Shaanxi BLOOM Tech Co., Ltd. yog ib qho ntawm cov tuam ntxhab thiab cov muag khoom ntawm fmoc-d-proline cas 101555-62-8 hauv Suav teb. Txais tos rau cov lag luam wholesale bulk zoo fmoc-d-proline cas 101555-62-8 muag ntawm no los ntawm peb lub hoobkas. Kev pabcuam zoo thiab tus nqi tsim nyog muaj.
Fmoc-D-prolineyog ib qho tseem ceeb uas tsis yog -natural amino acid derivative siv nyob rau hauv niaj hnub khoom -theem peptide synthesis. Nws cov qauv molecular ingeniously sib txuas cov cai tshwj xeeb -xa helical conformation ntawm D-proline nrog lub tswv yim tiv thaiv ntawm fluorene methoxycarbonyl: D-proline, raws li daim iav isomer ntawm proline, muaj lub inherent rigid pyrrolidine nplhaib uas muaj peev xwm induce lub reverse peptide kev sib raug zoo ntawm kev ruaj ntseg. molecules tiv thaiv protease degradation, thiab endowing nws nrog cov kev ua lom lom neeg txawv ntawm lub ntuj L- hom.
Thaum lub sab saum toj Fmoc tiv thaiv pab pawg, nrog rau nws zoo heev controllable acidity thiab alkalinity zog, yuav ua tau zoo tshem tawm nyob rau hauv me me alkaline tej yam kev mob, thiab nws muaj zog ultraviolet absorption vaj tse muab kev yooj yim rau tiag tiag - sij hawm soj ntsuam ntawm cov txheej txheem synthesis. Qhov kev sib xyaw ua ke no ua rau Fmoc-D-proline ib lub tsev tseem ceeb rau kev tsim cov tshiab- phiaj peptides, receptor antagonists, thiab conformationally txwv tsis pub ua, tshwj xeeb tshaj yog ua kom pom qhov tsis muaj txiaj ntsig hauv kev txhim kho cov tshiab chiral catalysts thiab cov ntaub ntawv ua haujlwm nrog cov khoom sib txawv ntawm qhov sib txawv, zoo heev {5} folding asymmetric synthesis thiab peptidopharmacology.
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Tshuaj Formula |
C20H19NO4 |
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Exact Mass |
337 |
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Molecular Luj |
337 |
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m/z |
337 (100.0%), 338 (21.6%), 339 (2.2%) |
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Elemental Analysis |
C, 71.20; H, 5.68; N, 4.15; O, 18.97 |
Hauv cov toj roob hauv pes loj ntawm niaj hnub biochemistry thiab kws tshuaj chemistry, Fmoc-D-proline zoo li lub hlaws ci ntsa iab, ci ntsa iab tsis tuaj yeem hloov pauv hauv ntau qhov kev txiav- ntug teb nrog nws cov qauv chiral tshwj xeeb thiab cov tshuaj ua haujlwm zoo heev. Cov hmoov dawb mus rau lub teeb daj crystalline hmoov no muaj qhov taw tes melting ntawm kwv yees li 102-116℃C thiab ib qho kev hloov kho qhov muag ntawm +33-33.5℃C (C=1, DMF). Nws yog yaj nyob rau hauv chloroform, dichloromethane, ethyl acetate DMSO, Acetone thiab lwm yam organic solvents, nrog lawv zoo meej ua ke ntawm Fmoc tiv thaiv pawg thiab D-configured proline, tau dhau los ua kub tseem ceeb tebchaw nyob rau hauv niaj hnub kev tshawb fawb thiab kev lag luam teb.
1. Qhov tseem ceeb nruab nrab ntawm cov tshuaj synthesis - "tus yuam sij" los ntawm cov tshuaj tua kab mob mus rau cov tshuaj tiv thaiv kab mob
Ua lub luag haujlwm tseem ceeb hauv kev lag luam tshuaj thiab tshuaj lom neeg engineering, nws yog ib qho tseem ceeb chiral intermediate rau synthesizing ntau yam bioactive molecules thiab tshuaj.
1.1. Tshuaj tua kab mob synthesis
D-proline nws tus kheej yog ib qho tseem ceeb structural unit nyob rau hauv lub molecular skeleton ntawm ntau yam tshuaj tua kab mob. Noj columnar crystal dawb pwm (guitarimycin) ua piv txwv, nws cov molecule muaj cov qauv proline derivative. Nws tuaj yeem siv los ua cov khoom pib rau kev sib txuas ntawm cov tshuaj tua kab mob tshiab macrolide thiab cov tshuaj tua kab mob semi hluavtaws.
Thiab tuaj yeem tsim cov tshuaj siv tshuaj molecular los ntawm kev hloov pauv hloov pauv ntawm nws pawg carboxyl thiab amino pawg.
2. Kev txhim kho ntawm cov tshuaj tiv thaiv -cov tshuaj qog
D-proline derivatives yog siv dav hauv kev txhim kho cov tshuaj tiv thaiv -cov qog nqaij hlav los tsim cov tebchaw nrog cov haujlwm lom neeg tshwj xeeb. Los ntawm asymmetric synthesis lub tswv yim, ntau cov tshuaj peptide thiab cov tshuaj molecule me me nrog kev tiv thaiv- qog nqaij hlav tuaj yeem npaj siv nws los ua ib qho chiral. Nws cov qauv pyrrolidine lub nplhaib tshwj xeeb tuaj yeem sim ua kom muaj kev sib haum xeeb hauv cov proteins thiab tsim kev sib raug zoo siab nrog cov hom phiaj protein.
3. Synthesis ntawm cov tshuaj tiv thaiv kab mob
Ib yam li ntawd, nws kuj yog ib qho tseem ceeb precursor rau synthesizing tshuaj antiviral. D-configured amino acids muaj qhov tshwj xeeb zoo nyob rau hauv antiviral peptide tsim - kab mob proteases muaj qis efficiency nyob rau hauv cleaving peptide daim ntawv cog lus muaj li ntawm D-amino acids, yog li endowing tshuaj muaj zog antiviral kev ua si thiab ntev ntawm kev ua.
4. Synthesis ntawm - carotene thiab retinal
Ntawm qhov kev ceeb toom tshwj xeeb yog D-proline tuaj yeem siv rau kev sib txuas ntawm - carotene thiab retinal. Retinal yog daim ntawv nquag ntawm vitamin A, ua lub luag haujlwm tseem ceeb hauv kev pom kev ua haujlwm, kev sib txawv ntawm tes, thiab kev tiv thaiv kab mob.
2. "lub hnub qub reagent" nyob rau hauv lub teb ntawm chiral chemistry - ib tug haib cuab tam rau kev sib cais cov neeg sawv cev thiab asymmetric synthesis
Nrog nws qhov chaw ntshiab chiral, nws muaj ntau yam kev siv hauv thaj chaw ntawm chiral chemistry.
2.1. Chiral cais tus neeg sawv cev
D-proline, tseem hu ua D-pyrrolidine-2-carboxylic acid, yog ib qho tseem ceeb chiral compound uas tuaj yeem siv los ua tus neeg sawv cev sib cais thiab chiral reagent. Nyob rau hauv tus txheej txheem ntawm kev sib cais racemic, tsis yog enantiomeric ntsev tuaj yeem tsim nrog lub hom phiaj racemic amine lossis acid.
Thiab lawv qhov sib txawv solubility tuaj yeem siv los ua kom muaj kev sib cais zoo. Txoj kev no yooj yim rau kev khiav lag luam thiab tus nqi - ua tau zoo, ua rau nws yog ib lub tswv yim zoo rau kev lag luam qib chiral sib cais.
2.2. Asymmetric catalysis
Nyob rau hauv lub tshav pob ntawm asymmetric synthesis, proline thiab nws cov derivatives yog ib qho ntawm feem ntau classic organic me me molecule catalysts. Khaws lub catalytic active centre ntawm proline - nws cov qauv amine theem nrab tuaj yeem qhib cov carbonyl tebchaw los ntawm enamine intermediates, ua tiav siab enantioselectivity hauv Aldol cov tshuaj tiv thaiv, Mannich cov tshuaj tiv thaiv, Michael ntxiv, thiab lwm yam.
Lub npe nrov Hajos Paris Der Sauer Wiechert cov tshuaj tiv thaiv thiab Sau npe Barbas CBS txo cov tshuaj tiv thaiv, ob qho tib si catalyzed los ntawm proline, nthuav tawm qhov tsis txaus ntseeg tsis txaus ntseeg. Fmoc-D-proline ntxiv ib pab pawg tiv thaiv Fmoc ntawm lub hauv paus no, ua rau nws ua haujlwm yooj yim dua hauv ntau- kauj ruam synthesis.
2.3. Chiral tshuaj intermediates
Los ntawm synthesizing asymmetric D-proline, cov tebchaw nrog cov haujlwm lom neeg tshwj xeeb tuaj yeem npaj rau kev tshawb fawb tshuaj thiab kev loj hlob. Nrog rau kev sib txuas ntxiv ntawm kev tshawb fawb thiab kev loj hlob ntawm cov tshuaj, nws yuav tsum tau siv rau hauv kev npaj cov tshuaj chiral tshiab lossis kev txhim kho cov txheej txheem tshuaj synthesis uas twb muaj lawm.
3. Ntau yam hauv Organic Synthesis - Los ntawm Catalysts mus rau Flavoring Agents
3.1. Organic tshuaj tiv thaiv catalyst
Proline thiab nws cov derivatives feem ntau ua raws li asymmetric catalysts hauv cov tshuaj tiv thaiv organic. Cov tshuaj tiv thaiv catalytic aldol condensation ntawm proline hauv CBS txo cov tshuaj tiv thaiv yog qhov piv txwv tseem ceeb tshaj plaws. Nyob rau hauv lub hauv paus no, nrog rau kev qhia ntawm Fmoc pawg, nws solubility thiab cov tshuaj tiv thaiv selectivity yog ntxiv optimized, thiab nws yuav siv tau raws li ib tug npaum catalyst nyob rau hauv hydrogenation, polymerization, hydrolysis thiab lwm yam tshuaj, nrog rau cov yam ntxwv zoo xws li muaj zog kev ua si thiab zoo stereospecificity.
3.2. Flavoring agents thiab zaub mov kev lag luam
Ib me ntsis -paub tab sis kev lag luam muaj txiaj ntsig zoo siv yog tias thaum rhuab nrog cov suab thaj, cov tshuaj tiv thaiv amino carbonyl (Maillard cov tshuaj tiv thaiv) tuaj yeem tshwm sim, tsim cov tshuaj nrog ib qho tshwj xeeb aroma. Cov yam ntxwv no ua rau nws tsim nyog rau kev siv los ua ib qho tsw enhancer hauv kev lag luam khoom noj. Tsis tas li ntawd, thaum lub sij hawm brewing txheej txheem, cov proteins uas nplua nuj nyob rau hauv proline thiab khi rau polyphenols tuaj yeem tsim tshwj xeeb haze (turbidity), uas cuam tshuam rau lub siab zoo ntawm cov cawv.
3. Cov ntaub ntawv nyoos rau kev sib txuas ntawm cov roj cholesterol ester inhibitors
Nws tseem yog cov khoom siv hluavtaws rau cov roj cholesterol ester inhibitors, uas muaj peev xwm siv tau tus nqi hauv kev txhim kho cov kab mob plawv-txog tshuaj.
4. Kev Tshawb Fawb Txog Kev Noj Qab Haus Huv thiab Kev Siv Hluav Taws Xob - Los ntawm Kev Cuam Tshuam Txog Kev Nyuaj Siab
Amino acids thiab amino acid derivatives tau ua lag luam ua lub zog ntxiv. Kev tshawb fawb tau qhia (Luckose F, et al., Crit Rev Food Sci Nutr. 2015; 55 (13): 1793-1814) tias lawv tuaj yeem:
cuam tshuam rau kev tso tawm ntawm cov tshuaj hluavtaws metabolic: txhawb kev tso tawm ntawm cov tshuaj hormones loj hlob thiab insulin-zoo li kev loj hlob, uas muaj txiaj ntsig zoo rau cov leeg nqaij protein synthesis.
Ua kom cov roj tau zoo thaum lub sijhawm ua haujlwm: Thaum lub sijhawm ua haujlwm siab - siv zog, proline tuaj yeem ua lwm yam khoom siv hluav taws xob.
Muab electrons rau lub ubiquinone reservoir los ntawm PRODH (proline dehydrogenase) txoj kev, txhawb oxidative phosphorylation los tsim ATP.
Txhim kho kev puas siab puas ntsws: Thaum muaj kev ntxhov siab- cov haujlwm muaj feem xyuam, cov amino acid derivatives tuaj yeem txhim kho kev txawj ntse thiab kev saib xyuas.
Tiv thaiv cov nqaij ntshiv los ntawm kev tawm dag zog: Proline muaj peev xwm txo qis oxidative kev nyuaj siab, nce superoxide dismutase (SOD) kev ua, txo cov qib malondialdehyde, thiab tiv thaiv cov leeg hlwb los ntawm kev tawm tsam dawb radicals.
Hauv kev yug tsiaj, kev noj zaub mov thiab kev ua haujlwm ntawm lub cev ntawm proline kuj muaj kev txhawj xeeb heev. Kev tshawb fawb tau pom tias proline tuaj yeem txo cov kev ntxhov siab oxidative hauv lub cev, koom nrog hauv lub zog metabolism, tswj kev tiv thaiv kab mob, txhawb kev tsim cov protein, thiab txhim kho tsiaj kev loj hlob thiab kev ua me nyuam. Ntxiv qhov tsim nyog ntawm proline rau kev noj zaub mov hauv npua, qaib, thiab aquaculture tuaj yeem txhim kho lub peev xwm antioxidant thiab kev loj hlob ntawm cov tsiaj. Txawm hais tias feem ntau yog siv rau kev tshawb fawb txog kev tshawb fawb, nws niam nws txiv D-proline muaj cov ntawv thov dav dav hauv cov khoom noj tsiaj.
5. Enamel Restoration thiab Biomaterials Research: Frontier Exploration
Raws li kev tshawb fawb tshiab los ntawm University of Illinois, qhov rov ua ntu zus ntawm proline plays lub luag haujlwm tseem ceeb hauv cov enamel proteins. Qhov ntev ntawm cov proline rov ua ntu yog, qhov loj dua cov protein ua npuas ncauj, qhov zoo tshaj plaws ntawm cov enamel prism qauv yog, thiab cov hniav muaj zog thiab elastic dua. Qhov kev tshawb pom no muab lub hauv paus theoretical rau kev tsim cov hniav cuav tshiab, thiab raws li kev tiv thaiv derivative ntawm proline, nws muaj nuj nqis tseem ceeb hauv kev tshawb fawb synthesis ntawm cov khoom siv biomaterials.
Tsis tas li ntawd, proline kuj tseem ua lub luag haujlwm tseem ceeb hauv kev kawm txog cov qauv thiab kev ua haujlwm ntawm cov proteins xws li tshuaj tiv thaiv kab mob, amyloid proteins, thiab prion proteins. Muab siab -cov ntaub ntawv tshawb fawb purity rau cov kev txiav - cov ncauj lus.
Fmoc-D-proline tsis yog ib qho tshuaj reagent zoo tib yam, tab sis yog ib qho kev sib txuas lus tseem ceeb uas nthuav dav ntau yam xws li peptide synthesis, kev tsim tshuaj, chiral chemistry, organic catalysis, kev tshawb fawb txog zaub mov, thiab biomaterials. Los ntawm milligram qib peptide synthesis nyob rau hauv lub chav kuaj mus rau ton theem tshuaj intermediate ntau lawm nyob rau hauv industrial rhiav; Los ntawm kev siv zog ntxiv rau hauv kev ua kis las mus rau kev tshawb fawb txog kev kho hniav hauv cov chaw kho hniav, F txuas ntxiv mus thawb cov ciam teb ntawm cov chemistry niaj hnub thiab kev tshawb fawb txog kev ua neej sab nraud nrog nws cov kev sib txawv tsis sib xws.
Nyob rau hauv lub xub ntiag ntawm alkali, nws yog tau los ntawm reacting 9-fluorenylmethoxycarbonyl chloride nrog D-proline. Txoj kev no yog yooj yim rau kev khiav lag luam, me me nyob rau hauv cov tshuaj tiv thaiv tej yam kev mob, siab nyob rau hauv yield, thiab dav siv rau kev npaj ntawm cov khoom. Lub synthesis tshuaj sib npaug ntawmFMOC-D-prolineyog qhia hauv daim duab hauv qab no:
Txoj Kev 1:
Dissolve D-proline hauv 115ml ntawm 10% sodium carbonate aqueous tov.
Cov kauj ruam no yog cov txheej txheem kev sib cais ntawm lub cev, tsis muaj tshuaj sib npaug.
Txias cov tshuaj yaj mus rau -4-0 degree.
Cov kauj ruam no yog txheej txheem cua txias ntawm lub cev, thiab tseem tsis muaj tshuaj sib npaug.
Dissolve 9-fluorenylmethoxycarbonyl chloride nyob rau hauv 35.0ml ntawm acetone thiab ntxiv dropwise rau lub txias system.
Cov kauj ruam no kuj yog ib qho kev sib cais ntawm lub cev thiab cov txheej txheem dropwise ntxiv, tsis muaj tshuaj sib npaug. Tab sis cov tshuaj tiv thaiv tom ntej yog ib kauj ruam tseem ceeb.
Tom qab qhov sib ntxiv ntawm dropwise tiav, do hauv dej khov rau 30 feeb, thiab tom qab ntawd do hauv chav sov li 2.0 teev.
Thaum lub sijhawm txheej txheem no, 9-fluorenylmethoxycarbonyl chloride raug acylation cov tshuaj tiv thaiv nrog cov amino pawg D-proline los tsim. Tab sis cov kev sib npaug tshuaj tshwj xeeb tau muab rau hauv cov lus piav qhia dhau los thiab yuav tsis rov hais dua ntawm no.
Saib xyuas qhov kev nce qib ntawm cov tshuaj tiv thaiv siv nyias - txheej chromatography.
Cov kauj ruam no yog cov txheej txheem analytical, tsis muaj tshuaj sib npaug.
Tom qab cov tshuaj tiv thaiv tiav, ncuav cov sib tov rau hauv 400ml dej.
Cov kauj ruam no yog txheej txheem ntawm lub cev dilution, tsis muaj tshuaj sib npaug.
Extract ob zaug nrog ether.
Cov kauj ruam no yog cov txheej txheem rho tawm lub cev siv los rho tawm cov khoom, tsis muaj tshuaj sib npaug.
Txias thiab kho cov pH mus rau ib ncig ntawm 2 nrog cov concentrated hydrochloric acid, uas ua rau cov nag lossis daus loj ntawm cov khoom dawb.
Cov kauj ruam no suav nrog kev kho cov pH thiab precipitating cov khoom.
Txawm hais tias qhov kev sib npaug tshuaj tshwj xeeb nyuaj rau sau, nws tuaj yeem nkag siab tiasFmoc-D-prolineprecipitates los ntawm kev daws nyob rau hauv acidic tej yam kev mob.
Extract peb zaug nrog 50ml ntawm ethyl acetate.
Cov kauj ruam no yog txheej txheem rho tawm lub cev siv los ntxiv cov khoom rho tawm, tsis muaj tshuaj sib npaug.
Qhuav nrog anhydrous magnesium sulfate.
Cov kauj ruam no yog txheej txheem ziab lub cev siv los tshem tawm cov dej noo, tsis muaj tshuaj sib npaug. Tab sis magnesium sulfate yuav nqus dej noo los tsim magnesium sulfate hydrated, raws li tau hais dhau los.
Tshem tawm cov kuab tshuaj los ntawm lub tshuab nqus tsev distillation.
Cov kauj ruam no yog txheej txheem ntawm lub cev distillation siv los tshem tawm cov kuab tshuaj, tsis muaj tshuaj sib npaug.
Siv petroleum ether los ua cov khoom lag luam.
Cov kauj ruam no yog cov txheej txheem nag lossis daus ntawm lub cev, uas suav nrog kev hloov pauv cov kuab tshuaj kom ua rau cov khoom tsis muaj tshuaj sib npaug.
Nyob rau hauv cov ntsiab lus, cov tshuaj synthesis cov kauj ruam muaj xws li dissolution, txias, dropwise ntxiv, stirring cov tshuaj tiv thaiv, soj ntsuam kev kawm, post-kev kho mob (dilution, extraction, pH adjustment, nag lossis daus), extraction thiab purification (extraction, drying, distillation, nag lossis daus). Cov tshuaj tiv thaiv tseem ceeb hauv cov txheej txheem no yog cov tshuaj tiv thaiv acylation ntawm 9-fluorenylmethoxycarbonyl chloride thiab D-proline. Lwm cov kauj ruam tseem ceeb yog kev ua haujlwm ntawm lub cev, uas yog qhov tseem ceeb rau kev ua kom huv thiab sau cov khoom. Los ntawm cov kauj ruam no, nws tuaj yeem npaj tau.
Cim npe nrov: fmoc-d-proline cas 101555-62-8, lwm tus neeg, manufacturers, hoobkas, lag luam wholesale, yuav, nqe, tej, kev muag khoom