C Peptideyog peptide compound nrog cov amino acid ib ntus. Nws cov qauv molecular ruaj khov, tso cai rau C-peptide tswj lub sijhawm ua haujlwm ntev hauv lub cev. Tsis tas li ntawd, qhov hnyav molecular ntawm C-peptide yog nruab nrab, thiab nws tsis yooj yim tshem tawm los ntawm ob lub raum vim nws qhov me me, thiab nws tsis cuam tshuam rau nws cov diffusion hauv cov ntaub so ntswg vim nws loj. Feem ntau, kuv muaj cov hmoov dawb nrog cov mis molecular C137H225N37O49 thiab CAS 39016-05-2. Nws yog ib hom kab mob pancreatic islet Peptides secreted los ntawm cov cell coexist nrog insulin nyob rau hauv sib npaug molecular tooj nyob rau hauv lub proinsulin molecule thiab tso tawm mus rau hauv cov hlab ntsha ua ke. C-peptide tsis yog metabolized los ntawm daim siab thiab muaj ib nrab-lub neej ntev dua li insulin, yog li ntsuas C-peptide tuaj yeem cuam tshuam zoo dua Cell synthesis thiab insulin tso tawm ua haujlwm. Lub cev lub cev ntawm C-peptide yog qhov tseem ceeb heev rau nws cov kev siv hauv kev lag luam biology thiab tshuaj. Zoo biocompatibility, tsis muaj toxicity lossis kev phiv rau tib neeg lub cev. Qhov no ua rau C-peptide muaj kev nyab xeeb thiab muaj txiaj ntsig zoo rau kev kuaj mob thiab kev kho mob hauv cov tshuaj kho mob. Piv txwv li, cov islets ntawm cov neeg mob ntshav qab zib tuaj yeem ntsuas tau los ntawm kev ntsuas qib ntawm cov ntshav C-peptide Cov haujlwm ua haujlwm ntawm cov hlwb; Nyob rau tib lub sijhawm, C-peptide tuaj yeem siv los npaj cov tshuaj npaj hauv kev kho tshuaj insulin hloov kho kom txhim kho cov ntshav qab zib tswj cov neeg mob ntshav qab zib.
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Customized Bottle Caps Thiab Corks:
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Tshuaj Formula |
C137H225N37O49 |
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Exact Mass |
3173 |
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Molecular Luj |
3175 |
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m/z |
3174 (100.0%), 3175 (73.5%), 3173 (67.5%), 3176 (24.7%), 3176 (11.1%), 3177 (11.1%), 3176 (10.1%), 3175 (9.2%), 3174 (9.2%), 3175 (6.8%), 3176 (5.9%), 3177 (4.5%), 3175 (4.4%), 3176 (4.1%), 3177 (3.9%), 3178 (3.5%), 3177 (2.9%), 3175 (2.6%), 3178 (2.5%), 3177 (1.9%), 3175 (1.9%), 3174 (1.7%), 3178 (1.3%), 3174 (1.3%), 3176 (1.2%), 3178 (1.1%), 3179 (1.1%), 3177 (1.0%) |
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Elemental Analysis |
C, 51.83; H, 7.14; N, 16.33; O, 24.70 |
C Peptide, tseem hu ua C-peptide, yog ib qho peptide fragment tsim thaum lub sij hawm insulin synthesis thiab secretion. Raws li pancreatic islet C-peptide, qhov taw qhia tseem ceeb ntawm kev ua haujlwm ntawm tes, muaj ntau yam kev ua haujlwm lom neeg thiab muaj peev xwm siv tshuaj kho mob.
C-PEPTIDE thiab insulin synthesis thiab secretion
C-peptide thiab insulin coexist nyob rau hauv sib npaug molecular tooj nyob rau hauv lub proinsulin molecule thiab sib koom tso tawm rau hauv cov hlab ntsha. Hauv cov txiav hauv cov hlwb, proinsulin yog thawj zaug nkag rau hauv C-peptide thiab insulin los ntawm cov enzymes, thiab tom qab ntawd ob leeg nkag mus rau cov hlab ntsha los ntawm equimolar secretion. Yog li, qib ntawm C-peptide tuaj yeem cuam tshuam cov islets ntawm pancreatic Lub peev xwm ntawm cov hlwb los ua ke thiab tso tawm insulin tau dhau los ua qhov taw qhia tseem ceeb rau kev ntsuas kev ua haujlwm ntawm pancreatic.
(1) Kev faib tawm thiab kev kuaj mob ntshav qab zib
Kev txiav txim siab ntawm qib C-peptide hauv cov ntshav yog pab tau rau kev faib thiab kuaj ntshav qab zib. Hom 1 mob ntshav qab zib mellitus vim yog pancreatic islets Cell ua haujlwm raug puas tsuaj loj, thiab qib C-peptide feem ntau qis; Hauv hom 2 mob ntshav qab zib mellitus, pancreatic islets Cellular muaj nuj nqi tseem muaj, thiab qib C-peptide tej zaum yuav zoo li qub lossis nce me ntsis. Yog li ntawd, kev tshawb pom ntawm C-peptide yog pab kom paub qhov txawv ntawm hom 1 thiab hom 2 mob ntshav qab zib thiab muab lub hauv paus rau kev tsim cov phiaj xwm kho mob.
(2) Ntsuam xyuas pancreatic islets Cellular muaj nuj nqi
Cov qib C-peptide tuaj yeem cuam tshuam cov islets ntawm pancreatic secretion ua haujlwm ntawm cov hlwb. Hauv cov neeg mob ntshav qab zib mellitus, pancreatic islets tuaj yeem raug soj ntsuam los ntawm kev soj ntsuam kev hloov pauv ntawm C-peptide theem Kev txhim kho lossis kev ua haujlwm ntawm tes tsis zoo, txhawm rau kho cov phiaj xwm kho mob thiab txhim kho cov nyhuv kho.
(1) Qhia cov tshuaj insulin
Rau cov neeg mob ntshav qab zib uas xav tau kev kho mob insulin, qib C-peptide tuaj yeem siv los ua qhov ntsuas ntsuas los kho cov tshuaj insulin. Los ntawm kev saib xyuas cov qib C-peptide, cov kws kho mob tuaj yeem tsim kho cov tshuaj insulin tus kheej raws li tus neeg mob lub siab ua haujlwm kom tsis txhob muaj kev phiv xws li hypoglycemia.
(2) Kev kwv yees qhov kev pheej hmoo ntawm kev mob ntshav qab zib
Cov qib C-peptide tuaj yeem cuam tshuam nrog kev pheej hmoo ntawm kev mob ntshav qab zib. Cov kev tshawb fawb tau pom tias qhov qis ntawm C-peptide tuaj yeem cuam tshuam nrog kev pheej hmoo ntawm cov teeb meem xws li ntshav qab zib retinopathy thiab ntshav qab zib nephropathy. Yog li ntawd, kev saib xyuas qib C-peptide tuaj yeem kwv yees qhov kev pheej hmoo ntawm kev mob ntshav qab zib mellitus, siv cov kev cuam tshuam ua ntej, thiab txo qhov tshwm sim ntawm cov teeb meem.
Lwm cov nta ntawm C-PEPTIDE
(1) Txhawb cov roj metabolism
C-peptide muaj qee yam kev tswj hwm ntawm cov rog metabolism. Nws tuaj yeem txhawb oxidation thiab decomposition ntawm fatty acids, txo cov ntshav lipid, thiab pab tiv thaiv thiab kho cov kab mob metabolic xws li kab mob plawv.
(2) Anti-inflammatory teebmeem
Nyob rau hauv xyoo tas los no, kev tshawb fawb tau pom tias C-peptide kuj muaj qee yam tshuaj tiv thaiv kab mob. Nws muaj peev xwm inhibit qhov tsim thiab tso tawm ntawm inflammatory yam, alleviate inflammatory cov tshuaj tiv thaiv, thiab muaj tej yam nqi peev xwm nyob rau hauv kev kho mob ntawm inflammatory kab mob.
5. Kev tshawb pom tshiab ntawm C-PEPTIDE hauv kev tshawb fawb soj ntsuam
(3) Cov nyhuv neuroprotective
C-peptide kuj muaj qee yam kev tiv thaiv ntawm lub paj hlwb. Nws tuaj yeem txhawb kev loj hlob thiab kev sib txawv ntawm cov neurons, inhibit neuronal apoptosis, thiab muaj qee yam tseem ceeb rau kev tiv thaiv thiab kho cov kab mob neurological.
Nrog rau kev tshawb fawb tob zuj zus ntawm C-peptide, kev tshawb pom tshiab hauv kev tshawb fawb soj ntsuam kuj tseem tshwm sim. Piv txwv li, cov kev tshawb fawb tau pom tias C-peptide tuaj yeem cuam tshuam nrog cov kab mob thiab kev kho mob ntawm cov kab mob plawv, rog rog, kev paub tsis meej, thiab lwm yam kab mob. Cov kev tshawb pom tshiab no muab kev cia siab dav dav rau kev siv tshuaj kho mob C-peptide.
C Peptide, tseem hu ua C-peptide, yog ib qho peptide fragment tsim thaum lub sij hawm insulin synthesis thiab secretion. Vim nws txoj haujlwm tseem ceeb ntawm cov roj ntsha tseem ceeb thiab cov kev siv tshuaj kho mob muaj peev xwm, cov synthesis ntawm C-peptide ib txwm yog qhov kev tshawb fawb hotspot nyob rau hauv biochemistry thiab kws tshuaj chemistry.
1. Laboratory synthesis method of C-PEPTIDE
Lub chaw soj nstuam ntawm C-peptides feem ntau yog siv Cov Khoom Siv Phase Peptide Synthesis (SPPS), uas yog ib txoj hauv kev zoo thiab tswj tau peptide synthesis. Lub hauv paus ntawm SPPS txoj kev yog txuas cov amino acids ib tug los ntawm ib tug mus rau ib tug khoom-theem cov cab kuj, thiab los ntawm tas li repetition ntawm cov txheej txheem no, thaum kawg synthesize lub hom phiaj peptide. Cov hauv qab no yog cov kauj ruam tshwj xeeb rau C-peptide synthesis:
(1) Npaj cov khoom thauj khoom theem
Cov khoom thauj khoom feem ntau siv cov polystyrene resin, uas txuas nrog cov pab pawg nquag xws li chloromethyl thiab amino pawg. Cov pab pawg nquag no tuaj yeem cuam tshuam nrog cov carboxyl lossis amino pawg ntawm cov amino acids, yog li kho cov amino acids ntawm cov cab kuj.
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(2) Txuas cov amino acids ib qho los ntawm ib qho
Pib los ntawm N-terminus ntawm C-peptide, txuas cov amino acids ib tug los ntawm ib tug mus rau lub solid-phase carrier. Cov kauj ruam no feem ntau yog ua tiav los ntawm cov tshuaj tiv thaiv ntawm N-tiv thaiv pab pawg ntawm cov amino acid (xws li Boc lossis Fmoc) thiab pab pawg ua haujlwm ntawm cov khoom thauj khoom-theem. Tom qab cov tshuaj tiv thaiv tiav, nws yog ib qho tsim nyog yuav tsum tshem tawm cov pab pawg tiv thaiv kom txuas cov amino acid tom ntej.
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(3) Cyclic tshuaj tiv thaiv
Rov ua cov kauj ruam saum toj no thiab txuas cov amino acids ntxiv ib los ntawm ib qho mus rau peptide chains twb txuas nrog cov cab kuj. Rau txhua tus amino acid txuas nrog, yuav tsum muaj kev tiv thaiv pab pawg tshem tawm thiab ua haujlwm ntxuav kom ntseeg tau qhov tseeb thiab ua haujlwm ntawm cov tshuaj tiv thaiv.
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(4) Txiav peptides los ntawm cov khoom thauj khoom
Tom qab tag nrho cov amino acids txuas nrog, cov peptide yog cleaved los ntawm cov khoom siv-theem uas siv cov cleavage reagents. Cov kauj ruam no feem ntau yog siv cov tshuaj reagents xws li cov kua qaub muaj zog lossis cov hauv paus.
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(5) Purification thiab characterization
Cov peptides synthesized tau purified los ntawm txoj kev xws li high-performance kua chromatography (HPLC) kom tshem tawm impurities thiab unreacted amino acids. Tom qab ntawd, cov purified peptide yog tus cwj pwm siv cov tswv yim xws li huab hwm coj spectrometry (MS) thiab nuclear magnetic resonance (NMR) kom paub meej tias nws cov qauv thiab purity.
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2. Kev sib npaug tshuaj rau kev sib txuas ntawm C-PEPTIDE
Noj cov khoom-theem peptide synthesis txoj kev ua piv txwv, cov tshuaj sib npaug rauC Peptidesynthesis tuaj yeem qhia tau raws li cov kev sib txuam sib txuam. Ntawm no, ib qho kev sib npaug ntawm cov tshuaj yooj yim rau ib qho ntawm cov kauj ruam raug nthuav tawm los qhia txog lub hauv paus ntsiab lus ntawm C-peptide synthesis.
Piv txwv tias C-peptide ib ntu peb xav ua ke yog Ala Val Pro Gln Gly, thawj kauj ruam yog los txuas thawj cov amino acid (alanine) rau cov khoom thauj khoom:
Cov khoom thauj khoom theem-CH2Cl + Ala-OH → Cov khoom thauj khoom theem-CH2-Ala
Kauj Ruam 2: Txuas qhov thib ob amino acid (valine):
Cov khoom thauj khoom theem-CH2-Ala +Val-OH → Khoom thauj khoom theem-CH2-Ala Val
C-Peptide, tseem hu ua linker peptide, yog zais los ntawm pancreatic beta hlwb thiab sib koom ua ntej nrog insulin, proinsulin. Insulin precursor yog cleaved rau hauv ib lub molecule ntawm insulin thiab ib lub molecule ntawm C-peptide, yog li cov molar tus nqi ntawm C-peptide thiab tus kheej insulin zoo ib yam. Vim tias C-peptide tsis yooj yim degraded los ntawm daim siab, ntsuas C-peptide yog sib npaug los ntsuas cov ntsiab lus insulin, uas tuaj yeem cuam tshuam qhov kev ua haujlwm ntawm pancreatic islet hlwb. Thaum kuaj ntshav qabzib hauv qhov ncauj, ntshav tuaj yeem ntsuas ntsuas cov ntshav C-peptide ntawm 1 teev, 2 teev, thiab 3 teev tom qab yoo ntshav qabzib. Hauv cov tib neeg ib txwm muaj, qib C-peptide nce mus rau ntau dua peb zaug theem pib tom qab 60 feeb ntawm kev noj cov piam thaj. Qib ntawm C-peptide hauv hom 1 mob ntshav qab zib mellitus yog qhov tsawg heev, thiab qhov nce ntawm C-peptide tom qab noj mov hauv cov neeg mob uas muaj cov islet ua haujlwm tsis zoo feem ntau tsawg dua peb zaug. Rau cov neeg mob tau txais kev kho mob insulin, ntsuas cov ntshav insulin tsis tuaj yeem ntsuas lawv tus kheej pancreatic beta cell function. Cov qib C-peptide tuaj yeem ntsuas los ntsuas lawv tus kheej pancreatic beta cell ua haujlwm.
Cia
Txoj kev cia ntawmC Peptideyuav tsum tau ua kom nws ruaj khov thiab ua haujlwm. Cov hauv qab no yog cov lus qhia rau txoj kev cia ntawm C-Peptide raws li ntau qhov chaw:
1.Basic cia tej yam kev mob
- Kub: Nws yog feem ntau pom zoo kom khaws cia hauv qhov chaw kaw thiab tsaus ntuj ntawm 2 degree thiab 8 degree. Qhov no yog los tswj nws cov kev ua haujlwm lom neeg thiab kev ruaj ntseg. Yog tias tsis xav tau lub sijhawm ntev, lub tub yees ntawm -15 degree -20 degree tuaj yeem txiav txim siab kom txuas ntxiv nws lub sijhawm cia.
- Tsis txhob pom lub teeb: Cov khoom no yog rhiab heev rau lub teeb, yog li nws yuav tsum muab cia rau hauv lub thawv kaw thiab muab tso rau hauv qhov chaw tsaus.
2. Cia tom qab qhib
Lub sijhawm siv tau
Hnub tas sij hawm rau cov khoom tsis tau qhib yuav txawv nyob ntawm hom thiab cov chaw tsim khoom, tab sis feem ntau tuaj yeem pom hauv cov khoom siv phau ntawv lossis daim ntawv lo. Tom qab qhib, hnub tas sij hawm yuav luv luv nyob ntawm tus yam ntxwv thiab kev cia khoom ntawm cov khoom. Qee cov khoom yuav muaj lub neej txee tsis pub tsawg tshaj 28 hnub tom qab qhib.
Kev ceev faj cia
Tom qab qhib, cov khoom yuav tsum tau siv sai li sai tau thiab khaws cia nruj me ntsis raws li cov lus qhia hauv phau ntawv qhia khoom. Yog tias cov khoom xav tau siv ntau lub sijhawm, nws tuaj yeem txiav txim siab ntim rau hauv cov thawv me me kom txo tau qhov cuam tshuam ntawm txhua qhov qhib ntawm cov khoom.
3.Storage nyob rau hauv tej daim ntawv thov scenarios
C-peptide hauv cov khoom siv reagent
Yog tias cov khoom siv los ua ib feem ntawm cov khoom siv reagent, nws yuav tsum tau muab khaws cia nruj me ntsis raws li kev cia khoom ntawm cov khoom siv. Piv txwv li, qee cov khoom siv tshuaj ntsuam C-peptide yuav tsum tau khaws cia rau hauv ib puag ncig nrog cov roj corrosive ntawm 2 degree C txog 8 degree C, thiab qhia txog hnub tas sij hawm rau cov khoom tsis tau qhib thiab qhib.
C-peptide hauv cov qauv sib txawv
Hauv chav kuaj, nws yuav raug muab rho tawm los ntawm ntau yam qauv xws li ntshav, ntshav, zis, thiab lwm yam. Cov kev cia ntawm cov qauv no kuj yuav cuam tshuam rau kev ruaj ntseg ntawm cov khoom. Piv txwv li, cov ntshav ntshav los yog cov ntshav plasma feem ntau pom zoo kom khaws cia ntawm -20 degree lossis -80 degree rau lub sijhawm ntev dua kom tsis txhob rov qab khov-thaw.
4.Summary of Storage Precautions
- Xyuas kom meej tias nws yog khaws cia nyob rau hauv qhov pom zoo cia qhov kub thiab txias.
- Tsis txhob muab tso rau hauv tshav ntuj ncaj qha lossis qhov chaw kub kub.
- Tom qab qhib, cov khoom yuav tsum tau siv sai li sai tau thiab khaws cia nruj me ntsis raws li cov khoom siv phau ntawv.
- Yog tias nws tau muab rho tawm los ntawm cov qauv, nws yuav tsum tau ua kom ntseeg tau tias qhov chaw cia ntawm cov qauv ua tau raws li qhov xav tau ntawm cov khoom siv.
Cim npe nrov: c peptide cas 39016-05-2, lwm tus neeg, manufacturers, hoobkas, lag luam wholesale, yuav, nqe, tej, kev muag khoom