Ntshiab inositol hmoov, tseem hu ua cyclohexanol. CAS 87-89-8, hmoov dawb. Cov uas tsis muaj dej crystalline tsis yog hygroscopic dawb crystalline hmoov. Crystals uas muaj bimolecular crystalline dej yog weathered crystals nrog lub cev qhuav dej kub ntawm 100 degree. Tsis muaj ntxhiab thiab qab zib hauv saj. Nyob ruaj khov hauv huab cua. Soluble nyob rau hauv dej, insoluble nyob rau hauv anhydrous ethanol, ether, chloroform, thiab nruab nrab nyob rau hauv aqueous tov. Dav faib rau tsiaj thiab nroj tsuag, nws yog ib qho kev loj hlob ntawm cov tsiaj thiab cov kab mob. Nws yog thawj zaug cais tawm ntawm myocardium thiab daim siab. Muaj ntau yam cis thiab trans isomers ntawm inositol nyob rau hauv cov xwm, thiab cov isomers tshwm sim yog cis 1,2,3,5-trans 4,6-cyclohexanol. Nws lub luag haujlwm tseem ceeb yog koom nrog cov txheej txheem xws li xov tooj ntawm tes, lipid synthesis, thiab insulin ua haujlwm, uas yog qhov tseem ceeb rau tib neeg kev noj qab haus huv. Piv txwv li, inositol yog qhov tseem ceeb tshwj xeeb rau kev tswj hwm cov tshuaj insulin li qub thiab tuaj yeem pab tswj cov ntshav qab zib. Rau cov neeg mob ntshav qab zib mellitus, kev siv tshuaj inositol tuaj yeem ua tau zoo. Qhov zoo tshaj plaws inositol hmoov yog lub ntuj tsim muaj nyob rau hauv tib neeg lub hlwb, teej tug mus rau vitamin B pawg thiab kuj hu ua vitamin B8. Nws plays lub luag haujlwm tseem ceeb hauv ntau cov txheej txheem physiological ntawm tib neeg lub cev.
(Cov khoom txuas: https://www.bloomtechz.com/synthetic-chemical/organic-materials/pure-inositol-powder-cas-87-89-8.html )
Raws li cov tshuaj nquag siv, nrog rau kev siv ntau ntxiv ntawm inositol, cov kws tshawb fawb tau xav txog nws txoj kev sib xyaw ua ke, vam tias yuav tshawb nrhiav txoj hauv kev zoo dua los ua kom cov tshuaj muaj txiaj ntsig zoo dua. Tam sim no, muaj ob txoj hauv kev: chemical method thiab enzymatic method. Cov lus qhia tshwj xeeb yog raws li hauv qab no.
Txoj kev tshuaj:
Inositol yog cyclic polyol nrog cov qauv tshuaj zoo ib yam li qabzib. Cov nram qab no yog cov ncauj lus kom ntxaws cov kauj ruam thiab tshuaj sib npaug rau cov tshuaj synthesis ntawm inositol:
Lub synthesis ntawm inositol feem ntau yog ua los ntawm reacting tus coj halogenated cyclohexane nrog ib tug reagent nyob rau hauv alkaline tej yam kev mob. Formatting reagents yog tsim los ntawm cov tshuaj tiv thaiv ntawm metallic sodium nrog anhydrous ethanol. Cov tshuaj tiv thaiv kab mob sib npaug rau cov kauj ruam no yog raws li hauv qab no:
(CH3)2CHOH + NaH → (CH3)2CHAW + H2
Tom ntej no, cov tsim tawm hom reagent reacts nrog halogenated cyclohexane los tsim inositol. Cov tshuaj tiv thaiv kab mob sib npaug rau cov kauj ruam no yog raws li hauv qab no:
(CH3)2CH-ONa + CH2Cl2→ (CH3)2CH-O-CH2-CH(Cl)-CH2OH + NaCl
Cov inositol generated tuaj yeem ua kom huv los ntawm kev sib cais thiab kev ua kom huv. Cov kauj ruam no suav nrog kev sib cais cov inositol tsim hauv dej, acidification nrog dilute hydrochloric acid rau pH =4, thiab evaporation rau concentration. Tom qab ntawd, cov tshuaj concentrated yog alkalized nrog sodium hydroxide rau pH =11, thiab tom qab ntawd diluted nrog dej rau 1/5 ntawm qhov qub ntim. Thaum kawg, cov tshuaj tau raug lim thiab acidified nrog dilute hydrochloric acid rau pH =7, ua rau cov khoom ntawm inositol.
Cov saum toj no yog cov ncauj lus kom ntxaws cov kauj ruam thiab tshuaj sib npaug rau cov tshuaj synthesis ntawm inositol. Nws yuav tsum tau muab sau tseg tias qhov no yog ib qho kev ntsuas ntsuas ntsuas, thiab cov txheej txheem sib txawv thiab cov khoom siv raw tuaj yeem siv rau hauv kev tsim khoom tiag tiag. Lub caij no, cov tshuaj lom neeg muaj feem cuam tshuam nrog rau txoj kev no yuav ua rau muaj kev pheej hmoo thiab yuav tsum tau ua haujlwm raws li kev taw qhia ntawm lub chaw kuaj mob.
Qhov zoo ntawm cov tshuaj synthesis ntawm inositol feem ntau tshwm sim hauv cov hauv qab no:
1. Cov txheej txheem tshuaj muaj peev xwm ua kom cov khoom sib txuas nrog cov txheej txheem zoo, xws li inositol, thiab cov txheej txheem synthesis yog cov paub tab, ua rau muaj kev ua haujlwm siab.
2. Cov tshuaj tiv thaiv kab mob thiab tsis muaj tshuaj lom neeg txoj kev tuaj yeem raug tswj kom ua tiav cov khoom zoo thiab ruaj khov.
3. Cov txheej txheem tshuaj muaj kev siv dav hauv kev sib xyaw ua ke ntawm ntau hom kev sib txuas, thiab tuaj yeem siv los ua ke ntau hom organic tebchaw, xws li cawv, aldehydes, ketones, thiab lwm yam.
4. Cov txheej txheem tshuaj lom neeg kuj muaj qee yam kev siv hauv kev sib xyaw ntawm cov khoom ntuj tsim, uas tuaj yeem siv los rho tawm thiab ua kom cov tshuaj ntuj, tshuaj tsw qab, thiab lwm yam.
Qhov tsis zoo yog feem ntau tshwm sim hauv cov hauv qab no:
1. Kev siv tshuaj lom neeg feem ntau yuav tsum tau siv ntau cov kuab tshuaj organic thiab cov tshuaj reagents, uas tsis yog tsuas yog ua phem rau ib puag ncig xwb tab sis kuj yuav ua rau muaj kev hem thawj rau kev noj qab haus huv ntawm cov neeg ua haujlwm.
2. Cov kauj ruam ntawm cov tshuaj synthesis muaj ntau heev thiab complex, yuav tsum tau rov sib cais, purification, thiab refinement, uas ua rau tsis tshua muaj ntau lawm efficiency.
3. Kev siv tshuaj lom neeg feem ntau yuav tsum muaj qhov kub thiab txias siab, uas ua rau cov nqi ntau lawm thiab muaj kev nyab xeeb.
4. Cov khoom ntawm cov tshuaj synthesis tej zaum yuav muaj cov khoom siv raw uas tsis muaj kev cuam tshuam, cov khoom nruab nrab, thiab cov khoom siv los ntawm cov khoom, uas yuav tsum tau sib cais thiab ua kom huv ntxiv, ua rau muaj kev nyuaj siab ntau ntxiv thiab tus nqi.
5. Cov tshuaj tiv thaiv kab mob thiab cov kev tsis sib haum xeeb ntawm cov tshuaj synthesis yuav tsum tau tswj kom raug, txwv tsis pub nws yuav ua rau cov khoom tsis ruaj tsis khov lossis kev tsim khoom raug mob.
Yog li ntawd, txawm hais tias cov txheej txheem tshuaj muaj peev xwm ua ke inositol, nyob rau hauv niaj hnub kev lag luam ntau lawm, tib neeg yuav siv ntau dua environmentally friendly, npaum, thiab kev nyab xeeb txoj kev xws li enzymatic txoj kev los tsim cov tebchaw xws li inositol.
Txoj kev Enzymatic:
Lub enzymatic synthesis ntawm inositol yog ib tug kuj ntsuab thiab npaum txoj kev uas siv tshwj xeeb enzymes catalyze substrate tshwm sim los tsim inositol. Cov nram qab no yog cov ncauj lus kom ntxaws cov kauj ruam thiab tshuaj sib npaug rau enzymatic synthesis ntawm inositol:
1. Kev npaj cov substrates: Cyclohexanol (raws li cov khoom pib) thiab qabzib -6-phosphate yog substrates rau enzymatic cov tshuaj tiv thaiv. Cov substrates no feem ntau yuav tsum tau ua kom huv thiab huv ua ntej cov tshuaj tiv thaiv kom ntseeg tau tias lawv cov purity thiab cov ntsiab lus.
2. Kev xaiv Enzyme: Lub enzymatic synthesis ntawm inositol yuav tsum tau xaiv cov enzymes tsim nyog, xws li inositol phosphate synthase (MIPS). Cov enzyme no tuaj yeem ua rau cov tshuaj tiv thaiv substrate los tsim inositol 6-phosphate.
3. Cov kev mob tshwm sim: Cov tshuaj tiv thaiv feem ntau yog ua raws li qhov mob me me, xws li qis qis thiab ze li qhov nruab nrab pH. Qhov no tuaj yeem ua kom muaj kev ua haujlwm thiab kev ruaj ntseg ntawm cov enzyme thiab txo qhov tshwm sim ntawm kev tshwm sim sab.
4. Cov txheej txheem tshuaj tiv thaiv: Hauv cov tshuaj tiv thaiv enzymatic, cyclohexanol yog siv los ua cov khoom pib los teb cov piam thaj -6-phosphate raws li kev ua ntawm MIPS los tsim inositol-6-phosphate. Kev sib npaug tshuaj rau cov kauj ruam no yog raws li nram no:
C6H11OH + C6H13O9P + C3H7ClN2O2S + Mg 2+ → C6H13O6P + C10H15N5O10P2 + C3H7N
Ntawm lawv, C6H11OH sawv cev rau cyclohexanol, G6P sawv cev rau qabzib -6-phosphate, ATP sawv cev rau adenosine triphosphate, Mg2+ sawv cev rau magnesium ion, C6H13O6P sawv cev inositol-6-phosphate, ADP sawv cev rau adenosine thiab diphosphate. inorganic pyrophosphate.
5. Cov khoom sib cais thiab kev ua kom huv: Cov inositol generated 6-phosphate yuav tsum tau muab cais thiab purified kom tau cov ntshiab inositol. Cov kauj ruam no feem ntau suav nrog cov txheej txheem xws li kev rho tawm, crystallization, thiab recrystallization kom ntseeg tau tias kev tsim cov high-purity inositol.
6. Tom qab hloov dua siab tshiab: Rau qee yam kev siv, inositol 6-phosphate tej zaum yuav xav tau tshuaj hloov dua siab tshiab los tsim lwm cov tebchaw. Piv txwv li, los ntawm kev ua ntawm phosphatase, inositol 6-phosphate tuaj yeem tshem tawm cov phosphate pawg thiab tsim inositol.
Zuag qhia tag nrho, enzymatic synthesis ntawm inositol yog ib txoj hauv kev zoo uas siv cov enzymes tshwj xeeb los catalyze substrate tshua kom tau txais cov khoom lag luam. Qhov zoo ntawm txoj kev no muaj xws li kev xaiv siab thiab tsis tshua muaj tshwm sim ntawm sab kev xav. Txawm li cas los xij, tseem muaj qee qhov kev sib tw hauv enzymatic synthesis ntawm inositol, xws li cov nqi substrate siab thiab tsis txaus enzyme stability. Txhawm rau daws cov teeb meem no, cov kws tshawb fawb niaj hnub tshawb nrhiav cov thev naus laus zis tshiab thiab cov txheej txheem los ua kom zoo dua cov txheej txheem no.
Qhov zoo ntawm enzymatic synthesis ntawm inositol feem ntau suav nrog:
1. High tshwj xeeb: Cov tshuaj tiv thaiv Enzymatic muaj qhov tshwj xeeb siab, uas tuaj yeem ua kom ntseeg tau qhov tseeb ntawm cov tshuaj tiv thaiv thiab cov khoom purity siab.
2. Cov mob me me: Cov tshuaj tiv thaiv Enzymatic feem ntau yog ua raws li qhov mob me me thiab tsis tas yuav muaj cov xwm txheej hnyav xws li kub thiab siab, ua rau lawv muaj kev nyab xeeb thiab zoo ib puag ncig.
3. Cov tshuaj tiv thaiv ceev ceev: Cov tshuaj tiv thaiv Enzymatic muaj cov tshuaj tiv thaiv ceev ceev, uas tuaj yeem ua rau luv luv lub voj voog thiab txhim kho kev tsim khoom.
4. Kev txuag hluav taws xob: Cov tshuaj tiv thaiv Enzymatic tuaj yeem nqa tawm ntawm chav tsev kub thiab siab, tsis tas yuav tsum muaj ntau cov organic solvents thiab tshuaj reagents, yog li txuag lub zog thiab cov peev txheej.
Enzymatic synthesis ntawm inositol kuj muaj qee qhov tsis zoo:
1. Lub hauv paus ntawm enzymes yog txwv: Cov tshuaj tiv thaiv enzymatic yuav tsum tau siv cov enzymes tshwj xeeb ua catalysts, thiab qhov chaw ntawm cov enzymes feem ntau txwv thiab tej zaum yuav xav tau kev cog qoob loo thiab rho tawm.
2. Tus nqi ntawm cov enzymes kuj tseem siab: Vim muaj cov enzymes tsawg, lawv cov nqi feem ntau yog siab, uas yuav ua rau cov nqi ntau lawm.
3. Kev cuam tshuam ib puag ncig: Txawm hais tias cov tshuaj tiv thaiv enzymatic tau ua nyob rau hauv cov mob me me, qee cov organic solvents thiab tshuaj reagents tseem yuav tsum tau, uas tej zaum yuav muaj qee yam cuam tshuam rau ib puag ncig.
Enzymatic synthesis ntawm inositol muaj qhov zoo xws li qhov tshwj xeeb thiab cov tshuaj tiv thaiv me me, tab sis kuj tseem muaj qee qhov tsis zoo xws li txwv cov enzymes, tus nqi siab, thiab kev cuam tshuam ib puag ncig. Hauv kev siv tswv yim, nws yuav tsum ua tib zoo xav txog nws qhov zoo thiab qhov tsis zoo, thiab xaiv ib txoj hauv kev tsim nyog los tsim cov tebchaw xws li inositol.