Larazotide peptide

In solid-phase peptide synthesis, the first step is to select a suitable resin as the solid-phase carrier. Feem ntau siv cov resins suav nrog Wang Resin, Rink Resin, thiab lwm yam. Lawv muaj cov khoom sib txawv, thiab tuaj yeem xaiv raws li cov khoom ntawm lub hom phiaj peptide. Tso lub cob muaj nyob rau hauv ib tug reactor, ntxiv tus nqi tsim nyog ntawm dichhloromethane (DMF) rau kev offething xws li outino pawg ntawm lub resin rau cov tshuaj tiv thaiv txuas ntxiv.

Dissolve cov amino acids nrog pab pawg tiv thaiv (xws li FMOC Gly Oh, FMOC Val Oh, thiab lwm yam), thiab ntxiv rau ib tug reactor uas muaj cov cob qhia. Raws li qhov tsim nyog kub (feem ntau yog chav tsev) thiab dirring tej yam kev mob, amino acids yauv nrog cov pab pawg uas muaj amino. Tom qab kev sib tw sib txuas yog ua tiav, ntxuav cov chaw so nrog DMF kom tshem tawm cov amino acids uas tsis paub thiab reagents.

Siv pyridine lossis hexahydropyridine kom tshem tawm cov FMOC lossis BOC tiv thaiv pab pawg ntawm cov amino acid tshiab, tso cai rau cov amino acid tom ntej. Rov ua dua cov kauj ruam saum toj no thiab cov khoom siv tsis txaus ntseeg, valine, Valine, Valine, Valine, Valine, thiab Gluthester, thiab Glthesis ntawm tag nrho cov synazotide peptide saw ua tiav.

Tom qab tag nrho cov amino acids tau raug txuas nrog, tshem tawm cov tshuaj tiv thaiv ntawm chav tsev uas muaj cov khoom siv roj hmab thiab cov pab pawg tiv thaiv ntawm cov amino acid sab saw. Tom qab txiav cov tshuaj tiv thaiv tiav, lim thiab tshem tawm cov cob, thiab sau cov ntawv cuav. Qhov filtrate tau precipitated nyob rau hauv dej khov ether thiab centrifuged kom tau txais cov neeg nyoos peptide. Cov neeg nyoos Peptide tau raug ntxuav los ntawm kev ua kom zoo kua chromatography (HPLC) kom tshem tawm impurities thiab los ntawm he}} purity {5} purity.

Xaiv ib lub tswv yim tiv thaiv tsim nyog thiab faib cov saw larazotives mus rau ntau cov neeg luv luv, uas yog cov lus sib cais cais nyob rau theem ua kua. Piv txwv li, me me Fragments xws li dipeptides thiab truneptides tuaj yeem ua ke ua ntej. Thaum lub sij hawm synthesis cov txheej txheem, amino acids nrog pab pawg tiv thaiv yog maj ua txuas nyob rau hauv txoj kev ua ntawm cov kev ntsuas uas tsim nyog (DCM, nyob rau hauv kev ua ntawm kev ua ntawm cov kev ua ntawm coupling thiab cov kev ua kom zoo rau cov hom phiaj. Tom qab txhua qhov tawg yog synthesized, nws yuav tsum tau ua kom huv thiab cov xeeb ceem kom ntseeg tau tias nws cov qauv huv thiab yog qhov tseeb.

Conjugate synthesized tawg hauv cov theem ua kua kom tau cov saw laraxotide tiav. Fragment coupling feem ntau siv cov txheej txheem xws li ua kom anter exter txoj kev thiab sib xyaw hom kev sib xyaw. Piv txwv li, hloov cov pab pawg carboxyl ntawm ib qho zuj zus rau hauv ib pawg ua kom tau zoo, thiab tom qab ntawd reacting nrog cov pab pawg amino los ua daim ntawv cog lus peptide. Tom qab cov tshuaj ntsuam sib txuas yog ua tiav, cov khoom yuav tsum tau ua kom huv thiab cov xeeb ceem kom tshem tawm cov tawg thiab los ntawm {{} cov khoom.

Faib ntau ntawm cov hlaws dai rau hauv ob peb qhov sib npaug, thiab txuas ib qho kev sib txawv amino acid lossis tawg rau txhua tus resin hlaws. Tom qab ntawd, sib xyaw cov hlaws dai ua ke thiab npaj mus rau cov tshuaj tiv thaiv tom ntej. Los ntawm ntau cov txheej txheem ntawm segmentation, synthesis, thiab tov, muaj ntau tus lej ntawm cov analogs tuaj yeem ua tau zoo nyob rau lub sijhawm luv luv.

Tom qab synthesis, tshuaj ntsuam cov peptide lub tsev qiv ntawv rau larazotide analogs nrog cov kev ua haujlwm lom neeg. Cov hau kev tshuaj ntsuam tuaj yeem siv cov txuj ci xws li kev ua haujlwm roj ntsha assay thiab siab-} Kev ntsuas kev ntsuas. Cov qauv kev qhia thiab kev tshawb fawb ntxiv ntawm kev tshuaj ntsuam nquag muab cov lus sib txuas lus zoo rau cov qauv kev txhim kho thiab kev tsim tshuaj ntawm laraazotide.