Lactoferrin ntsiav tshuajYog kev noj haus ntxiv lossis kev npaj ua lag luam tsim nrog lacoferrin li cov khoom siv tseem ceeb. LactoFerrin yog ib qho kev sib xyaw ua ke glycoproedin lawm nyob rau hauv cov mis nyuj ntawm cov tsiaj yug menyuam, teej tug rau tsev neeg hloov chaw. Nws muaj ntau cov roj ntsha ua haujlwm xws li cov tshuaj tua kab mob, tiv thaiv kev tiv thaiv, thiab txhawb cov kev nqus hlau. Cov khoom lag luam no kuj tseem tuaj yeem muaj lwm cov tshuaj xyaw, xws li lactose, hmoov txhuv nplej siab, thiab lwm yam, txhawm rau txhim kho nws cov ruaj khov thiab cov xyoob ntoo.
Raws li tib neeg lub siab rau kev noj qab haus huv thiab khoom noj khoom haus tseem nce, qhov kev thov rau cov ntsiav tshuaj ntxiv lossis siv tshuaj yeeb, yog maj mam nce hauv kev ua lag luam. Tam sim no, muaj cov khoom lag luam ntawm ntau hom npe ntawm kev ua lag luam, thiab kev sib tw tau dhau los ua tsiv. Yav tom ntej, nrog kev nce qib nruam kev tshawb fawb thiab thev naus laus zis thiab cov khoom lag luam sib txawv ntawm cov neeg tau txais kev pabcuam, cov khoom no yuav them ntau dua rau kev txhim kho kom zoo, kev nyab xeeb thiab ua haujlwm zoo. Lub caij no, cov khoom lag luam uas tau muab rau cov neeg sib txawv ntawm cov tib neeg thiab lawv cov kev xav tau kuj tseem yuav dhau los ua tus qauv tseem ceeb hauv kev ua lag luam.
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Lactoferrin hmoov coa
Lactoferrin ntsiav tshuajyog hom kev npaj uas muaj lactoferrin (LF) raws li cov khoom siv tseem ceeb. LactoFerrin yog ib tug hlau -}} binding glycoplein nrog cov tshuaj tua tsiaj, xws li antibrulatory thiab txhawb nqa hlau nqus. Thaum tshawb xyuas cov khoom no, ntau txoj hauv kev yuav tsum tau saws kom ntseeg tau tias nws cov ntsiab lus, kev coj dawb huv thiab kev ua si. Kev tsom xam txheej ntawm nws yuav nthuav dav hauv qab no.
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Cov Ntsiab Lus Kev Txiav Txim Siab
Kev ua kom zoo ua kua chromatography (HPLC)
HPLC yog ib txoj kev sib txawv rau kev txiav txim siab cov ntsiab lus ntawm lactoferrin. Lub ntsiab cai yog muab cais lacoferrin los ntawm lwm cov kev cuam tshuam los ntawm kev faib cov khoom sib txawv ntawm cov tshuaj sib txawv ntawm cov theem nyob sib txawv ntawm theem thiab cov mobile theem. HPLC nta cov siab rhiab siab, muaj qhov tseeb thiab kev ua kom muaj txiaj ntsig zoo, thiab yog siv ntau yam nyob rau hauv cov lacoferrin.
Cov kauj ruam ua haujlwm:
Qauv tshwm sim: yaj covLACTOFERRIN COV KHWVHauv kev daws teeb meem tsis tsim nyog, centrifuge kom tshem tawm insoluble cov tshuaj, thiab coj tus supernatant rau kev txiav txim siab.
Chromatographic mob: Ib qho kev thim rov qab -} theem theem khe cov kuab tshuaj thiab cov buffers, nrog gradient elient.
Nrhiav kom tau txais: UV NTES siv, nrog kev nrhiav wavelength li ntawm 280 nm. Cov ntsiab lus ntawm LACTOFRIN yog suav raws cov nkhaus txheem.
Enzyme txuas immunosorbent assay (Elisa)
Elisa yog tus qauv immunological raws li cov tshuaj tiv thaiv ntawm cov tshuaj pleev thiab cov tshuaj tiv thaiv kev sib txawv, thiab haum rau cov lactoferrin.
Cov kauj ruam ua haujlwm:
Cov txheej: cov tshwj xeeb anti - lacteferrin antibody yog coated ntawm micpplate.
Kev thaiv: Ntxiv cov thaiv cov tshuaj kom tiv thaiv tsis yog -}} Tshwj xeeb khi.
Qauv Tsim Txom: Ntxiv cov qauv uas yuav tsum tau kuaj xyuas thiab ua rau cov tshuaj tiv thaiv kab mob.
}}}}}}}}}}}}}}}}} Ant ant ant ant} ant ant ant ant ant ant ant ant ant ant ant ant ant ant ant ant ant ant ant ant ant ant ant ant antibody complex.
Cov tshuaj tiv thaiv xim: ntxiv cov kev daws teeb meem los tsim cov tshuaj tiv thaiv xim, thiab ntsuas cov nqus ntawm lub yoj yoj tshwj xeeb.
Xam: Raws li kev nkhaus txheem, laij cov ntsiab lus ntawm LACTOFRIN hauv cov qauv.
Purity tsom xam
Kev ua kom zoo ua kua chromatography Mow spectrometry (HPLC - ms)
HPLC - ms sib xyaw ua ke ntawm cov checomyography, thiab muaj peev xwm txiav txim siab yog kev txiav txim siab lub purity thiab molecular qhov hnyav ntawm lacoferrin.
Cov kauj ruam ua haujlwm:
Qauv Qauv Pretreatment: tib yam li HPLC txoj kev.
Chromatographic sib cais: Siv ib qho tsim nyog chromatographic kem thiab mobile theem cais cais lactererrin.
Mass Spectrometry Kev Tshawb Xyuas: Cov Spectrometry Kev Tshawb Xyuas yog ua rau LACTOFER LACTOFRIN los txiav txim siab nws txoj kev nruj me me thiab txoj kev huv.
Capillary electrophoresis (CE)
Ce yog kev sib cais thev naus laus zis raws li qhov sib txawv hauv ion
Cov kauj ruam ua haujlwm:
Qauv npaj: yaj cov ntsiav tshuaj thiab centrifuge kom tshem tawm impurities.
Electrophoresular Culture: Xaiv ib qho tsim nyog tsis haum thiab voltage rau electrophorisation sib cais.
Nrhiav kom tau paub: Siv tus nqi UV los sau cov qauv hluav taws xob thiab tshawb xyuas cov kev coj dawb huv ntawm lactoferrin.
Kev Ntsuas Yam Ntxim Taw
Kev txiav txim siab ntawm cov hlau khi muaj peev xwm
Cov hlau -} binding muaj peev xwm ntawm lacelerrin yog ib qho ntawm nws cov kev ua ub no tseem ceeb thiab tuaj yeem txiav txim siab los ntawm tus hlau saturation txoj kev.
Cov kauj ruam ua haujlwm:
Hlau Saturation: Tsim LactoFerrin nrog ib qho dhau ntawm cov hlau ions kom ua tiav lub xeev ntawm cov hlau saturation.
Cov Hlau Tso Tawm Dawb: Unbound Dawb Hlau tau muab tshem tawm los ntawm centrifugation lossis ultrafiltration.
Xim cov tshuaj tiv thaiv: ntxiv cov xim tsim tawm, txiav txim seb tus nqi ntawm ciam ntawm cov hlau, thiab suav cov hlau khi muaj peev xwm ntawm lactoferrin.
Kev txiav txim siab ntawm cov tshuaj tua kab mob antibacterial
Lactoferrin muaj cov tshuaj tua kabmob thiab tuaj yeem txiav txim siab los ntawm thaj chaw inhibition ntawm txoj kev lossis qhov tsawg kawg nkaus inhibitions conhibitions (mic).
Cov kauj ruam ua haujlwm:
Inhibition Zov Qauv: Ntxiv cov qauv muaj lactoferrin mus rau agar phaj uas muaj qhov ntsuas thiab saib qhov tsim ntawm thaj chaw inhibition.
Cov kev txiav txim siab no: Lactoferrin yog diluted nyob rau hauv ib qho nruab nrab uas muaj qhov ntsuas ntsuas ntsuas qhov ntsuas qhov tsawg kawg uas muaj cov kab mob loj hlob.
Kev Ntsuas Cov Qauv
Vas Dichroism (CD)
CD tuaj yeem siv los tshuaj xyuas tus qauv txheej txheem theem nrab ntawm lacoferrin thiab nkag siab txog kev hloov pauv hloov hauv cov ntsiav tshuaj.
Cov kauj ruam ua haujlwm:
Qauv npaj: yaj cov ntsiav tshuaj thiab dilute nws mus rau qhov tsim nyog.
Kev txiav txim siab: Siv ib qho kev sib tw ntawm Dichrics Chromatograph rau scratograph rau scratogrograph rau ntawm nthwv dej ntau ntawm 190-260 nm thiab txheeb xyuas qhov muaj pes tsawg leeg ntawm cov qauv theem nrab.
Fourier Transform Infrared Spectroscopy (FTIR)
Ftir tuaj yeem siv los txheeb xyuas cov kev sib txuas lus thiab ua haujlwm ntawm lacoferrin thiab nkag siab nws cov qauv kev teeb tsa.
Cov kauj ruam ua haujlwm:
Qauv npaj: sib xyaw LACTOFRIN nrog KBR thiab nias rau hauv cov ntsiav tshuaj.
Kev txiav txim: Siv ib qho kev ntsuas infrared mus rau scan tus qauv thiab txheeb xyuas cov spectrum infrared.
Kev ua xua
LACTOFERRIN COV KHWVyog hom khoom noj khoom haus tsuas yog muaj ntawm lacoferrin, uas muaj cov haujlwm ntawm kev tiv thaiv kev tiv thaiv thiab txhawb nqa hlau nqus. Txawm li cas los xij, lactoferrin, raws li cov protein, ua rau muaj protein, tuaj yeem ua xua ua xua thiab ua rau muaj qee yam kev pheej hmoo ntawm kev fab tshuaj. Cov hauv qab no yuav ua cov tshuaj ntsuam ntxaws ntxiv los ntawm cov kev tshuaj xyuas uas muaj kev phom sij, cov tsos mob ua rau pom, thiab kev tiv thaiv kev ntsuas, thiab kev tiv thaiv kev ntsuas.
Kev ua xua
Cov neeg uas muaj kev fab tshuaj tsis haum muaj cov rhiab heev rau LACTOFRIN, thiab tsev neeg zaj keeb kwm ntawm kev ua xua kev ua xua. Thaum lub cev tsis muaj zog lamsoferrin ua antigen antigen, nws yuav ua rau muaj lub cev tsis muaj zog ntau dhau, ua rau muaj kev fab tshuaj tiv thaiv. Ib qho ntxiv, cov neeg xws li cov menyuam mos thiab cov menyuam yaus thiab cov neeg laus, uas tsis muaj zog tsim los yog muaj kev phom sij ntau dua, muaj kev pheej hmoo siab ntawm kev ua xua.
Thaum lub sijhawm cov txheej txheem ntau lawm ntawm cov khoom lag luam no, yog tias cov kev coj dawb huv tsis raug tswj hwm lossis muaj lwm cov protein impurities, nws yuav ua tsis tau muaj kev ua xua. Antigenic tshuaj nyob rau hauv impurities tuaj yeem txhawb lub cev tiv thaiv kab mob thiab nce kev pheej hmoo ntawm kev ua xua. Yog li ntawd, nws yog qhov tseem ceeb tshaj plaws los xaiv cov khoom lag luam tsim los ntawm cov tuam ntxhab ib txwm thiab ntawm kev ntseeg tau zoo.
Ntau dhau ntawm lacoferrin yuav nce qhov tshwm sim ntawm kev ua xua, tshwj xeeb tshaj yog thaum thawj zaug raug. Ib qho ntxiv, qhov sib txawv ntawm kev pheej hmoo ntawm kev nqos cov ntsiav tshuaj ncaj qha thiab noj tom qab dissolving tseem xav tau kev tshawb fawb ntxiv. Txawm li cas los xij, nws raug nquahu kom ua raws li cov tshuaj thiab cov thawj coj tswj hwm qhia txog cov lus qhia khoom lossis cov kws kho mob qhia.
Cov tsos mob tsis haum
Tom qab raug lactoferrin, cov tsos mob xws li cov nqaij khaus khaus, erythema, washes thiab pob khaus. Thaum muaj mob hnyav, nws yuav tsim kho rau urticaria lossis eczema. Cov tsos mob no feem ntau tshwm sim tsis pub dhau ib feeb mus rau cov xuab moos tom qab kev sib cuag tom qab kev sib cuag thiab tuaj yeem raug kaw rau lub vev xaib lossis kis thoob plaws lub cev.
Hnoos, hawb pob thiab ua pa nyuaj yuav tshwm sim. Thaum muaj mob hnyav, tej zaum nws yuav ua rau kom muaj kev ntxhov siab. Cov tsos mob zoo li no qhia tau tias tsis haum tshuaj tau cuam tshuam rau cov ua pa kev ua pa mucosa, uas tuaj yeem ua rau lub pob txha caj qaum. Yuav tsum muaj kev kho mob tam sim ntawd.
Cov plab zom mov tsis zoo xws li xeev siab, ntuav, mob plab thiab raws plab yuav tshwm sim. Cov tsos mob ntawm lub plab zom mov yuav tshwm sim ib leeg lossis nruab nrab nrog cov ntawm lwm lub tshuab. Ntau dhau ntawm LACTOFRIN kuj tseem tuaj yeem ua rau kem plab, xws li tsam plab thiab cem quav.
Liab, o, ua kua muag, khaus lossis kev hnov qhov muag pom hauv lub qhov muag yuav tshwm sim. Cov tsos mob no muaj kev sib haum xeeb ntawm lub qhov muag rau lacoferrin, uas yuav ua rau lub zeem muag los yog tsis xis nyob.
Kub taub hau, qaug zog, kiv taub hau thiab txawm anaphylactic kev poob siab yuav tshwm sim. Systemic Kev sib raug zoo yog mob thiab cov neeg muaj mob hnyav uas yuav tsum tau kho lub neej thaum muaj xwm ceev xws li kev txhaj tshuaj maj mam epinephrine.
Kev ua xua kev pheej hmoo
Kev Ntsuas Keeb Kwm
Ua ntej noj nws, ib qho yuav tsum muaj kev nkag siab ntawm tus kheej thiab tsev neeg ib qho kev ua txhaum cai thiab ua xua rau cov zaub mov xws li mis protein. Yog tias muaj keeb kwm ntawm kev ua xua,LACTOFERRIN COV KHWVyuav tsum tau siv nrog kev ceev faj. Kev ua xua yog yuav tsum tau ua yog tias tsim nyog.
Kev ua xua
Muaj kev xua LACTOFRINA tuaj yeem lees paub los ntawm kev kuaj cov tawv nqaij prick lossis ntshav tshwj xeeb ige ige nrhiav kom tau. Yuav tsum tau ua pov thawj pov thawj cov zaub mov noj tuaj yeem ua tau thaum tsim nyog, tab sis lawv yuav tsum tau nqa tawm hauv kev saib xyuas mob kom tsis txhob muaj kev tsis haum tshuaj.
Ua raws li tus kws kho mob cov lus qhia
Thaum siv nws thawj zaug, pib nrog koob tshuaj tsis tshua muaj siab thiab pom lub cev teb. Yog tias ib qho tsis txaus ntseeg tshwm sim, tsis txhob siv nws tam sim ntawd thiab nrhiav kev kho mob. Rau cov neeg uas tau kuaj pom tias yog tsim nyog kom zam rau kev ua xua, nws cov khoom lag luam, suav nrog cov hmoov mis thiab cov khoom noj muaj txiaj ntsig muaj lactoferrin.
Lwm txoj kev daws teeb meem
Cov uas ua xua rau lacroferrin tuaj yeem xaiv cov khoom noj muaj txiaj ntsig protein zoo li, xws li kua protein thiab taum mog. Nws yog ib qho tsim nyog los them sai sai rau qhov sib txawv hauv cov khoom noj muaj kev noj haus ntawm cov khoom noj thiab lactoferrin. Yog tias tsim nyog, sab laj hais tias tus neeg noj zaub mov kom muaj kev noj zaub mov zoo.
Xaiv cov khoom thiab khaws cia
Xaiv cov khoom lag luam tsim tawm los ntawm cov chaw tsim khoom lag luam thiab kev ntseeg siab kom tsis txhob muaj cov khoom lag luam uas tsis paub txog lub teeb, nws yog qhov ua tau zoo ntawm lub teeb, nws yog qhov ua pov thawj thiab noo noo- ntawv pov thawj. Zam kev kub siab lossis huab cua huab cua uas yuav ua rau cov khoom lag luam rau tsis zoo thiab nce kev pheej hmoo ntawm kev ua xua.
Npaj Thaum Muaj Xwm Ceev
Txog siab - Cov pab pawg muaj kev pheej hmoo, nws raug nquahu kom nqa tawm los tiv thaiv (xws li Loratadine, thiab lwm yam) thiab lwm yam thaum muaj xwm txheej ceev. Nyob rau tib lub sijhawm, paub txog cov chaw ntawm cov tsev kho mob nyob ze kom koj tuaj yeem nrhiav kev kho mob kom haum thaum muaj kev tsis haum tshuaj uas hnyav.
Jak - cov ntsiab lus ntawm txoj kev
Lub Jak - cov ntsiab lus piav qhia ntawm c cytokine ntawm lub cev muaj peev xwm xws li kev sib txawv ntawm tes, kev sib txawv, lub plab zom mov, thiab cov lus teb. Thaum cytokines khi rau cov receptors ntawm cov xov tooj ntawm tes, cov receptors dhau los ntawm kev txom nyem dhau los, ua kom lub Jak Kinase uas khi rau lawv. Jak Kinase tuaj yeem phosphorylate tyrosine cov seem ntawm cov receptors, kev sib sau cov statin cov chaw. Stat (signal transducer thiab activator ntawm cov ntawv sib txuas) tuaj yeem khi rau phosphorylated tyrosine cov khoom seem, uas yog tom qab ntawd phosphorylated los ntawm jak Kinases. PhosphoralLated cov hmoov protein daim ntawv dimers, nkag rau lub nucleus, thiab khi rau cov hom phiaj ntawm cov hom phiaj ntawm cov phiaj xwm kev tiv thaiv kab mob.
Jak - cov ntsiab lus ntawm txoj kev
Qhov ncauj lactoferrin tuaj yeem cuam tshuam txog kev sib txawv thiab kev ua haujlwm ntawm kev tiv thaiv kab mob los ntawm kev ua haujlwm ntawm Jak {{l} l {stat signaling txoj kev. Piv txwv li, lacoferrin tuaj yeem ua kom lub jak1 - statg3 kev piav qhia txoj kev sib txawv ntawm th17 hlwb; Lub caij no, LACTOFRIN tuaj yeem qhib lub Jak2-stat5 kos npe rau txoj kev, txhawb nqa kev sib txawv ntawm Treg cell. Kev tshawb fawb tau qhia tias qhov kev coj ua ntawm lactoferrin tuaj yeem nce nce qib phosphorylated Jak2 thiab Statited thiab sib txawv, thiab txhim kho lawv cov kev tiv thaiv kev tiv thaiv kab mob; Nyob rau tib lub sijhawm, lactoferrin kuj tseem tuaj yeem txo cov qib phosphorholated Jak1 thiab Stat3 hauv th17 lub hlwb, thiab txo IL-17 kev tsim khoom. Tsis tas li ntawd, qhov ncauj lactoferrin tseem tuaj yeem tswj hwm lub jak-stat signaling txoj kev, cuam tshuam rau b c cell proliferation thiab antibody zais cia, thiab txhawb kev tiv thaiv kev tiv thaiv kabmob byporal. Piv txwv li, lacoferrin tuaj yeem ua kom lub Jak2-stat5 kos npe rau kev nthuav dav thiab sib txawv ntawm B Cell rau hauv Plasma Intomody.
Tgf - / smad signaling txoj kev
Lub tgf - / smad signaling txoj kev yog ib qho kev tiv thaiv kev ua kom muaj zog thiab kev sib txawv, tshuaj tiv thaiv kab mob, thiab tiv thaiv kab mob. Tgf - yog txoj kev sib tw ntawm tes, sib sau ua ke thiab ua kom cov kev ua si Kinease ntawm Tr ⅰ. Tr ⅰ tuaj yeem phosphorylate smad2 thiab smad3 protein, thiab smad rawm ntawm lub hom phiaj ntawm lub hom phiaj ntawm lub hom phiaj ntawm kev tiv thaiv kev tiv thaiv kev tiv thaiv thiab mob siab teb.
Tgf - / smad signaling txoj kev
Cov thawj coj ntawm lacrefrin tuaj yeem ua kom lub tgf - / supad signaling txoj kev sib txawv, txhawb nqa qhov sib txawv thiab kev tiv thaiv kev tiv thaiv kev sib txawv ntawm Treg Cells. Kev tshawb fawb tau qhia tias kev tswj hwm qhov ncauj tuaj yeem nce qib ntawm SMAD2 thiab SMAD3, txhawb nqa cov lus qhia ntawm Treg Medicals cov khoom seem (xws li FOXP3) thiab Txhawb txoj kev sib txawv thiab muaj txiaj ntsig ntawm Treg hlwb. Meanwhile, qhov ncauj lactoferrin kuj tseem tuaj yeem qhib lub tgf- Piv txwv li, hauv tus nas qauv ntawm autoimmune encephalyelitis (EAE}
Cim npe nrov: lactoferrin ntsiav tshuaj, tus neeg muag khoom, chaw tsim khoom, Hoobkas, lag luam wholesale, yuav, nqe, loj, loj, rau muag