Shaanxi BLOOM Tech Co., Ltd. yog ib qho ntawm cov tuam ntxhab thiab cov muag khoom ntawm ketone ester hmoov hauv Suav teb. Txais tos rau cov lag luam wholesale bulk zoo ketone ester hmoov muag ntawm no los ntawm peb lub hoobkas. Kev pabcuam zoo thiab tus nqi tsim nyog muaj.
Ketone ester hmoovyog cov hmoov ntxiv nrog Ketone Ester ua cov khoom tseem ceeb. Nws cov ntsiab lus tshuaj yog (3R) -3-hydroxybutyl-3-hydroxybutyrate (CAS naj npawb: 1208313-97-6), nrog rau cov mis mos ntawm C8H16O4, CAS 1208313-97-6, thiab molecular hnyav ntawm 176.21. Raws li ib qho exogenous ketone lub cev ntxiv, nws ua ke - hydroxybutyric acid (BHB) nrog 1,3-butanediol los ntawm cov tshuaj esterification technology los tsim ketone precursor uas tuaj yeem nqus ncaj qha los ntawm tib neeg lub cev, tsis muaj kev cia siab rau kev noj zaub mov nruj ketogenic kom ceev cov ntshav ketone concentration.
Peb cov khoom
| Khoom npe | Ketone Ester Drops | Ketone Ester hmoov |
| Yam khoom | ua kua | Hmoov |
| Khoom Purity | Ntau dua lossis sib npaug li 99% | Ntau dua lossis sib npaug li 99% |
| Daim ntawv khoom | Rau kev siv sab nraud | Rau kev siv sab nraud |
Ketone Ester COA
 |
||
| Certificate of Analysis | ||
| Compound npe | Ketone Ester Cov | |
| Qib | Pharmaceutical qib | |
| CAS Nr. | 1208313-97-6 | |
| Ntau | 3 37,3kg | |
| Ntim txheem | 25kg / nruas | |
| Chaw tsim tshuaj paus | Shaanxi BLOOM TECH Co., Ltd | |
| Ntau No. | 202501090036 | |
| MFG | Peb 9, 2025 | |
| EXP | Peb 8, 2028 | |
| Qauv |
|
|
| Yam khoom | Enterprise txheem | Kev txheeb xyuas qhov tshwm sim |
| Qhov tshwm sim | Dawb los yog yuav luag dawb hmoov | Ua raws |
| Cov ntsiab lus dej | Tsawg dua lossis sib npaug li 5.0% | 0.38% |
| Poob rau ziab | Tsawg dua lossis sib npaug li 1.0% | 0.28% |
| Hnyav Hlau | Pb Tsawg dua lossis sib npaug li 0.5ppm | N.D. |
| Tsawg dua lossis sib npaug li 0.5ppm | N.D. | |
| Hg Tsawg dua lossis sib npaug li 0.5ppm | N.D. | |
| Cd Tsawg dua lossis sib npaug li 0.5ppm | N.D. | |
| Purity (HPLC) | Ntau dua lossis sib npaug li 99.0% | 99.80% |
| Ib leeg impurity | <0.8% | 0.45% |
| Tag nrho microbial suav | Tsawg dua lossis sib npaug li 750cfu / g | 90 |
| E. Coli | Tsawg dua lossis sib npaug li 2MPN / g | N.D. |
| Salmonella | N.D. | N.D. |
| Ethanol (los ntawm GC) | Tsawg dua lossis sib npaug li 5000ppm | 400ppm ua |
| Cia | Khaws rau hauv qhov chaw kaw, tsaus, thiab qhuav hauv qab -20 degree | |
|
|
||
|
|
||
Ketone ester hmoovyog ib hom organic compound tsim los ntawm cov tshuaj esterification ntawm - hydroxybutyric acid (BHB) nrog luv saw alcohols (xws li 1,3-butanediol). Lawv cov txheej txheem tseem ceeb ntawm kev ua haujlwm yog kom ceev cov ntshav keton concentration los ntawm exogenous supplementation ntawm keton lub cev, yog li txhim kho lub zog mov, neuroprotection, thiab kev tswj cov kab mob metabolic los ntawm ntau qhov kev tswj hwm metabolic.
1. Hydrolysis kinetics thiab absorption efficiency ntawm ester bonds
Cov khoom tseem ceeb (xws li D-BHB-R-1,3-butanediol monoester) yog hydrolyzed sai los ntawm esterases hauv txoj hnyuv, tso tawm dawb BHB thiab butanediol. Cov txheej txheem no hla cov kauj ruam ib txwm muaj ntawm ketogenic acid dissociation, tso cai rau cov ntshav keton concentration siab tshaj 1.5mmol / L pib hauv 15 feeb, ua rau 300% nce hauv kev nqus tau zoo piv rau ketogenic ntsev. Kev tshawb fawb ntawm Oxford University qhia tau hais tias 25g ketoester tee tuaj yeem ua rau cov ntshav siab tshaj plaws keton concentration rau 3.8mmol / L, uas nyob ze rau qib keton lub cev tom qab 72 teev ntawm kev yoo mov.
2. Optimization ntawm mitochondrial zog metabolism
Dawb BHB nkag mus rau mitochondria los ntawm monocarboxylate transporter (MCT1/2) thiab yog catalyzed los ntawm - hydroxybutyrate dehydrogenase los tsim acetyl CoA. Cov txheej txheem no ua rau 22.5ATP / molecule, uas yog 28% ua haujlwm zoo dua li cov piam thaj metabolism, thaum txo cov cim ntawm cov pa oxygen reactive (ROS) los ntawm 70%, tsim "lub zog huv" qauv. Nyob rau hauv ib puag ncig hypoxic (xws li qhov siab- kev cob qhia qhov siab), BHB metabolism tuaj yeem hla cov kauj ruam pyruvate carboxylase, tswj ATP synthesis tus nqi, thiab txo qis lactate tsub zuj zuj ntawm 41%.
3. Neuroprotection thiab teeb liab transduction
BHB tsis yog tsuas yog lub zog substrate, tab sis kuj yog ib qho kev taw qhia molecule:
Kev tswj hwm Epigenetic: inhibits HDAC enzyme kev ua, txhawb nqa BDNF noob qhia, thiab nce synaptic ceev ntawm hippocampal neurons los ntawm 300%.
Antioxidant tiv thaiv: Ua kom txoj hauv kev Nrf2, txhim kho glutathione synthesis (2.5-fold nce hauv antioxidant muaj peev xwm), thiab txo thaj tsam ntawm A plaques hauv hlwb ntawm Alzheimer tus kab mob qauv nas los ntawm 27%.
Kev tswj hwm neuroexcitability: Thaiv kev ua kom ntau dhau ntawm NMDA receptors txo qis qaug dab peg los ntawm 67%, thaum txhim kho GABAergic neurotransmission thiab txhim kho kev ntxhov siab xws li kev coj cwj pwm.
Ketone ester hmoovyog ib hom organic compound tsim los ntawm cov tshuaj sib txuas ntawm ketons (xws li - hydroxybutyric acid, BHB) nrog cawv. Nws cov qauv tseem ceeb yog R1C (=O) R2C (=O) OR3, qhov twg R1, R2, thiab R3 yog alkyl lossis pawg ua haujlwm. Raws li ib qho exogenous keton lub cev ntxiv, cov synthesis ntawm keton esters yuav tsum muaj qhov sib npaug ntawm cov tshuaj tiv thaiv, cov khoom purity, thiab bioavailability.
Txoj kev siv tshuaj lom neeg: txoj hauv kev tseem ceeb ntawm cov tshuaj tiv thaiv esterification
Cov tshuaj synthesis yog cov txheej txheem tseem ceeb rau kev tsim khoom ntawm keton esters, thiab nws cov tshuaj tiv thaiv tseem ceeb yog esterification lossis ester pauv cov tshuaj tiv thaiv ntawm carboxylic acid thiab cawv, uas yuav tsum tau hloov pauv tau zoo los ntawm catalysts, cov kuab tshuaj, thiab kev tswj kub.
1. Tsob tshuaj acid catalyzed esterification txoj kev
Reaction txoj cai: Carboxylic acid thiab cawv nyob rau hauv lub cev qhuav dej condensation nyob rau hauv acidic tej yam kev mob, tsim esters thiab dej. Noj cov synthesis ntawm tert butyl ester ua piv txwv, carboxylic acid (xws li (3R) -3-hydroxybutyric acid) thiab tert butanol yog catalyzed los ntawm concentrated sulfuric acid thiab nplawm hauv cov roj da dej ntawm 60℃rau 12 teev. Cov pab pawg hydroxyl ntawm carboxylic acid yog hloov los ntawm tert butoxy pawg los tsim tert butyl ester.
Cov ntsiab lus tseem ceeb:
Molar piv: carboxylic acid: tert butanol: concentrated sulfuric acid =1: 3: 0.5, tshaj tert butanol tuaj yeem thawb cov tshuaj tiv thaiv sib npaug mus rau sab xis.
Kev tswj qhov kub thiab txias: Lub boiling point ntawm tert butanol yog 82 degree, thiab nws yuav tsum tau txuas nrog ib tug reflux condenser los tiv thaiv volatilization; Qhov kub siab tshaj 70℃tuaj yeem ua rau lub cev qhuav dej, ua rau olefins lossis tar.
Tom qab kev kho mob: Tom qab cov tshuaj tiv thaiv tiav, cov kua qaub ntau dhau yuav tsum tau nruab nrog sodium bicarbonate. Tom qab sib cais, cov theem ntawm cov organic yog qhuav dhau anhydrous sodium sulfate thiab purified los ntawm lub tshuab nqus tsev distillation.
Case: Lub chaw tsim tshuaj siv cov kev cai no los ua qauv tsim cov tshuaj aspirin derivative intermediates. Los ntawm ua ntej txias cov tshuaj tiv thaiv rau -10℃thiab maj mam cua sov nws, cov tawm los tau nce los ntawm 75% mus rau 88%.
2. DCC/DMAP coupling txoj kev
Reaction txoj cai: Dicyclohexylcarbodiimide (DCC) yog siv los ua lub cev qhuav dej kom ua kom cov carboxylic acid carboxyl pawg; 4-Dimethylaminopyridine (DMAP) yog siv los ua ib qho catalyst los txhawb kev tawm tsam ntawm oxygen anions los ntawm cawv ntawm activated carboxyl pawg, uas ua rau tsim cov esters. Txoj kev no yog tsim rau acid rhiab substrates, xws li cov tebchaw uas muaj phenolic hydroxyl pawg.
Cov kauj ruam ua haujlwm:
Dissolve carboxylic acid, tert butanol, DCC (1.2 sib npaug), thiab DMAP (catalytic npaum li cas) hauv anhydrous dichloromethane thiab do thaum hmo ntuj ntawm chav tsev kub.
Lim thiab tshem tawm cov generated dicyclohexylurea (DCU) precipitate, purify cov organic theem los ntawm silica gel kem chromatography, thiab siv roj av ether / ethyl acetate (ntim piv 3: 1) ua tus tsim tus neeg sawv cev.
Qhov zoo thiab kev txwv:
Qhov zoo: Cov tshuaj tiv thaiv me me, cov txiaj ntsig siab (txog 90% lossis ntau dua), tsim nyog rau kev hloov kho molecular complex.
Kev txwv: DCC yog cov tshuaj lom heev thiab yuav tsum muaj kev tiv thaiv nruj; DMAP muaj tus nqi siab dua, uas ua rau cov nqi nce ntxiv.
Lwm txoj kev daws teeb meem:
EDC / HOBt txoj kev: Siv 1-ethyl - (3-dimethylaminopropyl) carbodiimide hydrochloride (EDC) thiab 1-hydroxybenzotriazole (HOBt) es tsis txhob DCC / DMAP, cov txiaj ntsig tau txo qis li 5%, tab sis cov tshuaj toxicity tau txo qis.
Ionic kua catalysis: Siv [Bmim] HSO4 ionic kua siv cov concentrated sulfuric acid tuaj yeem siv tau ntau dua 5 zaug, nrog cov txiaj ntsig zoo piv rau sulfuric acid txoj kev, tab sis nrog cov cuab yeej siv nyiaj ntau dua.
3. Esterification txoj kev: hloov los ntawm methyl ester mus rau lub hom phiaj ester
Reaction txoj cai:Siv cov cawv esters qis (xws li methyl esters) raws li cov khoom siv raw, lawv tau txais cov tshuaj ester pauv nrog lub hom phiaj cawv nyob rau hauv alkaline tej yam kev mob los ua kom muaj cawv ntau dua esters thiab methanol. Piv txwv li, siv (3R) -3-hydroxybutanoate methyl ester thiab 1,3-butanediol ua raw cov ntaub ntawv, nyob rau hauv lub catalysis ntawm sodium methoxide, cov tshuaj tiv thaiv yog nqa tawm ntawm 80℃rau 6 teev los tsim (3R) -3-hydroxybutyl-3-hydroxybutanoate ester (core qauv ntawm keton ester).
Catalyst ntau npaum:Kev noj tshuaj sodium methoxide yog 5% ntawm tag nrho cov molar tus nqi ntawm cov substrate, thiab ntau npaum li cas tuaj yeem ua rau muaj kev phiv xws li cawv.
Kev xaiv cov kuab tshuaj:Siv cov toluene raws li cov kuab tshuaj tuaj yeem ua rau kom muaj kev sib haum xeeb ntawm cov tshuaj reactants thiab txhawb methanol evaporation, yog li txhawb cov tshuaj tiv thaiv equilibrium.
Purification txheej txheem:Cov tshuaj tiv thaiv yog ntxuav nrog dej kom tshem tawm cov catalyst, qhuav nrog anhydrous sodium sulfate, thiab tom qab ntawd raug rau lub tshuab nqus tsev distillation kom sau cov 150-155℃feem (siab 2mmHg).
Case:Ib lub tuam txhab tau txais cov qauv no los tsim cov keton ester intermediates, thiab txhim kho cov purity ntawm cov khoom mus rau 99% los ntawm cov txheej txheem distillation tas li, nrog rau ib qho batch yield ntawm 50kg.
Txoj kev siv tshuaj lom neeg: ib qho kev taw qhia tshiab rau kev tsim ntsuab
Kev qhia ncaj qha ntawm keton esters los ntawm enzymatic catalysis lossis microbial metabolism hauv biosynthesis muaj qhov zoo ntawm cov mob me thiab kev xaiv siab, tab sis nws tseem nyob rau hauv kev tshawb fawb theem.
1. Enzymatic esterification
Reaction txoj cai: Siv lipase (xws li Candida Antarctica lipase B, CALB) ua ib qho catalyst, nws catalyzes cov tshuaj tiv thaiv esterification ntawm carboxylic acids thiab cawv hauv cov kuab tshuaj organic los yog aqueous theem. Piv txwv li, siv (3R) -3-hydroxybutyric acid thiab 1,3-butanediol ua substrates, reacting nyob rau hauv tert butanol ntawm 40℃rau 24 teev ua keton esters nrog ib tug conversion tus nqi ntawm 85%.
Qhov zoo:
Stereoselectivity: Enzymes tuaj yeem paub txog cov txheej txheem substrate thiab tsim ib qho khoom enantiomeric (xws li R-configured ketoesters).
Environmentally friendly: Tsis txhob siv cov tshuaj lom catalysts, cov tshuaj tiv thaiv me me.
Kev txwv:
Tus nqi Enzyme: Kev lag luam lipase muaj tus nqi siab, uas txwv nws cov ntawv thov loj -.
Substrate concentration: High substrate concentration tau yooj yim ua rau enzyme inactivation, thiab cov tshuaj tiv thaiv yuav tsum tau optimized.
2. Microbial Metabolic Engineering
Cov txheej txheem tshuaj tiv thaiv: Cov kab mob (xws li Escherichia coli thiab poov xab) tau hloov kho los ntawm cov noob kho tshuab los qhia txog keton lub cev synthase systems (xws li HMG CoA synthase thiab HMG CoA lyase) thiab ester synthase, ua tiav tag nrho cov cell catalysis ntawm qabzib mus rau keton esters. Piv txwv li, co qhia acetyl CoA carboxylase, acetyl CoA thiolase, thiab ester synthase hauv Escherichia coli, siv cov piam thaj los ua cov pa roj carbon monoxide, tuaj yeem tsim ketoesters mus rau 1.2g / L tom qab 72 teev ntawm fermentation.
Kev sib tw:
Metabolic flux:Ketone ester hmoovNws yog ib qho tsim nyog kom sib npaug ntawm cov khoom ua ntej thiab cov khoom toxicity kom tsis txhob muaj kev loj hlob ntawm tes.
Kev sib cais ntawm cov khoom: Lub fermentation broth muaj complex Cheebtsam thiab yuav tsum tau tsim kom muaj kev sib cais txheej txheem xws li molecular distillation thiab crystallization.
Kev siv Ketone Ester Powder hauv Microbial Metabolism
Ketone ester hmoov feem ntau yog siv hauv microbial metabolic engineering los tswj cov ketone metabolism hauv intracellular, txhim kho cov khoom siv hluav taws xob, thiab txhim kho cov khoom sib txuas. Lub hauv paus tseem ceeb yog txhawm rau nce qib hauv cov cell sai sai ntawm - hydroxybutyric acid ( - OHB) thiab lwm lub cev ketone los ntawm exogenous supplementation, yoog raws li cov txheej txheem metabolic reprogramming ntawm cov kab mob engineering. Cov hauv qab no yog cov txheej txheem thov tshwj xeeb, cov tswv yim ua haujlwm, thiab cov lus qhia txog rooj plaub:
Hais txog kev txhim kho ntawm lub zog metabolism
Lub tshuab yog los ua lub zog muaj zog substrate, muab lub zog rau cov kab mob engineering hauv qab cov piam thaj lossis cov pa oxygen, txo cov metabolism hauv lub cev los ntawm kev tsis txaus ATP.
Nws tuaj yeem siv los txhim kho kev sib xyaw ua ke ntawm siab -cov khoom siv hluav taws xob xws li fatty acids, polyhydroxyalkanoates (PHA), terpenes, thiab lwm yam; Txhim kho qhov kev ntxhov siab ntawm cov kab mob engineered hauv siab - ntom fermentation.
Muab cov khoom ua ntej rau cov khoom sib txuas
Lub tshuab yog tias - OHB tsim los ntawm hydrolysis ntawm ketone esters tuaj yeem ua tus thawj coj thiab hloov mus rau hauv cov nruab nrab tseem ceeb xws li acetyl CoA thiab - ketoadipic acid los ntawm enzymatic catalysis.
Nws tuaj yeem siv rau hauv kev sib txuas ntawm polyketide compounds, aromatic compounds, thiab biobased monomers (xws li - ketoadipic acid).
Cim npe nrov: ketone ester hmoov, lwm tus neeg, manufacturers, hoobkas, lag luam wholesale, yuav, nqe, tej, kev muag khoom