Shaanxi BLOOM Tech Co., Ltd. yog ib qho ntawm cov tuam ntxhab thiab cov muag khoom ntawm itpp hmoov hauv Suav teb. Txais tos rau cov lag luam wholesale bulk zoo itpp hmoov muag ntawm no los ntawm peb lub hoobkas. Kev pabcuam zoo thiab tus nqi tsim nyog muaj.
ITPP hmoov(Inositol Trispyrophosphate) yog ib qho kev sim tshuaj uas feem ntau tshawb xyuas rau nws lub peev xwm los tswj cov pa oxygen tawm ntawm hemoglobin. Nws ua haujlwm los ntawm kev txo qis cov pa oxygen-binding affinity ntawm hemoglobin, ua kom yooj yim dua cov pa oxygen los ntawm cov hlab ntsha mus rau cov ntaub so ntswg uas tsis muaj oxygen, yog li txhim kho cov ntaub so ntswg oxygenation nyob rau hauv cov mob hypoxic. Cov txheej txheem no ua rau nws muaj peev xwm ua tau zoo hauv thaj chaw xws li kev txhim kho lub cev, kev txhawb nqa lub plawv, thiab kev kho mob ntawm ischemia ntsig txog mob. Tam sim no, ITPP tseem nyob rau hauv kev tshawb fawb txog kev kho mob thiab thaum ntxov thiab tsis tau txais kev tso cai ua lag luam los ntawm cov koom haum tswj hwm suav nrog FDA thiab EMA.
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PIB COA
Molecular Structure thiab Chemical Composition
Cov mis mos molecular ntawm ITPP (Myo-Inositol Trispyrophosphate) yog C₆H₆Na₆O₂₁P₆, thiab nws lub npe Suav yog cyclohexahexanol cyclo1,2:3,4:5,6-tris (P,P'sphoodium) ntsev Nws cov qauv tseem ceeb muaj peb ntu:
Cyclhexahexanol (inositol):Raws li cov pob txha yooj yim, nws muab rau 6 pawg hydroxyl (-OH) ua qhov chaw sib txuas rau pawg phosphate.
Tripyrophosphate pawg:Txhua pawg hydroxyl yog hloov los ntawm ib pawg pyrophosphate (P₂O₇⁴⁻), ua peb p-O-c bonds, uas tso cai rau cov molecule nrog cov nqi tsis zoo thiab cov dej solubility.
Lub cev muaj zog
Qhov tsos thiab daim ntawv:ITPP hmoovyog ib qho dawb los tawm- cov hmoov dawb uas tsis pom muaj impurities, ua tau raws li cov qauv tshuaj qib.
Solubility:Dej solubility: me ntsis soluble nyob rau hauv dej (kev puas tsuaj los ntawm ultrasound yog yuav tsum tau), thiab tom qab dissolution, nws tshwm sim raws li ib tug pob tshab los yog me ntsis turbid tov.
Organic solvents: Insoluble nyob rau hauv non{0}}polar solvents xws li ethanol thiab ether, tab sis miscible nrog ib co polar organic solvents (xws li DMSO).
Kev ruaj ntseg:Hygroscopicity: Nws muaj zog hygroscopicity thiab yuav tsum tau muab cia rau hauv qhuav thiab lub teeb - pov thawj tej yam kev mob (xws li khov cia ntawm -20℃).
Thermal stability: Melting point> 270℃(decomposition), qhia tias nws cov qauv ruaj khov ntawm qhov kub thiab txias thiab haum rau kev lag luam synthesis.
Chemical stability: ruaj khov nyob rau hauv lub pH ntau ntawm 5 mus rau 9. Acid los yog alkaline tej yam kev mob yuav ua rau hydrolysis ntawm phosphate pawg.
Chemical Properties thiab Reactivity
Acid{0}}cov khoom siv hauv paus
ITPP yog ib qho acidic ntsev (pKa≈0.3), uas tag nrho dissociates hauv dej rau hauv hexasodium ions thiab inositol pyrophosphate anions.
Nws tuaj yeem hnov mob nrog cov kua qaub muaj zog (xws li HCl) los tsim cov kua qaub dawb, tab sis cov solubility ntawm cov kua qaub dawb yog qhov tsawg heev thiab lawv nquag los nag.
Phosphate pawg tshuaj tiv thaiv
Hydrolysis cov tshuaj tiv thaiv: Nyob rau hauv acidic los yog alkaline tej yam kev mob, cov pyrophosphate pawg yuav maj mam hydrolyze rau hauv monophosphoric acid los yog inorganic phosphoric acid, uas ua rau poob ntawm kev ua si.
REDOX stability
ITPP molecule tsis muaj cov pab pawg oxidized yooj yim (xws li unsaturated bonds thiab phenolic hydroxyl pawg), thiab ruaj khov rau oxygen ntawm qhov kub thiab txias.
Nyob rau hauv lub xub ntiag ntawm oxidants muaj zog (xws li hydrogen peroxide), oxidation ntawm phosphate pawg tej zaum yuav tshwm sim, tab sis qhov no yog tsis tshua muaj nyob rau hauv kev siv tswv yim.
Kev sib raug zoo ntawm Cov Qauv thiab Kev Ua Haujlwm
Lub luag haujlwm tseem ceeb ntawm ITPP yog los ntawm nws lub peev xwm los tswj hemoglobin allosteria:
Allosteric nyhuv
ITPP txo qhov kev sib raug zoo ntawm Hb rau oxygen los ntawm tsis yog -covalently khi rau lub subunit ntawm hemoglobin (Hb) (cov pa oxygen dissociation nkhaus hloov mus rau sab xis), txhawb kev tso tawm cov pa oxygen hauv cov ntaub so ntswg hypoxic.
Lub hauv paus tsim
Kev faib tawm tsis zoo:Lub zog tsis zoo ntawm pawg pyrophosphate cuam tshuam nrog cov cheeb tsam zoo ntawm Hb nto kom ruaj khov rau allosteric conformation.
Steric hindrance:Cov qauv nruj ntawm cov pob txha cyclohexanol txwv tsis pub txav mus los ntawm Hb subunit, ntxiv tswj cov pa oxygen-binding peev.Chemical Considerations in Applications
Synthesis txheej txheem
Feem ntau, inositol yog siv los ua cov khoom siv raw, thiab cov pyrophosphate pawg tau qhia los ntawm ntau -cov kauj ruam phosphorylation cov tshuaj tiv thaiv, thiab thaum kawg cov ntsev yog purified.Cov kauj ruam tseem ceeb suav nrog kev xaiv cov phosphorylation reagents thiab kev tswj cov tshuaj tiv thaiv kom tsis txhob tsim los ntawm - khoom.
Kev tswj kom zoo
Purity: Cov ntsiab lus tseem ceeb tshaj plaws los ntawm HPLC yog ntau dua lossis sib npaug li 99%, thiab tag nrho cov impurities tsawg dua lossis sib npaug li 0.5%.
Cov hlau hnyav thiab cov kab mob: Ua raws li cov qauv ntawm Suav Pharmacopoeia lossis USP (xws li cov hmoov txhuas tsawg dua lossis sib npaug rau 0.1ppm, tag nrho cov kab mob suav tsawg dua lossis sib npaug li 1000CFU / g).
Kev khaws cia thiab kev thauj mus los
Nws yuav tsum tau muab cia rau hauv qhov chaw qhuav thiab tsaus kom tsis txhob ya raws thiab nqus.
Thaum thauj, nws yuav tsum tau cim tias yog "hygroscopic khoom" los tiv thaiv kev sib cuag nrog huab cua noo.
ITPPhmoov, raws li ib tug biologically active compound, nws analytical txoj kev yuav tsum tau npog cov kev sib txuas tseem ceeb xws li kev txiav txim siab purity, structural characterization, impurity tswj thiab stability kawm. Cov nram qab no systematically txheeb tawm cov tseem ceeb analytical txoj kev ntawm ITPP Powder los ntawm peb qhov ntev: tshuaj ntsuam xyuas, ntsuas ntsuas, thiab kev txheeb xyuas cov khoom lom.
Purity txiav txim siab: xyuas kom zoo raws li cov qauv
High Performance Liquid Chromatography (HPLC)
Lub hauv paus ntsiab lus: Los ntawm kev ua kom zoo dua ntawm cov yam ntxwv tuav ntawm ITPP ntawm ib kab chromatographic tshwj xeeb, kev soj ntsuam ntau yog ua los ntawm kev sib piv nrog cov qauv tshuaj.
Kev ua kom zoo dua qub: A C18 thim rov qab- theem kem tau siv, nrog rau theem mobile yog phosphate tsis (pH 6.8) - acetonitrile (95:5), thiab qhov ntsuas wavelength yog 220 nm. Nyob rau hauv cov xwm txheej no, kev sib cais ntawm ITPP los ntawm impurities yog qhov zoo, nrog rau cov kab ntau ntawm 0.1-10 mg / mL thiab tus nqi rov qab ntawm 98% -102%.
Inductively coupled plasma Mass spectrometry (ICP{0}}MS)
Lub Hom Phiaj: txhawm rau txheeb xyuas cov hlau tsis huv (xws li txhuas, arsenic, thiab mercury) hauv ITPP.
Txoj kev: Tom qab microwave zom cov qauv, germanium (Ge) tau siv los ua tus qauv sab hauv, thiab cov ntsiab lus concentration tau txiav txim los ntawm ICP-MS.
Standard: Ua raws li cov cai ntawm Suav Pharmacopoeia lossis USP (xws li txhuas Less tshaj los yog sib npaug rau 0.1 ppm, arsenic Tsawg dua lossis sib npaug rau 0.2 ppm).
Karl Fischer txoj kev ntsuas dej noo
Lub hauv paus ntsiab lus: Raws li cov tshuaj tiv thaiv ntau ntawm iodine thiab dej, cov ntsiab lus noo noo yog txiav txim siab los ntawm titration.
Qhov xwm txheej: Methanol yog siv los ua cov kuab tshuaj, thiab qhov kawg ntawm titration yog txiav txim siab los ntawm potentiometry.
Lub ntsiab lus: ITPP yog hygroscopic, thiab cov ntsiab lus noo noo yuav tsum tau tswj tsawg dua los yog sib npaug li 1.0% los tiv thaiv degradation.
Tus yam ntxwv ntawm tus yam ntxwv: Paub meej tias molecular tus kheej thiab conformation
1H NMR: Hauv D2O, proton teeb liab ntawm cyclohexahexanol pob txha pob txha ntawm ITPP tshwm ntawm δ 3.5-4.5 ppm, thiab cov proton ntawm pyrophosphate pawg qhia tau hais tias lub ncov dav vim kev sib pauv sai.
31P NMR: Qhia cov tshuaj hloov pauv ntawm peb pawg pyrophosphate (δ -10.2, -12.5, -14.8 ppm), thiab paub meej txog hom kev sib txuas ntawm phosphate ester daim ntawv cog lus los ntawm kev sib txuas tas li.
Daim ntawv thov: Txheeb xyuas cov qauv molecular thiab conformation, thiab tshem tawm isomer cuam tshuam.
Yam ntxwv ncov
3400 cm⁻¹ (O-H stretching vibration, inositol hydroxyl);
1250 cm⁻¹ (P=O stretching vibration, pyrophosphate pawg);
1050 cm⁻¹ (P-O-C stretching vibration, phosphate ester bond).
Qhov tseem ceeb: Paub meej sai sai rau cov pab pawg ua haujlwm ntawm ITPP thiab pab txhawm rau txheeb xyuas tus qauv.
Lub hom phiaj: txhawm rau txheeb xyuas cov qauv siv lead ua ntawm ITPP.
Qhov tshwm sim: ITPP feem ntau muaj nyob rau hauv amorphous lossis semi-crystalline daim ntawv, thaum XRPD tuaj yeem tshem tawm cov crystalline impurities (xws li inositol unreacted los yog sodium pyrophosphate).
Impurity Control: Ua kom muaj kev nyab xeeb thiab muaj txiaj ntsig
Siab -kev ua haujlwm ua kua chromatography-mass spectrometry (HPLC-MS)
Lub Hom Phiaj: txhawm rau txheeb xyuas los ntawm - cov khoom lag luam thaum lub sij hawm txheej txheem synthesis (xws li monophosphate lossis inositol diphosphate derivatives).
Cov xwm txheej: A C18 kem tau siv, lub sijhawm txawb yog formic acid-acetonitrile gradient elution, thiab hom kev kuaj pom loj yog qhov tsis zoo ion electrospray (ESI-).
Qhov zoo: Nws tuaj yeem ua tiav ib txhij ua tiav kev sib cais ntawm impurity thiab kev txheeb xyuas cov qauv, nrog rau kev nkag siab txog qib ppb.
Thermogravimetric tsom xam (TGA)
Lub hauv paus ntsiab lus: Kev hloov loj ntawm cov qauv yog ntsuas los ntawm qhov programmed kub nce los txheeb xyuas thermal stability.
Qhov tshwm sim: ITPP yog qhov ruaj khov hauv qab 200 degree, thiab nws qhov decomposition kub siab tshaj 270 degree, qhia tau zoo thermal stability thiab haum rau kev lag luam synthesis.
Kev ntsuam xyuas
Txoj kev: Xa mus rau txoj kev thib ob (paddle method) ntawm Kev Txiav Txim Siab hauv Suav Pharmacopoeia, cov qauv raug coj los siv cov tshuaj phosphate buffer nrog pH 7.4 raws li qhov nruab nrab, ntawm kev sib hloov ceev ntawm 50 rpm rau 30 feeb.
Standard: ITPP dissolution tus nqi ntau dua los yog sib npaug rau 85% kom ntseeg tau tias bioavailability ntawm kev npaj.
Kev Tshawb Fawb Txog Kev Ua Txhaum Cai: Txuas Cov Khoom Siv Tshuaj thiab Kev Ua Haujlwm
Kev sim ntsuas hemoglobin allosteric kev tswj hwm
Txoj Cai:ITPP txhawb nqa oxygen tso tawm los ntawm kev txo qis oxygen affinity ntawm hemoglobin.
Txoj kev
Npaj tib neeg cov tshuaj hemoglobin (20 μM);
Ntxiv ITPP (0-100 μM) thiab ntsuas cov pa oxygen dissociation nkhaus;
Xam tus nqi P50 (qhov siab ib nrab ntawm cov pa thaum lub hemoglobin oxygen saturation yog 50%).
Qhov tshwm sim:ITPP nce tus nqi P50 los ntawm 26.5 mmHg mus rau 35.2 mmHg, lees paub nws cov kev tswj hwm allosteric.
Cell hypoxia qauv sim
Qauv:Siv CoCl2 los ua kom cov ntshav hypoxia hauv HeLa hlwb.
Kev kuaj pom
Cell viability (MTT method)
Kev nthuav qhia ntawm hypoxia-inducible factor-1 (HIF-1) (Western blot)
Lactate theem (txoj kev enzymatic).
Xaus:ITPP (100 μM) tuaj yeem txo qhov kev qhia ntawm HIF-1 thiab lactic acid txuam nrog, thiab txhim kho lub xeev hypoxia ntawm cov hlwb.
Nws qhov profile kev nyab xeeb mus ntev, kev noj zaub mov kom zoo, thiab muaj peev xwm txaus ntshai rau tib neeg tsis tau tsim kom tiav. Txawm hais tias qee qhov kev tshawb fawb tsiaj tau pom tias muaj kev ua kom muaj zog tiv thaiv thiab cov ntaub so ntswg oxygenation, cov ntaub ntawv zoo ntawm tib neeg cov ntaub ntawv kho mob tseem txwv, nrog kev qhia txog kev txhawj xeeb xws li hypotension thiab muaj peev xwm mob plawv. ITPP tsis raug pom zoo raws li kev noj zaub mov raws cai lossis kev ua kis las ntxiv, thiab nws siv los ntawm cov neeg ncaws pob tuaj yeem ua txhaum txoj cai tiv thaiv doping. Qhov kev sib xyaw no feem ntau yog muab rau kev tshawb fawb txog kev tshawb fawb, thiab ib qho uas tsis yog-lab-label daim ntawv thov ua rau muaj kev noj qab haus huv tseem ceeb thiab kev pheej hmoo raug cai uas yuav tsum tau ceev faj heev.
Cim npe nrov: itpp hmoov, lwm tus neeg, manufacturers, hoobkas, lag luam wholesale, yuav, nqe, tej, kev muag khoom