ntau ntxiv rau kev tiv thaiv, tswj thiab kho cov kab mob sib kis, kev lag luam xav tau rau polymyxin B yuav tsum tau nce ntxiv.
Kev tshawb nrhiav thiab kev tsim kho tshiab tsav tsheb
Hauv kev tshawb fawb thiab kev tsim kho tshuaj, kev tsim kho tshiab yog ib qho tseem ceeb ntawm kev tsim cov tshuaj. Cov kws tshawb fawb tseem niaj hnub tshawb nrhiav cov chaw thov tshiab thiab txhim kho cov ntawv siv tshuaj rau polymyxin B txhawm rau txhim kho nws cov txiaj ntsig thiab kev nyab xeeb. Piv txwv li, los ntawm kev hloov txoj kev siv tshuaj, txhim kho cov hom phiaj tshuaj, thiab txo cov kev mob tshwm sim ntawm cov tshuaj, qhov kev siv tshuaj kho mob ntawm polymyxin B tuaj yeem txhim kho ntxiv. Cov R&D kev tsim kho tshiab no yuav coj nws txoj kev loj hlob dav dav.
FAQ
Dab tsi yog polymyxin B siv los kho?
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Polymyxin B yog ib qho tshuaj tua kab mob muaj zog uas siv los kho mob hnyav, nyuaj -kom- kho cov kab mob uas tshwm sim los ntawm Gram- cov kab mob tsis zoo. Vim nws muaj toxicity, kev siv lub cev feem ntau yog tshwj tseg rau tsev kho mob hnyav - kis tau tus kab mob uas tiv taus lwm yam tshuaj tua kab mob zoo dua.
Polymyxin B ua haujlwm sai npaum li cas?
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Polymyxin B ophthalmic tee (feem ntau ua ke nrog trimethoprim) yog cov tshuaj tua kab mob siv los kho cov kab mob qhov muag, nrog rau cov kab mob kab mob loj (qhov muag liab) thiab blepharoconjunctivitis.
Cim npe nrov: polymyxin b hmoov cas 1404-26-8, lwm tus neeg, manufacturers, hoobkas, lag luam wholesale, yuav, nqe, tej, kev muag khoom