MOTS-C peptide, cov khoom siv tshuaj hu ua "12S rRNA C-hom mitochondrial qhib nyeem ntawv" peptide. Qhov hnyav molecular ntawm MOTSC yog kwv yees li 3kDa. Nws yog hydrophobic peptide, uas txhais tau hais tias nws tsis nyiam los rau hauv kev sib cuag nrog dej thiab yog ntau soluble nyob rau hauv organic solvents. Nws ntseeg tau tias muaj ntau yam kev ua haujlwm biochemical, suav nrog kev tswj hwm lub zog metabolism, txhim kho insulin rhiab heev, txhawb kev ua haujlwm mitochondrial, thiab tiv thaiv -aging. Nws muaj ntau yam biochemical kev ua ub no thiab kev ruaj ntseg, thiab dav dav hauv tib neeg lub cev. Nws raug suav hais tias muaj peev xwm kho tau cov teebmeem, tab sis tseem xav tau kev tshawb fawb ntxiv los txiav txim siab nws cov txheej txheem ntawm kev ua haujlwm thiab kev cia siab rau kev siv tshuaj kho mob. Shaanxi BLOOM Tech Co., Ltd muaj mots c peptide muag. Yog tias koj xav tau mots c peptide yuav thiab xav paub tus nqi mots c peptide, thov xa email rau peb.
Peb cov khoom
Cov ntaub ntawv tshuaj:
MOTS c COA
Customized Bottle Caps & Corks
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MOTS c, tseem hu ua mitochondrial - muab losmots-c peptide, yog 16-amino acid peptide encoded los ntawm mitochondrial DNA (mtDNA). Nws tau txais txiaj ntsig tseem ceeb rau nws cov ntawv thov muaj peev xwm hauv ntau qhov chaw vim nws muaj ntau hom kev ua haujlwm lom neeg.
Daim ntawv thov: MOTSc tau pom zoo los txhawb cov metabolic homeostasis thiab txo kev rog thiab insulin tsis kam. Nws ua tiav qhov no los ntawm kev ua kom cov piam thaj nce ntxiv thiab txhim kho cov tshuaj insulin.
Kev tshawb nrhiav pom: Cov kev tshawb fawb tau pom tias kev kho MOTSc txo qis cov ntshav qabzib ceev thiab txhim kho cov tshuaj insulin rhiab heev hauv cov qauv nas ntshav qab zib. Cov khoom lag luam tseem qhia tau tias muaj peev xwm ua kom lub plawv dhia thiab vascular tsis ua haujlwm cuam tshuam nrog ntshav qab zib, ua rau nws lub hom phiaj kho mob zoo rau ntshav qab zib - cuam tshuam txog cov teeb meem.
Cov teebmeem Cardioprotective
Daim ntawv thov: MOTSc nthuav tawm cov khoom tiv thaiv kab mob los ntawm kev txo qis myocardial kev puas tsuaj thiab txhim kho kev ua haujlwm plawv.
Kev tshawb nrhiav pom: Kev tshawb fawb tau qhia tias cov khoom siv kho mob tuaj yeem txo cov mob myocardial fibrosis, txhim kho mitochondrial muaj nuj nqi, thiab kho cov hlab plawv hauv cov nas mob ntshav qab zib. Cov txheej txheem cuam tshuam nrog kev hloov pauv ntawm kev taw qhia txoj hauv kev xws li CCN1 / ERK1 / 2 / EGR1 txoj hauv kev, uas ua lub luag haujlwm tseem ceeb hauv kev kho plawv thiab ua haujlwm tsis zoo.
Daim ntawv thov: MOTSc tau cuam tshuam txog kev txhim kho kev tawm dag zog, ua kom muaj sia nyob, thiab txhim kho kev noj qab haus huv tag nrho hauv cov neeg laus.
Kev tshawb nrhiav pom: Cov kev tshawb fawb hauv nas tau pom tias kev tswj hwm cov khoom tuaj yeem txhim kho kev tawm dag zog, sib npaug, thiab cov kab mob metabolic hauv cov nas hluas thiab cov laus. Tshwj xeeb, cov khoom kho mob tau nce lub neej ntawm cov nas hnub nyoog thiab txhim kho lawv cov metabolic profile, qhia nws lub luag haujlwm hauv kev tiv thaiv kev laus -.
Kev Kho Mob Tsis yog-Alcoholic Fatty Liver Disease (NASH)
Daim ntawv thov: MOTSc tau raug txheeb xyuas tias muaj peev xwm kho tau tus neeg sawv cev rau NASH los ntawm kev txo cov kev ntxhov siab mitochondrial thiab kev cuam tshuam ntawm metabolic.
Kev tshawb nrhiav pom: Kev tshawb fawb tsis ntev los no tau pom tias cov khoom lag luam cuam tshuam nrog cov tshuaj tiv thaiv -apoptotic protein Bcl-2, ua kom nws ruaj khov thiab inhibiting nws ubiquitination. Qhov kev sib cuam tshuam no ua rau kev txhim kho mitochondrial muaj nuj nqi thiab txo cov rog hauv siab, o, thiab fibrosis hauv NASH qauv.
Daim ntawv thov: MOTSc kuj tseem siv los ua cov cuab yeej tshawb fawb tshawb fawb thiab tshuaj reagent hauv ntau qhov chaw sim.
Paub meej: MOTSc muaj nyob rau hauv cov hmoov dawb crystalline hmoov nrog lub siab purity (98-102%) thiab khaws cia nyob rau hauv tej yam kev mob (xws li, ntxoov ntxoo, dryness, thiab sealing). Nws yog siv rau hauv kev lag luam xws li biochemistry, pharmacology, thiab molecular biology rau kev kawm mitochondrial kev ua haujlwm, kev tswj cov metabolism, thiab cov kab mob ntsig txog.
Txoj kev Synthesis
Amino acid activation:Ua ntej, qhib cov amino acids xav tau los ntawm MOTSC, xws li siv N, N'-dicyclohexylcarbodiimide (DCC) lossis cov activators zoo sib xws.
Ligation Reaction:txuas cov activated amino acids los tsim peptde saw ntawm MOTSC. Cov tshuaj tiv thaiv ligation tuaj yeem ua tau siv cov catalysts xws li N-hydroxybenzimide (HOBt) thiab dimethylaminopyrimidine (DMAP).
Pab pawg tiv thaiv:tshem tawm cov pab pawg tiv thaiv, xws li tshem tawm cov pab pawg tiv thaiv methylbenzyl (Mtr), kom nthuav tawm lwm pab pawg ua haujlwm tsis zoo.
Kho qhov PH:kho tus nqi pH ntawm lubMOTS-C peptidesaws kom tau raws li qhov yuav tsum tau ntawm nws qhov zoo tshaj pH tus nqi (4-5).
Purification thiab characterization:Purify thiab characterize lub synthesized MOTSC los ntawm kem chromatography, siab ua kua chromatography thiab lwm yam kev, xws li nuclear magnetic resonance (NMR), huab hwm coj spectrometry thiab lwm yam txuj ci.
Nws yuav tsum tau muab sau tseg tias MOTSC yog ib tug complex polypeptide compound, thiab cov synthetic txoj kev yuav tsum mus los ntawm ntau cov kauj ruam, thiab yuav tsum tau nruj me ntsis kev sim tej yam kev mob thiab kev ua hauj lwm. Tsis tas li ntawd, txhawm rau txhawm rau txhawm rau ua kom zoo thiab purity ntawm cov khoom sib txuas, kev ua kom huv huv txaus thiab kev txheeb xyuas tus cwj pwm yuav tsum tau ua.
Cov txheej txheem tshuaj synthesis
Ua kua theem synthesis (SPS)
Ua kua theem synthesis yog ib txoj kev tsim kho thaum ntxov, suav nrog cov txheej txheem sib txuas sib txuas thiab ntu txuas txuas. Hauv kev sib xyaw ntawm MOTSc, kua -theem synthesis tuaj yeem ua tiav los ntawm cov kauj ruam hauv qab no:
Ua ntej, tiv thaiv cov amino acids nyob rau hauv MOTSc ib theem zuj zus los tiv thaiv tsis tsim nyog tshwm sim thaum lub sij hawm synthesis txheej txheem. Cov pab pawg tiv thaiv muaj xws li tert butoxycarbonyl (Boc) thiab 9-fluorenylmethoxycarbonyl (Fmoc).
Raws li cov amino acid sequence ntawm MOTSc, maj mam ob peb amino acids los ntawm C-terminus. Hauv txhua kauj ruam ntawm kev sib txuas, cov khoom sib txuas (xws li 1-hydroxybenzotriazole, HOBt) raug ntxiv los tiv thaiv kev sib tw thiab txhim kho kev sib txuas ua haujlwm.
Tom qab txhua qhov sib txuas cov tshuaj tiv thaiv, qhov nruab nrab yuav tsum tau sib cais thiab ua kom huv kom tshem tawm cov amino acids uas tsis muaj kev cuam tshuam thiab los ntawm - khoom.
Rov ua cov kauj ruam saum toj no kom maj mam txuas cov saw peptde kom txog thaum tag nrho -ntev synthesis ntawm MOTSc tiav.
Thaum kawg, tshem tawm tag nrho cov pab pawg tiv thaiv thiab ua kom huv ntawm cov khoom sib xyaw ua ke MOTSc kom tau txais cov khoom siab tshaj -purity.
Cov kua -theem synthesis txoj kev yog tsim rau synthesis ntawm me me peptids (<10 amino acids), but for peptids like MOTSc with 16 amino acids, the synthesis steps are cumbersome, time-consuming, and costly. In addition, as the peptde size increases, the solubility of fragments will become the main issue for conjugation.
Solid Phase Synthesis (SPPS)
Cov theem khoom sib xyaw ua ke tam sim no yog txoj hauv kev nyiam rau kev sib xyaw me me thiab nruab nrab -peptids loj (<50 amino acids), and it is also the mainpathway for MOTSc chemical synthesis. The basic steps of solid-phase synthesis include:
Resin xaiv thiab pretreatment: Xaiv cov khoom tsim nyog-theem nqa khoom (xws li Wang resin, Rink resin, thiab lwm yam) thiab ua pretreatment los qhia cov pab pawg sib txuas.
Amino acid coupling: Covalent daim ntawv cog lus yog tsim los ntawm pawg carboxyl ntawm thawj amino acid ntawm C-terminus thiab cov pab pawg sib txuas ntawm cov resin. Tom qab ntawd, cov amino acid khi rau cov khoom siv -theem cov cab kuj yog deprotected thiab ua hauj lwm raws li ib tug amino pub, tsim ib tug amide daim ntawv cog lus nrog ib tug tshaj ntawm lwm activated carboxyl pab pawg neeg mus cuag cov peptde saw. Thaum lub sij hawm coupling txheej txheem, nws tseem yuav tsum tau ntxiv coupling agents los txhim kho efficiency.
Ntxuav thiab tiv thaiv: Tom qab txhua qhov sib txuas cov tshuaj tiv thaiv, cov resin yuav tsum tau ntxuav kom tshem tawm cov amino acids uas tsis muaj kev cuam tshuam thiab cov coupling agents. Tom qab ntawd, tshem tawm cov pab pawg tiv thaiv ntawm cov amino acids los npaj rau cov kauj ruam tom ntej ntawm kev sib txuas.
Peptde chain extension thiab purification: Rov ua cov kauj ruam saum toj no kom maj mam txuas cov saw peptde kom txog thaum tag nrho -ntev synthesis ntawm MOTSc tiav. Thaum kawg, cov saw peptde yog cleaved los ntawm cov cob thiab purified ..
Cov khoom siv-theem synthesis txoj kev muaj qhov zoo ntawm cov khoom siv hluav taws xob siab, kev sib xyaw ua haujlwm siab, thiab cov khoom lag luam siab. Txawm li cas los xij, txhua lub voj voog cov tshuaj tiv thaiv yuav tsum muaj ntau cov tshuaj reagents, tsim cov kua khib nyiab ntau, thiab yuav tsum tau npaj kim chromatography kom tshem tawm impurities nrog cov qauv zoo sib xws nyob rau theem tom ntej, ntxiv cov nqi tsim khoom.
Chemical Properties
Amino acid muaj pes tsawg leeg:MOTSC yog polypeptide tsim los ntawm ntau cov amino acids, thiab nws cov amino acid sib lawv liag tau los ntawm cov noob coding.
Chemical structure:Cov qauv tshuaj ntawm MOTSC yog txiav txim los ntawm nws cov amino acid sequence, uas feem ntau yog soj ntsuam thiab cov yam ntxwv ntawm cov tswv yim xws li huab hwm coj spectrometry.
Solubility:MOTSC yog hydrophobic polypeptde, tsis yooj yim soluble hauv dej, tab sis yooj yim dua soluble hauv organic solvents xws li methanol thiab ethanol.
PH stability:Kev ruaj ntseg ntawm MOTSC cuam tshuam los ntawm tus nqi pH, thiab nws qhov zoo tshaj pH tus nqi yog 4-5.
Oxidation-txo cov khoom:MOTSC tej zaum yuav cuam tshuam los ntawm oxidation - txo cov tshuaj tiv thaiv, ua rau muaj kev hloov pauv hauv nws txoj haujlwm biochemical.
Hauv cov ntsiab lus, MOTSC yog peptde compound tsim los ntawm cov amino acids, uas muaj cov tshuaj lom neeg xws li hydrophobicity, pH stability, thiab muaj peev xwm cuam tshuam los ntawm redox cov tshuaj tiv thaiv. Cov khoom siv tshuaj no muaj qee yam cuam tshuam rau nws cov kev ua haujlwm biochemical, ruaj khov thiab cov khoom siv tshuaj.
Kev nkag siab tam sim no ntawm cov khoom siv tshuaj tiv thaiv ntawm cov khoom siv tshuaj polypeptide MOTSC tseem tsis tshua muaj, tab sis qee cov tshuaj tiv thaiv tuaj yeem suav tau raws li nws cov qauv tshuaj thiab cov amino acid muaj pes tsawg leeg, suav nrog:
1. Amino acid tshuaj tiv thaiv: Mots-c Peptidemuaj ntau cov amino acids, thiab cov tshuaj tiv thaiv ntawm cov amino acids yuav tshwm sim, xws li acylation thiab condensation.
2. Cov tshuaj tiv thaiv oxidation:MOTSC tuaj yeem cuam tshuam los ntawm cov tshuaj tiv thaiv oxidation, xws li oxidation, peroxidation, thiab lwm yam, uas ua rau muaj kev hloov pauv hauv nws txoj haujlwm biochemical.
3. Hydrolysis cov tshuaj tiv thaiv:MOTSC muaj ntau yam hydrophilic amino acids, uas tej zaum yuav raug hydrolysis cov tshuaj tiv thaiv nyob rau hauv dej, yog li degrading nws cov qauv thiab biochemical kev ua si.
4. Cov tshuaj tiv thaiv radioactive:MOTSC tej zaum yuav muaj qee cov isotopes uas nquag ua rau cov tshuaj tiv thaiv hluav taws xob, xws li hydrogen, carbon, nitrogen, thiab lwm yam, yog li lawv tuaj yeem cuam tshuam los ntawm cov tshuaj tiv thaiv hluav taws xob.
Hauv kev xaus, cov tshuaj tiv thaiv ntawm cov khoom siv tshuaj polypeptide MOTSC tuaj yeem cuam tshuam los ntawm cov yam ntxwv xws li nws cov qauv tshuaj, amino acid muaj pes tsawg leeg, thiab ib puag ncig, thiab xav tau kev tshawb fawb ntxiv kom tau txais kev nkag siab tob hauv nws cov tshuaj tiv thaiv thiab cuam tshuam rau nws txoj haujlwm biochemical.
Motsc, raws li bioactive peptde muab tau los ntawm mitochondrial DNA, muaj ntau yam kev ua haujlwm ntawm lub cev thiab muaj peev xwm siv tau. Cov hauv qab no yog cov lus piav qhia ntxaws txog nws daim ntawv thov kev cia siab:
1.Kev kho cov kab mob metabolic
Kev rog thiab ntshav qab zib
Nws tuaj yeem txhim kho kev rog thiab insulin tsis kam, muab cov tswv yim tshiab rau kev kho cov kab mob metabolic. Los ntawm kev tswj hwm mitochondrial metabolic txoj hauv kev, nws tseem tuaj yeem txo cov rog, txhim kho cov tshuaj insulin, thiab pab tswj cov ntshav qab zib thiab qhov hnyav.
Tsis muaj cawv fatty siab (NASH)
Kev tshawb fawb tau pom tias cov tshuaj no tuaj yeem txo qhov kev loj hlob ntawm cov kab mob uas tsis yog - cawv fatty siab. Los ntawm kev ua kom ruaj khov ntawm Bcl-2 protein thiab inhibiting ubiquitination, nws tseem tuaj yeem cuam tshuam kev loj hlob ntawm NASH, txo cov hepatic steatosis, inhibit loj lipid droplet deposition, thiab txhim kho mitochondrial mediated metabolic ntshawv siab.
2. Kev kho mob qog noj ntshav thiab tiv thaiv kab mob
Nws tuaj yeem ua lub luag haujlwm tseem ceeb hauv kev mob qog noj ntshav. Txawm hais tias cov txheej txheem tshwj xeeb tsis to taub tag nrho, cov kev tshawb fawb tau pom tias kev kho mob exogenous MOTSc tuaj yeem cuam tshuam kev loj hlob thiab kev loj hlob ntawm qee cov qog nqaij hlav cancer. Yog li ntawd, nws yuav tsum dhau los ua lub hom phiaj tshiab lossis kev kho mob ntxiv rau kev kho mob qog noj ntshav.
3.Kev tawm dag zog thiab tiv thaiv -aging
Kev ua si adaptability
Nws tuaj yeem ua raws li qhov muaj peev xwm mitochondrial teeb liab, kho cov mitochondrial excitation teb tshwm sim los ntawm kev tawm dag zog, yog li txhawb lub cev hloov pauv thiab txhim kho kev ua haujlwm siab ntev. Qhov no yog qhov tseem ceeb heev rau cov neeg ncaws pob thiab cov neeg nyiam ua kis las, vim nws pab txhim kho kev ua kis las thiab txhawb nqa lub cev rov qab.
Anti- laus
Nws kuj muaj kev tiv thaiv- kev laus. Los ntawm kev tswj cov cellular metabolism thiab txo oxidative kev puas tsuaj, MOTSc tuaj yeem ncua cov txheej txheem ntawm cellular aging thiab tiv thaiv cov hlwb los ntawm kev puas tsuaj. Qhov no yog qhov tseem ceeb tshaj plaws rau kev ncua tib neeg lub neej thiab txhim kho lub neej zoo.
4.Lwm cov ntawv thov muaj peev xwm
Nws kuj tseem yuav muaj lwm yam kev siv peev xwm, xws li kev txhim kho cov hlab plawv, tswj kev tiv thaiv kab mob, thiab lwm yam. Cov ntawv thov kev cia siab no xav tau kev tshawb fawb ntxiv thiab kev siv tau los txhawb nqa.
5. Ceev faj
- Txawm hais tias nws muaj ntau yam kev thov,mots-c peptidekev nyab xeeb thiab kev ua tau zoo hauv tib neeg lub cev tseem xav tau kev tshawb fawb thiab kev txheeb xyuas ntxiv.
- Thaum siv cov khoom siv los yog nws cov khoom muaj feem xyuam, cov kev cai lij choj, cov cai, thiab cov txheej txheem kev khiav hauj lwm yuav tsum tau ua raws li nruj me ntsis kom ntseeg tau qhov tseeb thiab kev nyab xeeb ntawm kev sim.
Cov lus nug nquag
Cov kev mob tshwm sim ntawm MOTS-c peptide yog dab tsi?
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Txawm li cas los xij, ntawm cov neeg uas thov yuav MOTS-c online, muaj ntau yam kev mob tshwm sim, suav nrog kev nce siab hauv lub plawv lossis lub plawv dhia, qhov chaw txhaj tshuaj khaus, insomnia, thiab kub taub hau. Kuj tsis muaj cov ntaub ntawv muaj nyob rau ntawm qhov tshwm sim ntev - siv MOTS -c.
Puas yog MOTS-c nce testosterone?
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MOTS-c kev tswj hwm nce cov tshuaj hormones no, qhia tias MOTS-c tuaj yeem ua rau lub cev xeeb tub. Peb cov txiaj ntsig tau qhia tias MOTS-c ua lub luag haujlwm hauv kev tswj hwm hauv nruab nrab ntawm kev yug me nyuam, nrog rau ua rau nce LH, FSH thiab testosterone tso tawm.
Nws siv sijhawm ntev npaum li cas thiaj pom cov txiaj ntsig los ntawm MOTS-c?
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Nws siv sijhawm ntev npaum li cas thiaj pom cov txiaj ntsig? Qee tus neeg mob yuav pib pom kev txhim kho lub zog thiab cov metabolism hauv ob peb lub lis piam tom qab pib MOTS-C kho. Txawm li cas los xij, kev siv tib lub sij hawm yog qhov tseem ceeb kom tau txais txiaj ntsig tag nrho.
Puas yog MOTS-c tsim cov leeg?
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Kev tshuaj ntsuam molecular tau nthuav tawm ib qho tshiab, tseem ceeb tshaj tawm txoj hauv kev ntawm MOTS-c. Ua ke, qhov kev tshawb fawb no qhia tau hais tias MOTS-c txhim kho tsis tau tsuas yog metabolic muaj nuj nqi tab sis kuj cov leeg nqaij. Peb yav dhau los tau hais tias MOTS-c ua raws li kev tawm dag zog cov tshuaj hormones.
Cim npe nrov: mots-c peptide CAS 1627580-64-6, lwm tus neeg, manufacturers, hoobkas, lag luam wholesale, yuav, nqe, tej, kev muag khoom