Bicuculline yog ib qho kev koom tes feem ntau hauv ntau qhov chaw, suav nrog kev tshawb nrhiav neuroscience, pharmacology, thiab tshuaj. Raws li tus txiv neej muaj zog ntawm gamma-aminobutyric corrosive (GABA) receptors, nws tau hloov mus rau hauv cov khoom siv tsis muaj nqi rau kev tsom mus rau txoj haujlwm ntawm GABA hauv kev muaj peev xwm neuronal thiab tshawb xyuas cov khoom sib txawv ntawm cov teeb meem paj hlwb. Txawm li cas los xij, tib yam li lwm cov khoom sib txuas, qhov ruaj khov thiab lub sijhawm ntawm kev siv tau tiag tiag ntawmbicucullineyog cov ntsiab lus tseem ceeb uas yuav tsum tau xav txog, tshwj xeeb hauv kev tshawb fawb thiab chaw kho mob. Nyob rau hauv tsab xov xwm no, peb yuav dhia mus rau hauv seb nws mus phem, tshuaj xyuas nws cov khoom, amassing tej yam kev mob, thiab cov txheej txheem rau xaiv nws uprightness.
Bicuculline yog dab tsi thiab nws siv li cas?
Bicucullineyog ib qho tshwm sim tsis tu ncua alkaloid tau txais los ntawm cov nroj tsuag sib txawv, piv txwv li, cov cag ntoo ntawm Japanese cog Dicentra cucullaria. Nws yog tus neeg phem phem ntawm GABA-A receptor, uas yog qhov tseem ceeb inhibitory synapse receptor hauv focal sensory system (CNS).
Hauv kev tshawb fawb neuroscience, nws tau siv dav los ua cov khoom siv tshuaj los tshuaj xyuas txoj haujlwm ntawm GABA hauv cov kab mob sib txawv, suav nrog kev sib txuas lus sib txuas, neuronal volatility, thiab lub koom haum cov ntsiab lus. Los ntawm kev cuam tshuam cov teebmeem inhibitory ntawm GABA, nws tuaj yeem cuam tshuam kev qaug dab peg zoo li hauv cov phiaj xwm neuronal, tso cai rau cov neeg soj ntsuam tsom mus rau qhov tseem ceeb ntawm kev qaug dab peg thiab lwm yam teeb meem ntawm lub paj hlwb (Khalilov li al., 2003).
Tsis tas li ntawd, cov khoom siv tau siv hauv kev tshawb nrhiav cov qauv ntawm kev nruj, kev tsim txom, thiab kev txhim kho, raws li GABA suav tias yog ib qho tseem ceeb hauv kev tweaking cov xwm txheej no (Crestani li al., 2002; Gassmann thiab Bettler, 2012).
Dab tsi yog Cov Cai Cia Rau Bicuculline?
Ib yam li lwm cov khoom sib xyaw hluavtaws, lub zog thiab lub sijhawm ntawm kev siv tau tiag tiag ntawm cov khoom siv tshwj xeeb yog nyob ntawm qhov muaj peev xwm raug cai. Raws li cov ntaub ntawv uas tau muab los ntawm cov chaw muab kev pabcuam sib txawv, xws li Sigma-Aldrich thiab Cayman Synthetic, nws yuav tsum tau muab tso tseg nyob rau hauv qhov xwm txheej tsis txaus ntseeg kom ua raws li nws txoj kev hwm thiab lub zog.
Cov kev pom zoo khaws cia rau nws feem ntau suav nrog:
1. Kub: Nws yuav tsum tau saib xyuas ntawm qhov kub thiab txias, tsis tu ncua qee qhov chaw ntawm 2 degree thiab 8 degree (35.6 degree F thiab 46.4 degree F). Kev txais mus rau qhov kub siab dua tuaj yeem ua kom muaj kev sib koom tes ntawm kev noj nyiaj txiag thiab cuam tshuam rau qhov sib xyaw ua ke tsis zoo.
2. Lub teeb kev ruaj ntseg: Nws yog ilv rau lub teeb qhib, uas tuaj yeem ua haujlwm nrog photochemical teb thiab ua rau debasement. Nyob rau hauv txoj kev no, nws yuav tsum tau muab tso rau hauv qhov tsis pom tseeb lossis lub teeb ruaj ntseg kom txwv lub teeb qhib.
3. Kev ruaj ntseg dampness: Cov khoom yog hygroscopic, txhais tau tias nws tuaj yeem nqus dej noo los ntawm huab cua. Kev qhib rau dampness tuaj yeem ua rau hydrolysis thiab ua rau kev noj nyiaj txiag. Muaj peev xwm nyob rau hauv ib qho ruaj khov, dampness ruaj ntseg compartment yog qhia.
4. Ib puag ncig tsis muaj zog: Ob peb tus neeg muag khoom pom zoo saib xyuas nws nyob rau hauv ib puag ncig inert, zoo ib yam li nitrogen lossis argon, txwv tsis pub oxidation thiab nthuav nws lub sijhawm yooj yim.
Nws yog ib qho tseem ceeb kom ua raws li cov lus qhia muaj peev xwm muab los ntawm tus tsim khoom lossis tus neeg muab kev pabcuam kom lav qhov zoo tshaj plaws kev ruaj ntseg thiab lub zog ntawm nws.
Yuav ua li cas txiav txim siab yog tias Bicuculline tau ploj mus?
Tsis hais txog cov khoom tsim nyog raug cai,BicucullineTej zaum yuav muaj kev puas tsuaj tom qab qee lub sijhawm vim muaj cov ntsiab lus sib txawv, piv txwv li, qhib rau lub teeb, siv, lossis dampness. Yog li, nws yog ib qho tseem ceeb rau kev ntsuam xyuas qhov kev hwm thiab lub zog ntawm cov khoom ua ntej koom nrog hauv kev sim lossis kev siv tshuaj kho mob. Nov yog ob peb txoj hauv kev uas tuaj yeem siv los txiav txim siab seb nws puas tau hloov qaub:
1. Kev tshuaj xyuas qhov muag: Kev tshuaj xyuas qhov muag ncaj qha tuaj yeem muab cov ntaub ntawv tseem ceeb txog qhov tseeb ntawm nws. Ib qho kev nce qib hauv ntau yam, zoo li cov khoom me me los yog txhawb nqa, los yog qhov qhia tias dampness los yog crystallization yuav qhia kev noj nyiaj txiag lossis tainting.
2. Cov tswv yim zoo: Cov tswv yim sib txawv tuaj yeem siv los soj ntsuam qhov tsis zoo, lub zog, thiab kev ncaj ncees ntawm cov khoom. Cov txheej txheem no suav nrog:
a. Cov neeg tseem ceeb execution fluid chromatography (HPLC): HPLC yog ib lub tswv yim dav dav rau kev sib cais, lees paub, thiab ntsuas cov khoom sib txuas. Nws tuaj yeem txheeb xyuas cov pa phem, cov khoom tsis zoo, thiab ntsuas qhov tsis zoo ntawm cov khoom kuaj.
b. Mass spectrometry (MS): MS tuaj yeem ua ke nrog HPLC lossis siv dawb los lees paub thiab piav qhia txog kev tsim kho ntawm nws thiab txhua yam khoom muaj peev xwm debasement.
c. Atomic attractive reverberation (NMR) spectroscopy: NMR tuaj yeem muab cov ntaub ntawv qhia txog cov khoom siv hluavtaws thiab tsis zoo ntawm cov khoom kuaj, tso cai rau kev lees paub ntawm cov pa phem lossis kev hloov pauv thawj zaug.
3. Kev soj ntsuam ua haujlwm: Qee qhov xwm txheej, tshwj xeeb tshaj yog nyob rau hauv kev tshawb fawb chaw, kev soj ntsuam ua haujlwm yuav raug ua haujlwm los soj ntsuam cov dej num lom neeg thiab lub zog ntawm nws. Cov kev ntsuas no feem ntau suav nrog ntsuas qhov cuam tshuam ntawm nws ntawm GABA-A receptor muaj nuj nqi hauv cov qauv ntawm tes lossis cov ntaub so ntswg.
Yog tias ib qho ntawm cov txheej txheem no ua rau pom qhov cuam tshuam loj heev, muaj kuab paug, lossis tsis muaj zog, cov khoom kuaj yuav tsum tau muab pov tseg thiab hloov nrog ib pawg tshiab los lav cov txiaj ntsig zoo thiab rov tsim dua tshiab.
Nws txhais tau hais tias muaj ntau yam uas yuav tsum nco ntsoov tias cov txheej txheem tshwj xeeb thiab kev lees paub ntsuas rau kev txiav txim siab qhov kev ntseeg siab ntawm nws yuav hloov pauv raws li qhov xav tau ntawm daim ntawv thov thiab cov thawj coj ua ntej tsim. Cov chaw tshawb fawb, cov koom haum tshuaj, thiab chaw kho mob tej zaum yuav muaj ntau yam kev qhia thiab cov lus pom zoo rau kev tswj hwm thiab kev pom zoo.
Txhua yam, thaum nws yog ib qho tseem ceeb nrog ntau yam kev siv, nws lub zog thiab lub sij hawm ncua sij hawm ntawm kev siv tau yog qhov tseem ceeb ntawm kev xav. Cov kev muaj peev xwm raug cai, raws li tau hais los ntawm tus tsim khoom lossis tus neeg muab kev pabcuam, yog qhov tseem ceeb kom ua raws li kev ntseeg siab thiab lub zog ntawm nws. Kev kuaj xyuas thiab kev soj ntsuam kev cai siv cov txheej txheem sib txawv thiab cov txheej txheem siv tau tuaj yeem pab txiav txim siab seb nws puas tau tig qaub, lav qhov kev cia siab thiab rov tsim dua cov txiaj ntsig hauv kev tshawb fawb thiab chaw kho mob.
Cov ntaub ntawv:
1. Cayman Tshuaj. (nd). Cov ntaub ntawv ntawm cov khoom siv bicuculline.
2. Crestani, F., Keist, R., Fritschy, JM, Benke, D., Vogt, K., Prut, L., ... & Rudolph, U. (2002). Trace ntshai txias muaj xws li hippocampal 5 GABA(A) receptors. Cov txheej txheem ntawm National Academy of Sciences, 99(13), 8980-8985.
3. Gassmann, M., & Bettler, B. (2012). Kev tswj hwm ntawm neuronal GABA (B) receptor ua haujlwm los ntawm subunit muaj pes tsawg leeg. Nature Reviews Neuroscience, 13(6), 380-394.
4. Khalilov, I., Holmes, GL, & Ben-Ari, Y. (2003). Nyob rau hauv vitro tsim ntawm ib tug thib ob epileptogenic daim iav tsom los ntawm interhippocampal propagation ntawm qaug dab peg. Nature Neuroscience, 6(10), 1079-1085.
5. Sigma-Aldrich. (nd). Cov ntaub ntawv ntawm cov khoom siv bicuculline.