Shaanxi BLOOM Tech Co., Ltd. yog ib qho ntawm cov tuam ntxhab thiab cov muag khoom ntawm beta-neoendorphin hauv Suav teb. Txais tos rau cov lag luam wholesale bulk zoo beta-neoendorphin muag ntawm no los ntawm peb lub hoobkas. Kev pabcuam zoo thiab tus nqi tsim nyog muaj.
Beta-neoendorphinyog ib qho endogenous opioid peptide teej tug mus rau tsev neeg Dynorphin. Nws tau pib cais thiab txheeb xyuas los ntawm hypothalamus ntawm npua los ntawm Japanese kws tshawb fawb Matsuo li al. hauv xyoo 1980s. Nws yog oligopeptide muaj li ntawm 9 amino acid residues. Nws ua tiav ib ntus yog Tyr Gly Gly Phe Leu Arg Lys Tyr Pro. Lwm peptide ze ze rau nws yog alpha neoendorphin, uas muaj 10 amino acids (nrog ib qho ntxiv Lys residue tom kawg). Ob leeg pib los ntawm tib lub precursor protein.
beta-Neoendorphin COA
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| Certificate of Analysis | ||
| Compound npe | Beta{0}}Neoendorphin | |
| Qib | Pharmaceutical qib | |
| CAS Nr. | 77739-21-0 | |
| Ntau | 33g | |
| Ntim txheem | PE hnab + Al foil hnab | |
| Chaw tsim tshuaj paus | Shaanxi BLOOM TECH Co., Ltd | |
| Ntau No. | 202601090088 | |
| MFG | Peb 9, 2026 | |
| EXP | Peb 8, 2029 | |
| Qauv |
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| Yam khoom | Enterprise txheem | Kev txheeb xyuas qhov tshwm sim |
| Qhov tshwm sim | Dawb los yog yuav luag dawb hmoov | Ua raws |
| Cov ntsiab lus dej | Tsawg dua lossis sib npaug li 5.0% | 0.26% |
| Poob rau ziab | Tsawg dua lossis sib npaug li 1.0% | 0.77% |
| Hnyav Hlau | Pb Tsawg dua lossis sib npaug li 0.5ppm | N.D. |
| Tsawg dua lossis sib npaug li 0.5ppm | N.D. | |
| Hg Tsawg dua lossis sib npaug li 0.5ppm | N.D. | |
| Cd Tsawg dua lossis sib npaug li 0.5ppm | N.D. | |
| Purity (HPLC) | Ntau dua lossis sib npaug li 99.0% | 99.80% |
| Ib qho impurity | <0.8% | 0.32% |
| Tag nrho microbial suav | Tsawg dua lossis sib npaug li 750cfu / g | 337 |
| E. Coli | Tsawg dua lossis sib npaug li 2MPN / g | N.D. |
| Salmonella | N.D. | N.D. |
| Ethanol (los ntawm GC) | Tsawg dua lossis sib npaug li 5000ppm | 5 56 ppm |
| Cia | Khaws rau hauv qhov chaw kaw, tsaus, thiab qhuav hauv qab -20 degree | |
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| Chemical Formula: | C54H77N13O12 |
| Exact Mass: | 1100 |
| Molecular Luj: | 1100 |
| m/z: | 1100 (100.0%), 1101 (58.4%), 1102 (16.7%), 1101 (4.8%), 1103 (3.1%), 1102 (2.8%), 1102 (2.5%), 1103 (1.4%) |
| Elemental Analysis: | C, 58.95; H, 7.05; N, 16.55; O, 17.45 |
Raws li cov khoom tseem ceeb yog muaj nyob rau hauv endogenous neuropeptide, nws "siv" tsis tsuas yog npog nws lub ntuj physiological functions nyob rau hauv tib neeg lub cev, tab sis kuj muaj xws li kev tshawb fawb ntawm nws siv raws li ib tug tshuaj hom phiaj nyob rau hauv kev tshawb fawb kev tshawb fawb, raws li zoo raws li nws daim ntawv thov nyob rau hauv lub teb ntawm cosmetic chemistry nyob rau hauv xyoo tas los.
Biomedical tshawb fawb daim ntaub ntawv: teeb liab transduction thiab receptor tshawb fawb
Nyob rau hauv molecular biology thiab neuroscience laboratories, nws yog ib tug txheej txheem tshuaj cuab yeej rau kev kawm opioid receptor signaling.
1.1 Selective agonists ntawm kappa opioid receptor (KOR)
Cov kws tshawb fawb siv cov khoom sib xyawbeta-neoendorphinlos kawm txog kev ua haujlwm ntawm KOR. Tsis zoo li cov tshuaj opioid hluavtaws, beta endorphins sawv cev rau hom kev ua kom lub cev ntawm lub cev.
Kev siv piav qhia: Siv rau hauv vitro cell thwmsim los txiav txim siab receptor affinity (Ki), agonist potency (EC50), thiab G protein coupling efficiency. Los ntawm kev sib piv cov txiaj ntsig ntawm beta endorphin thiab dynorphin A, cov kws tshawb fawb tuaj yeem txiav txim siab qhov sib txawv me me hauv KOR subtypes.
Kev tshawb fawb tus nqi: Pab nkag siab tias yog vim li cas ntuj ligands qhib receptors thiab tsis tshua muaj peev xwm tsim kev mob siab ntev zoo li cov tshuaj cuav.
1.2 Kawm txog Neural Loop Tracing thiab Coexistence ntawm Neurotransmitters
Vim nws qhov kev qhia siab nyob rau hauv hypothalamus thiab pituitary caj pas, nws yog siv los ua ib qho immunohistochemical (IHC) marker los txheeb xyuas thiab taug qab cov kab mob neural tshwj xeeb.
Lub hom phiaj piav qhia: Txhawm rau kawm txog kev tswj hwm dopamine hauv txoj hauv kev substantia nigra striatum thiab kev sib koom ua ke ntawm opioid peptides thiab vasopressin hauv hypothalamic supraoptic nucleus.
Physiological Function Application: Natural regulator nyob rau hauv tib neeg lub cev
Hauv tib neeg lub cev, nws tsis yog 'tshuaj txawv teb chaws', tab sis yog ib qho tseem ceeb molecule uas ua haujlwm rau ntau lub hom phiaj ntawm lub cev.
2.1 Endogenous analgesia (kev tswj mob)
Qhov no yog nws qhov tseem ceeb physiological siv. Thaum lub cev raug kev puas tsuaj rau lub cev, lub hauv paus paj hlwb tso tawm peptide, uas ua rau cov leeg txha caj qaum thiab hlwb.
Cov lus piav qhia ntxaws: Nws thaiv kev tso tawm ntawm cov tshuaj P thiab glutamate los ntawm presynaptic inhibition, yog li txo qhov mob ntawm qhov mob.
Hauv cov qauv mob ntev, nce nws cov concentration hauv cerebrospinal kua tuaj yeem ua rau muaj qhov mob hnyav.
2.2 Kev ntxhov siab thiab kev xav ruaj khov
Beta endorphins ua si lub luag haujlwm "brake" hauv kev ntxhov siab.
Cov lus piav qhia ntxaws: Hauv kev ntxhov siab, lub hypothalamus tso tawm peptide no los tswj HPA axis (hypothalamic pituitary adrenal axis). Nws tuaj yeem tiv thaiv kev tso tawm ntau dhau ntawm cov tshuaj hormones kev ntxhov siab thiab tiv thaiv kev puas tsuaj neuronal los ntawm kev ntxhov siab ntev ntev hauv lub cev.
Txoj cai thim rov qab: Nws kuj koom nrog hauv kev tsim "dysphoria", zoo li qhov ua tsis zoo uas yog qhov kev tiv thaiv tsis zoo los ntawm lub cev kom tsis txhob muaj ntau dhau ntawm kev coj tus cwj pwm zoo.
2.3 Neuroendocrine tswj
Kev tswj ntawm lub cev xeeb tub: Nws tuaj yeem cuam tshuam lub pulsatile secretion ntawm gonadotropin- tso cov tshuaj hormones (GnRH), yog li tswj ovulation thiab fertility muaj nuj nqi nyob rau hauv kev ntxhov siab heev los yog malnutrition. Dej ntsev tshuav: ua hauj lwm nrog antidiuretic hormone los tswj cov reabsorption ntawm dej los ntawm lub raum.
Muaj peev xwm siv tshuaj kho mob thiab kho mob
Txawm hais tias beta endorphins tseem tsis tau ua lag luam raws li thawj zaug - cov tshuaj siv tshuaj, lawv cov kev tshawb fawb soj ntsuam kev txhais lus tseem ceeb heev.
3.1 Kev txhim kho ntawm cov tshuaj tsis muaj yees tshiab
Traditional mu receptor agonists xws li morphine thiab fentanyl yog qhov muaj yees heev.Beta-neoendorphinzoo li yuav qhib kappa receptors.
Cov lus piav qhia siv: Pharmacologists sim ua cov qauv ntawm beta endorphins thiab tsim "Biased Ligands". Cov tshuaj no tsom mus khaws nws cov nyhuv analgesic thaum tsis txhob muaj kev phiv los ntawm kev hnov lus thiab chim siab los ntawm txoj kev tshwj xeeb receptor ua ke.
3.2 Kev cuam tshuam rau Kev Siv Tshuaj
Cov lus piav qhia ntxaws: Hauv kev kho cov yeeb tshuaj yeeb lossis haus cawv, lub kaw lus yog siv los txo cov dopamine surges hauv limbic system. Kev tshawb fawb tau pom tias txhim kho cov qib endorphins tshiab los ntawm kev siv tshuaj kho mob tuaj yeem txo cov tshuaj cravings thaum tshem tawm.
3.3 Antiepileptic nyhuv
Cov lus piav qhia ntxaws: Cov ntaub ntawv pov thawj pom tau hais tias tom qab mob qaug dab peg loj, qib ntawm beta endorphin hauv lub hlwb nce. Cov kev tshawb fawb soj ntsuam tau tshawb fawb txog kev siv qhov ntswg (kev hla cov ntshav- lub paj hlwb barrier) raws li ib qho kev pabcuam txiav tawm rau kev mob qaug dab peg, siv nws cov teebmeem neuroprotective muaj zog.
Dermatology thiab Cosmetic Chemistry Applications
Qhov no yog daim ntawv thov ze tshaj plaws ntawm beta endorphins rau cov neeg siv khoom lag luam hauv xyoo tas los no, tshwj xeeb tshaj yog nyob rau hauv siab -kawg ua haujlwm ntawm cov khoom siv tawv nqaij.4.1 Kev kho tawv nqaij tawv thiab tiv thaiv - cov teebmeem inflammatory
Cov lus piav qhia ntxaws: Cov tawv nqaij epidermal hlwb (keratinocytes) qhia opioid receptors. Beta endorphins tuaj yeem txhawb nqa kev sib txuas ntawm ceramides thiab ntxiv dag zog rau daim tawv nqaij cov qauv phab ntsa.
Nyob rau tib lub sijhawm, nws tuaj yeem inhibit degranulation ntawm mast hlwb thiab txo cov kev mob tshwm sim xws li tawv nqaij liab, o, thiab khaus.
4.2 Bioactive tshuaj ntawm lub tswvyim ntawm "kev xav ntawm daim tawv nqaij"
Cov lus piav qhia ntxaws: Qee cov khoom xyaw ntawm cov khoom siv tawv nqaij, xws li cov tau los ntawm grey taum extract lossis hluavtaws peptides, thov kom muaj peev xwm txhawb nqa cov tawv nqaij ntawm tus kheej tsim ntawm beta endorphins, ua tiav "kev ntxhov siab" thiab "kev nyem tam sim". Qhov kev siv no siv cov yam ntxwv ntawm daim tawv nqaij thiab lub paj hlwb sib koom ua ke ntawm cov teeb liab molecules (cov tawv nqaij hlwb axis).
Hauv cov kab mob muaj sia, cov peptide no tsis yog tsim los ntawm tib neeg txhais lus, tab sis yog ib feem ntawm cov protein ntau dua, tsim los ntawm txoj hauv kev "precursor processing".
Biosynthetic keeb kwm ntawm beta endorphins nyob rau hauv PDYN noob nyob rau hauv lub nucleus ntawm lub cell (ntawm chromosome 20 nyob rau hauv tib neeg).
Transcription thiab Txhais:
Hauv cov neurons lossis endocrine hlwb, PDYN noob tau hloov mus rau hauv mRNA, uas tom qab ntawd tau muab txhais ua prepro dynorphin ntawm ribosomes ntawm cov tawv nqaij endoplasmic reticulum (RER).
Teeb liab peptide resection:
Thawj cov protein muaj N-terminal signal peptide lub luag haujlwm coj nws mus rau txoj kev zais cia. Thaum nyob rau hauv lub endoplasmic reticulum lumen, teeb liab peptides yog cleaved los ntawm lub teeb liab peptidases los tsim prodynorphin.
Tom qab ua tiav thawj zaug folding hauv endoplasmic reticulum, proenkephalin raug thauj mus rau Golgi apparatus ntawm vesicles.
Ntim:
Hauv qhov rov qab network (TGN) ntawm Golgi apparatus, proenkephalin tau ntim nrog cov txheej txheem tshwj xeeb rau hauv cov granule ntom ntom vesicles (LDCVs).
Kev loj hlob:
Qhov tseeb biosynthetic cleavage txheej txheem feem ntau tshwm sim thaum lub sij hawm thauj cov vesicles los ntawm Golgi apparatus mus rau synaptic terminals. Raws li tus nqi pH hauv cov hlab ntsha txo qis (acidification), cov enzymes ua haujlwm tau qhib.
Qhov no yog qhov tseem ceeb tshaj plaws biochemical kauj ruam hauv cov txheej txheem synthesis. Proenkephalin yog cov saw loj polypeptide uas muaj ntau yam opioid peptide sib lawv liag (xws li enkephalin A, enkephalin B, thiab neoenkephalin).
Lub luag haujlwm ntawm prohormone converting enzyme (PC)
Lub synthesis ntawm beta endorphins tsuas yog nyob ntawm ob lub endonucleases, PC1/3 thiab PC2.
Kev txheeb xyuas qhov chaw:
Cov enzymes no paub txog ob qhov yooj yim amino acid qhov chaw nyob rau hauv ib ntus (xws li Lys Arg lossis Arg Arg).
Tsim alpha tshiab endorphins:
Lub enzyme ua ntej cleaves lub precursor, tso alpha tshiab endorphins. Alpha hom yog 10 peptide nrog cov kab ke Tyr Gly Gly Phe Leu Arg Lys Tyr Pro Lys.
Kev hloov kho zoo ntawm Carboxypeptidase E (CPE)
Txhawm rau hloov los ntawm hom alpha mus rau hom beta, ntxiv cleavage ntawm C-terminal residue yuav tsum tau.
Kauj ruam:
Carboxypeptidase E paub txog alkaline amino acid (Lys) tom kawg ntawm alpha endorphins.
Hloov dua siab tshiab:
CPE tshem tawm 10th Lys thaum kawg los tsim 9-peptide beta neoendorphin (Tyr Gly Gly Phe Leu Arg Lys Tyr Pro).
Cov synthesis tus nqi thiab qhov kawg tawm los yog cuam tshuam los ntawm ntau yam ib puag ncig cov cim:
Calcium ion signal:
Lub depolarization ntawm cov neurons tuaj yeem ua rau muaj kev nce ntxiv hauv cov calcium hauv cov cell, uas tsis yog tsuas yog txhawb nqa vesicle tso tawm, tab sis kuj txhawb kev hloov pauv ntawm PDYN noob.
cAMP cov ntsiab lus teb:
Lub cheeb tsam txhawb nqa ntawm PDYN noob muaj cAMP cov ntsiab lus teb (CRE). Thaum cov hlwb tau txais cov cim kev ntxhov siab (xws li dhau los ntawm norepinephrine receptors), cAMP qib nce, uas ua rau cov synthesis ntawm peptide.
Cov ntaub so ntswg tshwj xeeb:
Txawm hais tias cov precursor proteins yog tib yam, nyob rau hauv cov ntaub so ntswg sib txawv (xws li thalamus vs. spinal cord), vim hais tias qhov sib txawv qhia piv ntawm kev ua enzymes (PC1 vs. PC2), qhov feem ntawm alpha hom thiab beta hom nyob rau hauv cov khoom kawg yuav txawv.
Ntxiv nrog rau kev sib xyaw ua ke ntawm ntuj tsim, biotechnology niaj hnub no kuj tau tsim cov txheej txheem hluavtaws, feem ntau yog siv rau kev tshawb fawb tshawb fawb thiab cov khoom siv raw:
Genetic Engineering Recombination Method
Siv Escherichia coli (E. coli) los yog poov xab ua tus tswv tsev.
Txoj kev:
Artificially synthesizedbeta-neoendorphinDNA ib ntus yog fused thiab qhia nrog cov cab kuj protein (xws li GST) los tiv thaiv cov peptide me me los ntawm kev degraded los ntawm tus tswv tsev proteases.
Purification:
Tom qab kev qhia, cov ntshiab peptide yog sib cais los ntawm affinity chromatography thiab ces cleaved thiab tso tawm siv tshuaj reagents (xws li cyanogen bromide) los yog tej enzymes.
Natural biosynthesis yog cov txheej txheem sib xyaw ua ke uas pib nrog kev hloov pauv ntawm PDYN noob, thauj los ntawm endoplasmic reticulum Golgi system, thiab thaum kawg ua tiav los ntawm cascade cleavage ntawm PC thiab CPE enzymes hauv secretory vesicles.
Cov ntaub ntawv Source Statement:
'Nws yog qhov txaus los ua haujlwm nrog Creative. amazingy koom, yooj yim rau kev sib txuas lus nrog. teb nrog iterations tom ntej, thiab ua haujlwm zoo nkauj.
Molecular Biology ntawm Cell (Alberts li al.):
Cov hauv paus ntsiab lus ntawm cov protein secretion pathways thiab precursor ua.
Opioid Receptors (Pasternak, GW):
Qhia ntxaws txog qhov chaw enzymatic hydrolysis ntawm txhua tus tswv cuab ntawm tsev neeg proenkephalin.
Phau ntawv Journal of Biological Chemistry (JBC):
Ib daim ntawv tshawb fawb txog lub luag haujlwm tshwj xeeb ntawm PC1 thiab PC2 enzymes hauv opioid peptide synthesis.
IUPHAR Database:
Kev piav qhia ntawm cov txheej txheem endogenous ligand biosynthesis.
ntawv pov thawj:
1. Matsuo, H. & Kangawa, K. (1982). "beta-Neo-endorphin: cov qauv thiab kev ua haujlwm." Kev tshuaj xyuas txhua xyoo ntawm Physiology.
2. Civelli, O., et al. (1985). "Molecular biology ntawm opioid peptide precursors." Kev tshuaj xyuas txhua xyoo ntawm Neuroscience.
3. Zadina, JE, et al. (1997). "Ib tug muaj zog thiab xaiv endogenous agonist rau mu-opioid receptor." Xwm.
4. Basbaum, AI, et al. (2009). "Cellular thiab Genetic Mechanisms of Mob." Cell.
5. Takahashi, M., et al. (2018). "Lub luag haujlwm ntawm beta-neoendorphin hauv daim tawv nqaij barrier muaj nuj nqi." Phau ntawv Journal of Investigative Dermatology.
6. IUPHAR/BPS Phau Ntawv Qhia Txog Tshuaj Kho Mob.
Cim npe nrov: beta-neoendorphin, lwm tus neeg, manufacturers, hoobkas, lag luam wholesale, yuav, nqe, tej, kev muag khoom