Shaanxi BLOOM Tech Co., Ltd. yog ib qho ntawm cov tuam ntxhab thiab cov muag khoom ntawm hematoxylin hmoov cas 517-28-2 hauv Suav teb. Txais tos rau cov lag luam wholesale bulk zoo hematoxylin hmoov cas 517-28-2 muag ntawm no los ntawm peb lub hoobkas. Kev pabcuam zoo thiab tus nqi tsim nyog muaj.
Hematoxylin hmoov, ib tug natural dye nrog CAS 517-28-2 thiab molecular mis C16H14O6, yog ib tug pigment muab rho tawm los ntawm qhuav ceg ntawm Haematoxylon campechianum nyob rau hauv South America siv ether. Nws yog ib qho ntawm feem ntau siv dyes thiab ib tug natural medium dye. Siv rau dyeing txhob lo lo ntxhuav, pluab, thiab tawv, nrog rau luam ntawv paj rwb fabrics, txawv mordants yuav siv tau kom tau xiav los yog dub xim. Hauv cov kev daws teeb meem aqueous, tshwj xeeb tshaj yog nyob rau hauv cov kev daws teeb meem alkaline, nws yooj yim oxidized los ntawm huab cua los tsim cov xim av xim av oxidized hematoxylin. Hematoxylin tsis tuaj yeem raug stains ncaj qha thiab yuav tsum raug rau thaj chaw uas muaj qhov cua zoo kom tig mus rau oxidized hematoxylin (tseem hu ua hematoxylin) ua ntej nws tuaj yeem siv tau, uas yog hu ua "maturation".
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Tshuaj Formula |
C16H14O6 |
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Exact Mass |
302 |
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Molecular Luj |
302 |
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m/z |
302 (100.0%), 303 (17.3%), 304 (1.4%), 304 (1.2%) |
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Elemental Analysis |
C, 63.58; H, 4.67; O, 31.76 |
Cov txheej txheem maturation ntawm hematoxylin siv sijhawm ntev, thiab qhov ntev nws tau npaj, nws lub zog muaj zog dua. Cov khoom siv dyed yuav tsum tau ua raws li qhov kev txiav txim ntawm cov ntsev hlau ua tus neeg nruab nrab ua ntej nws tuaj yeem muaj zog xim. Yog li thaum npaj hematoxylin dye, yuav tsum tau siv mordant. Cov mordants muaj xws li txhuas sulfate, poov tshuaj alum, thiab hlau alum. Hematoxylin yog lub teeb daj rau xeb xim daj crystalline khoom uas yog insoluble hauv dej txias, ether, thiab glycerol, yooj yim soluble hauv dej kub thiab cawv kub, thiab soluble hauv cov kev daws teeb meem ntawm alkali, ammonia, thiab borax. Nws yog ib qho khoom siv zoo heev rau staining cell nuclei thiab tuaj yeem sib txawv cov qauv sib txawv hauv cov hlwb rau ntau xim.
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Cov xim ntawm cov ntaub so ntswg stained thaum lub sij hawm sib txawv nws txawv nyob ntawm qhov kev kho mob. Tom qab sib txawv nrog cov kua qaub (xws li hydrochloric acid cawv), nws tig liab, thiab tom qab ntxuav nrog dej, nws tseem rov qab mus rau xiav xiav. Tom qab sib txawv nrog cov tshuaj alkaline (xws li dej ammonia), nws tig xiav, thiab tom qab ntxuav nrog dej, nws tig xiav dub. Feem ntau ua rau cov chromatin nyob rau hauv lub nucleus thiab ribosomes nyob rau hauv lub cytoplasm tshwm liab doog xiav; Eosin yog ib qho acidic dye uas feem ntau ua rau cov khoom hauv cytoplasm thiab extracellular matrix tig liab. Nws staining yog cov txheej txheem tseem ceeb tshaj plaws thiab siv dav hauv kev qhia thiab kev tshawb fawb ntawm histology, embryology, thiab pathology.
Hematoxylin hmoovyog ib tug natural polyphenolic alkaline dye muab rho tawm los ntawm lub plawv ntoo ntawm legume cog Eucommia ulmoides. Nws lub ntsiab staining active ingredient yog hematoxylin liab, uas yog tsim los ntawm oxidation thiab maturation. Nrog nws muaj zog affinity rau acidic nucleic acids nyob rau hauv lub chromatin ntawm lub cell nucleus, ruaj staining xim, thiab muaj zog compatibility nrog ntau yam counterstaining agents, nws tau dhau los ua cov feem ntau siv classic staining reagent nyob rau hauv lub teb ntawm histology thiab pathological kuaj nyob rau hauv lub dhau los 150 xyoo. Nws yog ib qho irreplaceable core tivthaiv nyob rau hauv cov pa HE staining system.
Tsis zoo li cov khoom siv hluavtaws aniline dyes, lub hauv paus ntawm hematoxylin stained cells nthuav tawm cov xim zoo nkauj thiab pob tshab xiav ntshav nrog cov xim zoo nkauj. Cov ntaub ntawv hloov maj mam ntawm nucleoli thiab chromatin tuaj yeem nthuav tawm kom meej, thiab nws tuaj yeem hloov mus rau yuav luag txhua hom kev kuaj mob lom neeg los ntawm cov seem paraffin, cov seem khov rau hauv cov cell smears, npog kaum ob ntawm cov xwm txheej xws li kev kuaj mob pathological, kev tshawb fawb lom neeg, kev txheeb xyuas kev tshawb fawb, thiab lwm yam.
Daim ntawv thov ntawm nuclear core staining nyob rau hauv niaj hnub HE staining rau kev kho mob pathology
Qhov tseem ceeb tshaj plaws thiab siv dav siv ntawm hematoxylin yog ib qho tshuaj tua kab mob tshwj xeeb rau kev ua haujlwm niaj hnub HE staining hauv kev kho mob pathology, thiab nws yog feem ntau siv staining reagent hauv txhua lub tsev kho mob pathology txhua hnub. Nyob rau hauv tus txheej txheem staining ntawm cov pa paraffin seem, cov ntaub so ntswg uas tau undergone dewaxing thiab hydration yog immersed nyob rau hauv mature hematoxylin staining tov uas tau stained nrog alum rau 3-5 feeb.
Cov chromatin nyob rau hauv lub cell nucleus tshwj xeeb khi rau hematoxylin aluminium mordant complex thiab yog stained uniformly sib sib zog nqus xiav ntshav. Tom qab ntawd, tom qab eosin counterstaining, cytoplasm thiab intercellular matrix nthuav tawm xim liab. Cov xim muaj zog sib piv ntawm xiav ntshav thiab paj yeeb ua rau thaj tsam morphological, nuclear cytoplasmic ratio, thiab cov ntaub so ntswg theem ntawm cov hlwb kom meej thiab txawv. Pathologists tau yooj yim txheeb xyuas lub hauv paus morphological rau benign thiab malignant kev txiav txim xws li cell heterogeneity thiab nuclear faib.
Cov ntaub ntawv soj ntsuam los ntawm cov chaw kho mob pathology ntawm cov tsev kho mob saum toj kawg nkaus hauv Suav teb qhia tias ntau dua 95% ntawm kev kuaj kab mob niaj hnub tso siab rau HE staining, thiab qhov zoo ntawm hematoxylin staining ncaj qha txiav txim siab qhov kawg ntawm HE ntu. Kev ua kom zoo hematoxylin staining tuaj yeem ua cov qauv nucleolar nrog txoj kab uas hla ntawm tsuas yog 1 μ m meej thiab paub qhov txawv, txo qhov kev pheej hmoo ntawm kev kuaj tsis tau ntxov ntawm -theem microcarcinoma. Hauv qhov xwm txheej ceev staining ntawm intraoperative frozenpathology, kev txhim kho saihematoxylin hmoovstaining tov tuaj yeem compress lub sijhawm staining ntawm cell nuclei kom tsawg dua 1 feeb. Lub contour ntawm stained khov seem cell nucleus yog ntse, tsis muaj teeb meem tshwm sim ntawm qhov muag plooj cell nuclei thiab grey keeb kwm yav dhau nyob rau hauv tsoos sai staining.
Ua kom tau raws li qhov yuav tsum tau ua kom pom sai sai ntawm kev kuaj mob tsis pub dhau 30 feeb ntawm kev ua haujlwm ntawm cov kab mob. Hauv kev kuaj kab mob pathological ntawm cov tsev kho mob thawj zaug, kev ruaj ntseg zoo ntawm hematoxylin staining yog ntxiv rau qhov tseem ceeb. Tsim nyog paub tab hematoxylin staining tov tuaj yeem rov qab siv tau rau ob peb lub hlis ntawm chav tsev kub, thiab cov nyhuv staining yuav tsis pom qhov txo qis. Nws tsis xav tau cov khoom siv staining tsis siv neeg kim, thiab tsuas yog cov khoom siv staining yog xav tau los ua kom tiav qhov kev npaj ntawm cov khoom tsim nyog.
Nws zoo kawg nkaus hloov mus rau cov peev txheej tsawg ntawm cov chaw kuaj kab mob thawj zaug thiab muab cov nqi qis -nqi thiab txhim khu kev qha staining kev daws teeb meem loj rau - nplai mob qog noj ntshav thaum ntxov. Lwm qhov txiaj ntsig tseem ceeb ntawm hematoxylin staining yog tias cov seem stained muaj lub teeb pom kev zoo heev, thiab yuav tsis muaj qhov pom tseeb fading ntawm cell nucleus txawm tias tom qab muab tso rau hauv ib puag ncig sab hauv tsev rau ntau xyoo lawm. Cov HE cov ntaub ntawv khaws tseg los ntawm lub tsev kho mob pathology lub tuam tsev txij li kaum xyoo dhau los tseem tuaj yeem ua kom pom tseeb ntawm morphology ntawm lub cell nucleus, ua tau raws li kev lag luam tus qauv yuav tsum tau ua kom ntev - lub sij hawm archiving thiab retrospective ntawm pathological cov ntaub ntawv. Qhov no yog tus yam ntxwv uas feem ntau ntawm cov khoom siv hluav taws xob tsim hluav taws xob ntawm tes nucleus dyes tsis tuaj yeem ua tiav.
Tshwj xeeb staining siv rau cov ntaub so ntswg tshwj xeeb chemistry thiab lub hom phiaj cov qauv
Ntxiv nrog rau cov pa roj carbon monoxide, hematoxylin tau raug hloov kho nrog cov qauv sib txawv kom tau txais ntau lub tshuab tshwj xeeb staining, ua tiav cov hom phiaj paub txog cov ntaub so ntswg tshwj xeeb thiab cov qauv kab mob, thiab ua si lub luag haujlwm tsis tuaj yeem hloov pauv hauv cov xwm txheej tshwj xeeb ntawm kev kuaj mob. Nyob rau hauv cov ntsiab lus ntawm Nissl staining nyob rau hauv cov ntaub so ntswg neural, hloov kho toluidine xiav hematoxylin ua ke staining system siv alum hematoxylin muaj peev xwm tshwj xeeb stain Nissl nyob rau hauv lub cytoplasm ntawm neurons mus rau hauv tob xiav.
Qhia meej txog kev faib tawm thiab kev hloov pauv ntawm Nissl hauv cytoplasm ntawm neurons. Qhov no pab cov kws kho mob neuropathologist kom paub meej cov kev hloov pauv ntawm cov neurons hauv cov hlab ntsha raug mob thiab cov kab mob neurodegenerative, thiab tau siv dav hauv kev tshawb fawb pathological thiab kev kuaj mob ntawm Alzheimer's disease. Nyob rau hauv cov ntsiab lus ntawm striated nqaij staining nyob rau hauv cov leeg nqaij, phosphotungstic acid hematoxylin (PTAH) tshwj xeeb staining system yog classic tshwj xeeb staining txoj kev muab los ntawm hematoxylin. Kev sib xyaw ntawm hematoxylin thiab phosphotungstic acid nyob rau hauv cov mis no tuaj yeem ua rau cov kab txaij hla ntawm cov leeg pob txha thiab cov txheej txheem sib txuas ntawm myocardium rau hauv cov xim xiav xiav sib sib zog nqus.
Nyob rau tib lub sijhawm, collagen fibers thiab cellulose Cheebtsam nyob rau hauv cov ntaub so ntswg muaj kab mob tuaj yeem ua kom pom tseeb thiab muaj xim, muab cov ntaub ntawv pov thawj pom tau zoo hauv kev kuaj mob ntawm rhabdomyosarcoma thiab myocardial lesions. Nyob rau hauv cov ntsiab lus ntawm cov kab mob ntawm cov kua tsib nyob rau hauv daim siab pathology, kev txhim kho Mayer hematoxylin tsis tshua muaj siab ntev - lub sij hawm staining txoj kev muaj peev xwm qhia tshwj xeeb uas qhia cov cytoplasm thiab luminal qauv ntawm cov kua tsib duct epithelium yam tsis tau kom meej staining lub nucleus, pab pathologists qhia meej meej cov kev kho mob me me xws li cov kab mob me me hauv daim siab bile duct.
Thiab muab kev txhawb nqa tseem ceeb morphological rau kev kuaj mob pathological ntawm autoimmune daim siab kab mob. Hauv qhov xwm txheej ntawm staining decalcified seem ntawm cov pob txha cov ntaub so ntswg, hematoxylin tuaj yeem zam qhov tsis muaj zog acidic ib puag ncig ntawm residual decalcifying tov zoo. Ntev npaum li nws tau txais kev kho mob cyanation tsim nyog, nws tseem tuaj yeem ua kom pom tseeb stain lub nuclei ntawm cov pob txha hlwb thiab cov ciam teb ntawm cov pob txha trabeculae, tsis muaj teeb meem ntawm kev ua tiav ntawm cov xim nyob rau hauv acidic ib puag ncig zoo li ntau cov khoom siv hluavtaws dyes. Nws yog qhov txhim khu kev qha tshaj plaws nuclear staining reagent nyob rau hauv kev kuaj mob ntawm cov pob txha hlav.
Nyob rau hauv lub scene ntawm acid ceev staining thiab rov staining ntawm granulomatous lesions, tom qab pom liab tubercle bacillus los ntawm acid ceev staining, dilute.hematoxylin hmoovyog siv rau lub teeb rov staining, uas tuaj yeem ua rau cov nucleus nyob rau hauv keeb kwm yav dhau tshwm xim xiav xiav, thiab kev txheeb xyuas ntawm tubercle bacillus hauv lub teeb xiav tom qab tau zoo heev, txo qhov pom kev qaug zog ntawm cov kws kho mob nrhiav tubercle bacillus nyob rau hauv lub tshuab tsom, thiab txhim kho qhov kev kuaj pom zoo ntawm tuberculosis pathology.
Daim ntawv thov ntawm immunohistochemistry thiab molecular pathology hauv nuclear counterstaining
Nyob rau hauv cov ntsiab lus ntawm molecular pathology cov tswv yim xws li immunohistochemistry staining thiab nyob rau hauv situ hybridization rau niaj hnub precision pathological kuaj mob, hematoxylin yog lub feem ntau lug siv nuclear counterstaining reagent thiab indispensable keeb kwm staining tivthaiv nyob rau hauv tag nrho cov immunohistochemical seem. Lub hom phiaj tseem ceeb ntawm immunohistochemical staining yog kom pom cov cim zoo ntawm cov hom phiaj tshwj xeeb. Feem ntau siv DAB staining system yuav ua rau lub teeb pom kev zoo tshwm xim daj daj.
Nyob rau lub sijhawm no, lub teeb stained hematoxylin yog siv los tiv thaiv lub cell nucleus, ua rau cov cell nucleus tsis zoo uas tsis qhia txog lub hom phiaj protein tshwm sim lub teeb xiav. Lub teeb liab daj daj zoo muaj ib thaj tsam meej nyob rau hauv lub teeb xiav ntawm tes nucleus keeb kwm yav dhau. Cov kws kho mob tuaj yeem txiav txim siab seb qhov teeb meem zoo nyob rau hauv lub nucleus, cytoplasm, lossis cell membrane, thiab ua kom tiav qhov kev txhais lus thiab ib nrab qhab nia ntawm cov txiaj ntsig ntawm immunohistochemical. Hauv kev txhais cov tshuaj tiv thaiv kab mob xws li PD-L1 thiab Ki-67 ntsig txog kev kho mob qog nqaij hlav, qhov zoo ntawm hematoxylin counterstaining ncaj qha txiav txim siab qhov tseeb ntawm kev suav ntawm tes zoo.
Lub teeb xiav thiab lub teeb xiav lub teeb pom kev zoo tso cai rau cov kws kho mob kom yooj yim paub qhov txawv ntawm cov qog hlwb los ntawm cov hlwb stromal, xam qhov feem ntawm cov qog nqaij hlav zoo, thiab muab cov qauv qhia meej meej rau lub hom phiaj thiab cov tshuaj tiv thaiv kab mob rau cov neeg mob qog. Nyob rau hauv qhov kev tshawb nrhiav molecular pathology scenario ntawm fluorescence nyob rau hauv situ hybridization (FISH), hematoxylin ua hauj lwm raws li lub ci teb ua ntej staining reagent, uas muaj peev xwm kom meej meej piav qhia lub cell contour ntawm cov ntaub so ntswg ua ntej fluorescence kev soj ntsuam, pab sim sai nrhiav lub hom phiaj qog cell cheeb tsam, zoo heev txhim kho cov kev soj ntsuam kev soj ntsuam ntawm FISH teeb liab efficiency.
Zam kev tshawb nrhiav tsis muaj txiaj ntsig rau lub hom phiaj ntawm cov hlwb hauv ntau lub hlwb interstitial, thiab txhim kho qhov ua tau zoo thiab qhov tseeb ntawm FISH tshawb pom. Nyob rau hauv tshwj xeeb immunoelectron microscopy qauv npaj scenarios, uranyl acetate hematoxylin ua ke staining txoj kev tuaj yeem txhim kho cov electron ceev ntawm cell membranes thiab nuclear membranes hauv ultra-thin sections, ua rau cellular ultrastructure txheej clearer nyob rau hauv electron microscopy, thiab pab cov kws tshawb fawb pathological kom pom cov kab mob hauv cov kab mob hauv zos thiab cov kab mob. hlwb.
Nws tau siv dav hauv kev tshawb fawb ultra-thin pathological hauv virology thiab neuroscience. Nyob rau hauv cov kua raws nyias- txheej cell kuaj scenario ntawm chaw kho mob cytology,hematoxylin hmoovstaining tuaj yeem nthuav qhia cov chromatin kev ntxhib los mos ntawm lub ncauj tsev menyuam exfoliated hlwb, thiab cov kws kho mob tuaj yeem txheeb xyuas cov yam ntxwv ntawm cov morphological tsis tu ncua ntawm cov ntaub so ntswg nuclear thiab tsis sib xws ntawm chromatin nyob rau hauv lub nucleus, zoo heev txhim kho qhov zoo ntawm kev kuaj pom tus kab mob qog noj ntshav hauv tsev menyuam raws li kev tshuaj ntsuam xyuas cytology thiab txo qhov kev pheej hmoo ntawm qhov tsis zoo thiab tsis pom zoo.
FAQ
Puas yog hematoxylin liab lossis ntshav liab?
Hematoxylin thiab Eosin, feem ntau hu ua H&E, yog suav tias yog ib qho kev niaj hnub stain hauv kev txheeb xyuas cov qauv morphological hauv cov ntaub so ntswg. Lub nuclei nyob rau hauv cov stain no yog stained ib tug xiav -liab xim los ntawm hematoxlyin. Eosin yog siv los ua ib lub txee rau kos cytoplasm thiab cov leeg nqaij hauv cov xim liab sib txawv.
Dab tsi yog qhov hloov pauv rau hematoxylin?
Cov hlau alum-celestine xiav kua yog ruaj khov rau ob peb lub hlis. Lub sijhawm staining ntawm 5-10 feeb feem ntau yog txaus siab rau cov ntaub so ntswg formalin. Txoj kev no tau raug pom zoo los ua ib qho kev hloov rau Hematoxylin thiab Eosin.
Lub npe hu ua hematoxylin yog dab tsi?
Raws li kev zas xim, haematoxylin tau raug hu ua palo de Campeche, logwood extract, bluewood thiab blackwood. Hauv histology, haematoxylin staining feem ntau ua raws li counterstaining nrog eosin. Thaum ua khub, cov txheej txheem staining no hu ua H&E staining thiab yog ib qho kev sib xyaw ua ke feem ntau hauv histology.
Puas yog hematoxylin zoo lossis tsis zoo?
Hematoxylin nyob rau hauv complex nrog txhuas yog cationic thiab ua raws li ib tug yooj yim dye. Vim tias nws raug nqi zoo, nws tuaj yeem khi rau qhov tsis zoo, basophilic cell Cheebtsam, xws li nucleic acids hauv lub nucleus. Cov no tau stained xiav lossis ntshav liab raws li qhov tshwm sim.
Cim npe nrov: hematoxylin hmoov cas 517-28-2, lwm tus neeg, manufacturers, hoobkas, lag luam wholesale, yuav, nqe, tej, kev muag khoom