Kisspeptin(link:https://www.bloomtechz.com/synthetic-chemical/peptide/kisspeptin-powder-cas-1145998-81-7.html) yog ib qho me me molecule peptide tsim los ntawm 54 amino acid residues. Nws cov qauv molecular yog C26H45N7O6S, nrog rau qhov hnyav molecular ntawm 5477.73 Daltons. Cov amino acid ib ntus ntawm kisspeptin peptide tau txais txiaj ntsig zoo hauv ntau hom, uas txhais tau hais tias nws cov qauv tseem ceeb tsis hloov pauv hauv ntau yam kab mob. Nws cov protein ua ntej dhau los ua ntau yam kev hloov kho thiab cov txheej txheem sib txuas thaum lub sijhawm txhais lus, thaum kawg tsim Kisspeptin paub tab. Cov qauv muaj xya amino acid residues - Helix, uas yog antiparallel rau ib tug qauv tsim ntawm plaub amino acid residues - Folding saw txuas. Cov qauv no tso cai rau kev khi rau G protein ua ke receptor (GPR54) thiab ua kom nws txoj kev taw qhia.
Kisspeptin tsis tsuas yog tswj cov poj niam kev ua me nyuam hauv plab hauv nruab nrab hypothalamus thiab pituitary caj pas, tab sis kuj ua lub luag haujlwm hauv lub zes qe menyuam hauv zos. Lub zos kisspeptin peptide nyob rau hauv lub zes qe menyuam tsis yog tsuas yog koom nrog hauv kev tswj kev ua haujlwm ntawm oocytes, granulosa hlwb, thiab corpus luteum, tab sis kuj cuam tshuam los ntawm gonadotropins, photoperiod, sympathetic paj hlwb, thiab metabolism.
Kisspeptin yog ib qho tseem ceeb neuropeptide uas tswj cov poj niam kev ua haujlwm ntawm kev ua me nyuam, qhia nyob rau hauv lub hypothalamus, pituitary, thiab zes qe menyuam, thiab muaj kev tswj cov teebmeem ntawm ntau theem ntawm HPOA. Kisspeptin yog ib yam khoom ntawm melanoma cell inhibitory gene KiSS-1, thiab yog ib qho ntuj peptide cais los ntawm Kotani li al. xyoo 2001 uas nthuav tawm agonistic ua haujlwm tawm tsam menyuam ntsuag G protein txuas nrog receptor GPR54 hauv tib neeg placenta.
Kisspeptin, tseem hu ua Kiss1 peptide lossis RFRP-1 peptide, yog ib qho neuropeptide pom hauv tib neeg lub cev.
1. Tshuaj muaj pes tsawg leeg: Kisspeptin yog ib tug me me molecule peptide muaj li ntawm 54 amino acid residues, nrog ib tug molecular mis ntawm C26H45N7O6S thiab ib tug molecular hnyav ntawm 5477.73 Daltons. Nws cov qauv suav nrog hydrophilic N-terminal uas muaj cuaj amino acids thiab hydrophobic C-terminal uas muaj plaub amino acids.
2. Cov qauv tshuaj: Cov amino acid ib ntus ntawm Kisspeptin tau txais txiaj ntsig zoo hauv ntau hom, uas txhais tau hais tias nws cov qauv tseem ceeb tsis hloov pauv hauv ntau yam kab mob. Nws cov qauv suav nrog qhov ntev N-lub davhlau ya nyob twg thiab luv dua C-terminal. Tsis tas li ntawd, Kisspeptin tseem muaj cysteine (Cys) residue, uas ua lub luag haujlwm tseem ceeb hauv cov txheej txheem disulfide ntawm peptides.
3. Cov qauv thib ob: Cov qauv thib ob ntawm Kisspeptin feem ntau yog tsim los ntawm - Muaj cov kauv thiab random curls. Ntawm lawv, random curling occupies feem ntau ntawm peptide, thaum - Lub helix tsuas yog tso nyiaj rau 20% ntawm tag nrho cov peptide. Cov qauv thib ob no ua rau Kisspeptin khi rau G protein txuas nrog receptor (GPR54) thiab qhib nws txoj kev taw qhia.
4. Tertiary qauv: Cov qauv tertiary ntawm Kisspeptin tau txiav txim siab los ntawm X-ray crystal diffraction thiab nuclear magnetic resonance (NMR) cov tswv yim. Nws cov qauv muaj xya amino acid residues - Helix, uas yog antiparallel rau ib tug qauv tsim ntawm plaub amino acid residues - Folding saw txuas. Cov qauv no ua rau Kisspeptin khi rau GPR54 thiab qhib nws txoj kev taw qhia.
5. Stereoscopic configuration: Kisspeptin yog ib qho S-configuration nrog ib tug trans cycled stereoconfiguration. Qhov kev teeb tsa peb sab no yog qhov tseem ceeb rau kev khi ntawm Kisspeptin rau GPR54 thiab ua kom nws txoj kev taw qhia.
6. Kev hloov kho thiab kev sib txuas: Kisspeptin precursor tau dhau los ua cov txheej txheem hloov kho thiab cov txheej txheem splicing thaum lub sijhawm txhais lus, thaum kawg ua rau Kisspeptin paub tab. Cov txheej txheem no suav nrog proline hydroxylation, glycine dehydrogenation, thiab disulfide daim ntawv cog lus. Cov kev hloov kho thiab cov txheej txheem splicing yog qhov tseem ceeb rau kev ua haujlwm lom neeg ntawm Kisspeptin.
7. Kev ruaj ntseg: Kisspeptin muaj qee qhov kev ruaj ntseg hauv lub cev. Nws muaj qee yam tsis kam rau degrading enzymes xws li acid, alkali, trypsin, thiab pepsin. Txawm li cas los xij, nyob rau hauv tej yam kev mob, Kisspeptin kuj yuav degraded, tshwj xeeb tshaj yog nyob rau hauv high kub, muaj zog acid los yog alkali ib puag ncig.
8. Chemical synthesis thiab recombination: Kisspeptin tuaj yeem tau los ntawm kev sib xyaw tshuaj los yog cov txheej txheem rov ua dua. Kev siv tshuaj lom neeg feem ntau muaj ntau yam tshuaj lom neeg lom zem, nrog rau kev sib xyaw ua ke, kev tiv thaiv, desalination, thiab lwm yam. Txoj kev rov ua dua tshiab, ntawm qhov tod tes, siv cov txheej txheem kev tsim kho caj ces los nthuav qhia cov protein ua ntej ntawm Kisspeptin hauv cov kab mob xws li Escherichia coli lossis poov xab, thiab tom qab ntawd ua tiav tom qab tau txais Kisspeptin paub tab.
9. Kev kuaj xyuas thiab kev tshuaj xyuas: Cov ntsiab lus ntawm Kisspeptin hauv cov kab mob muaj tsawg heev, yog li kev ntsuas kev xav tau xav tau los kuaj xyuas thiab tshuaj xyuas. Cov txheej txheem no suav nrog radioimmunoassay (RIA), enzyme-linked immunosorbent assay (ELISA), huab hwm coj spectrometry (MS), thiab lwm yam. Ntawm lawv, ELISA yog ib qho ntawm feem ntau siv txoj kev tshawb nrhiav, uas tuaj yeem tshawb xyuas cov ntsiab lus ntawm Kisspeptin thiab nws cov kev hloov hauv lub cev.
Kisspeptin, tseem hu ua Kiss1 peptide lossis RFRP-1 peptide, yog ib qho neuropeptide thawj zaug cais tawm ntawm tib neeg lub cev hauv xyoo 2001. Nws feem ntau koom nrog kev tswj hwm ntawm poj niam cev xeeb tub thiab txhawb kev tso tawm ntawm GnRH los ntawm kev khi rau G protein. ua ke receptor GPR54, yog li ua lub luag haujlwm hauv cov haujlwm tom ntej ntawm kev ua me nyuam axis. Nyob rau hauv xyoo tas los no, nrog rau kev tshawb fawb tob ntawm Kisspeptin, nws tau qhia nyob rau hauv lub hauv paus hypothalamus, pituitary, thiab zes qe menyuam, tswj ntau yam kev sib txuas ntawm HPOA, thiab tuaj yeem ua lub luag haujlwm tseem ceeb hauv cov kab mob xws li qog. Yog li ntawd, txoj kev loj hlob zeem muag ntawm Kisspeptin yog tsim nyog tos ntsoov rau.
Txawm li cas los xij, nws yuav tsum tau taw qhia tias kev tshawb fawb ntxiv thiab kev sim tshuaj ntsuam xyuas yuav tsum tau txiav txim siab seb Kisspeptin puas tuaj yeem ua lub hom phiaj tshuaj rau kev kho cov poj niam cov kab mob ntsig txog kev sib deev. Tsis tas li ntawd, kev tshawb fawb ntxiv yog xav tau los txiav txim seb Kisspeptin puas muaj peev xwm siv tau los kho lwm yam kab mob xws li qog. Hauv cov ntsiab lus, Kisspeptin ua lub luag haujlwm tseem ceeb raws li cov neuropeptide hauv kev tswj hwm ntawm poj niam cev xeeb tub, thiab nws txoj kev loj hlob yuav tsim nyog tos ntsoov rau. Tab sis kev tshawb fawb ntxiv thiab kev sim tshuaj ntsuam xyuas yog xav tau los txheeb xyuas nws cov peev xwm kho tau. Muaj kisspeptins muaj muag thaum pib ntawm Shaanxi. Yog tias koj xav tau lawv, koj tuaj yeem xa email rau kev sab laj.